The effect of sample date and plant density on the carbohydrate content of forage maize and the changes that occur on ensiling

1977 ◽  
Vol 89 (3) ◽  
pp. 589-597 ◽  
Author(s):  
A. B. McAllan ◽  
R. H. Phipps

SummaryThe (early maturing) maize hybrid, Anjou 210, was sown on 1 May (1975) and 50000 (LD) and 150000 (HD) plants/ha established. Leaf, stem, ear and husk components of the shoots, and whole shoots were separately examined for changes in dry weight, soluble sugars, starch, hemicellulose and cellulose at regular intervals from 104 to 154 days after planting together with whole shoots after ensiling.Throughout the sampling period whole shoot and ear dry weights increased steadily whereas those of leaf and stem decreased in plants from both treatments.Total soluble sugar contents for plants grown at LD were at a maximum 104 days after planting compared with 125 days after planting for the HD crop. Immediately prior to ensiling whole shoot soluble sugar content was 74 and 93 g/kg D.M. for LD and HD treatments respectively.Small amounts of starch (g/kg D.M.) were found in stems (10–25), husks (40–80) and leaves (17–35) of plants grown at both densities. The starch content of the ear increased from 57 to 390 g/kg D.M. and 168 to 376 g/kg D.M. in plants grown at LD and HD respectively.For plants from both treatments hemicellulose contents of husks, leaves and whole shoots increased with age whereas those of stems decreased. Cellulose contents did not vary greatly over the growing season.On ensiling plants from both densities, total soluble sugars had virtually disappeared after only 3 days. Apparent losses (approximately 0·3 g/g), over a longer period of time, were observed in hemicellulose sugars and starch. Apparent increases were observed in cellulose contents of approximately 0·15 g/g.

2018 ◽  
Vol 24 (4) ◽  
pp. 435-442 ◽  
Author(s):  
Drucylla Guerra Mattos ◽  
Patrícia Duarte de Oliveira Paiva ◽  
Heloisa Helena De Siqueira Elias ◽  
Eduardo Valério De Barros Vilas Boas ◽  
Lucas Ferreira Rodrigues ◽  
...  

Floral opening stage during harvest and use of postharvest techniques, such as inflorescence coating with carnauba wax, may influence quality maintenance for commercialization. The aim was to evaluate the carbohydrate content of torch ginger inflorescences harvested at two different opening stages and treated with different concentrations of carnauba wax. The inflorescences were harvested with semi-open (basal bracts beginning their expansion process) and open bracts (fully expanded basal bracts and opening of the smaller bracts on interior of the inflorescence) and received the application of carnauba wax at concentrations of 0.75%; 1.5% or 3.0%, in addition to a control treatment, without wax application. After the treatment, the floral stems were maintained at 16 and 21 oC for 20 days. During the storage period, five bracts samples (external and internal bracts separately - in open inflorescences, external and internal bracts together - in semi-open inflorescences) were carried out every three days for evaluation of total soluble sugars and starch content. Contents of total soluble sugars and starch differed between the different types of bracts collected and throughout the storage period evaluated, and could indicate a remobilization of reserves. The concentration of 3.0% carnauba waxinduced higher total soluble sugar content. However, this content does not affect the longevity of torch ginger at the two evaluated floral opening stages.


2016 ◽  
Vol 22 (2) ◽  
pp. 147 ◽  
Author(s):  
Lucas Cavalcante Da Costa ◽  
Fernando Luiz Finger

Vase life is one of the most important characteristics in the quality of cut flowers. The carbohydrate content of petals from each cultivar can be a decisive factor to vase life of gladiolus flowers. Despite exposure to ethylene does not affect the life of open florets of gladiolus flowers, it may reduce the flower’s commercial life due the occurrence of flower buds abortion. This study thus aimed to evaluate the flower opening and the vase life of gladiolus flowers according to ethylene sensitivity and carbohydrate content of each cultivar. The gladiolus cultivars used in Experiment I were  ‘Amsterdam’, ‘Blue Frost’, ‘Gold Field’, ‘Green Star’, ‘Jester’, ‘Lavender’, ‘Red Beauty’, ‘Rose Supreme’, ‘Traderhorn’, and ‘Verônica’. Stems were stored in airtight chambers and exposed to the ethylene concentrations of 0, 0.1, 1.0, 10, 100, and 1000 μL L−1 for 24 h. Subsequently, flower stem were placed in a test tube with distilled water when were evaluated for percentage of flower opening and vase life. In Experiment II, three cultivars (‘Amsterdam’, ‘Red Beauty’, and ‘Verônica’) were chosen based on the lowest, medium, and highest vase life in Experiment I, respectively. Total soluble sugars, reducing sugars, and starch contents were evaluated in five flower developmental stages. No effect of ethylene on the flower opening or alterations in flower opening between cultivars. The vase life of gladiolus flowers differed between cultivars regardless of ethylene action. ‘Verônica’ and ‘Amsterdam’, which had the highest and lowest vase life, respectively, differed as to the total soluble sugar content. Ethylene does not influence the flower opening or vase life of the gladiolus cultivars studied. In these cultivars, the maintenance of flower opening is attributed to total soluble sugars content, which, in turn, is a major determinant of the vase life.


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 685a-685
Author(s):  
X. Liu ◽  
P. Robinson ◽  
M.L. Arpaia ◽  
G.W. Witney

Monthly samples were taken from 9-year-old `Hass' avocado trees on Duke 7 rootstock grown at the UC Southcoast Research and Extension Center in Irvine, Calif. Changes in starch and total soluble sugars were monitored from fine and coarse roots, trunk (above the bud union), small diameter stems, leaves, and fruit. When possible, seasonal carbohydrate changes were compared to root and shoot flushing patterns. In all of the vegetative plant organs monitored, total soluble sugars accounted for most of the carbohydrate. Starch accounted for ≈10% of the sample dry weight, whereas the total soluble sugars accounted for ≈18%. D-mannoheptulose and perseitol, both C7 sugars, were the predominant soluble sugars throughout the year. Fructose, glucose, and sucrose accounted for <5% of the total soluble sugars. During fruit development, soluble sugar content of the exo- and mesocarp tissues >25% of the dry weight. The significance of these findings will be discussed in relationship to tree phenology and carbohydrate partitioning.


2021 ◽  
Author(s):  
Lynn Doran ◽  
Amanda P. De Souza

Quantification of total soluble sugars (as glucose) in plant tissue extracts via the sulfuric phenol method adapted for 96 well plates.


2021 ◽  
Vol 20 (1) ◽  
pp. 71-80
Author(s):  
Anousheh Zakeri ◽  
Ramazan-Ali Khavari-Nejad ◽  
Sara Saadatmand ◽  
Fatemeh Nouri Kootanaee ◽  
Rouzbeh Abbaszadeh

The effects of magnetic waves as natural environmental factors on the Earths are not well known on plant growth and development. The present study was carried out to evaluate the effects of static magnetic field (SMF) treatment (4 and 6 mT for 30 and 120 min per day) for eight days on the biomass production, proline contents and total soluble sugar, phenolic compounds, accumulation of H2O2 and MDA along with activity of antioxidant enzymes in lemon balm seedlings. Our results showed that SMF treatments, especially 6 mT and 120 min duration, increased the plant biomass, proline contents, phenolic compounds, H2O2 and MDA accumulation, and reduced the contents of total soluble sugars. The SMF application also increased the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes compared to untreated seedlings. Our results suggest that SMF treatments induces the antioxidant defense system in the lemon balm seedlings and, by changing the plant metabolism, improves the early vigor of seedlings.


2016 ◽  
Vol 2 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Md Moniruzzaman Sohag Howlader ◽  
Sheikh Rashel Ahmed ◽  
Khadizatul Kubra ◽  
Md Khairul Hassan Bhuiyan

The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.Asian J. Med. Biol. Res. March 2016, 2(1): 121-130


2000 ◽  
Vol 10 (3) ◽  
pp. 393-396 ◽  
Author(s):  
Nathalie Chabrillange ◽  
Stéphane Dussert ◽  
Florent Engelmann ◽  
Sylvie Doulbeau ◽  
Serge Hamon

AbstractLarge differences in seed desiccation sensitivity have been observed previously among ten coffee species (Coffea arabica, C. brevipes, C. canephora, C. eugenioides, C. humilis, C. liberica, C. pocsii, C. pseudo-zanguebariae, C. sessiliflora and C.stenophylla). Of these species,C. libericaandC. humiliswere the most sensitive to desiccation andC. pseudozanguebariaethe most tolerant. A study was carried out using the same seed lots to investigate if these differences in desiccation tolerance could be correlated with differences in soluble sugar content. Soluble sugars were extracted from dry seeds and analysed using high performance liquid chromatography. The seed monosaccharide (glucose and fructose) content was very low (1.5 to 2 mg g-1dry weight [dw]) in all species studied. The sucrose content ranged from 33 mg g-1dw inC. libericaseeds to 89 mg g-1dw in seeds ofC. pocsii. Raffinose was detected in the seeds of only five species (C.arabica, C.brevipes, C.humilis, C.sessiliflora, C.stenophylla), among which only three species (C.arabica, C.sessilifloraandC.brevipes) also contained stachyose. Both raffinose and stachyose were present in very low quantities (0.3–1.4 mg g-1dw and 0.1–0.7 mg g-1dw, respectively). Verbascose was never detected. No significant relationship was found between seed desiccation sensitivity and: (i) the sugar content; (ii) the presence/absence of oligosaccharides; and (iii) the oligosaccharide:sucrose ratio.


1978 ◽  
Vol 58 (1) ◽  
pp. 199-206 ◽  
Author(s):  
M. TOLLENAAR ◽  
T. B. DAYNARD

Kernel development was studied in the maize (Zea mays L.) hybrids United-H106 and Funk’s G-4444, grown in a controlled-environment growth room. A method was employed in which husks were excised, and kernels were removed from the same set of ears at several subsequent sampling dates. This method did not affect the dry matter accumulation of the remaining kernels. Basal kernels (kernel numbers 6–15 in the row) and tip kernels (kernel numbers 31–40) were removed at 2-day intervals during the period from 10 to 20 days postsilking. Dry weight, ethanol-soluble sugar content, and starch content were determined for each sample. Accumulation of dry matter in the tip kernels ceased in a fraction of the United-H106 ears at the onset of the period of linear tip-kernel dry matter accumulation. Only small differences were observed in sugar content between growing and non-growing tip kernels of ears of United-H106. Starch appeared to continue to accumulate in kernels in which dry matter had ceased to accumulate. Except for a delay of approximately 2 days, the pattern of development of tip kernels in Funk’s G-4444 was similar to that of kernels at the base.


Author(s):  
Thang Thanh Tran ◽  
Trinh Thi Diem Phan ◽  
Huong Thanh Tran

In this study, NaCl at varrious concentrations of 4 – 10 g/L was used to investigate the salt tolerance of in vitro shoot cuttings of Chrysanthemum indicum. Morphological, physiological and biochemical changes during the response of shoot cuttings in the salinity stress were analyzed. NaCl at 6 g/L reduced the development of shoot cuttings. Under salinity stress conditions, there have just a little reduction of the chloroplast in parenchymal cells near the midrib of leaf before they turn brown and die. Besides, carotenoid, starch content, and photosynthesis intensity were decreased. In contrast, respiration rate, proline and total soluble sugar content, and the activity of IAA and gibberellin were strongly increased. The application of IAA 0.25 mg/L, zeatin 0.1 mg/L and GA3 0.1 mg/L improved the shoot development in the salinity stress condition. Shoots in MS medium supplemented with BA 0.2 mg/L, NAA 2 mg/L and NaCl 6 g/L grow better in salinity stress condition.


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 648d-648
Author(s):  
Jack W. Buxton ◽  
Donna Switzer ◽  
Guoqiang Hou

Marigold seedlings, 3 weeks old, were grown in natural light growth chambers at 3 day/night temperature regimes, 8°N/16°D, 13°N/20°D and 18°N/24°D, in a factorial combination with ambient and 1000-1500 ppm CO2. Seedlings were harvested at regular intervals during a 24 hr period and were analyzed for soluble sugars (reducing sugars and sucrose) and starch. Neither temperature nor CO2 concentration affected the accumulation of soluble sugars or starch during the day or night. The soluble sugar concentration ranged from 3% of dry weight at sunrise to 6% at mid-day; the concentration changed little during the night. Light intensity was different during replications of the experiment. Increased light intensity appeared to cause a slight increase in the soluble sugars maintained by the seedling during the day. Accumulated starch increased 6% to 8% from sunrise to late afternoon. Preliminary results indicate that light intensity greatly affected the concentration of starch. On the higher light intensity day, starch accumulated to a maximum of 18% of dry weight; whereas on the lower light intensity day the maximum concentration was 10%. During the night following the lower light intensity day, the starch concentration decreased to approximately 3% by the end of the night; following a brighter day the starch content was 13% at the end of the night.


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