scholarly journals Seed Ontogeny and Nonstructural Carbohydrates of Cycas micronesica Megagametophyte Tissue

HortScience ◽  
2016 ◽  
Vol 51 (9) ◽  
pp. 1144-1147 ◽  
Author(s):  
Thomas E. Marler ◽  
Nirmala Dongol

The profile of nonstructural carbohydrates (NSC) was quantified to determine sugar and starch relationships of megagametophyte tissue during Cycas micronesica K.D. Hill seed ontogeny. Field work occurred in northern Guam where megastrobili were marked and dated as they emerged from stem apices of plants in a natural population. Seeds were harvested beginning 6 months after megastrobili emergence and continuing until 28 months, and gametophyte tissue was separated from the remainder of each seed. Carbohydrates within lyophilized gametophyte tissue were quantified by high-pressure liquid chromatography. The levels of glucose and fructose declined from a high at 6 months to a homeostasis at 11 months, and the levels of sucrose similarly declined from 6 months to a homeostasis at ≈14 months. Starch content exceeded sugar content and increased from 6 months to reach a homeostasis at ≈18 months. Maltose was not detected in any sample. Stoichiometric quotients changed dramatically until ≈14 months, when they became fairly stable until 28 months. Starch concentration was ≈5-fold greater than sugar concentration at 6 months, and increased to ≈15-fold greater than sugar concentration by 28 months. Total NSC in mature megagametophytes was almost 70% on a dry weight basis. Our results are in agreement with the biological function of this haploid tissue, as copious carbohydrate resources are readily available to support embryo and seedling growth.

2021 ◽  
Vol 108 (september) ◽  
Author(s):  
Varshini S V ◽  
◽  
Jayanthi C ◽  

A field experiment was conducted at the Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, to study the influence of sett treatment on biochemical parameters, weed parameters, physiological parameters and nutrient uptake of bajra Napier hybrid grass. The results of the experiment indicated that on biochemical parameters, sett treatment with water (12 hours soaking fb 24 hours incubation) (S1) recorded higher reducing sugars (28.89 mg/g), total sugar (34.83 mg/g) and starch content (45.83 mg/g). Whereas the lower non reducing sugar content (5.94 mg/g) and total phenolics content (1.65 mg/g) were also observed with sett treatment with water (12 hours soaking fb 24 hours incubation) (S1). Similarly, on weed parameters, significantly lower weed density (9.90 No./m2) and weed dry weight (12.92 g/m2) was recorded with sett treatment with water (12 hours soaking fb 24 hours incubation) (S1). On physiological parameters, significantly higher leaf area index (28.98), relative water content (87.85 %) was registered with water (12 hours soaking fb24 hours incubation) (S1). Among sett treatments, water (12 hours soaking fb24 hours incubation) (S1) had registered higher nitrogen (192.8 kg ha-1), phosphorus (33.0 kg ha-1) and potassium (112.9 kg ha-1) uptake.


1978 ◽  
Vol 58 (1) ◽  
pp. 199-206 ◽  
Author(s):  
M. TOLLENAAR ◽  
T. B. DAYNARD

Kernel development was studied in the maize (Zea mays L.) hybrids United-H106 and Funk’s G-4444, grown in a controlled-environment growth room. A method was employed in which husks were excised, and kernels were removed from the same set of ears at several subsequent sampling dates. This method did not affect the dry matter accumulation of the remaining kernels. Basal kernels (kernel numbers 6–15 in the row) and tip kernels (kernel numbers 31–40) were removed at 2-day intervals during the period from 10 to 20 days postsilking. Dry weight, ethanol-soluble sugar content, and starch content were determined for each sample. Accumulation of dry matter in the tip kernels ceased in a fraction of the United-H106 ears at the onset of the period of linear tip-kernel dry matter accumulation. Only small differences were observed in sugar content between growing and non-growing tip kernels of ears of United-H106. Starch appeared to continue to accumulate in kernels in which dry matter had ceased to accumulate. Except for a delay of approximately 2 days, the pattern of development of tip kernels in Funk’s G-4444 was similar to that of kernels at the base.


1990 ◽  
Vol 70 (2) ◽  
pp. 455-460 ◽  
Author(s):  
C. CHATTERJEE ◽  
P. SINHA ◽  
S. C. AGARWALA

Boron-phosphorus interaction was observed in maize (Zea mays L. ’Ganga 2’) when grown in refined sand at three levels of boron, deficient (0.0033 mg L−1), normal (0.33 mg L−1) and excess (3.3 mg L−1), each at three levels of phosphorus, deficient (0.17 m mol L−1), normal (1.5 mmol L−1) and excess (4 m mol L−1). The effects of phosphorus deficiency (i.e., reduction in dry matter, soluble protein, DNA, activity of ribonuclease and increase in the activities of peroxidase, acid phosphatase and polyphenol oxidase) were intensified by a combined deficiency of boron and phosphorus. The effects of boron deficiency (i.e., reduction in dry weight, leaf boron and DNA and increase in starch content and in the activities of starch phosphorylase, peroxidase and polyphenol oxidase) become more intense in the treatment-deficient B-excess P. The decreases caused by excess phosphorus (i.e., dry weight, starch and sugar content, DNA, RNA and activity of ribonuclease) were aggravated by combined excess of boron and phosphorus.Key words: Zea mays, maize, boron and phosphorus nutrition


2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Phanat Kittiphattanabawon ◽  
Sitthipong Nalinanon ◽  
Soottawat Benjakul ◽  
Hideki Kishimura

Pepsin-solubilised collagen from the skin of splendid squid (SC) was isolated, partially purified by salt precipitation and dialysis prior to characterisation. The yield of SC was 75.3% (dry weight basis). SC with high purity was obtained as shown by the distinct UV absorption peak at 232 nm and high hydroxyproline content. Total sugar content of SC was 4.70% (dry weight basis), which was higher than that of collagen from calf skin (CC) (1.45% dry weight basis) (P<0.05). Based on SDS-PAGE and elution profile, SC might contain the mixed types of collagen (type SQ-I and type SQ-II), in whichα- andβ-chains were the major components. SC was rich in glycine and had high content of imino acids (189 residues/1000 residues). The degradation induced by chymotrypsin and lysyl endopeptidase was more pronounced in CC, compared with SC. The maximum transition temperature (Tmax) of SC was 34.1°C, which was about 7°C lower than that of CC. Fourier transform infrared spectra revealed that the triple-helical structure of SC was predominant with the copresence of carbohydrate moieties. Therefore, the skin of splendid squid, a byproduct from squid processing, can be an alternative source for collagen production.


2021 ◽  
Vol 8 (2) ◽  
pp. 283-288
Author(s):  
Tasneem Rangwala ◽  
Angurbala Bafna ◽  
Nagesh Vyas ◽  
Rohan Gupta

Fertilisers have become an important factor used by farmers to increase yield and improve product quality. Earth’s crust carries a large amount of elemental silicon. However, silicon is not considered an essential element for plant growth therefore is not included in fertilisers. Silicon has shown to enhance the growth and productivity of various crops. The present study aimed to explore the potential of soluble silica in improving the biochemical parameters of banana (Grand naine variety). The field experiment was conducted at Ropni Vasaad village, Burhanpur District of Madhya Pradesh from August 2017 to September 2018. Silica was supplied as potassium silicate in the liquid form under the trade name AgriboosterTM. Doses were administered at the interval of one month starting from planting the tissue culture explants till harvesting the final crop. Eight treatments were designed which included three different concentration of soluble silica applied alone and with combination with compound fertilisers. Control was without any treatment. A significant increase in fresh and dry weight was observed with all the treatments. All the combinations of soluble silica resulted in significant increase in starch and total sugar content. The protein content showed significant increase with treatments consisting of soluble silica and compound fertiliser. Cellulase and amylase activity declined on treatment with soluble silica. The present study reveals that if soluble silica is either applied alone or with compound fertiliser, it can enhance the biochemical parameters and can indirectly delay ripening of banana by altering activity of cellulase and amylase.


Author(s):  
Vishwanath Bidaramali ◽  
Shirin Akhtar ◽  
Anupam Das

Eggplant (Solanum melongena L.) is an important solanaceous crop of tropics and sub-tropics, having high production potential, and also a rich source of nutrients, particularly, carbohydrates, proteins, dietary fibre and vitamins like thiamin, niacin, pantothenic acid and folic acid, minerals like calcium, iron, potash, zinc, copper and manganese as well as bioactive compounds. However, eggplant is available in diverse shape and colour and consumer preference varies and the nutrient composition is different. In this investigation, we worked out the proximate nutrient compounds (on dry weight basis) and the bioactive compounds (on a fresh weight basis) in twenty diverse eggplant genotypes varying in colour and shape.  Moisture content in the fruits ranged from 71.54 to 91.36%, while carbohydrate content from 2.80 to 6.82%, crude protein 16.98 to 31.85%, nitrogen 2.49 to 4.35%, phosphorous 0.29 to 0.51%, potassium 1.65 to 4.54%, calcium 0.83 to 0.35%, iron 106.21 to 235.34 mg/kg, manganese 89.01 to 245.54 mg/kg, copper 18.73 to 98.56 mg/kg and zinc 60.73 to 75.77 mg/kg, each on dry weight basis. Biochemical parameters like total soluble solids (TSS) ranged from 1.27 to 3.94 °Brix, total sugar content 2.26 to 4.65%, ascorbic acid 0.66 to 3.53 mg/100 g, total anthocyanin content 0.35 to 18.85 mg/100 g FW, total chlorophyll 0.11 to 2.70 mg/100 g FW, total phenol 1.03 to 15.65 mg catechol equivalent/100 g FW, total antioxidant 1.16 to 2.26 µmol Trolox equivalent/g FW and radical scavenging activity 50.52 to 96.48%. Proximate nutrients were highest in Pusa Uttam, Pant Rituraj and BRBL-01, quality parameters in BRBL-07 and 71-19, while bioactive compounds were highest in Pant Rituraj, Pusa Purple Long, Pusa Purple Cluster and BRBL-01. These genotypes may be utilized in future breeding programmes for developing effective and nutritive cultivars.


1973 ◽  
Vol 51 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Gaurangakumar Das

Effects of gibberellin A3 (GA) on growth, pigments, protein, and nucleic acid content of the cotyledons of lettuce seedlings (Lactuca sativa L. cv. Grand Rapids) are reported. GA treatment increased both the total shoot and cotyledon fresh weights, and decreased chlorophyll content per cotyledon and on a dry weight basis. GA also decreased the protein content of the cotyledon, but increased the level of RNA and DNA measured on a dry weight basis. The hormone had no effect on the pheophytin: chlorophyll ratio, but increased the ratios of chlorophyll a: chlorophyll b, phytol: chlorophyll, and carotene: chlorophyll. GA also increased the sugar content of the cotyledons. Treatment of excised cotyledons with glucose had an effect similar to that of GA on chlorophyll and protein content. The data are discussed in relation to the mechanism of GA-induced chlorosis.


1977 ◽  
Vol 89 (3) ◽  
pp. 589-597 ◽  
Author(s):  
A. B. McAllan ◽  
R. H. Phipps

SummaryThe (early maturing) maize hybrid, Anjou 210, was sown on 1 May (1975) and 50000 (LD) and 150000 (HD) plants/ha established. Leaf, stem, ear and husk components of the shoots, and whole shoots were separately examined for changes in dry weight, soluble sugars, starch, hemicellulose and cellulose at regular intervals from 104 to 154 days after planting together with whole shoots after ensiling.Throughout the sampling period whole shoot and ear dry weights increased steadily whereas those of leaf and stem decreased in plants from both treatments.Total soluble sugar contents for plants grown at LD were at a maximum 104 days after planting compared with 125 days after planting for the HD crop. Immediately prior to ensiling whole shoot soluble sugar content was 74 and 93 g/kg D.M. for LD and HD treatments respectively.Small amounts of starch (g/kg D.M.) were found in stems (10–25), husks (40–80) and leaves (17–35) of plants grown at both densities. The starch content of the ear increased from 57 to 390 g/kg D.M. and 168 to 376 g/kg D.M. in plants grown at LD and HD respectively.For plants from both treatments hemicellulose contents of husks, leaves and whole shoots increased with age whereas those of stems decreased. Cellulose contents did not vary greatly over the growing season.On ensiling plants from both densities, total soluble sugars had virtually disappeared after only 3 days. Apparent losses (approximately 0·3 g/g), over a longer period of time, were observed in hemicellulose sugars and starch. Apparent increases were observed in cellulose contents of approximately 0·15 g/g.


Author(s):  
I.M. Ritchie ◽  
C.C. Boswell ◽  
A.M. Badland

HERBACE DISSECTION is the process in which samples of herbage cut from trials are separated by hand into component species. Heavy reliance is placed on herbage dissection as an analytical tool ,in New Zealand, and in the four botanical analysis laboratories in the Research Division of the Ministry of Agriculture and Fisheries about 20 000 samples are analysed each year. In the laboratory a representative subsample is taken by a rigorous quartering procedure until approximately 400 pieces of herbage remain. Each leaf fragment is then identified to species level or groups of these as appropriate. The fractions are then dried and the composition calculated on a percentage dry weight basis. The accuracy of the analyses of these laboratories has been monitored by a system of interchanging herbage dissection samples between them. From this, the need to separate subsampling errors from problems of plant identification was, appreciated and some of this work is described here.


2018 ◽  
Vol 18 (1) ◽  
pp. 10-15
Author(s):  
Wang Yi-Wei ◽  
He Yong-Zhao ◽  
An Feng-Ping ◽  
Huang Qun ◽  
Zeng Feng ◽  
...  

In this study, Chinese yam starch-water suspension (8%) were subjected to high-pressure homogenization (HPH) at 100 MPa for increasing cycle numbers, and its effect of on the physicochemical properties of the starch was investigated. Results of the polarizing microscope observations showed that the starch granules were disrupted (i.e. greater breakdown value) after HPH treatment, followed by a decrease in cross polarization. After three HPH cycles, the crystallinity of starch decreased, while the crystal type remained unaltered. Meanwhile, the contents of rapidly digestible starch and slowly digestible starch were increased. On the contrary, resistant starch content was decreased. Our results indicate that HPH treatment resulted in reduction of starch crystallinity and increase of starch digestibility.


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