Nematicidal Activity of some Nanoemulsions of Monoterpenes on Tomato Root-Knot Nematodes (Meloidogyne javanica)

Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. Duddingtonia flagrans is a type of nematode-trapping fungi (NTF), which produces three-dimensional adhesive networks to trap nematodes. In this study, the pathogenicity and volatile organic compounds (VOCs) of the NTF D. flagrans against the plant root-knot nematode, Meloidogyne incognita, were investigated. The predatory process of D. flagrans trapping M. incognita was observed using scanning electron microscopy. Gas chromatography-mass spectrometry analysis of the VOCs from D. flagrans led to the identification of 52 metabolites, of which 11 main compounds were tested individually for their activity against M. incognita. Three compounds, cyclohexanamine, cyclohexanone, and cyclohexanol, were toxic to M. incognita. Furthermore, these three VOCs inhibited egg hatching of M. incognita. Cyclohexanamine showed the highest nematicidal activity, which can cause 97.93% mortality of M. incognita at 8.71 µM within 12 h. The number of hatched juveniles per egg mass after 3 days was just 8.44 when treated with 26.14 µM cyclohexanamine. This study is the first to demonstrate the nematicidal activity of VOCs produced by D. flagrans against M. incognita, which indicates that D. flagrans has the potential to biocontrol plant root-knot nematodes.

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Biocontrol properties of some agricultural waste extracts on three nematode species in in vitro and in vivo conditions

Nematology ◽

10.1163/15685411-00003258 ◽

2019 ◽

Vol 21 (8) ◽

pp. 837-846

Author(s):

Ali Roshan-Bakhsh ◽

Ebrahim Pourjam ◽

Mahdi Ayyari ◽

Majid Pedram

Keyword(s):

Faba Bean ◽

Agricultural Waste ◽

Ethanolic Extract ◽

Nematode Species ◽

Nematicidal Activity ◽

Leaf Extracts ◽

Tomato Root ◽

Infection Parameters

Summary Extracts of nine agricultural wastes prepared with five different solvents were assessed for their potential nematicidal activity against three nematode species, Aphelenchus avenae, Meloidogyne incognita and Pratylenchus neglectus, in in vitro condition. The 50% v/v hydro-ethanolic extracts showed the highest performance for two tested plant wastes of cabbage leaves and faba bean pods. These two extracts were tested on nematodes in three different concentrations. The highest in vitro nematistatic activity was recorded for 3000 and 1500 ppm of cabbage leaf extracts by 100% paralysis of all three nematode species after 48 h, and the highest nematicidal activity was recorded for the above-mentioned extract by 25-100% mortality depending on nematode species and extract concentration. A 14-94% mortality was recorded for all three species of nematodes after treatment with faba bean pod hydro-ethanolic extract in in vitro conditions. Hatching inhibition and repellent activity of cabbage leaf and faba bean pod extracts were observed in P. neglectus and M. incognita. In vivo assays confirmed the in vitro results when both of the extracts showed moderate to high inhibition of nematode population development and nematode infection parameters on tomato root system in pot experiments.

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Suppressive effects of composts against the root-knot nematode Meloidogyne javanica on tomato

Nematology ◽

10.1163/156854102321122502 ◽

2002 ◽

Vol 4 (8) ◽

pp. 891-898 ◽

Cited By ~ 40

Author(s):

Yuji Oka ◽

Uri Yermiyahu

Keyword(s):

Water Extract ◽

Meloidogyne Javanica ◽

Suppressive Effect ◽

Cattle Manure ◽

Nematicidal Activity ◽

Root Knot Nematode ◽

Grape Marc ◽

Manure Compost ◽

Cattle Manure Compost

AbstractSuppressive effects of two composts, from cattle manure and grape marc, on the root-knot nematode Meloidogyne javanica were tested in pot and in vitro experiments. No root galls were found on tomato roots grown in soils containing 10 or 25% (v/v) cattle manure compost, and very few on those grown in 50% grape marc compost. Significant reductions in galling index were also found on tomato plants grown in soils containing lower concentrations of this compost. Chemical analysis of the composts and leachates from the soils showed that the cattle manure compost had higher electrical conductivity (EC) and higher concentrations of nitrogen, especially N–NH4, than the grape marc compost. Water extract of the cattle manure compost showed high nematicidal activity to the nematode juveniles and less activity toward the eggs in vitro. Water extract of the grape marc compost showed weaker nematicidal activity to the juveniles and eggs. Washing composted soils with excess water before nematode inoculation and tomato planting led to better plant growth, but the nematode-suppressive effect was decreased. These results suggest that high nitrogen concentrations, especially N–NH4, and high EC values contribute to the nematode suppressiveness of the composts.

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Effective concentration and range of activity of abamectin as seed treatment against root-knot nematodes in tomato under glasshouse conditions

Nematology ◽

10.1163/156854109x433371 ◽

2009 ◽

Vol 11 (6) ◽

pp. 909-915 ◽

Cited By ~ 5

Author(s):

J. Alfonso Cabrera ◽

Sebastian Kiewnick ◽

Christoph Grimm ◽

Abd el-Fattah A. Dababat ◽

Richard A. Sikora

Keyword(s):

Root Growth ◽

Active Ingredient ◽

Meloidogyne Incognita ◽

Growth And Development ◽

Seed Treatment ◽

Effective Concentration ◽

Tomato Root ◽

Root Knot Nematodes ◽

Root Growth And Development ◽

Meloidogyne Species

Abstract The objectives of this study were to determine the efficacy, range of activity and effective concentrations (EC50 and EC80) of abamectin as a seed treatment of tomato against Meloidogyne incognita, M. arenaria and M. javanica. The study revealed that abamectin seed treatment at concentrations ranging between 0.3 and 1 mg a.s. seed−1 is highly effective against the three species of root-knot nematodes, retaining its efficacy in the soil for 8 weeks. The highest EC50 found was reached at 0.2 mg a.s. seed−1. The highest EC80 for the number of egg masses per g root in the three Meloidogyne species was attained at 0.51 mg a.s. seed−1. Abamectin as a seed treatment is promising for the control of root-knot nematodes in the field since only low amounts of active ingredient are required to give adequate protection in the most sensitive stages of tomato root growth and development.

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Nematicidal activity of Haplophyllum tuberculatum and Plectranthus cylindraceus oils against Meloidogyne javanica

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2008.05.005 ◽

2008 ◽

Vol 36 (9) ◽

pp. 679-683 ◽

Cited By ~ 18

Author(s):

Anthony K. Onifade ◽

Majekodunmi O. Fatope ◽

Michael L. Deadman ◽

Salma M.Z. Al-Kindy

Keyword(s):

Meloidogyne Javanica ◽

Nematicidal Activity ◽

Haplophyllum Tuberculatum

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THE EFFECT OF ROOT-KNOT NEMATODES ON THE YIELD OF PROCESSING TOMATOES

Canadian Journal of Plant Science ◽

10.4141/cjps64-051 ◽

1964 ◽

Vol 44 (3) ◽

pp. 265-267 ◽

Cited By ~ 2

Author(s):

R. M. Sayre ◽

T. K. Toyama

Keyword(s):

Field Tests ◽

Meloidogyne Javanica ◽

Root Knot Nematodes ◽

Tomato Variety ◽

Processing Tomato ◽

Processing Tomatoes

In field tests, yields of the processing tomato variety Campbell 135 were not significantly reduced by slight to moderate infestations of the root-knot nematodes Meloidogyne javanica (Treub, 1885) Chitwood, 1949 and M. hapla Chitwood, 1949.

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Efficacy of neem (Azadirachta indica) formulations on biology of root-knot nematodes (Meloidogyne javanica) on tomato

Crop Protection ◽

10.1016/j.cropro.2007.04.006 ◽

2008 ◽

Vol 27 (1) ◽

pp. 36-43 ◽

Cited By ~ 24

Author(s):

N. Javed ◽

S.R. Gowen ◽

S.A. El-Hassan ◽

M. Inam-ul-Haq ◽

F. Shahina ◽

...

Keyword(s):

Azadirachta Indica ◽

Meloidogyne Javanica ◽

Root Knot Nematodes

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Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

Frontiers in Microbiology ◽

10.3389/fmicb.2021.726504 ◽

2021 ◽

Vol 12 ◽

Author(s):

Van Thi Nguyen ◽

Nan Hee Yu ◽

Yookyung Lee ◽

In Min Hwang ◽

Hung Xuan Bui ◽

...

Keyword(s):

Culture Filtrate ◽

Cyclopiazonic Acid ◽

Nematicidal Activity ◽

Broth Culture ◽

Root Knot Nematodes ◽

Potato Dextrose Broth ◽

Tomato Roots ◽

Penicillium Commune ◽

Burman Design ◽

Plackett Burman Design

Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of Penicillium commune, which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity. CPA was isolated from the culture filtrate of P. commune KACC 45973. CPA killed the second-stage juveniles of M. incognita, M. hapla, and M. arearia with EC50–3 days 4.50, 18.82, and 60.51 μg mL–1, respectively. CPA also significantly inhibited egg hatch of M. incognita and M. hapla after a total of 28 days of treatment with the concentrations > 25 μg mL–1. The enhancement of CPA production by P. commune KACC 45973 was explored using an optimized medium based on Plackett–Burman design (PBD) and central composite design (CCD). The highest CPA production (381.48 μg mL–1) was obtained from the optimized medium, exhibiting an increase of 7.88 times when compared with that from potato dextrose broth culture. Application of the wettable power-type formulation of the ethyl acetate extract of the culture filtrate of KACC 45973 reduced gall formation and nematode populations in tomato roots and soils under greenhouse conditions. These results suggest that CPA produced by P. commune KACC 45973 can be used as either a biochemical nematicide or a lead molecule for developing chemical nematicides to control root-knot nematodes.

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Community Analysis of Nematodes Associated with Banana, Identification of root knot nematode and Evaluation the Susceptibility of Some Cultivars to Infection

10.21203/rs.3.rs-740928/v1 ◽

2021 ◽

Author(s):

Radwa G. Mostafa ◽

Aida M. El-Zawahry ◽

Ashraf E. M. Khalil ◽

Ameer E. Elfarash ◽

Ali D. A. Allam

Keyword(s):

Meloidogyne Javanica ◽

Nematode Species ◽

Common Species ◽

Molecular Techniques ◽

Control Measures ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Plant Parasitic

Abstract Background Plant-parasitic nematodes are extremely dangerous pests in a variety of economically important crops. The purpose of this study was a survey of all nematode species existing in banana from three sites in Assiut Governorate, Egypt and to characterize the most common species by morphological, morphometric and molecular techniques (PCR with species-specific primers). Then, study of resistance or sensitivity of some banana cultivars to root-knot nematodes.Methods and Results Four nematodes, Meloidogyne, Rotylenchulus reniformis, Helicotylenchus and Pratylenchus were isolated and identified from soil and root samples collected from banana plants. Most frequently occurring of plant parasitic nematode species in banana was Meloidogyne. Former research found differences in species and in resistance to root-knot nematodes among the examined plant cultivars. Identification of Root-knot nematodes by Characterize of morphometric, molecularly, morphological isolate of Meloidogyne related to banana plants. The results revealed that the identified nematode species, Meloidogyne javanica, is the most common plant-parasitic nematodes in all locations. Data on the susceptibility of the tested banana cultivars to M. javanica revealed that Grand Naine was highly susceptible (HS) however, Magraby was susceptible (S) but Williams and Hindi cultivars were moderately resistant (MR).Conclusions we concluded that a survey revealed the significant prevalence of Meloidogyne javanica, the most important nematodes on banana in Assiut. The morphometric, morphological, and molecular identification were harmonic with one another. In addition to the host response of certain banana cultivars, to M. javanica that resistance is of significance and can be helpful to incorporate through planning control measures for root- knot nematodes.

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Meloidogyne Species Associated with Weeds in Rio Grande do Sul

Planta Daninha ◽

10.1590/s0100-83582019370100095 ◽

2019 ◽

Vol 37 ◽

Cited By ~ 1

Author(s):

C. BELLÉ ◽

T.E. KASPARY ◽

R.R. BALARDIN ◽

R.F. RAMOS ◽

Z.I. ANTONIOLLI

Keyword(s):

Host Plants ◽

Management Practices ◽

Rio Grande ◽

Meloidogyne Javanica ◽

Rio Grande Do Sul ◽

Weed Species ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Meloidogyne Spp ◽

Meloidogyne Species

ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was detected in 15.6% of the samples. M. incognita Est. I2 (Rm: 1.0, 1.1), M. ethiopica Est. E3 (Rm: 0.9, 1.15, 1.30), M. enterolobii Est. M2 (Rm: 0.7, 0.75, 0.9, 0.95) and M. hapla Est. H1 (Rm: 1.17) in 13.3%, 8.9%, 6.7% and 2.2% of the samples, respectively. Therefore, knowledge of the range of host plants to different species of the root-knot nematode can positively contribute to the adoption of management practices that allow the reduction of their populations in the soil.

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