scholarly journals Solid Matrix Priming Hastens Canterbury Bells Seed Germination

2002 ◽  
Vol 12 (2) ◽  
pp. 268-270 ◽  
Author(s):  
Theresa L. Bosma ◽  
Janet C. Cole ◽  
Kenneth E. Conway ◽  
John M. Dole
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Solid Matrix ◽  
Curvilinear Relationship ◽  
Calcined Clay ◽  
Water Potentials

Canterbury bells (Campanula medium `Champion Blue') seeds were primed using calcined clay at 68 °F (20 °C) for 1, 3, or 5 days at water potentials (Ψ) of -25, -20, -18, or -16 bars (-2.5, -2.0, -1.8, or -1.6 MPa). Germination was fastest (3.0 to 3.1 days) after priming with a Ψ of -18 or -16 bars for 5 days. Seeds primed for 3 or 5 days with moisture present germinated faster than nonprimed seeds, but time to 50% germination (T50) was longer when seeds were primed for 1 day regardless of Ψ compared to nonprimed seed. Germination uniformity decreased (time from 10% to 90% germination, T10-90, increased) as Ψ increased. Although a curvilinear relationship existed between T10-90 and priming duration, T10-90 did not differ between nonprimed seeds and seeds in any priming treatment except those primed for 3 days with 20% moisture (-16 bars). Priming did not affect total germination percentage (97%).

scholarly journals Enhanced Celery Germination at Stress Temperature via Solid Matrix Priming

HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 20-22 ◽  
Author(s):  
Carlos A. Parera ◽  
Ping Qiao ◽  
Daniel J. Cantliffe
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Germination Percentage ◽  
Apium Graveolens ◽  
Solid Matrix ◽  
Calcined Clay ◽  
Celery Seed ◽  
Negative Effect ◽  
Celery Seeds

To alleviate high-temperature-induced reductions in seed germination, emergence, and seedling uniformity in celery (Apium graveolens L.), seeds were primed via solid matrix priming (SMP); 0.5 g celery seeds was incubated at 15C with 10 g calcined clay and 2 ml water. After 2 days, 1, 2, 3, or 4 ml water or 1% NaOCl solution was added and seeds were kept for 2, 4, 6, 8, 10, 12, and 14 additional days. The germination percentage and coefficient of germination velocity (COV) were calculated for each treatment at 15 and 30C. The seeds primed with NaOCl gained significantly less moisture after 8 days of priming than those treated with water, regardless of the volume added. Germination of nonprimed seeds was 83% and 2% at 15 and 30C, respectively. Final germination at 30C increased to >80% when seeds were primed with 3 or 4 ml NaOCl solution or 3 ml water for >10 days. SMP treatment significantly reduced the negative effect of high temperature on celery seed germination.

scholarly journals 229 Solid Matrix Priming of Gourd Seeds for Fast and Uniform Germination

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 481E-481
Author(s):  
Hae-Jeen Bang ◽  
Soo-Jung Hwang ◽  
Hyun-Sook Ham ◽  
Jung-Myung Lee
Keyword(s):  
Seed Germination ◽  
Early Stage ◽  
Germination Percentage ◽  
Solid Matrix ◽  
Dry Heat ◽  
Heat Treated ◽  
Early Germination ◽  
Chemical Solutions ◽  
Dry Heat Treatment ◽  
Cotyledon Expansion

Grafting is common in all cucurbits in Asia, and gourd (Lagenaria siceraria) is the most popular rootstock for watermelons. Since the grafting is practiced at very early stage (right after the cotyledon expansion), uniform germination of rootstocks as well as the scions is crucial for grafting efficiency. Seeds were divided into three groups; intact, dry-heat treated (75 °C for 72 h), and brushed (575 rpm for 5 min). In each group, various solid matrix priming (SMP) treatments were imposed. Microcel E was used for SMP treatment with water or chemical solutions (10 seed: 1 Microcel E: 3 water, by weight). SMP treatment promoted earlier seed germination in all tested cultivars, thus resulting in higher rate of graftable seedlings. Brushing before SMP further enhanced earlier and uniform seed germination. Dry heat treatment, which can eliminated the seed-borne Fusarium spp. and virus, significantly delayed the early germination although the final germination percentage was not influenced. The characteristics of seedlings will also be presented.

scholarly journals Germination of Styrax camporum Pohl. seeds in response to substrate types, moisture contents and the seed morphology

2013 ◽  
Vol 85 (1) ◽  
pp. 295-306 ◽  
Author(s):  
Edson Simão ◽  
Adriana T. Nakamura ◽  
Massanori Takaki
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Riparian Forest ◽  
Field Capacity ◽  
Germination Percentage ◽  
Seed Morphology ◽  
Moisture Contents ◽  
Water Potentials ◽  
Time Periods ◽  
Substrate Types

This study evaluated the contributions of Styrax camporum seed morphology (size of seeds, presence or absence of endocarp attached to the seed), different substrates (filter paper, vermiculite, sand and the soils of cerrado s. str., cerradão and a riparian forest), different water potentials (0, -0.1, -0.2, -0.3, -0.4 and -0.5 MPa), light and temperature to seed germination. Seed size did not affect the germination percentage when seeds were sown on vermiculite. Seeds were affected by small variations in the moisture content of the tested substrates, showing a significant decrease in germination under water potentials lower than -0.1 MPa, close to the field capacity of cerrado s. str. soils. At the temperatures of 15 and 20°C, a significant decrease in germination was observed. Thus, the availability of water in cerrado soils associated to temperature modulate the distribution of germination in this species. Seed morphology contributes to the maintenance of seeds in the soil, and the lack of synchrony in seed germination spreads the distribution of germination in time. These peculiarities allow the emergency of seedlings at different time periods and establishment conditions, an adaptative response of S. camporum to the cerrado environment.

scholarly journals ENHANCED GERMINATION OF CELERY AT STRESS TEMPERATURE VIA SOLID MATRIX PRIMING

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 577e-577
Author(s):  
Daniel J. Cantliffe ◽  
Ping Oiao ◽  
Carlos A. Parera
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Dry Weight ◽  
Germination Percentage ◽  
Apium Graveolens ◽  
Solid Matrix ◽  
Time Interval ◽  
Celery Seed ◽  
Different Temperatures ◽  
Negative Effect

The high temperature severely reduces seed germination, emergence, and seedling uniformity in celery (Apium graveolens L.). Celery seeds were primed via solid matrix priming (SMP) using water or 1% sodium hypochlorite (NaOCl) solution at 1, 2, 3 or 4 ml rate for 2, 4, 6, 8, 10, 12 and 14 days. Moisture content of the seeds was calculated for each priming treatment and time interval. After priming, the seeds were dried back to the original dry weight. The germination percentage was calculated for each treatment at two different temperatures (15 and 30C). The seed primed with NaOCl gained significantly less moisture during priming than the water treatment. Seeds primed with NaOCl had significantly greater germination at both 15 and 30C, compared to seeds primed with water. The germination percentage of non-primed seeds was 83% and 2% at 15C and 30C, respectively. The final germination percentage at 30C was increase to 85% when the seeds were primed with 3 ml of NaOCl for 14 days. The combination of SMP with NaOCl significantly reduced the negative effect of high temperature on celery seed germination.

scholarly journals Effect of priming regimes on seed germination of field crops

2020 ◽  
Vol 28 (2) ◽  
pp. 169-176
Author(s):  
N. Nyoni ◽  
E. Ndlovu ◽  
M. Maphosa
Keyword(s):  
Seed Germination ◽  
Low Cost ◽  
Seed Priming ◽  
Germination Percentage ◽  
Solid Matrix ◽  
Field Crops ◽  
Volume Weight ◽  
Shallow Soils ◽  
Parameter Values ◽  
Semi Arid

Germination, early emergence and stand establishment of crops are major yield determining factors in semi-arid and arid rainfed areas. Farmers in marginal and low input areas tend to have poor germination due to poor and shallow soils. The objective of this study was to evaluate the effect of priming regimes on seed germination of field crops. A study was conducted at Lupane State University, Biotechnology Laboratory in 2019. Treatments included seed priming techniques, namely hydropriming, halopriming (2% NaCl solution) osmopriming (10% PEG 6000), and solid matrix priming (18% volume/weight sand); and timing at 6, 12, 24 hours for maize and 2, 4, 6 hours for cowpea, sorghum and millet. Solid matrix priming, followed by hydropriming significantly (P<0.05) improved germination parameters (germination percentage, daily germination, peak value and germination speed) of all crops. Halopriming and osmopriming were similar in their ineffectiveness, and resulted in the least germination parameter values. Six hour solid matrix priming in 18% v/w sand was the most effective method among most crops; suggesting that solid matrix priming is an effective and possibly low cost technology, with potential to improve germination of field crops. Key words: Seed hydration, semi-arid, solid matrix priming

scholarly journals Using mathematical models to evaluate germination rate and seedlings length of chickpea seed (Cicer arietinum L.) to osmotic stress at cardinal temperatures

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260990
Author(s):  
Sikandar Shah ◽  
Sami Ullah ◽  
Sajjad Ali ◽  
Ajmal Khan ◽  
Muhammad Ali ◽  
...  
Keyword(s):  
Seed Germination ◽  
Cicer Arietinum ◽  
Time Constant ◽  
Germination Rate ◽  
Interactive Effect ◽  
Germination Percentage ◽  
Hydrothermal Time ◽  
Time Model ◽  
Water Potentials ◽  
Cardinal Temperatures

Cicer arietinum is the 3rd most important cool season legume crop growing in vast arid and semi-arid regions of the world. A lab experiment was designed using hydrothermal time model (HTT) to investigate the chickpea seed germination (SG) behavior, cardinal temperatures and germination responses across fluctuating temperatures (Ts) and water potentials (Ψs). Seeds of chickpea var. NIFA 1995 were germinated at six constant Ts (7, 14, 21, 28, 35 and 42°C) each having the following five water potentials: 0, -0.2, -0.4–0.6 and -0.8 MPa. Germination percentage (G%) decreased significantly at (*P ≤ 0.05) from 86.7% at 28°C in -0.2 MPa to 10% in -0.2 MPa at 7°C. The germination rate (GR = 1/t50) against different T percentiles exhibited that linear increase was observed in the GR pattern above and below the To. Based on the confidence intervals of the model coefficients and (R2: 0.96), the average cardinal temperatures were 4.7, 23 and 44.2°C for the base (Tb), optimal (To) and ceiling (Tc) temperatures respectively. θT1 value was observed maximum at 28°C in -0.2 MPa and decreases with decreasing Ψ (-0.8 MPa). In comparison with control, the θT2 value was also highest in -0.2 MPa at 28°C. The thermal time (TT) concept is well fitted to germination fraction data in distilled water with an R2 value increasing 0.972. The hydro time constant (θH) increased with an increase in T to To and then decreased when T>To. The ѱb(50) irregularly varied with increasing T, σΨb was also recorded lowest (0.166 MPa) at 28°C and highest (0.457 MPa) at 7°C. Based on the statistical analysis, cardinal temperatures, hydrothermal time constant (θHTT) and germination findings the HTT gives an insight into the interactive effect of T and Ψ on seed germination time courses under varying environmental conditions.

scholarly journals POTENCIAIS HÍDRICOS E TEORES DE ÁGUA NA GERMINAÇÃO DE SEMENTES E CRESCIMENTO INICIAL DE MILHETO

2017 ◽  
Vol 15 (3) ◽  
pp. 619
Author(s):  
TATHIANA ELISA MASETTO ◽  
ELISÂNGELA LEITE VARGAS ◽  
SILVANA DE PAULA QUINTÃO SCALON
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Water Content ◽  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Dry Mass ◽  
Germination Percentage ◽  
Initial Growth ◽  
Water Potentials ◽  
Randomized Design

RESUMO – O processo germinativo se inicia com a absorção de água pelas sementes e é influenciado peladisponibilidade hídrica e pelo teor de água das sementes. Objetivou-se avaliar os efeitos de diferentes potenciais hídricose de teores de água das sementes na germinação e crescimento de plântulas de milheto. Foram utilizadas sementes comteores de água de 14,1%; 16,1%; 20,8% e 21,9% posicionadas em caixas plásticas do tipo “gerbox” sobre duas folhasde papel toalha para germinação previamente umedecidas com 10 mL de soluções de polietilenoglicol (PEG 6000) nospotenciais hídricos de 0,0; -0,1; -0,2; -0,3; -0,4 e -0,6 MPa. As sementes foram mantidas em câmara de germinação a25 °C sob luz branca constante. O delineamento experimental foi o inteiramente casualizado, em esquema fatorial 4x 5, utilizando-se quatro repetições de 50 sementes cada. As sementes foram avaliadas quanto à primeira contagem,germinação, comprimento da raiz, comprimento da parte aérea, comprimento total, massa fresca e seca de plântulas.A redução dos potenciais hídricos associada aos teores de água das sementes afeta negativamente a germinação e ocrescimento das plântulas de milheto, principalmente em sementes com o teor de água de 14,1%. As sementes com osteores de água acima de 16,1% são menos suscetíveis à redução das disponibilidades hídricas do substrato a partir dopotencial de -0,1 MPa.Palavras-chave: Pennisetum glaucum, polietilenoglicol, teor de água de sementes.WATER POTENTIAL AND MOISTURE CONTENT ON SEED GERMINATION AND INITIAL GROWTH OF PEARL MILLETABSTRACT – The germination process starts with water absorption by seeds and is influenced by the water availabilityand seeds moisture content. This work aimed to evaluate the effects of different substrate water potentials and the watercontents of the seeds on seed germination of pearl millet. The treatments comprised pearl millet seeds with 14.1%,16.1%, 20.8% and 21.9% moisture content and submitted to the water availability in the substrate of 0.0 (control); -0.1;-0.2; -0.3; -0.4 and -0.6 MPa. Seeds were sowed in two germination papers and positioned inside plastic boxes of the“gerbox” type and maintained at 25ºC. A completely randomized design in a factorial scheme 4 x 5 was used, with fourreplications of 50 seeds per treatment. The seeds were evaluated through the first counting, germination percentage,aerial part, root length, total length and fresh and dry mass of seedlings. The reduction of the water potentials associatedto the seed water contents affected negatively the seeds germination and seedlings growth of pearl millet, especially inseeds with 14.1% water content. The seeds with water content above 16.1% were less sensitive to the decrease in thewater availability of the substrate from -0.1 MPa.Keywords: Pennisetum glaucum, polyethylene glycol, seeds water content.

POTENCIAIS HÍDRICOS E TEORES DE ÁGUA NA GERMINAÇÃO DE SEMENTES E CRESCIMENTO INICIAL DE MILHETO

2016 ◽  
Vol 15 (3) ◽  
pp. 619
Author(s):  
TATHIANA ELISA MASETTO ◽  
ELISÂNGELA LEITE VARGAS ◽  
SILVANA DE PAULA QUINTÃO SCALON
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Water Content ◽  
Pearl Millet ◽  
Water Availability ◽  
Pennisetum Glaucum ◽  
Germination Percentage ◽  
Initial Growth ◽  
Water Potentials ◽  
Water Contents

RESUMO – O processo germinativo se inicia com a absorção de água pelas sementes e é influenciado pela disponibilidade hídrica e pelo teor de água das sementes. Objetivou-se avaliar os efeitos de diferentes potenciais hídricos e de teores de água das sementes na germinação e crescimento de plântulas de milheto. Foram utilizadas sementes com teores de água de 14,1%; 16,1%; 20,8% e 21,9% posicionadas em caixas plásticas do tipo “gerbox” sobre duas folhas de papel toalha para germinação previamente umedecidas com 10 mL de soluções de polietilenoglicol (PEG 6000) nos potenciais hídricos de 0,0; -0,1; -0,2; -0,3; -0,4 e -0,6 MPa. As sementes foram mantidas em câmara de germinação a 25 °C sob luz branca constante. O delineamento experimental foi o inteiramente casualizado, em esquema fatorial 4 x 5, utilizando-se quatro repetições de 50 sementes cada. As sementes foram avaliadas quanto à primeira contagem, germinação, comprimento da raiz, comprimento da parte aérea, comprimento total, massa fresca e seca de plântulas. A redução dos potenciais hídricos associada aos teores de água das sementes afeta negativamente a germinação e o crescimento das plântulas de milheto, principalmente em sementes com o teor de água de 14,1%. As sementes com os teores de água acima de 16,1% são menos suscetíveis à redução das disponibilidades hídricas do substrato a partir do potencial de -0,1 MPa.Palavras-chave: Pennisetum glaucum, polietilenoglicol, teor de água de sementes.WATER POTENTIAL AND MOISTURE CONTENT ON SEED GERMINATION AND INITIAL GROWTH OF PEARL MILLETABSTRACT – The germination process starts with water absorption by seeds and is influenced by the water availability and seeds moisture content. This work aimed to evaluate the effects of different substrate water potentials and the water contents of the seeds on seed germination of pearl millet. The treatments comprised pearl millet seeds with 14.1%, 16.1%, 20.8% and 21.9% moisture content and submitted to the water availability in the substrate of 0.0 (control); -0.1; -0.2; -0.3; -0.4 and -0.6 MPa. Seeds were sowed in two germination papers and positioned inside plastic boxes of the “gerbox” type and maintained at 25ºC. A completely randomized design in a factorial scheme 4 x 5 was used, with four replications of 50 seeds per treatment. The seeds were evaluated through the first counting, germination percentage, aerial part, root length, total length and fresh and dry mass of seedlings. The reduction of the water potentials associated to the seed water contents affected negatively the seeds germination and seedlings growth of pearl millet, especially in seeds with 14.1% water content. The seeds with water content above 16.1% were less sensitive to the decrease in the water availability of the substrate from -0.1 MPa.Keywords: Pennisetum glaucum, polyethylene glycol, seeds water content.

scholarly journals Effect of H2O-Based Low-Pressure Plasma (LPP) Treatment on the Germination of Bambara Groundnut Seeds

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 338
Author(s):  
Naeem Ahmed ◽  
Asad Masood ◽  
Kim S. Siow ◽  
M. F. Mohd Razip Wee ◽  
Rahmat Zaki Auliya ◽  
...  
Keyword(s):  
Food Security ◽  
Seed Germination ◽  
Chemical Modification ◽  
Low Pressure ◽  
Germination Percentage ◽  
Bambara Groundnut ◽  
Pressure Plasma ◽  
Low Pressure Plasma ◽  
Amine Groups

In general, seed germination is improved by low-pressure plasma (LPP) treatment using precursors such as air, nitrogen, argon, or water (H2O). Here, H2O-based LPP treatment using the optimized parameters of 10 W and 10 s improves the germination of Bambara groundnut seeds by 22%. LPP increases the wettability and roughness of the seed hilum while oxidizing the surface with carboxyl and amine groups. In this H2O-based treatment of Bambara groundnut seeds, combinatory etching and chemical modification facilitated the imbibition process and increased the germination percentage. The success of this method has the potential to be scaled up to solve food security with seeds otherwise facing germination-related issues.

scholarly journals Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Collection ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.

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