scholarly journals Effects of protatranes on the germination of wheat and triticale seeds after their long-term storage

2021 ◽  
Vol 11 (2) ◽  
pp. 272-280
Author(s):  
G. I. Filipenko ◽  
S. N. Adamovich ◽  
E. N. Oborina ◽  
I. B. Rozentsveig ◽  
G. F. Safina
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Accelerated Aging ◽  
Germination Energy ◽  
Long Term Storage ◽  
High Germination ◽  
Wide Range ◽  
Soft Winter Wheat ◽  
Term Storage

The article studies the chemical compounds of a number of protatranes as biostimulants of the germination of wheat and triticale seeds after their long-term storage. The relevance of the study is determined by the need to restore the germination of unique samples of grain crops from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) (Saint Petersburg). The research objects were the seeds of different years of reproduction with various levels of original germination, as well as those with an initially high germination and subjected to accelerated aging. The experiments were conducted in the presence of the following physiologically active non-toxic protatranes 2-Me-C6H4OCH2COO- • HN+(CH2CH2OH)3 (1) and 4-Cl-C6H4SCH2COO-•HN+(CH2CH2OH)3 (2) across a wide range of concentrations (1•10-2 – 1•10-9M). At the first stage, the effect of protatrane 1 on the germination of soft spring wheat was investigated. It was observed that, under the influence of protatrane 1 at concentrations from 10-4 to 10-9 M, the seed germination and the germination energy increased by 8.7-20.0% and 4.7-8.0%, respectively. The use of protatrane 1 at higher concentrations (10-2M) resulted in a decrease in germination. Subsequently, experiments were carried out on 3 samples of soft winter wheat seeds stored for 52 years and having the germination of 19-26%. The 20-hour exposure of seeds to water solutions of protatrane 1 at concentrations of 5 • 10-7-10-9 M increased their germination by 3.7-10.0%. The best stimulating effect was achieved at a concentration of 5 • 10-7 M. At the next stage, the seeds with a high original germination (86-96%) and subjected to accelerated aging were investigated. The treatment of such samples with protatrane 1 at concentrations of 10-6 and 10-4 M turned out to be less effective: the germination and its energy either remained the same or increased insignificantly (by 3.5% and 7.5%, respectively). The treatment of triticale seeds with pro-tatranes 1 and 2 at a concentration of 5 • 10-7 M was carried out in a similar manner. It was shown that the action of protatranes 1 and 2 led to an increase in the germination by 3-7% and a significant increase in the germination energy by 7-45%. By further optimising the production process and selecting the most active protatranes, the described approach can provide for a significant recovery of seed germination.

scholarly journals Temperature Requirements for the Storage and Germination of Delphinium × cultorum Seed

HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 989-992 ◽  
Author(s):  
William J. Carpenter ◽  
Joseph F. Boucher
Keyword(s):  
Seed Germination ◽  
Seed Storage ◽  
Moisture Contents ◽  
Long Term Storage ◽  
Subzero Temperatures ◽  
Temperature Requirements ◽  
Cultivar Responses ◽  
Term Storage ◽  
Storage Temperatures

Delphinium seed germination was about equal in light or darkness. Seed total germination percentages were highest and about equal at constant 15 or 20C and alternating (12 h) 10/20C, 15/25C, or 20/30C. The most rapid and uniform germination generally occurred at constant 20C. Storing seeds dry at 2C for 3 weeks before germination reduced the days to 50% of final germination (T50) and between 10% and 90% germination (T90 - T10) but did not increase total germination. The seeds had only limited desiccation tolerance, with `Magic Fountains Lavender' having declining germination percentages at moisture contents below 7.0% and `Magic Fountains Lilac' below 6.7%. Seeds tolerated storage at low, nonfreezing or subzero temperatures, but cultivar responses differed. `Magic Fountains Lavendar' had progressively lower germination percentages as storage temperatures declined from 5 to –20C, while `Magic Fountains Lilac' germination increased. The relative humidity (RH) and temperature that delphinium seed received during long-term storage influenced germination. Germination after seed storage at 5C was higher, earlier, and more uniform than after 15 or 25C storage. The highest total germination percentages occurred following seed storage at 5C and 30% to 50% RH, the shortest T50 from 35% to 55% RH, and shortest spans (T90 - T10) from 25% to 50% RH.

scholarly journals Evaluarea potenţialului de păstrare a seminţelor mostrelor din colecţia de Triticum durum L.

2021 ◽  
Author(s):  
Lolita Melian ◽  
◽  
Liudmila Corlateanu ◽  
Victoria Mihailă ◽  
Doina Cutitaru ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Durum ◽  
Accelerated Aging ◽  
Gene Bank ◽  
Stress Factors ◽  
Long Term Storage ◽  
Dry Biomass ◽  
Seedling Roots ◽  
Term Storage

The test of accelerated aging of seeds (AAS) was applied on 7 genotypes of durum wheat, and the morphophysiological parameters of seeds and seedlings, such as dynamics of seed germination, length of seedling roots, fresh and dry biomass of seedlings, were investigated. The genotypic features of collection samples of durum wheat were identified after exposure to stress factors (high temperature and humidity). Such a grouping of genotypes according to their potential ability to preserve the viability of seeds is an important complex characteristic of collection samples when they are placed for long-term storage in a plant gene bank.

scholarly journals Investigation of the chemical composition of pumpkin pulp as a basis for soft drinks

Vestnik MGTU ◽  
2021 ◽  
Vol 24 (4) ◽  
pp. 441-449
Author(s):  
M. N. Shkolnikova ◽  
V. N. Abbazova
Keyword(s):  
Sugar Content ◽  
Meat Products ◽  
Climatic Conditions ◽  
Raw Material ◽  
Long Term Storage ◽  
Technological Potential ◽  
Wide Range ◽  
Snack Products ◽  
Term Storage

Pumpkin fruits ( Cucurbita spp.) have a number of advantages and high technological potential, thanks to almost universal cultivation in a wide range of agro-climatic conditions, the ability to long-term storage, the content of dietary fibers, pectin and other polysaccharides, carotenoids, polyphenolic substances, vitamins, which cause a wide range of physiological orientation. In the process of researching the composition of local cultivars of pumpkin and the world experience of using Cucurbita spp. in the composition of food products, the need to use this ingredient in beverage recipes has been substantiated. The content of dry substances in the pumpkin samples is from 8.18 % ("Gribovskaya") to 11.6 % ("Orange bush"). The maximum sugar content is distinguished by the varieties "Winter Sweet" (6.87 %) and "Orange Bush" (7.40 %). The content of BAS-antioxidants is (without visible difference depending on the growing region): carotenoids from 1.3 mg/100 g in "Gribovskaya" to 2.0 mg/100 g - "Orange bush"; ascorbic acid - 8.7 mg/100 g in "Rossiyanka" to 14.2 mg/100 g - "Orange bush". Today all parts of the pumpkin fruit are used: the bark is a raw material for the production of feed flour and a substrate for the cultivation of lactobacilli, the seeds are traditionally used to produce pumpkin oil and flour, the pulp of the fruit is a raw material for juice-containing products, purees, carotenoid-containing and polysaccharide extracts, pectin, concentrates of first and second dishes, snack products, pasta, bakery, confectionery and meat products, etc.

The effect of prolonged storage and periodic reproduction on the germination of red beet seeds

2020 ◽  
pp. 35-37
Author(s):  
В.Д. Богданова ◽  
М.В. Воробьев
Keyword(s):  
Shelf Life ◽  
Cultivated Plants ◽  
Seed Formation ◽  
Germination Energy ◽  
Long Term Storage ◽  
Red Beet ◽  
Duration Of Storage ◽  
Field Germination ◽  
Term Storage

Площади под свеклой в Российской Федерации колеблются в пределах 65-68 тыс. га, что составляет 7% от общей площади, занятой овощными культурами. Вопросы, связанные с продолжительностью хранения и периодического воспроизведения на всхожесть семян остаются актуальными. В работе исследованы изменения таких важнейших посевных качеств, как энергия прорастания и всхожесть семян столовой свеклы на примере сорта Двусемянная ТСХА после различных сроков хранения. Благодаря селекционной работе можно значительно увеличить сроки хозяйственной годности семян и продолжительность их биологической жизнеспособности. Цель исследований: оценка влияния сроков хранения семян столовой свеклы сорта Двусемянная ТСХА на их посевные качества (всхожесть и энергия прорастания). Для этого определяли всхожесть и энергию прорастания семян столовой свеклы различных семей сорта Двусемянная ТСХА в условиях длительного хранения, изучили полевую всхожесть семян в условиях длительного хранения, установили оптимальные сроки хранения. Полевая всхожесть отдельных семей существенно отличалась. В некоторых случаях, особенно после продолжительного хранения, разница достигала 70%. Наглядно показано, что энергия прорастания и всхожесть семян слабо изменяются в течение четырех лет при отсутствии различных обработок. Выявлено влияние погодных условий в год выращивания семян и генотипических различий семей, что рекомендуется учитывать в селекции и при производстве элитных семян. Результаты исследования свидетельствуют о высокой биологической долговечности семян свеклы (более 16 лет), даже при хранении в комнатных условиях. Это важно при репродукции, так как в результате потепления климата изменяется направленность отбора в сторону более позднеспелых форм, менее перерастающих и устойчивых к растрескиванию и деформации корнеплодов. Высокое качество семенного материала культурных растений зависит от условий формирования семян. The area under red beet in the Russian Federation ranges from 65-68 thousand ha, which is 7% of the total area occupied by vegetables. Questions related to the duration of storage and periodic reproduction for seed germination are relevant. The work investigated the changes in such important sowing qualities as the germination energy and germination of red beet seeds using the example of the Dvusemyannaya TSKHA variety, after different storage periods. Thanks to the conduct of breeding work, it is possible to significantly increase the terms of economic shelf life of seeds and the duration of their biological viability. The aim of the study was to assess the effect of the shelf life of seeds of red beet variety Dvusemyannaya TSKHA on their sowing qualities (germination and germination energy). To do this, we determined the germination and germination energy of seeds of red beet from different families of the variety Dvusemyannaya TSKHA under conditions of long-term storage, studied the field germination of seeds under conditions of long-term storage, and established the optimal storage periods. Field germination of individual families differed significantly. In some cases, especially as a result of long-term storage, the difference reached 70%. It is clearly shown that the energy of germination and germination of seeds change little over four years in the absence of various treatments. The influence of weather conditions in the year of seed cultivation and genotypic differences of families was revealed. It is recommended to take these factors into account in breeding and in the production of elite seeds. The research results presented by the author indicate a high biological longevity of beet seeds, even when stored in room conditions, which is more than 16 years. This is important for reproduction, since because of climate warming, the direction of selection changes towards later-maturing forms that are less outgrowing and resistant to cracking and deformation of root crops. The high quality of the seed material of cultivated plants depends on the conditions of seed formation.

Long-term room temperature storage of DNA molecules

Biomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 552-563
Author(s):  
R.R. Garafutdinov ◽  
A.R. Sakhabutdinova ◽  
A.V. Chemeris
Keyword(s):  
Data Storage ◽  
Room Temperature ◽  
Biological Data ◽  
Dna Molecules ◽  
Freezing And Thawing ◽  
Long Term Storage ◽  
Wide Range ◽  
Frozen Solutions ◽  
Term Storage

The simplest and most common method of long-term storage of DNA samples at present is the storage of their frozen solutions, which, however, has a number of disadvantages, including the destruction of DNA molecules during freezing and thawing, as well as energy consumption and the likelihood of losing valuable samples in the event of possible accidents. In this regard, long-term storage of DNA samples at room temperature in a dried state is preferable, especially since an even greater increase in the number of stored DNA samples is planned due to the planned preservation of non-biological data in this molecule, which is recognized at the International Economic Forum 2019 among the 10 most important innovative technologies as “DNA Data Storage” of the near future of mankind. Such storage requires the exclusion of hydrolysis and oxidation of DNA molecules under the action of water and reactive oxygen species, which can be achieved by placing DNA in an inert anhydrous atmosphere, including in the presence of additional ingredients in the form of, for example, trehalose, imitating wildlife, since it is known that this simple disaccharide, capable of vitrification, protects a wide range of anhydrobiont organisms from adverse environmental conditions. Currently, there are a number of technologies that provide long-term storage of DNA at room temperature, including those available from commercial sources, but not all problems have yet been solved, which is reflected in this review article.

scholarly journals The influence of long-term storage in liquid nitrogen on survival and pathogenicity of Phytophthora Infestans isolates

2012 ◽  
Vol 52 (4) ◽  
pp. 479-485 ◽  
Author(s):  
Sylwester Sobkowiak ◽  
Hanna Zarzycka ◽  
Jadwiga Śliwka
Keyword(s):  
Liquid Nitrogen ◽  
Virulence Factors ◽  
Initial Assessment ◽  
Long Term Storage ◽  
Paraffin Oil ◽  
Wide Range ◽  
The Mean ◽  
Nitrogen Treatment ◽  
Term Storage

Abstract A set of 14 aggressive Phythophthora infestans (Mont.) de Bary isolates with a wide range of virulence, was stored for eight years (2002-2010) in liquid nitrogen at -196°C (209 samples) and under paraffin oil at 7°C (70 test tubes). The survival rate of samples stored in liquid nitrogen was scored as 88%, 45 days after thawing. The revived isolates were passaged through potato tissues four times and tested for virulence on a set of 11 Black’s differentials. The mean number of virulence factors per isolate, of these P. infestans isolates evaluated before storage, was 7.4. The isolates stored in liquid nitrogen and under paraffin oil and passaged four times through potato tissues showed a similar mean number of virulence factors per isolate, 7.3 and 6.9, respectively. Isolates stored under paraffin oil showed no expression of factor avr5, but expression of this factor occurred in six isolates after liquid nitrogen treatment. The initial expression of this factor occurred in four isolates. Before storage, the average aggressiveness of the tested isolates was assessed as 1.7, on a 1-9 scale, where 1 means the most aggressive. After storage and after four subsequent passages through potato tissues, the mean aggressiveness of isolates stored in liquid nitrogen and under paraffin oil reached the level of 1.5 and 2.1, respectively. The mean aggressiveness was not significantly different from the level of initial assessment.

scholarly journals SEED QUALITY EVALUATION IN THE COLLECTION OF CYAMOPSIS TETRAGONOLOBA AFTER LONG¬TERM STORAGE UNDER UNCONTROLLED CONDITIONS

2020 ◽  
Vol 180 (4) ◽  
pp. 48-58
Author(s):  
N. V. Rakovskaya ◽  
O. N. Zabegayeva ◽  
E. A. Dzyubenko
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Growing Season ◽  
Cyamopsis Tetragonoloba ◽  
Monthly Temperature ◽  
Germination Energy ◽  
Long Term Storage ◽  
Seed Hardness ◽  
Term Storage

Background. Guar or clusterbean (Cyamopsis tetragonoloba (L.) Taub.) is an annual leguminous plant cultivated for feed, food and industrial purposes. Its seed endosperm is used to extract guar gum, so the guar genetic resources stored in the VIR collection are of strategic importance for the import substitution policy in Russia in the context of their prospective introduction into cultivation in the country’s southern areas. Most of the guar accessions had been preserved by VIR for 40 years or more, so the task was to assess their germination rates and restore their viability. In the process of screening, seed quality parameters of the accessions were examined and analyzed.Materials and methods. VIR’s collection of C. tetragonoloba, initiated by N. I. Vavilov, now contains 111 accessions. Percentage seed viability, germination energy and seed hardness were evaluated in the Long-Term Storage Laboratory for 50 accessions reproduced in 2018 and for 263 older reproductions of 89 guar accessions. In total, 313 seed samples reproduced across 18 different years were analyzed. Statistica 10 software was used to perform a single-factor analysis of variance and find out how germination energy, seed viability and seed hardness correlated with the age of seeds, mean monthly temperature and mean rainfall during the growing season.Results and discussion. After more than 40 years of storage, the guar accessions did not entirely lost their viability under laboratory and field conditions. Very high positive correlations were found for their percentage viability and germination energy with the mean monthly temperature in the year of reproduction. A weak positive correlation was observed between seed hardness and the year of reproduction. Correlations between seed viability and rainfall during the growing season were weak, with a weak negative correlation between seed hardness and rainfall. The viability level of 30-year-old seeds was 50%, so guar may be regarded as belonging to the group of macrobiotic plants.

scholarly journals Effect of harvesting time on yield and sown quality of spring cereals during storage

2021 ◽  
Vol 36 ◽  
pp. 05001
Author(s):  
Olga Snigireva
Keyword(s):  
Harvesting Time ◽  
Seed Material ◽  
Laboratory Conditions ◽  
Germination Energy ◽  
Long Term Storage ◽  
Grain Moisture ◽  
The Impact ◽  
Term Storage

It is important to correctly determine the stage of harvesting. To establish full ripeness, it is necessary to accurately identify the period when plastic substances stop entering the grain. All this is necessary for the formation of high-quality grain. Due to the long-term storage of grain, its germination decreases, as there are violations in the processes of metabolism. Therefore, during the storage period, it is necessary to control the quality of the seed material. The purpose of the research was to determine the impact of harvesting time and long-term storage of grain for obtaining seed material with high indicators of yield and sowing qualities. The experimental part of the work was carried out on the experimental field and in the laboratory conditions of the FGBNU FANC North-East in 2015-2020. From each variant of the experiment, 1 kg of seeds of the 2015 harvest were stored. Under laboratory conditions, the germination energy and laboratory germination capacity of the seeds were tested. In 2020, field germination and yield qualities of seeds were studied in three-fold repetition on meter plots. During the years of research, changes in the sowing qualities of seeds obtained during storage in 2015 were noted. Seeds from crops that were harvested at the onset of the full ripeness phase and at a grain moisture content of 17 … 20 %, (1), the indicators of germination energy and germination were the highest than when harvested at a later time. A significant decrease in indicators was noted in the cleaning options for 10 and 15 days later than the optimal one. The study of the influence of the harvest year and the duration of seed storage showed that seeds 1 and 2 of the harvest period in 2015 provide a higher yield than seeds 3 and 4 of the harvest period. The use of late-harvest seeds in sowing leads to a decrease in the weight of 1000 grains and the nature of the grain.

scholarly journals UK Government Web Continuity: Persisting Access through Aligning Infrastructures

2009 ◽  
Vol 4 (1) ◽  
pp. 107-124 ◽  
Author(s):  
Amanda Spencer ◽  
John Sheridan ◽  
David Thomas ◽  
David Pullinger
Keyword(s):  
Building Blocks ◽  
Access To Information ◽  
Parliamentary Debates ◽  
Long Term Storage ◽  
Wide Range ◽  
The Government ◽  
The Uk ◽  
Term Storage ◽  
The Web

Government's use of the Web in the UK is prolific and a wide range of services are now available though this channel. The government set out to address the problem that links from Hansard (the transcripts of Parliamentary debates) were not maintained over time and that therefore there was need for some long-term storage and stewardship of information, including maintaining access. Further investigation revealed that linking was key, not only in maintaining access to information, but also to the discovery of information. This resulted in a project that affects the entire  government Web estate, with a solution leveraging the basic building blocks of the Internet (DNS) and the Web (HTTP and URIs) in a pragmatic way, to ensure that an infrastructure is in place to provide access to important information both now and in the future.

The effect of storage temperature and duration on northern red oak acorn viability and vigour

2013 ◽  
Vol 89 (06) ◽  
pp. 769-776 ◽  
Author(s):  
Thomas L. Noland ◽  
Andrée E. Morneault ◽  
Daniel C. Dey ◽  
Dave Deugo
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Red Oak ◽  
Northern Red Oak ◽  
Root Regrowth ◽  
Long Term Storage ◽  
High Germination ◽  
Percent Germination ◽  
Term Storage

Three separate collections of Ontario sources of northern red oak (Quercus rubra L.) acorns were made to determine the effects of long-term cold storage at +2°C, −1°C, and −2°C on their viability and vigour. We measured acorn moisture content, percent germination during storage, speed of germination and total germination values, root regrowth of seeds that germinated in storage, incidence of fungal contamination, and condition of ungerminated acorns. Viability and vigour peaked six to 12 months after acorns were placed in storage, but decreased with continued storage. After 18 months in storage, ≥60% of the acorns germinated in four of the five seedlots tested and, after 30 months in storage, ≥53% of the acorns germinated in three of the five seedlots tested. Acorn viability was only minimally affected by storage temperature; however, since temperatures above −2°C allowed acorns to germinate during storage, the preferred long-term storage temperature was −2°C. Our results suggest that, assuming proper storage conditions, most red oak seedlots will maintain relatively high germination levels when stored at −2°C for 18 months. Seedlots with particularly high initial germination and vigour may be successfully stored for up to 30 months.

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