scholarly journals Modern opportunities of pharmacological effect on gut microbiome and motor activity

2021 ◽  
pp. 200-208
Author(s):  
V. N. Drozdov ◽  
E. V. Shikh ◽  
A. A. Astapovskiy ◽  
S. Yu. Serebrova ◽  
I. A. Komissarenko
Keyword(s):  
Hepatic Encephalopathy ◽  
Intestinal Microflora ◽  
Functional Constipation ◽  
Current Data ◽  
Beneficial Bacteria ◽  
Drug Of Choice ◽  
Wide Range ◽  
Intestinal Peristalsis ◽  
Women And Children ◽  
Hydrolysis Of

Currently, lactulose is known to most as a laxative that has a hyperosmotic effect, stimulating intestinal peristalsis. The drug has long established itself as a safe and effective medicine. Lactulose is one of the few drugs that is approved for use in pregnant women and children under 6 months of age with functional constipation. The prebiotic properties of lactulose were discovered in 1957. After research, it was found that it promotes the growth of beneficial bacteria, such as bifidobacteria and lactobacilli. In addition to being used as an effective weak and prebiotic agent, lactulose has been successfully used since 1966 for the treatment of hepatic encephalopathy. The mechanism of action of the drug is that it prevents the absorption of excess ammonia, which is formed in the large intestine, through the hydrolysis of protein and urea by the intestinal microflora. Lactulose, which has a wide range of effects on nitrogen metabolism by the intestinal microflora, affects not only ammonia, but also other bacterial toxins as a result of the studies that have demonstrated the reliable effectiveness of the drug, the world’s leading professional communities have included lactulose in their recommendations as the drug of choice for the treatment of patients with hepatic encephalopathy at any stage of the disease. The article presents current data on the effectiveness and safety of the use of lactulose in various diseases. In addition, attention is paid to such a concept as microbiota. Its functions and influence on the human body are described. 

Nanoparticles Functionalized with Venom-Derived Peptides and Toxins for Pharmaceutical Applications

2020 ◽  
Vol 21 (2) ◽  
pp. 97-109 ◽  
Author(s):  
Ana P. dos Santos ◽  
Tamara G. de Araújo ◽  
Gandhi Rádis-Baptista
Keyword(s):  
Oral Absorption ◽  
Current Data ◽  
Pharmacological Activities ◽  
Venom Peptides ◽  
Essential Components ◽  
Wide Range ◽  
Different Types ◽  
Direct Use ◽  
Animal Venoms ◽  
Pharmaceutical Biotechnology

Venom-derived peptides display diverse biological and pharmacological activities, making them useful in drug discovery platforms and for a wide range of applications in medicine and pharmaceutical biotechnology. Due to their target specificities, venom peptides have the potential to be developed into biopharmaceuticals to treat various health conditions such as diabetes mellitus, hypertension, and chronic pain. Despite the high potential for drug development, several limitations preclude the direct use of peptides as therapeutics and hamper the process of converting venom peptides into pharmaceuticals. These limitations include, for instance, chemical instability, poor oral absorption, short halflife, and off-target cytotoxicity. One strategy to overcome these disadvantages relies on the formulation of bioactive peptides with nanocarriers. A range of biocompatible materials are now available that can serve as nanocarriers and can improve the bioavailability of therapeutic and venom-derived peptides for clinical and diagnostic application. Examples of isolated venom peptides and crude animal venoms that have been encapsulated and formulated with different types of nanomaterials with promising results are increasingly reported. Based on the current data, a wealth of information can be collected regarding the utilization of nanocarriers to encapsulate venom peptides and render them bioavailable for pharmaceutical use. Overall, nanomaterials arise as essential components in the preparation of biopharmaceuticals that are based on biological and pharmacological active venom-derived peptides.

scholarly journals Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in continuous culture

1972 ◽  
Vol 127 (1) ◽  
pp. 87-96 ◽  
Author(s):  
P. G. Bolton ◽  
A. C. R. Dean
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Continuous Culture ◽  
Activity Profile ◽  
Glucose Effect ◽  
Ph Values ◽  
Nitrophenyl Phosphate ◽  
Wide Range ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

1. Phosphatase synthesis was studied in Klebsiella aerogenes grown in a wide range of continuous-culture systems. 2. Maximum acid phosphatase synthesis was associated with nutrient-limited, particularly carbohydrate-limited, growth at a relatively low rate, glucose-limited cells exhibiting the highest activity. Compared with glucose as the carbon-limiting growth material, other sugars not only altered the activity but also changed the pH–activity profile of the enzyme(s). 3. The affinity of the acid phosphatase in glucose-limited cells towards p-nitrophenyl phosphate (Km 0.25–0.43mm) was similar to that of staphylococcal acid phosphatase but was ten times greater than that of the Escherichia coli enzyme. 4. PO43−-limitation derepressed alkaline phosphatase synthesis but the amounts of activity were largely independent of the carbon source used for growth. 5. The enzymes were further differentiated by the effect of adding inhibitors (F−, PO43−) and sugars to the reaction mixture during the assays. In particular, it was shown that adding glucose, but not other sugars, stimulated the rate of hydrolysis of p-nitrophenyl phosphate by the acid phosphatase in carbohydrate-limited cells at low pH values (<4.6) but inhibited it at high pH values (>4.6). Alkaline phosphatase activity was unaffected. 6. The function of phosphatases in general is discussed and possible mechanisms for the glucose effect are outlined.

scholarly journals Nesting of Great Cormorants (Phalacrocorax carbo) on man-made structures in Ukraine

2016 ◽  
Vol 24 (2) ◽  
Author(s):  
A. I. Sidorenko ◽  
V. D. Siokhin
Keyword(s):  
National Level ◽  
Field Work ◽  
Current Data ◽  
Great Cormorant ◽  
Phalacrocorax Carbo ◽  
Breeding Sites ◽  
Breeding Territory ◽  
Wide Range ◽  
Maintenance Work ◽  
Great Cormorants

In Ukraine the Great Cormorant (Phalacrocorax carbo Linnaeus, 1758) uses a rather wide range of habitats for nesting: islands, trees and shrubs, reedbeds and a variety of man-made structures. In general, the strategy of nesting on man-made structures is uncommon both in Ukraine and Europe, and Cormorantsdo this only in the absence of other sites suitable for nesting. Special research onCormorant colonies on technogenic constructions was carried out during the field expeditions by the Research Institute of Biodiversity of Terrestrial and Aquatic Ecosystems of Ukraine in 2002–2003 and 2012–2016. Besides this, we used retrospective and current data from the literature and Internet resources. Most of the field work was carried out by making surveys by boat and on foot. As a result, we found 8 Cormorant colonies on technogenic constructions in Ukraine: gas platforms in the Sea of Azov (near the village Strilkove, Henichesk district of Kherson region); sunken ships – targets for bombing training near the Arabat Spit (these are also known as «ship islands»); electricity pylons of the high-voltage Enerhodar Dnipro Power Line where it crosses the Kakhovka Reservoir; the dock in Yahorlyk Bayk, used in the past as a target for bombing training bombing; artificial island-platforms on Lake Chernine (Kinburn Peninsula); an artificial island on the Sasyk Lagoon (Odessa region); artificial islands, made as navigation markers on the Kremenchuk and Kiev reservoirs. The study found that in most cases the accompanying species was the CaspianGull (Larus cachinnans Pallas, 1811), which actively destroys the Cormorants’ nests and eats their eggs and chicks. The number of nests in the colonies varied greatly (5–30 nests on the navigation marker islands and ca. 2 000–2 300 on the «ship-islands» and gas platforms). This is due, primarily, to the area of the breeding territory. The research found that fierce territorial competition was observed in most of the colonies both with Caspian Gulls and between Cormorants. In addition, we observed anthropogenic interference in the colonies by fishermen and workers conducting routine maintenance work (as happened in the case of electricity pylons and gas platforms). The benefit of this study is that it is the first research in Ukraine conducted at national level onthis type of nesting by Cormorants. Moreover, the study examines the history of emergence of these nesting territories and population dynamics of the Great Cormorant from the time of initial settlement of the breeding sites till the present.

Molybdenum-Mediated One-Pot Synthesis of Pyrazoles from Isoxazoles

Synthesis ◽  
2019 ◽  
Vol 51 (20) ◽  
pp. 3796-3804 ◽  
Author(s):  
Marine Rey ◽  
Stéphane Beaumont
Keyword(s):  
Bond Cleavage ◽  
Direct Synthesis ◽  
Molybdenum Complex ◽  
One Pot ◽  
One Pot Synthesis ◽  
Wide Range ◽  
Hydrolysis Of ◽  
Substituted Pyrazoles

A one-pot approach for the direct synthesis of substituted pyrazoles from isoxazoles is reported. The process involves isoxazole N–O bond cleavage mediated by a molybdenum complex, in situ hydrolysis of the resulting β-amino enone to the corresponding 1,3-diketone, followed by pyrazole formation in the presence of hydrazine or substituted hydrazine. Good to excellent yields and regioselectivities are obtained with nonsymmetric isoxazoles. By using readily available starting materials, a wide range of substituted pyrazoles may be synthesized by this method.

Comparison of In Situ Current Measurement Methods, the Accuracy Achieved in the Field and Recommendations for Engineering Design Applications

2018 ◽  
Author(s):  
Gregory Bush ◽  
Carey Nolan
Keyword(s):  
Engineering Design ◽  
Current Data ◽  
Measurement Methods ◽  
Significant Error ◽  
Acoustic Backscatter ◽  
Poor Agreement ◽  
Significant Cost ◽  
Wide Range ◽  
Comparison Data

Here we present a wealth of comparison data from acoustic backscatter current meters against current meters that use an active acoustic path (the RPS CM04). The data cover a wide range of environmental conditions. Very poor agreement between current meters frequently occurs, presenting as both bias and noise. The causes of the differences remain unproven, although for acoustic profilers deployed in deep water, failure of the assumption of the same flow in all beams is a possible source of significant error. As a result of decade’s worth of comparisons plagued with unexplained differences, we have reduced confidence in the accuracy of current data collected by acoustic backscatter sensors. We therefore avoid (where practical) using these instruments when the application of the current data is for engineering design, where uncertainty in accuracy can have significant cost or risk consequences.

Enzyme-Mediated Self-Assembly of Highly Ordered Structures From Disordered Proteins

2008 ◽  
Author(s):  
Ahmad Athamneh ◽  
Justin Barone
Keyword(s):  
Self Assembly ◽  
Amyloid Fibrils ◽  
Prion Diseases ◽  
Wheat Gluten ◽  
Disordered Proteins ◽  
X Ray Diffraction ◽  
Ordered Structures ◽  
Wide Range ◽  
Trypsin Hydrolysis ◽  
Hydrolysis Of

Trypsin hydrolysis of wheat gluten produced glutamine-rich short peptides with a tendency to self-assemble into supermolecular structures extrinsic to native wheat gluten. Fourier transform infrared and X-ray diffraction data suggested that the new structures formed resembled that of cross-β amyloid fibrils found in some insect silk and implicated in prion diseases. The superstructures were about 10 μm in diameter with clear right-handed helical configuration and appeared to be bundles of smaller fibrils of about 63 nm in diameter. Results demonstrate the potential for utilizing cheap protein sources and natural mechanisms of protein self-assembly to design advanced nanomaterials that can provide a wide range of structural and chemical functionality.

scholarly journals Medicinal Cannabis and Synthetic Cannabinoid Use

Medicina ◽  
2020 ◽  
Vol 56 (9) ◽  
pp. 453
Author(s):  
Simona Pichini ◽  
Alfredo Fabrizio Lo Faro ◽  
Francesco Paolo Busardò ◽  
Raffaele Giorgetti
Keyword(s):  
Adverse Effects ◽  
Therapeutic Potential ◽  
Neurological Diseases ◽  
Synthetic Cannabinoids ◽  
Current Data ◽  
Special Issue ◽  
Biological Matrices ◽  
Wide Range ◽  
Medicinal Cannabis ◽  
Harmful Effects

Cannabis products have been used for centuries by humans for recreational and medical purposes. Resent research, proposed the promising therapeutic potential of cannabis and related cannabinoids for a wide range of medical conditions, including psychiatric and neurological diseases. This Special Issue presents the latest updates on medicinal cannabis and synthetic cannabinoids pharmacology, toxicology and new analytical methods to identify and quantify these compounds in conventional and non-conventional biological matrices. Moreover, it provides current data regarding their adverse effects, safety, application for medical purposes and their harmful effects.

scholarly journals RUNX1-ETO: Attacking the Epigenome for Genomic Instable Leukemia

2019 ◽  
Vol 20 (2) ◽  
pp. 350 ◽  
Author(s):  
Emiel van der Kouwe ◽  
Philipp Staber
Keyword(s):  
Fusion Protein ◽  
Target Genes ◽  
Underlying Mechanism ◽  
Current Data ◽  
Distal Enhancer ◽  
Dna Repair Mechanisms ◽  
Genome Wide ◽  
Wide Range ◽  
Repair Mechanisms ◽  
Comprehensive Picture

Oncogenic fusion protein RUNX1-ETO is the product of the t(8;21) translocation, responsible for the most common cytogenetic subtype of acute myeloid leukemia. RUNX1, a critical transcription factor in hematopoietic development, is fused with almost the entire ETO sequence with the ability to recruit a wide range of repressors. Past efforts in providing a comprehensive picture of the genome-wide localization and the target genes of RUNX1-ETO have been inconclusive in understanding the underlying mechanism by which it deregulates native RUNX1. In this review; we dissect the current data on the epigenetic impact of RUNX1 and RUNX1-ETO. Both share similarities however, in recent years, research focused on epigenetic factors to explain their differences. RUNX1-ETO impairs DNA repair mechanisms which compromises genomic stability and favors a mutator phenotype. Among an increasing pool of mutated factors, regulators of DNA methylation are frequently found in t(8;21) AML. Together with the alteration of both, histone markers and distal enhancer regulation, RUNX1-ETO might specifically disrupt normal chromatin structure. Epigenetic studies on the fusion protein uncovered new mechanisms contributing to leukemogenesis and hopefully will translate into clinical applications.

scholarly journals Researching the delivery of health and nutrition interventions for women and children in the context of armed conflict: lessons on research challenges and strategies from BRANCH Consortium case studies of Somalia, Mali, Pakistan and Afghanistan

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Michelle F. Gaffey ◽  
Anushka Ataullahjan ◽  
Jai K. Das ◽  
Shafiq Mirzazada ◽  
Moctar Tounkara ◽  
...  
Keyword(s):  
Decision Making ◽  
Data Collection ◽  
Case Studies ◽  
Nutrition Interventions ◽  
Health And Nutrition ◽  
Research Challenges ◽  
Conflict Settings ◽  
Wide Range ◽  
Women And Children

Abstract Background The BRANCH Consortium recently conducted 10 mixed-methods case studies to investigate the provision of health and nutrition interventions for women and children in conflict-affected countries, aiming to better understand the dominant influences on humanitarian health actors’ programmatic decision-making and how such actors surmount intervention delivery barriers. In this paper, the research challenges encountered and the mitigating strategies employed by the case study investigators in four of the BRANCH case study contexts are discussed: Somalia, Mali, Pakistan and Afghanistan. Discussion Many of the encountered research challenges were anticipated, with investigators adopting mitigation strategies in advance or early on, but others were unexpected, with implications for how studies were ultimately conducted and how well the original study aims were met. Insecurity was a fundamental challenge in all study contexts, with restricted geographical access and concerns for personal safety affecting sampling and data collection plans, and requiring reliance on digital communications, remote study management, and off-site team meetings wherever possible. The need to navigate complex local sociopolitical contexts required maximum reliance on local partners’ knowledge, expertise and networks, and this was facilitated by early engagement with a wide range of local study stakeholders. Severe lack of reliable quantitative data on intervention coverage affected the extent to which information from different sources could be triangulated or integrated to inform an understanding of the influences on humanitarian actors’ decision-making. Conclusion Strong local partners are essential to the success of any project, contributing not only technical and methodological capacity but also the insight needed to truly understand and interpret local dynamics for the wider study team and to navigate those dynamics to ensure study rigour and relevance. Maintaining realistic expectations of data that are typically available in conflict settings is also essential, while pushing for more resources and further methodological innovation to improve data collection in such settings. Finally, successful health research in the complex, dynamic and unpredictable contexts of conflict settings requires flexibility and adaptability of researchers, as well as sponsors and donors.

scholarly journals Electrophoretic and zymographic techniques for production monitoring of two lipase forms from Candida antarctica DSM 70725

2012 ◽  
Vol 66 (2) ◽  
pp. 201-206
Author(s):  
Aleksandra Dimitrijevic ◽  
Dusan Velickovic ◽  
Ratko Jankov ◽  
Nenad Milosavic
Keyword(s):  
Molecular Weight ◽  
Large Scale ◽  
Fermentation Broth ◽  
Candida Antarctica ◽  
Simple Method ◽  
Wide Range ◽  
Work Production ◽  
Lipase A ◽  
Dimeric Form ◽  
Hydrolysis Of

Yeast Candida antarctica produces two lipase forms, which are widely used as catalysts in variety of organic reactions, many of which are applied on a large scale. In this work, production of two forms of lipase from C. antarctica DSM 70725 (CAL A and CAL B) was monitored during seven days of cultivation in the optimal medium using different electrophoretic and zymographic techniques. According to electrophoresis after silver staining, C. antarctica lipase A (molecular mass 45 kDa) was produced starting from the second day of cultivation. C. antarctica lipase B (CAL B) was also produced starting from the second day, but protein was present in the fermentation broth predominantly as dimer (molecular weight 66 kDa), while presence of monomeric form of CAL B (molecular weight of 33 kDa) was observed starting from the fourth day of cultivation. Both types of zymograms (based on hydrolysis and synthesis reactions) were used for detection of lipase activity in the fermentation broth. C. antarctica lipase A showed activity only in hydrolytic zymogram, when ?-naphtyl butyrate was used as substrate. In the same zymogram, with ?-naphtyl acetate as substrate no CAL A activity was detected. Similarly, CAL A showed no activity in synthesis based zymograms towards oleic acid and octanol as substrates, indicating that CAL A is not active towards very short or long-chain substrates. As opposite of CAL A, both monomeric and dimeric form of CAL B were detected in the all zymograms, suggesting that CAL B is active towards wide range of substrates, regardless to the chain length. Thus, zymogram based on hydrolysis of ?-naphtyl butyrate represents a simple method for monitoring the production of two forms of lipase from C. antarctica, that greatly differ in their characteristics.

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