scholarly journals Total Antioxidant Capacity of Saliva: An Effective Marker of Stress

2017 ◽  
Vol 7 (1) ◽  
pp. 12-16
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Life Stress ◽  
Well Being ◽  
Stressed Condition ◽  
Way Of Life ◽  
Mean Values ◽  
Group I ◽  
Total Antioxidant

ABSTRACT Aim To assess and correlate the level of stress to the total antioxidant capacity of saliva of university exam-going students studying at KLE Vishwanath Katti Institute of Dental Sciences, Belagavi, Karnataka, India, on the day of examination (stressed condition) and in the postexamination period (nonstressed condition). Materials and Methods Saliva samples of 30 students evaluated by the perceived stress scale on both the day of examination and in the postexamination period were collected and labeled as group I (day of examination) and group II (postexamination period). The total antioxidant capacity was assessed using the spectrophotometric assay based on the molar absorption coefficient of the phosphomolybdenum complex. The correlation between the level of stress and the antioxidant capacity was done using Spearman's correlation test, and the mean values obtained with respect to the two groups were evaluated using Mann–Whitney U test. Results The total antioxidant capacity was lower on the day of examination than in the postexamination period (p = 0.001). As the stress level increases, the total antioxidant capacity decreases. Conclusion Total antioxidant capacity of saliva can serve as an effective marker of stress. Thus, supplementation with antioxidants routinely and especially during periods of stress may be beneficial. Clinical significance Present day life is full of targets, necessities, and frustrations. For many people, stress has become a way of life. Stress is not always detrimental. Stress within your comfort zone helps you compete, motivates you, and can even keep you secure. But when stress becomes overwhelming, it damages your well-being, relationships, and quality of life. Thus, managing and diagnosing stress is important. How to cite this article de Piedade Sequeira MAKE, Naik Z. Total Antioxidant Capacity of Saliva: An Effective Marker of Stress. J Contemp Dent 2017;7(1):12-16.

scholarly journals Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

2017 ◽  
Vol 45 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Andrei CONEAC ◽  
Meda Sandra ORASAN ◽  
Daniel Corneliu LEUCUTA ◽  
Nicoleta DECEA ◽  
Miuta FILIP ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Experimental Inflammation ◽  
Group Ii

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

Changes in Fruit Phenolic Composition and Antioxidant Capacity during Storage

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 469b-469 ◽  
Author(s):  
W. Kalt ◽  
C.F. Forney ◽  
J. McDonald
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Storage Period ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Positive Health ◽  
Total Antioxidant ◽  
Health Quality ◽  
And Storage

Ripe fruit of strawberry, raspberry, and highbush and lowbush blueberries were stored at 0, 10, 20, or 30 °C for up to 8 days. Total phenolic and anthocyanin content was measured at regular intervals during the storage period as well as the total antioxidant capacity, using the method of Cao et al. (Clin. Chem. 1995, 41/12 1739 1744). There were significant differences in all parameters among the species, and after storage. Across all species and storage treatments, the antioxidant capacity was correlated with the content of phenolics (R = 0.77) and anthocyanins (R = 0.85). Both species of blueberry had more than twice the antioxidant capacity of either raspberry or strawberry; antioxidant capacity of the highbush and low-bush blueberries did not change during storage. During storage at temperatures greater than 0 °C, anthocyanin content of raspberries and strawberries increased substantially, and this increase was accompanied by an increase in total antioxidant capacity. Since dietary antioxidants such as anthocyanin flavonoids have been suggested to have positive health benefits, postharvest anthocyanin formation may have a desirable effect on the health quality of certain fruit.

scholarly journals Influence of crop load on the yield and grape quality of Merlot and Vranac (Vitis vinifera L.) varieties in Trebinje vineyard

2021 ◽  
Vol 117 (4) ◽  
pp. 1
Author(s):  
Mersija DELIĆ ◽  
Fikreta BEHMEN ◽  
Saša MATIJAŠEVIĆ ◽  
Šaćira MANDAL ◽  
Saud HAMIDOVIĆ ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Phenolics ◽  
Soluble Solids ◽  
Crop Load ◽  
Grape Quality ◽  
Total Antioxidant ◽  
The Impact

<p class="042abstractstekst">The aim of this study was to study the impact of crop load on the yield and grape quality of ‘Merlot’ and ‘Vranac’ (<em>Vitis vinifera</em> L.) in Trebinje vineyard. The crop load levels studied in this trial were 9 buds (V1) and 12 buds (V2) per vine at each variety trained on Lenz-Moser bilateral cordon system. The impact was determined by measurements of yield per vine and grape quality characterized by the contents of total soluble solids, titratable acidity, total phenolics, total flavonoids and by total antioxidant capacity. The measured parameters of grape quality of ‘Merlot’ and ‘Vranac’ were not influenced significantly by crop load levels. V2, compared to the V1, showed the potential for increasing grape yield only for Merlot variety under experimental conditions. The results of this study also showed a positive correlation between total phenolics/flavonoids and total antioxidant capacity of grape berries in both varieties, regardless of crop loads applied.</p>

Increased total antioxidant capacity in renal tissue of female BWF1 mice infected with Plasmodium chabaudi

2017 ◽  
Author(s):  
Mostafa A. Abdel- Maksoud ◽  
Fathy A. Abdel- Ghaffar ◽  
Azza El- Amir ◽  
Gamal Badr ◽  
Saleh Al- Quraishy
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Renal Tissue ◽  
Hepatic Tissue ◽  
Control Group ◽  
Plasmodium Chabaudi ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Urine Biochemistry

We have investigated the effect of malaria infection on the urine biochemistry and the renal and hepatic levels of total antioxidant capacity (TAC) in female BWF1 lupus mice. A total of 30 female BWF1 lupus mice were divided into three groups as follows: group (I) control group; group (II) lupus mice infected with live Plasmodium. chabaudi; group (III) lupus mice infected with irradiated P. chabaudi. Mice were killed at day 14 post-infection and plasma samples were collected. live P. chabaudi infection was associated with decreased level of glucose in urine (glucosuria), increased urinary level of both ketones (ketonuria) and blood (hematuria). Additionally, live P. chabaudi infection was associated with increased level of TAC in the renal tissue with decreased level of TAC in the hepatic tissue of infected mice. In conclusion, P. chabaudi infection has a direct effect on the urine biochemistry and the renal and hepatic levels of TAC in BWF1 mice.

scholarly journals Comparative Evaluation and Correlation of Salivary Total Antioxidant Capacity and Salivary pH in Caries-free and Severe Early Childhood Caries Children

2015 ◽  
Vol 16 (3) ◽  
pp. 234-237 ◽  
Author(s):  
Meenakshi Nankar ◽  
Hrishikesh Walimbe ◽  
Mohammed Nadeem Ahmed Bijle ◽  
Sneha Muchandi ◽  
Srishti Chaturvedi ◽  
...  
Keyword(s):  
Early Childhood ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Comparative Evaluation ◽  
Early Childhood Caries ◽  
Group I ◽  
Salivary Ph ◽  
Total Antioxidant ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

ABSTRACT Background Dental caries is a major problem in preschool children. The contribution of saliva in providing defense during caries process is of primary importance. pH buffer capacity through bicarbonate, phosphate and protein buffer systems have universal acceptance as a caries defense mechanism. Antioxidant capacity of saliva can constitute a first line of defense against chronic degenerative diseases including dental caries. Till date, no study is presented with salivary antioxidant capacity of younger children affected with severe early childhood caries with its salivary pH correlation. Hence, this study was carried out to compare, evaluate and correlate the salivary total antioxidant capacity (TAC) and salivary pH of children with caries-free and severe early childhood caries. Materials and methods Fifty children from ages 3 to 5 years divided into two study groups had undergone screening. Group I (n = 25) with severe early childhood caries (S-ECC) and group II (n = 25) who were caries free. Unstimulated whole saliva of subjects were in the collection during the study by draining method. Salivary pH determination of saliva samples was done using pH indicator paper strips. The TAC was done using an antioxidant assay with the help of a spectrophotometer at wavelength 532 nm. The means of salivary pH and TAC were subjected to analysis using unpaired student ‘t’ test and correlation was determined using Pearsons correlation coefficient analysis. Results Mean salivary pH was higher in group II (7.46 ± 0.37). Mean TAC was greater in group I (1.82 ± 0.19). A statistically significant negative correlation as seen between TAC and salivary pH in S-ECC patients. Conclusion The study concludes that salivary TAC increases in patients with S-ECC are by that showing a high indirect relationship with salivary pH. How to cite this article Muchandi S, Walimbe H, Bijle MNA, Nankar M, Chaturvedi S, Karekar P. Comparative Evaluation and Correlation of Salivary Total Antioxidant Capacity and Salivary pH in Caries-free and Severe Early Childhood Caries Children. J Contemp Dent Pract 2015;16(3):234-237.

107 Administration of antioxidants improves serum total antioxidant capacity, testosterone, and seminal quality of Magra rams

2021 ◽  
Vol 33 (2) ◽  
pp. 160
Author(s):  
A. Kumar ◽  
J. S. Mehta ◽  
A. Kumar ◽  
G. N. Purohit
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Semen Parameters ◽  
Total Antioxidant

Oxidative stress is one of the most important factors responsible for poor quality of semen, and it is defined as an imbalance between the formation of reactive oxygen species (ROS, including hydroxyl radicals, superoxide anions, and hydrogen peroxide) and antioxidant capacity of semen. Antioxidants act as a powerful defence system against ROS attack in sperm cells by decreasing oxidative stress and they improve semen quality, which could be useful in the management of male infertility. Magra is a dual-purpose (wool and meat) hardy sheep breed that originated in the eastern and southern parts of the Bikaner district of Rajasthan, India. The present study evaluated the effects of the administration of vitamin E with selenium or vitamin C on serum antioxidants, testosterone, and seminal quality of Magra rams. Twenty-one Magra rams, aged between 1.5 and 2.5 years, were randomly assigned to treatment at weekly intervals with administration of either 1mL of vitamin E (tocopherol, 50mg mL−1) and selenium (1.5mg sodium selenite; n=7; VES), 8mL of vitamin C (250mg mL−1 sodium ascorbate; n=7; VC), or 1mL of saline (n=7; control) subcutaneously for 5 consecutive weeks. After the fourth week, semen and blood were collected twice a week for 5 consecutive weeks and assayed for seminal quality parameters, serum testosterone, and serum antioxidants. Data obtained were analysed statistically using te SPSS software (version 25.0; IBM Corp.). Mean values were compared using Duncan’s multiple range test. The semen parameters sperm motility, sperm concentration, live sperm percentage, and% HOST (hypo-osmotic swelling test)-positive sperm were significantly elevated, whereas reaction time and percentage of abnormal sperm were lower in both the treated groups compared with controls (P&lt;0.05). Seminal pH declined significantly in the VES group (P&lt;0.05), whereas it was not different between the VC and control groups of rams (P&gt;0.05). Glutathione peroxidase, superoxide dismutase, and total antioxidant capacity concentrations were higher in serum and seminal plasma of treated rams (P&lt;0.05). Serum testosterone concentrations were significantly elevated in VES and VC groups (P&lt;0.05). We concluded that the administration of vitamin E and selenium or vitamin C improves the serum testosterone production and seminal quality of rams.

scholarly journals Hygienic-sanitary quality and effect of freezing time and temperature on total antioxidant capacity of human milk

2020 ◽  
Vol 23 ◽  
Author(s):  
Lilian Maria Peixoto Lopes ◽  
Jaísa Oliveira Chaves ◽  
Luciana Rodrigues da Cunha ◽  
Maria Cristina Passos ◽  
Camila Carvalho Menezes
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Storage Temperature ◽  
Freezing Temperature ◽  
Storage Conditions ◽  
Freezing Time ◽  
Total Antioxidant ◽  
Functional Components

Abstract Knowledge of the hygienic-sanitary quality of raw human milk helps to establish the best storage conditions for the preservation of its nutritional and functional components. Therefore, this study aimed to evaluate the hygienic-sanitary quality of human milk extracted at the mothers’ homes, and to analyze the effect of freezing time and temperature on the milk’s total antioxidant capacity. Milk of five mothers was evaluated individually for Staphylococcus aureus, total aerobic mesophilic counts, mold and yeasts, total and thermotolerant coliforms. The milk pool was stored at -8 °C, -18 °C and -40 °C for 2, 4, 8 and 15 days and analyzed for the total antioxidant capacity by the 2,2-diphenyl-1-picrylhydrazyl free radical and 2,2-azino 3-ethylbenzothiazoline-6-sulfonic acid scavenging methods. The microbiological analyses of the raw human milk samples indicated counts of all the studied microorganisms above the safe limits. Related to the effect of freezing time and temperature on total antioxidant capacity, it was concluded that, regardless of the freezing temperature, a significant reduction occurred on total antioxidant capacity over time and that the lower the storage temperature, the greater its total antioxidant activity.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Summer Day/Night Gender Differences in Serum Total Antioxidant Capacity as a Methodological Pitfall in Human Antioxidant Research

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Armando L. Morera-Fumero ◽  
Pedro Abreu-Gonzalez ◽  
Lourdes Fernandez- Lopez ◽  
Estefanía Díaz-Mesa ◽  
Maria del Rosario Cejas-Mendez
Keyword(s):  
Gender Differences ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant
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