scholarly journals In vivo Micrografts in Almond and Their Application in Breeding Programs

2001 ◽  
Vol 11 (2) ◽  
pp. 313-315 ◽  
Author(s):  
P. Martínez-Gómez ◽  
T.M. Gradziel

The techniques of micrografting provide useful tools for the early propagation of breeding lines in variety improvement programs. In this study, the success of various in vivo micrografts of buds of `Nonpareil' almond (Prunus dulcis) seedlings have been examined. Variables tested include type of microscion, the rootstock genotype, and the growth stage of the rootstock. Microscions tested included small [3 mm (0.12 inches)] microwedges from either unsprouted or recently sprouted buds. Approach micrografts were also evaluated. Three-month-old woody rootstocks tested included `Nemaguard' and `Nemared' peach (P. persica) rootstocks, and the `Hansen' (peach × almond) hybrid rootstocks. `Nemared' rootstocks were also grafted after 1 month of growth when the tissue was still herbaceous. Micrografts onto woody rootstocks and using recently sprouted buds showed the best results. With very young or weak seedlings, where the recovery of microscions was difficult, the use of approach micrografts onto herbaceous rootstock proved a useful technique.

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 477C-477
Author(s):  
Pedro Martinez-Gomez ◽  
Mary Ann Thorpe ◽  
Thomas M Gradziel

Micrografts have proven a very useful technique when the early propagation of plant material is desired either to invigorate weak material, multiply selected genotypes, or obtained virus-free shoots. This is the situation with the recovery of haploid almond embryos, which occur at low frequency with sexual embryos in twin seeds (i.e., multiple embryos within the same seedcoat). Often these haploid plants show weak growth due to their haploid condition and their poorly developed state within twin seeds. Very little information is presently available, however, concerning the effectiveness of different micrografting techniques for almond. In this work, we examine the success of in vivo micrografting of `Nonpareil' almond seedlings under different conditions. Variables included type of micro-scion, the rootstock genotype, and the growth stage of the rootstock. Microscions tested included small (3 mm) micro-wedges from either unsprouted or recently sprouted buds. Rootstocks evaluated included the `Hanson' (peach × almond) hybrid, and Nemared and Nemaguard peach rootstocks. Rootstocks were grafted after either ≈3 weeks of growth, when the tissue was still herbaceous, or after ≈3 months of growth, when the tissue had become woody. Results show significant differences between the treatments. Findings will be discussed both in terms of effectiveness of different approaches and the advantages and disadvantages of their use in breeding programs.


2019 ◽  
Vol 11 (23) ◽  
pp. 2752 ◽  
Author(s):  
Xiaoyan Zhang ◽  
Jinming Zhao ◽  
Guijun Yang ◽  
Jiangang Liu ◽  
Jiqiu Cao ◽  
...  

Yield evaluation of breeding lines is the key to successful release of cultivars, which is becoming a serious issue due to soil heterogeneity in enlarged field tests. This study aimed at establishing plot-yield prediction models using unmanned aerial vehicle (UAV)-based hyperspectral remote sensing for yield-selection in large-scale soybean breeding programs. Three sets of soybean breeding lines (1103 in total) were tested in blocks-in-replication experiments for plot yield and canopy spectral reflectance on 454~950 nm bands at different growth stages using a UAV-based hyperspectral spectrometer (Cubert UHD185 Firefly). The four elements for plot-yield prediction model construction were studied respectively and concluded as: the suitable reflectance-sampling unit-size in a plot was its 20%–80% central part; normalized difference vegetation index (NDVI) and ration vegetation index (RVI) were the best combination of vegetation indices; the initial seed-filling stage (R5) was the best for a single stage prediction, while another was the best combination for a two growth-stage prediction; and multi-variate linear regression was suitable for plot-yield prediction. In model establishment for each material-set, a random half was used for modelling and another half for verification. Twenty-one two growth-stage two vegetation-index prediction models were established and compared for their modelling coefficient of determination (RM2) and root mean square error of the model (RMSEM), verification RV2 and RMSEV, and their sum RS2 and RMSES. Integrated with the coincidence rate between the model predicted and the practical yield-selection results, the models, MA1-2, MA4-2 and MA6-2, with coincidence rates of 56.8%, 58.5% and 52.4%, respectively, were chosen for yield-prediction in yield-test nurseries. The established model construction elements and methods can be used as local models for pre-harvest yield-selection and post-harvest integrated yield-selection in advanced breeding nurseries as well as yield potential prediction in plant-derived-line nurseries. Furthermore, multiple models can be used jointly for plot-yield prediction in soybean breeding programs.


1996 ◽  
Vol 47 (1) ◽  
pp. 1 ◽  
Author(s):  
LDJ Penrose ◽  
M Mosaad ◽  
TS Payne ◽  
G Ortiz-Ferrara ◽  
HJ Braun

This study sought to compare developmental controls in breeding a within two winter wheat improvement programs, one Australian and a CIMMYT/ICARDA program based in West Asia. Developmental controls considered were intrinsic earliness, and responses to photoperiod and to vernalization. The reliability with which each control on development had been measured was tested in separate experiments using the wheats utilized in the Australian program. Measures of intrinsic earliness showed significant agreement between experiments, better agreement being found for response to photoperiod and between integrated response to vernalization and time to double ridge after late summer sowings. The wheats utilized in the CIMMYTI/CARDA programs were found to be quick in intrinsic earliness, and to possess little response to photoperiod. While these controls varied more for the wheats utilized in the Australian program, commercial Australian winter wheats were similar to the CIMMYTI/CARDA lines. Lines utilized by both programs were represented by types with spring, facultative and winter habit. The clearest differences between programs were that CIMMYTI/CARDA winter wheats appeared to have much stronger response to vernalization than the Australian winter wheats. These findings suggest breeders would find a good proportion of segregates, from crosses between the Australian and the CIMMYTI/CARDA winter wheats, to be developmentally adapted to south-central New South Wales. This suggests CIMMYTI/CARDA winter wheats provide a matching pool from which to access germplasm to introduce new characters into Australian winter wheats.


1999 ◽  
Vol 26 (2) ◽  
pp. 100-106 ◽  
Author(s):  
A. K. Culbreath ◽  
J. W. Todd ◽  
D. W. Gorbet ◽  
S. L. Brown ◽  
J. A. Baldwin ◽  
...  

Abstract Epidemics of tomato spotted wilt, caused by tomato spotted wilt Tospovirus (TSWV), were monitored in field plots of runner-type peanut (Arachis hypogaea L.) cultivars Georgia Green and Georgia Runner and numerous breeding lines from four different breeding programs as part of efforts toward characterizing breeding lines with potential for release as cultivars. Breeding lines were divided into early, medium and late maturity groups. The tests were conducted near Attapulgus, GA and Marianna, FL in 1997 and in Tifton, GA and Marianna, FL in 1998. Epidemics in some early and medium maturing breeding lines, including some genotypes with high oleic acid oil chemistry, were comparable to those in Georgia Green, the cultivar most frequently used in the southeastern U.S. for suppression of spotted wilt epidemics. No early maturing breeding lines had lower spotted wilt final intensity ratings or higher yields than Georgia Green. However, spotted wilt intensity ratings in some late maturing lines and a smaller number of medium maturing lines were significantly lower than those of Georgia Green. Several of those lines also produced greater pod yields than Georgia Green. Results from these experiments indicated that there is potential for improving management of spotted wilt though development of cultivars that suppress spotted wilt epidemics more than currently available cultivars. There was no indication that differences in spotted wilt ratings corresponded to differences in numbers of thrips adults or larvae.


2015 ◽  
Vol 2 ◽  
pp. 81 ◽  
Author(s):  
Gilberto Antonio Peripolli Bevilaqua ◽  
Iraja Ferreira Antunes

The common bean has been object of breeding programs aiming the development of new cultivars adapted to varied production system and shown differentiated nutritional characteristics. Due a genetic diversity existent the landraces can be used directly for cropping, for present characteristics desirable. Little information exists about mineral content and other quality traits for those bean landraces. The aim of this paper was to verify the variability for grain nutricional caracters in breeding cultivars and landraces of bean from Rio Grande do Sul state, Brazil. The experiment was conducted in 2009/2010 in Experimental Station Cascata, of Embrapa Temperate Agriculture. In whole grain of 54 bean genotypes with black and no black coat were determined macroelements (nitrogen, phosphorus, potash, calcium, magnesium and sulfur), oligoelements (iron, manganese, zinc and cuprum), protein and ash content, insoluble fiber, digestive nutrient and antioxidant astragalina. The results shown that the landraces varieties presents nutritional composition of macro and oligoelements, fibers, protein and ash contents in whole grain similar than that of breeding lines and cultivars. The black coat grain from breeding programs showed better nutritional quality for macro and oligoelements content than coloured grain, highlighting TB 02-04 e TB 01-01. The landraces with coloured grains TB 02-26, TB 02-24 and TB 03-13 showed the high levels of astragaline.


Author(s):  
David Duarte-Alvarado ◽  
Tulio César Lagos-Burbano ◽  
Liz K. Lagos-Santander ◽  
Carlos-Andrés Benavides-Cardona

Lulo (Solanum quitoense) is a promising agro-industry fruit tree, not only because of its nutritional value, taste, and appearance but also because it provides an alternative production system in mild and moderately cold climate zones. Lulo crop yield and production in the Department of Nariño (Colombia) has decreased in recent years when compared to other producing regions in Colombia. Therefore, the objective of this study was to estimate the effects of the general combining ability (GCA) and the specific combining ability (SCA) in a diallel cross of 10 promising parents in four growing regions of the Department of Nariño for use in breeding programs. A total of 45 hybrid combinations were obtained and assessed with Griffing method 4. The following variables were assessed: days to flowering onset (DFO), number of clusters per branch (NCB), fruit weight (FW), polar axis (PA) of the fruit, and yield (Y). The analysis of variance showed statistical differences for most variables in response to single-cross hybrid effects and locations, except for NCB and Y. Moreover, significant differences were found for the interactions between the GCA and SCA and the hybrids and locations, respectively, meaning that environment must be considered when selecting parents with specific adaptability. The effects of the GCA and SCA promoted higher positive values for the FW and Y in parents 4, 6, and 8 and their combinations. Therefore, these parental genotypes are promising for lulo genetic improvement programs since their additive effects and genetic dominance favor fruit weight and yield.


2011 ◽  
Vol 11 (spe) ◽  
pp. 16-26 ◽  
Author(s):  
Luiz Antônio dos Santos Dias

The paper analyses the puzzle of the food-energy-environmental security interaction, to which biofuels are part of the solution. It presents and discusses the contribution of genetic improvement to biofuels, with regard to the production of raw materials (oil and ethanol-producing plant species) and designs perspectives, opportunities, risks and challenges, with a special focus on the Brazilian scene. Bioethanol is a consolidated biofuel owing largely to the sugarcane breeding programs. These programs released 111 sugarcane cultivars and were responsible for a 20.8 % gain in productivity of bioethanol (in m³ ha-1) between 2000 and 2009. The program of Brazilian biodiesel production, initiated in 2005, had an annual growth rate of 10 % and the country is already the world's fourth largest producer. However, the contribution of breeding to biodiesel production is still modest, due to the lack of specific improvement programs for oil.


2008 ◽  
Vol 35 (2) ◽  
pp. 81-85 ◽  
Author(s):  
S. P. Tallury ◽  
H. E. Pattee ◽  
T. G. Isleib ◽  
H. T. Stalker

Abstract Several diploid wild species of the genus Arachis L. have been used as sources of resistance to common diseases of cultivated peanut (Arachis hypogaea L.). Because flavor is among the most important quality attributes for commercial acceptance of roasted peanuts, sensory attributes of interspecific hybrid derived breeding lines were evaluated to determine if transfer of disease resistance from wild species is associated with concomitant changes in flavor. Sixteen interspecific hybrid derivatives with five diploid species in their ancestries and the commercial flavor standard, NC 7 were evaluated for sensory quality. Significant variation among entries was found for the roasted peanut, sweet, and bitter sensory attributes, but not for the overall contrast between NC 7 and the wild species-derived breeding lines. The variation was either between two groups of wild species-derived breeding lines or within one or both groups. Introduction of disease and pest resistance traits from Arachis species did not result in degradation or improvement of the flavor profile. This suggests that flavor of wild species-derived germplasm will not prevent its use either as parents in peanut breeding programs or as cultivars.


Plant Disease ◽  
2022 ◽  
Author(s):  
Francisco Beluzán ◽  
Xavier Miarnau ◽  
Laura Torguet ◽  
Lourdes Zazurca ◽  
Paloma Abad-Campos ◽  
...  

Twenty-five almond cultivars were assessed for susceptibility to Diaporthe amygdali, causal agent of twig canker and shoot blight disease. In laboratory experiments, growing twigs were inoculated with four D. amygdali isolates. Moreover, growing shoots of almond cultivars grafted onto INRA ‘GF-677’ rootstock were used in four-year field inoculations with one D. amygdali isolate. In both type of experiments, inoculum consisted of agar plugs with mycelium, which were inserted underneath the bark and the lesion lengths caused by the fungus were measured. Necrotic lesions were observed in the inoculated almond cultivars both in laboratory and field tests, confirming the susceptibility of all the evaluated cultivars to all the inoculated isolates of D. amygdali. Cultivars were grouped as susceptible or very susceptible according to a cluster analysis. The relationship between some agronomic traits and cultivar susceptibility was also investigated. Blooming and ripening times were found relevant variables to explain cultivars performance related to D. amygdali susceptibility. Late and very late blooming, and early and medium ripening cultivars were highly susceptible to D. amygdali. Our results may provide valuable information that could assist in ongoing breeding programs of this crop and additionally in the selection of cultivars for new almond plantations.


2000 ◽  
Vol 125 (2) ◽  
pp. 265-270 ◽  
Author(s):  
A.M.S. Nyomora ◽  
P.H. Brown ◽  
K. Pinney ◽  
V.S. Polito

The effect of boron (B) on in vivo and in vitro development of almond [Prunus dulcis (Mill.) D.A. Webb (syn. P. amygdalus Batsch)] pollen and pollen tubes and the resultant effect on fruit set was studied in mature trees. The cultivars Mono (pistil donor) and Butte (pollinizer) in an orchard with low soil B in Fresno, California were sprayed with B at 0, 0.8, 1.7, or 2.5 kg·ha-1 during Fall 1993. Pollen viability as indicated by the fluorescein diacetate method (FDA) was >85% and was not affected by field-applied B, however, in vivo pollen germination and tube growth were enhanced by foliar-applied B. More effect of applied B on in vivo growth appeared as pollen tubes progressed toward the ovary. For in vitro germination, foliar-applied B reduced bursting of tubes, and addition of B to the culture media significantly increased pollen germination and pollen tube growth.


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