Effect of priming regimes on seed germination of field crops

Germination, early emergence and stand establishment of crops are major yield determining factors in semi-arid and arid rainfed areas. Farmers in marginal and low input areas tend to have poor germination due to poor and shallow soils. The objective of this study was to evaluate the effect of priming regimes on seed germination of field crops. A study was conducted at Lupane State University, Biotechnology Laboratory in 2019. Treatments included seed priming techniques, namely hydropriming, halopriming (2% NaCl solution) osmopriming (10% PEG 6000), and solid matrix priming (18% volume/weight sand); and timing at 6, 12, 24 hours for maize and 2, 4, 6 hours for cowpea, sorghum and millet. Solid matrix priming, followed by hydropriming significantly (P<0.05) improved germination parameters (germination percentage, daily germination, peak value and germination speed) of all crops. Halopriming and osmopriming were similar in their ineffectiveness, and resulted in the least germination parameter values. Six hour solid matrix priming in 18% v/w sand was the most effective method among most crops; suggesting that solid matrix priming is an effective and possibly low cost technology, with potential to improve germination of field crops. Key words: Seed hydration, semi-arid, solid matrix priming

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The Effect Halo-And Hydro-Priming on Seed Reserve Utilization and Seed Germination of Wheat Seeds Under Salinity Stress

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2014-0025 ◽

2014 ◽

Vol 47 (3) ◽

pp. 39-45

Author(s):

S.A. Tabatabaei

Keyword(s):

Seed Germination ◽

Salinity Stress ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Utilization Efficiency ◽

Field Crops ◽

Germination Characteristics ◽

Percentage Weight ◽

Wheat Seeds

Abstract Seed germination and seedling growth are critical stages in the life cycle of a plant, especially under adverse abiotic stresses. Seed germination negatively affected by stress conditions. Seed priming techniques have been used to increase germination characteristics and improve germination uniformity in more field crops under stressed conditions. This experimental aimed to evaluate the effect of salinity and halo-priming on seed reserve utilization and seed germination of wheat seeds. For create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, - 12 and -16 bar was used. Seeds of were pretreated with halo-priming at 10 °C for 24 h and water at 10 °C for 24 h. Our results showed that treatment×drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage was obtained from halo priming in control conditions. Thus priming improved study traits in wheat under salinity stress. Priming increased germination percentage and seed reserve utilization as compared to the unprimed seeds. The highest germination percentage and seed reserve utilization were as obtained from halo priming in control conditions.

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Effect of Seed Priming on Germination Properties and Seedling Establishment of Cowpea (Vigna sinensis)

Notulae Scientia Biologicae ◽

10.15835/nsb346338 ◽

2011 ◽

Vol 3 (4) ◽

pp. 113-116 ◽

Cited By ~ 6

Author(s):

Hamdollah ESKANDARI ◽

Kamyar KAZEMI

Keyword(s):

Laboratory Tests ◽

Low Cost ◽

Germination Rate ◽

Seedling Emergence ◽

Laboratory Data ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Seedling Vigor ◽

Contributing Factors

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.

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Enhanced Celery Germination at Stress Temperature via Solid Matrix Priming

HortScience ◽

10.21273/hortsci.28.1.20 ◽

1993 ◽

Vol 28 (1) ◽

pp. 20-22 ◽

Cited By ~ 8

Author(s):

Carlos A. Parera ◽

Ping Qiao ◽

Daniel J. Cantliffe

Keyword(s):

High Temperature ◽

Seed Germination ◽

Germination Percentage ◽

Apium Graveolens ◽

Solid Matrix ◽

Calcined Clay ◽

Celery Seed ◽

Negative Effect ◽

Celery Seeds

To alleviate high-temperature-induced reductions in seed germination, emergence, and seedling uniformity in celery (Apium graveolens L.), seeds were primed via solid matrix priming (SMP); 0.5 g celery seeds was incubated at 15C with 10 g calcined clay and 2 ml water. After 2 days, 1, 2, 3, or 4 ml water or 1% NaOCl solution was added and seeds were kept for 2, 4, 6, 8, 10, 12, and 14 additional days. The germination percentage and coefficient of germination velocity (COV) were calculated for each treatment at 15 and 30C. The seeds primed with NaOCl gained significantly less moisture after 8 days of priming than those treated with water, regardless of the volume added. Germination of nonprimed seeds was 83% and 2% at 15 and 30C, respectively. Final germination at 30C increased to >80% when seeds were primed with 3 or 4 ml NaOCl solution or 3 ml water for >10 days. SMP treatment significantly reduced the negative effect of high temperature on celery seed germination.

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Solid Matrix Priming Hastens Canterbury Bells Seed Germination

HortTechnology ◽

10.21273/horttech.12.2.268 ◽

2002 ◽

Vol 12 (2) ◽

pp. 268-270 ◽

Cited By ~ 1

Author(s):

Theresa L. Bosma ◽

Janet C. Cole ◽

Kenneth E. Conway ◽

John M. Dole

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Solid Matrix ◽

Curvilinear Relationship ◽

Calcined Clay ◽

Water Potentials

Canterbury bells (Campanula medium `Champion Blue') seeds were primed using calcined clay at 68 °F (20 °C) for 1, 3, or 5 days at water potentials (Ψ) of -25, -20, -18, or -16 bars (-2.5, -2.0, -1.8, or -1.6 MPa). Germination was fastest (3.0 to 3.1 days) after priming with a Ψ of -18 or -16 bars for 5 days. Seeds primed for 3 or 5 days with moisture present germinated faster than nonprimed seeds, but time to 50% germination (T50) was longer when seeds were primed for 1 day regardless of Ψ compared to nonprimed seed. Germination uniformity decreased (time from 10% to 90% germination, T10-90, increased) as Ψ increased. Although a curvilinear relationship existed between T10-90 and priming duration, T10-90 did not differ between nonprimed seeds and seeds in any priming treatment except those primed for 3 days with 20% moisture (-16 bars). Priming did not affect total germination percentage (97%).

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229 Solid Matrix Priming of Gourd Seeds for Fast and Uniform Germination

HortScience ◽

10.21273/hortsci.34.3.481e ◽

1999 ◽

Vol 34 (3) ◽

pp. 481E-481

Author(s):

Hae-Jeen Bang ◽

Soo-Jung Hwang ◽

Hyun-Sook Ham ◽

Jung-Myung Lee

Keyword(s):

Seed Germination ◽

Early Stage ◽

Germination Percentage ◽

Solid Matrix ◽

Dry Heat ◽

Heat Treated ◽

Early Germination ◽

Chemical Solutions ◽

Dry Heat Treatment ◽

Cotyledon Expansion

Grafting is common in all cucurbits in Asia, and gourd (Lagenaria siceraria) is the most popular rootstock for watermelons. Since the grafting is practiced at very early stage (right after the cotyledon expansion), uniform germination of rootstocks as well as the scions is crucial for grafting efficiency. Seeds were divided into three groups; intact, dry-heat treated (75 °C for 72 h), and brushed (575 rpm for 5 min). In each group, various solid matrix priming (SMP) treatments were imposed. Microcel E was used for SMP treatment with water or chemical solutions (10 seed: 1 Microcel E: 3 water, by weight). SMP treatment promoted earlier seed germination in all tested cultivars, thus resulting in higher rate of graftable seedlings. Brushing before SMP further enhanced earlier and uniform seed germination. Dry heat treatment, which can eliminated the seed-borne Fusarium spp. and virus, significantly delayed the early germination although the final germination percentage was not influenced. The characteristics of seedlings will also be presented.

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Two-Hour Magneto-Priming with Static Magnetic Fields Ranging from 65 ± 3 to 505 ± 8 mT Does Not Improve the Germination Percentage of Industrial Hemp Seed at a Sub-Optimal Germination Temperature

J ◽

10.3390/j1010017 ◽

2018 ◽

Vol 1 (1) ◽

pp. 192-196

Author(s):

Kathrin Spendier

Keyword(s):

Seed Germination ◽

Magnetic Fields ◽

Cannabis Sativa ◽

Seed Priming ◽

Germination Percentage ◽

Hemp Seed ◽

Static Magnetic Fields ◽

Beneficial Effects ◽

Industrial Hemp ◽

The U.S

Industrial hemp is a non-psychoactive variety of Cannabis sativa L., i.e., it contains less than 0.3% tetrahydrocannabinols (THC). This crop is one of historical importance in the U.S. as manufacturers seek industrial hemp as a renewable and sustainable resource for a wide variety of consumer and industrial products. To help farmers succeed, agronomic research on industrial hemp is needed. In this trial, investigations were performed to determine whether magneto-priming, a form of seed priming that involves magnetic fields, effects the germination percentage of industrial hemp seed. Beneficial effects of magnetic fields on seedling growths and germination have been reported for many different plant species. Dry industrial hemp seed was exposed to static magnetic fields ranging from 65 ± 3 to 505 ± 8 mT for 2 h prior to seed germination. Germination was performed at 13.6 ± 0.7 °C, a temperature that is representative of the germination temperatures of industrial hemp in the U.S. state of Colorado. Magneto-priming of seed had no statistically significant effect on seed germination percentage.

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The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽

10.3390/agriculture10040094 ◽

2020 ◽

Vol 10 (4) ◽

pp. 94

Author(s):

Iman Mirmazloum ◽

Attila Kiss ◽

Éva Erdélyi ◽

Márta Ladányi ◽

Éva Zámboriné Németh ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Germination Rate ◽

Petri Dish ◽

Seed Priming ◽

Germination Percentage ◽

Positive Effects ◽

Carum Carvi ◽

Early Seedling ◽

Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

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Utilization of Grape Fruit Waste-based Substrates for Seed Germination and Seedling Growth of Lemon Basil

HortTechnology ◽

10.21273/horttech03761-17 ◽

2017 ◽

Vol 27 (4) ◽

pp. 523-529 ◽

Cited By ~ 2

Author(s):

Mohammed El-Sayed El-Mahrouk ◽

Yaser Hassan Dewir ◽

Salah El-Hendawy

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Growth Parameters ◽

Low Cost ◽

Germination Rate ◽

Germination Percentage ◽

Waste Recycling ◽

Grape Fruit ◽

Fruit Waste ◽

One To One

Grape (Vitis vinifera) waste management is a major problem in juice production, but it could be transformed into a major opportunity if the waste was recycled and used as a nursery growing medium. The aim of this study was to evaluate the suitability of four composts based on squeezed grape fruit waste (SGFW), mixed with coir or vermiculite in a one-to-one ratio by volume to form 13 growing media, for seed germination and seedling growth of ‘Mrs. Burns’ lemon basil (Ocimum basilicum var. citriodora). The final germination percentage (FGP), corrected germination rate index (CGRI), survival percentage, and seedling growth of ‘Mrs. Burns’ lemon basil were the variables measured. Pure SGFW reduced seed germination and seedling growth. The medium combining pure SGFW with vermiculite in a one-to-one ratio by volume was optimal for seed germination and seedling growth; in this medium the highest FGP, CGRI, survival rate, and growth parameters were recorded. The negative effects of pure SGFW composts were eliminated by mixing all composts with coir or vermiculite. These waste recycling media are low-cost products that can be beneficially used in nurseries on a commercial scale.

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EFFECTS OF SEED PRIMING WITH SALICYLIC ACID ON ZEA MAYS SEEDLINGS GROWN UNDER SALT STRESS CONDITIONS

Journal of Humanities, Social and Management Sciences (JHSMS) ◽

10.54112/bcsrj.v2021i1.65 ◽

2021 ◽

Vol 2021 (1) ◽

Author(s):

S Iqbal ◽

Q Ali ◽

A Malik

Keyword(s):

Salicylic Acid ◽

Seed Germination ◽

Growth Traits ◽

Low Cost ◽

Seed Priming ◽

Maximum Growth ◽

Growth Stages ◽

Maize Crop ◽

Essential Nutrients ◽

Biotic Stresses

Maize crop is cultivated as the 3rd most important cereal crop after wheat and rice in Pakistan. As an exhaustive crop maize required all essential nutrients at all crop growth stages. Among essential nutrients nitrogen involved with the greenish colors of the vegetative parts, leaves production, branching and end up with drier biomass. Hydro-priming and osmo-priming are the commonly used priming techniques. In both techniques the seeds were soaked in treatments for overnight followed by air drying before sowing. To overcome several a-biotic stresses of field crops seed priming techniques have been used globally. The current study was planned to evaluate effect of different salt treatments on the growth traits of maize. Two maize verities Raka-Poshi and Pak-Afgoi were evaluated for the effect of salts stress on maize. Three salts ZnSO4, MgSO4 and NaCl were used @0.5 M and 0.25M solutions. Treatments used in the experiment were ZnSO4 (0.5M), ZnSO4 (0.25M), MgSO4 (0.5M), MgSO4 (0.25M), NaCl (0.5M), NaCl (0.25M), ZnSO4 (0.5M) + MgSO4 (0.5M), ZnSO4 (0.25M) + MgSO4 (0.5M), ZnSO4 (0.5M) + MgSO4 (0.25M), ZnSO4 (0.25M) + MgSO4 (0.25M), ZnSO4 (0.5M) + NaCl (0.5M), ZnSO4 0.25M + NaCl (0.5M), ZnSO4 (0.5M) + NaCl (0.25M), ZnSO4 (0.25M) + NaCl (0.25M), ZnSO4 (0.5M) + MgSO4 (0.5M) + NaCl (0.5M), ZnSO4 (0.25M) + MgSO4 (0.25M) + NaCl (0.25M). Maximum growth was observed in the plants that were primed with ZnSO4 used alone @ 0.5M and 0.25M or in combination with MgSO4. However, NaCl retard the growth of plant used alone or in combination with other salts. Seed priming by 30 mg/l salicylic acid solution not only found vital to enhance the uniform seed germination and rate of seed germination but also are environment friendly, simple, low cost low risk techniques and easily adoptable

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Effect of Calcium Nitrate and Potassium Nitrate Priming on Seed Germination and Seedling Vigour of Papaya (Carica papaya L.)

Journal of Horticultural Sciences ◽

10.24154/jhs.2019.v14i02.010 ◽

2019 ◽

Vol 14 (2) ◽

pp. 149-154

Author(s):

Maneesha S R ◽

Priya Devi S

Keyword(s):

Seed Germination ◽

Calcium Nitrate ◽

Seed Priming ◽

Potassium Nitrate ◽

Germination Percentage ◽

Shoot Length ◽

Potassium Ions ◽

Seedling Vigour ◽

Fresh Biomass ◽

Seedling Characteristics

The effect of seed priming with calcium nitrate Ca (NO3)2 and potassium nitrate (KNO3) on germination and seedling vigour were studied in papaya varieties. Open pollinated local (gauty) papaya seeds were soaked in Ca (NO3)2 and KNO3 solutions (10000 ppm, 15000 ppm and 20000 ppm) up to 24 hours and germination percentage and seedling characteristics were recorded. The least number of days taken for seed germination (4.33 days), the highest germination percentage (82.56 % ), the highest shoot length (14.31 cm) the highest fresh biomass (1.36 g) and dry biomass (0.174 g) were recorded in 10000 ppm Ca(NO3)2 treatment. Further, seeds of papaya varieties viz., Arka Surya, Arka Prabhat and Madhu bindhu were treated with 5000 ppm, 10000 ppm and 15000 ppm Ca (NO3)2 and observed that Arka Prabhat seeds treated with 10000 ppm Ca (NO3)2 hadtaken the least number of days for germination (4.75 days) and also the highest shoot length (25.2 cm). The results of the experiment proved the significant effect of calcium ions over potassium ions on papaya seed germination and seedling vigour.

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