Changes in Fruit Phenolic Composition and Antioxidant Capacity during Storage

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 469b-469 ◽  
Author(s):  
W. Kalt ◽  
C.F. Forney ◽  
J. McDonald

Ripe fruit of strawberry, raspberry, and highbush and lowbush blueberries were stored at 0, 10, 20, or 30 °C for up to 8 days. Total phenolic and anthocyanin content was measured at regular intervals during the storage period as well as the total antioxidant capacity, using the method of Cao et al. (Clin. Chem. 1995, 41/12 1739 1744). There were significant differences in all parameters among the species, and after storage. Across all species and storage treatments, the antioxidant capacity was correlated with the content of phenolics (R = 0.77) and anthocyanins (R = 0.85). Both species of blueberry had more than twice the antioxidant capacity of either raspberry or strawberry; antioxidant capacity of the highbush and low-bush blueberries did not change during storage. During storage at temperatures greater than 0 °C, anthocyanin content of raspberries and strawberries increased substantially, and this increase was accompanied by an increase in total antioxidant capacity. Since dietary antioxidants such as anthocyanin flavonoids have been suggested to have positive health benefits, postharvest anthocyanin formation may have a desirable effect on the health quality of certain fruit.

2021 ◽  
Vol 48 (No. 4) ◽  
pp. 183-189
Author(s):  
Aruma B.H.K. De Silva ◽  
H.P. Vasantha Rupasinghe

The haskap has been successfully cultivated in many geographical zones in Canada. We investigated whether the latitude has any impact on the anthocyanin accumulation and total antioxidant capacity in the haskap berry. The measured quality attributes were compared with that of the blueberry. Haskap ‘Aurora’ berries were collected from Yukon, British Columbia, Saskatchewan, and Nova Scotia in Canada, and blueberries were collected from Nova Scotia. Cyanidin-3-O-glucoside (C3G), the most abundant anthocyanin of haskap berry, the total anthocyanins, the total polyphenols, the soluble solids (°Brix), the pH value, the total antioxidant capacity, and the moisture content were determined. The results revealed that the total anthocyanin, total phenolic content, and antioxidant capacity of the haskap range between 88 to 273 mg C3GE/100 g fresh weight (FW), 256 to 442 mg GAE/100 g of FW, and 27 to 52 µmol TE/g FW, respectively. The liquid chromatography-mass spectrometry (UPLC/MS) analysis revealed that the C3G contained in the haskap berry is highly dependent on the harvest stage. Among the samples, the haskap berry harvested on July 19, 2019, from the Yukon had the highest C3G content. Interestingly, the total anthocyanin content of the haskap berry is comparable to that of the lowbush blueberry, but higher than the highbush blueberry. A systematic multi-year experiment employing many cultivars and growing locations is recommended to better understand the latitude effect.


2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
C. Priyanthi ◽  
R. Sivakanesan

Background. The present study evaluates the antioxidant properties of some Sri Lankan red rice varieties using water extracts. Methods. Water extracts of rice varieties Attakkari, Bg2907, and Bg407 were used in this study. The total antioxidant capacity was measured by ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and reducing power assays. The total phenolic content (TPC), total flavonoid content (TFC), monomeric anthocyanin, and condensed tannin contents were measured by Folin-Ciocalteu, aluminium chloride, pH differential, and vanillin assays, respectively. Results. It was observed that mean FRAP, DPPH, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content were in the range of 0.561 ± 0.113 to 0.695 ± 0.077   mmol / 100   g fresh weight (FW), 26.07 ± 3.08 to 53.66 ± 7.61   mg / mL   FW , 33.49 ± 4.105.14 to 40.81 ± 3.65   mg / mL , 0.676 ± 0.078 to 0.900 ± 0.057   mg tannic acid equivalent (TAE)/g, 5.36 ± 0.75 to 6.38 ± 0.82   mg   TAE / g   FW , 0.0202 ± 0.005 to 0.0292 ± 0.009   mg / g   FW , and 0.078 ± 0.015 to 0.104 ± 0.017   mg   TAE / g   FW , respectively. Significant differences were observed in DPPH, reducing power, and TPC among rice varieties ( p < 0.05 ). Rice variety Attakkari had the highest total antioxidant capacity (TAC), scavenging activity, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content followed by Bg2907 and Bg406. Conclusion. Total phenolic compounds, total flavonoid, and condensed tannin are the major antioxidants in all three varieties of rice while the monomeric anthocyanin is only a minor antioxidant.


2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel


2013 ◽  
Vol 2 (2) ◽  
pp. 33-37 ◽  
Author(s):  
Fatema Nasrin

Modern civilization is facing more than hundreds of disorders associated with free radicals and natural antioxidants from non-edible plants are gaining importance to fight these disorders. The intention of this report is to evaluate a well known medicinal weed Ageratum conyzoides stems for its antioxidant and cytotoxic Effects. Antioxidant potentiality of the crude methanolic extract of the Ageratum conyzoides (AC) stems was investigated on DPPH scavenging activity, reducing ability, total antioxidant capacity as well as total phenolic contents. Cytotoxic study was done by brine shrimp lethality bioassay and vincristin sulphate was used as standard. The total phenols and total antioxidant capacity of AC was found to be 38.125 ± 2.01mg/g equivalent of gallic acid and 333.37 ± 4.22mg/gm equivalent of ascorbic acid, respectively. The percentage (%) scavenging of DPPH free radical of the extract was found to be concentration dependent with IC50 value 46.01 ± 2.23µg/ml while IC50 value of standard ascorbic acid was found to be 29.56 ± 0.11?g/ml. The reducing power of AC was found to be concentration dependent. The cytotoxicity exhibited by AC was found promising with LC50 value 1.32?g/ml, comparing with the LC50 (0.689?g/ml) values of vincristin sulphate. The present investigation suggests that Ageratum conyzoides possesses remarkable antioxidant and cytotoxic property.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13195 International Current Pharmaceutical Journal 2013, 2(2): 33-37


2017 ◽  
Vol 7 (1) ◽  
pp. 12-16

ABSTRACT Aim To assess and correlate the level of stress to the total antioxidant capacity of saliva of university exam-going students studying at KLE Vishwanath Katti Institute of Dental Sciences, Belagavi, Karnataka, India, on the day of examination (stressed condition) and in the postexamination period (nonstressed condition). Materials and Methods Saliva samples of 30 students evaluated by the perceived stress scale on both the day of examination and in the postexamination period were collected and labeled as group I (day of examination) and group II (postexamination period). The total antioxidant capacity was assessed using the spectrophotometric assay based on the molar absorption coefficient of the phosphomolybdenum complex. The correlation between the level of stress and the antioxidant capacity was done using Spearman's correlation test, and the mean values obtained with respect to the two groups were evaluated using Mann–Whitney U test. Results The total antioxidant capacity was lower on the day of examination than in the postexamination period (p = 0.001). As the stress level increases, the total antioxidant capacity decreases. Conclusion Total antioxidant capacity of saliva can serve as an effective marker of stress. Thus, supplementation with antioxidants routinely and especially during periods of stress may be beneficial. Clinical significance Present day life is full of targets, necessities, and frustrations. For many people, stress has become a way of life. Stress is not always detrimental. Stress within your comfort zone helps you compete, motivates you, and can even keep you secure. But when stress becomes overwhelming, it damages your well-being, relationships, and quality of life. Thus, managing and diagnosing stress is important. How to cite this article de Piedade Sequeira MAKE, Naik Z. Total Antioxidant Capacity of Saliva: An Effective Marker of Stress. J Contemp Dent 2017;7(1):12-16.


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Zakaria Khiya ◽  
Yassine Oualcadi ◽  
Abderrahmane Gamar ◽  
Fatima Berrekhis ◽  
Touria Zair ◽  
...  

The purpose of this study is to determine the total content of phenols, flavonoids, and condensed tannins, as well as on the antioxidant activity of the extract, and their fractions were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), phosphomolybdate reduction (or total antioxidant capacity), and cyclic voltammetry (CV). The hydromethanolic extract of Salvia officinalis showed the highest values of total phenolic (176 mgGAE/g of extract) and condensed tannins (162.53 mgEC/g of extract) from the Boulemane and Khenifra regions, respectively. The results showed that the best DPPH assay was found in the ethyl acetate fraction of Salvia officinalis leaves of the Boulemane region (IC50 = 0.002 mg/ml). For the ethyl acetate and butanolic fractions of Salvia officinalis leaves, those collected from different regions have a better reducing capacity (EC50 = 0.021 mg/ml, respectively). For the total antioxidant capacity, the best activity was found in the aqueous fraction of Salvia officinalis leaves of the Boulemane region (108 mgGAE/g of extract). By the cyclic voltammetry method, hydromethanolic extract of Salvia officinalis leaves from the Boulemane region showed an important result (288.8 mgGAE/g). There was a positive correlation between total phenol content (TPC), condensed tannin content (TCT), and total antioxidant capacity (TAC) (r = 0.932, r = 0.896, respectively). The main compounds that have been identified in the hydromethanolic extract of Salvia officinalis are ascorbic acid, gallic acid, 4-hydroxybenzoic acid, tannic acid, and rutin. Due to their antioxidant property, the leaf extracts from Salvia officinalis are used as natural preservative ingredients in food and/or pharmaceutical industries.


2021 ◽  
Vol 7 (10) ◽  
pp. 851
Author(s):  
Ezelhan Selem ◽  
Yekbun Alp ◽  
Suat Sensoy ◽  
Yusuf Uzun ◽  
Seyda Cavusoglu ◽  
...  

Recently, the production of macro-fungi (mushrooms) has steadily increased, and so has their economic value, in global terms. The use of functional foods, dietary supplements, and traditional medicines derived from macro-fungi is increasing as they have numerous health benefits as well as abundant nutrients. This study aimed to determine some biochemical contents (pH, soluble solid contents (SSC), total antioxidant capacity (TAC) and total phenolic contents (TPC)) of eight edible macro-fungi species growing naturally (in the wild) in Turkey. The samples were collected in the Van Yuzuncu Yil University (VAN YYU) campus area in the months of April–May 2018, in different locations, and brought to the laboratory, and the necessary mycological techniques were applied for their identification. Location, habitats, collection dates and some morphological measurements were determined for all identified species. Biochemical parameters of the macro-fungi species were analyzed separately both in cap and stem. The color values (L, a, b, Chroma and hue) were separately evaluated on cap surface, cap basement and stem. Results showed that there were significant differences for most of the biochemical parameters in different organs between and within species. The pH, SSC, TAC and TPC values varied from 6.62 to 8.75, 2.25 to 5.80° brix, 15.72 to 57.67 TE mg−1 and 13.85 to 60.16 gallic acid equivalent (GAE) fresh weight basis. As a result of the study, it was concluded that the parameters such as total antioxidant capacity, total phenolic content and soluble content in Morchella esculenta, Helvella leucopus, Agaricus bitorquis and Suillus collinitus were higher than for the other species and clearly implied that they may be further exploited as functional ingredients in the composition of innovative food products.


Author(s):  
R. Altintas ◽  
O. Kankavi

Colostrum is the primary source of initial acquired immunity and provides an antioxidative protection of newborn. The aim of present study was to analyze and compare the changes in total antioxidant capacity (TAC) and total phenolic contents (TPCs) colostrum’s of Honamli and Hair goat breeds on the first 5 days after parturition. The spectrophotometric ABTS method and the Folin–Ciocalteu assay were used. TAC and TPCs values were decreased gradually as the transition period advances. Both values were highest on the day first of calving as compared to rest of the days. Honamli goat colostrum samples had a higher TAC value than Hair goat and was showed significant (P less than 0.05) difference amongst the breeds. However, TPCs in Hair goat colostrum showed highly significant (P less than 0.05) difference than those of Honamli goat. In conclusion, TAC and TPC values in goat colostrum showed differences among goat breeds and exhibits daily rational changes. 


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