scholarly journals Exploration of coliform diversity in drinking water resources by culture-independent approaches

2020 ◽  
Vol 10 (3) ◽  
pp. 580-588
Author(s):  
A. Shiva Shanker ◽  
Praveen Kumar Vootla ◽  
Pavan Kumar Pindi
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Total Coliform ◽  
Gene Clone ◽  
Rrna Gene ◽  
Waterborne Diseases ◽  
Limited Capacity ◽  
Drinking Water Resources ◽  
Culture Independent ◽  
Public Health Protection

Abstract The coliform group has been widely used as an indicator of water quality and has historically led to a public health protection concept. Presence of pathogens in drinking water may raise several health problems in humans from mild illnesses to serious waterborne diseases. In spite of several measures taken, water quality is always a pertinent issue prevailing in diverse water systems. So far, coliform contamination and diversity could not be adequately explored as traditionally used culture-dependent methods have a limited capacity to characterize microbiota from their respective sources. The study was designed for assessment of microbial diversity by culture-independent approaches placing emphasis on exploring the total coliform diversity in two drinking water reservoirs, Raman Pahad and Koilsagar of Mahabubnagar district, Telangana, India. Principal analysis based on 16S rRNA gene clone libraries revealed that Raman Pahad library clones belonged to genus Enterobacter (41.5%), followed by Citrobacter (25.03%), Klebsiella (17.86%), Escherichia (12.20%), and the least being Hafnia (3.39%). The clones in Koilsagar belonged to genus Enterobacter (46.42%) as the most predominant, followed by Citrobacter (32.14%) and Escherichia (21.42%). Comparatively, Enterobacter was observed to be the most predominant (representing 50%) of the total clones in both reservoirs.

scholarly journals Analysis of Physiochemical Parameters to Evaluate the Drinking Water Quality in the State of Perak, Malaysia

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
N. Rahmanian ◽  
Siti Hajar Bt Ali ◽  
M. Homayoonfard ◽  
N. J. Ali ◽  
M. Rehan ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Quality Standard ◽  
The State ◽  
Drinking Water Quality ◽  
World Health ◽  
Physiochemical Parameters ◽  
Health Organization ◽  
Public Health Protection ◽  
Physical And Chemical

The drinking water quality was investigated in suspected parts of Perak state, Malaysia, to ensure the continuous supply of clean and safe drinking water for the public health protection. In this regard, a detailed physical and chemical analysis of drinking water samples was carried out in different residential and commercial areas of the state. A number of parameters such as pH, turbidity, conductivity, total suspended solids (TSS), total dissolved solids (TDS), and heavy metals such as Cu, Zn, Mg, Fe, Cd, Pb, Cr, As, Hg, and Sn were analysed for each water sample collected during winter and summer periods. The obtained values of each parameter were compared with the standard values set by the World Health Organization (WHO) and local standards such as National Drinking Water Quality Standard (NDWQS). The values of each parameter were found to be within the safe limits set by the WHO and NDWQS. Overall, the water from all the locations was found to be safe as drinking water. However, it is also important to investigate other potential water contaminations such as chemicals and microbial and radiological materials for a longer period of time, including human body fluids, in order to assess the overall water quality of Perak state.

scholarly journals Physiochemical and Microbiological Analysis of Drinking Water in Chattogram City, Bangladesh

2020 ◽  
pp. 42-50
Author(s):  
Kanij Fatema Nishan ◽  
Nilufa Yeasmin ◽  
Urmi Rani Devi ◽  
Sumiya Akter ◽  
Md. Abu Bakar ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Total Coliform ◽  
Drinking Water Quality ◽  
Sampling Location ◽  
Microbiological Analysis ◽  
Alternative Source ◽  
Safe Alternative ◽  
Total Dissolved Solid

Chattogram is the second most populated city in Bangladesh. This port city faces a serious threat mainly due to the lack of safe drinking water. This study was conducted for determining drinking water quality of groundwater sources in Chattogram city. The study was performed in the BCSIR laboratory, Chattogram. It was carried out for a period of six months from 1st July, 2018 to 31th December, 2018. Total six water samples were collected from three different locations (Baluchora, C&B colony and Khulshi area). Each sampling location consists of two separate sampling points. Physicochemical parameters of the collected samples like Temperature, pH, Electrical Conductivity (EC), Total dissolved solid (TDS), Hardness, Turbidity and concentration of Cl, As, Mn, Fe, Pb, Cr and Cd were examined. Microbial parameters like Total Coliform (TC) were also measured. All the analyzed parameters compared with BSTI and WHO drinking water quality standards to understand the overall ground water quality status of the study area. The results reveal that water samples in almost all locations were contaminated with microbial contamination and that the range of physico-chemical parameters was not adequate for consumption. Preliminary treatments like boiling, filtering etc are required before using groundwater for drinking and the necessary measures must be taken for a safe alternative source of drinking water.

scholarly journals Characterizing a Newly Designed Steel-Wool-Based Household Filter for Safe Drinking Water Provision: Hydraulic Conductivity and Efficiency for Pathogen Removal

Processes ◽  
2019 ◽  
Vol 7 (12) ◽  
pp. 966 ◽  
Author(s):  
Raoul Tepong-Tsindé ◽  
Arnaud Igor Ndé-Tchoupé ◽  
Chicgoua Noubactep ◽  
Achille Nassi ◽  
Hans Ruppert
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Hydraulic Conductivity ◽  
Ph Value ◽  
Iron Concentration ◽  
Progressive Increase ◽  
Total Coliform ◽  
Drinking Water Quality ◽  
Safe Drinking Water ◽  
Steel Wool

This study characterizes the decrease of the hydraulic conductivity (permeability loss) of a metallic iron-based household water filter (Fe0 filter) for a duration of 12 months. A commercial steel wool (SW) is used as Fe0 source. The Fe0 unit containing 300 g of SW was sandwiched between two conventional biological sand filters (BSFs). The working solution was slightly turbid natural well water polluted with pathogens (total coliform = 1950 UFC mL−1) and contaminated with nitrate ([NO3−] = 24.0 mg L−1). The system was monitored twice per month for pH value, removal of nitrate, coliforms, and turbidity, the iron concentration, as well as the permeability loss. Results revealed a quantitative removal of coliform (>99%), nitrate (>99%) and turbidity (>96%). The whole column effluent depicted drinking water quality. The permeability loss after one year of operation was about 40%, and the filter was still producing 200 L of drinking water per day at a flow velocity of 12.5 L h−1. A progressive increase of the effluent pH value was also recorded from about 5.0 (influent) to 8.4 at the end of the experiment. The effluent iron concentration was constantly lower than 0.2 mg L−1, which is within the drinking-water quality standards. This study presents an affordable design that can be one-to-one translated into the real world to accelerate the achievement of the UN Sustainable Development Goals for safe drinking water.

scholarly journals Enumeration of Total and Faecal Coliform Bacteria in Drinking Water of Khairpur City

1970 ◽  
Vol 24 (2) ◽  
pp. 163-165 ◽  
Author(s):  
Abdul Hussain Shar ◽  
Yasmeen F Kazi ◽  
Miandad Zardari ◽  
Irshad Hussain Soomro
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Faecal Coliform ◽  
World Health ◽  
Source Distribution ◽  
Coliform Bacteria ◽  
Residual Chlorine ◽  
Health Organization

Total coliform (TC) and faecal coliform (FC) bacteria were analyzed in drinking water of Khairpur city. Ninty samples were collected from main reservoir (source), distribution line and consumer taps. pH and residual chlorine of water samples were also determined. For bacteriological analysis inductively membrane filtration (MF) method was used for total coliform (TC) as well as faecal (FC) coliform bacteria. All samples were found contaminated with total coliform (TC) and faecal coliform (FC) and the counts were higher than the maximum microbial contaminant level (MMCL) established by World Health Organization (WHO). It was observed that pH was within the limits of WHO standard (6.5-8.5). The residual chlorine was not detected in any sample of drinking water. Bacteriologically the water quality of the drinking water is unsatisfactory.Keywords: Coliform, Escherichia coli, Water quality, Contamination, SanitationDOI: http://dx.doi.org/10.3329/bjm.v24i2.1266

scholarly journals Escherichia coli (E. coli) as an Indicator of Fecal Contamination in Water: A Review

2019 ◽  
Author(s):  
Farhan Mohammad Khan ◽  
Rajiv Gupta
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Coliform ◽  
Fecal Contamination ◽  
Waterborne Diseases ◽  
Fecal Matter ◽  
E Coli ◽  
Detection Techniques ◽  
Pollution Indicator

Escherichia coli or E. coli is a member of the fecal coliform group and is a more specific indicator of fecal contamination than other fecal coliform species, its presence indicate possibly presence of harmful bacteria which will cause diseases and it also suggests the extent as well as the nature of the contaminants. E. coli bacteria able to survive in water for 4 – 12 weeks and at present, it appears as an indicator to provide the accurate bacterial contamination of fecal matter in drinking water, because of the availability of simple, affordable, fast, sensitive and exact detection techniques. According to the laboratory experiment based techniques, 24 - 48 hours are required for the bacterial concentration to be reported. So, there is a necessity for continuous monitoring. Techniques for detection of many pathogenic bacterial strains are not yet available, sometimes days to weeks are required to get the results. To overcome the difficulties, expensive and time-consuming techniques are required to detect, count and identify the presence of specific bacterial strain. Public health relies on online monitoring of water quality that depends majorly on examination of fecal indicator bacteria, thus protection of health requires fecal pollution indicator so that it is not required to analyze drinking water to overcome the problems associated with waterborne diseases. This paper will brief the classification, sources, survival of E. coli bacteria and its correlation with basic water quality parameters in water sources.```

scholarly journals Household stored water quality in an intermittent water supply network in Panama

2020 ◽  
Vol 10 (2) ◽  
pp. 298-308
Author(s):  
Carlos I. Gonzalez ◽  
John Erickson ◽  
Karina A. Chavarría ◽  
Kara L. Nelson ◽  
Amador Goodridge
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Supply ◽  
Water Storage ◽  
Total Coliform ◽  
Drinking Water Supply ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Probable Number ◽  
Total Coliform Bacteria

Abstract Safe water storage is critical to preserve water quality, especially when intermittent piped drinking water supply creates a need for household storage. This study characterized household storage practices and stored water quality in 94 households (N = 94) among four peri-urban neighborhoods in Arraiján, Panama with varying degrees of supply intermittency. We found that 18 (19.1%) households stored drinking water in unsafe containers. Forty-four (47%) samples of household stored drinking water had residual chlorine levels <0.2 mg/L. While 33 (35.1%) samples were positive for total coliform bacteria, only 23 (24.4%) had >10 most probable number (MPN)/100 mL total coliform bacteria. Eight (44%) samples were positive for Escherichia coli, whereas only one (1.3%) sample from the safe containers was positive. Twenty-nine (30.9%) samples had >500 MPN/mL heterotrophic plate count bacteria. These findings suggest that longer supply interruptions were associated with longer storage times and lower chlorine residual, which were associated with higher concentrations of indicator bacteria. This is one of the first studies in the Central-American region to show an association between the lack of turnover (replacement with fresh water) and greater contamination during household water storage. Thus, when drinking water supply is not completely continuous and household storage is required, decreasing the time between supply periods can facilitate safer water storage. Public awareness and education are also recommended to increase hygiene practices during water collection and storage.

scholarly journals Low Impact Development (LID) as an effort to achieve a sustainable urban drainage system (SUDS). Case Study: Left Side Of Garang River Segment VI Semarang

2019 ◽  
Vol 125 ◽  
pp. 07017
Author(s):  
Anik Sarminingsih ◽  
Ganjar Samudro ◽  
Aisyatul Mas’adah
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Low Impact Development ◽  
Drainage System ◽  
Total Coliform ◽  
Urban Drainage ◽  
Raw Water ◽  
Industrial Estates ◽  
Standard Quality

The Garang River functions as a flood controller and a source of raw water for drinking water. This river is divided into seven segments, and in the downstream segment VI there is intake of raw water for drinking water. The land use is dominated by built-in land, in the form of dense settlements and industrial estates. This study aims to evaluate the condition of the drainage system, both in terms of runoff and water quality, and apply the concept of LID to achieve a sustainable drainage system. The method used is the SWMM. Evaluations were carried out in three outlets. In existing conditions, channel capacity in general is still sufficient, while water quality does not meet effluent standard quality for observed parameters including TSS, BOD, COD, oil & fat parameters and Total coliform. The LID concept that are suitable are Bio retention cell, Permeable Pavement and Rooftop disconnection.The use of this type of LID is significant enough to reduce runoff by 77% at outlet 1, 98% at outlet 2 and 73% at outlet 3 and decrease in some parameters of water quality by 77% to 100%.

PSIX-8 Drinking water acidification and chlorination improves microbiological water quality without detrimental effects on performance or hazard side-effects in bulls fed high-concentrate diets

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 438-439
Author(s):  
Marçal Verdú ◽  
Sonia Marti ◽  
Joan Riera ◽  
Carles Medinya ◽  
Jordi Cucurull ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Side Effects ◽  
Water Consumption ◽  
Total Coliform ◽  
Feed Consumption ◽  
Apparent Digestibility ◽  
Coliform Count ◽  
Microbiological Water Quality ◽  
Holstein Bulls

Abstract The aim of this study was to evaluate the effect of drinking water disinfection (chlorination) and conditioning (acidification) on microbiological water quality, water and feed consumption, total tract apparent digestibility and its potential hazard effects on animal health in Holstein bulls fed high-concentrate diets. Twenty-four Holstein bulls (221 ± 20.9 kg of initial BW, and 184 ± 9.9 d of age) were housed individually and fed ad libitum. Animals were assigned to 4 treatments with a 2 x 2 factorial design: drinking water conditioning (with or without acidification) and disinfection (absence or chlorination). Every 28 d, water samples from the tank and drinker were collected to analyze pH, residual chlorine and microbial load. Water consumption was recorded daily, and every 2 wks feed consumption and BW were recorded. At d 0, 14, 98 and 196 blood samples were collected to hematology and clinical chemistry analysis. At d 42 and 147 total tract apparent digestibility was estimated. Data were analyzed with a mixed effects model. In the tank, acidification increased residual free chlorine (0.58 vs. 0.33 ± 0.081 ppm, interaction, P = 0.10) and chlorination reduced (P < 0.01) total coliform count (0.8 vs. 392.7 ± 0.30 CFU / 100 ml) and Clostridium perfringens count (0.0 vs. 9.0 ± 0.13 CFU / 100 ml). In the drinkers, chlorination only tended (P = 0.10) to decrease total coliform count (967.5 vs. 994.7 ± 0.01 CFU / 100 ml). Treatments did not affect daily water consumption, total DMI nor blood parameters. At the end of the study, chlorination tended (P = 0.07) to improve starch total apparent digestibility (98.2 vs 97.7 ± 0.28 %). In fattening bulls’, the conditioning (acidification) and disinfection (chlorination) of drinking water improved its microbiological quality without detrimental effects on water and feed consumption, ruminal digestibility or hazard side-effects.

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