Distribution of Enteric Viruses and Microorganisms in Long-Term Sewage-Treated Soil

1983 ◽  
Vol 15 (5) ◽  
pp. 129-135 ◽  
Author(s):  
Z Filip ◽  
K Seidel ◽  
H Dizer
Keyword(s):  
Soil Bacteria ◽  
Pathogenic Bacteria ◽  
Sewage Treatment ◽  
Anaerobic Bacteria ◽  
Enteric Viruses ◽  
Sewage Irrigation ◽  
Groundwater Samples ◽  
West Berlin ◽  
Aerobic Soil

To determine whether long-term sewage treatment can take place without a pollution risk for soil and groundwater, samples from sewage irrigation fields in West Berlin which have been in use since about 1890 were analyzed for enteric viruses and microorganisms. Enteric viruses were detected in only seven samples from a total number of eighty seven taken from different soil depths. With only one exception, no viruses were found below 60 cm. No viruses were detected in groundwater samples. Long-term sewage irrigation did not result in significant changes in the colony courts of aerobic soil bacteria, but the counts of anaerobic bacteria and actinomycetes were slightly elevated and those of microscopic fungi slightly decreased. Potentially pathogenic bacteria were not detected in soil below 90 cm.

scholarly journals Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shuyi Wu ◽  
Jianmeng Xu ◽  
Leiyan Zou ◽  
Shulu Luo ◽  
Run Yao ◽  
...  
Keyword(s):  
Dental Implant ◽  
Pathogenic Bacteria ◽  
Titanium Surface ◽  
Polymeric Coating ◽  
Antibacterial Efficacy ◽  
Polymeric Coatings ◽  
Implant Infection ◽  
Titanium Surfaces

AbstractPeri-implant infection is one of the biggest threats to the success of dental implant. Existing coatings on titanium surfaces exhibit rapid decrease in antibacterial efficacy, which is difficult to promisingly prevent peri-implant infection. Herein, we report an N-halamine polymeric coating on titanium surface that simultaneously has long-lasting renewable antibacterial efficacy with good stability and biocompatibility. Our coating is powerfully biocidal against both main pathogenic bacteria of peri-implant infection and complex bacteria from peri-implantitis patients. More importantly, its antibacterial efficacy can persist for a long term (e.g., 12~16 weeks) in vitro, in animal model, and even in human oral cavity, which generally covers the whole formation process of osseointegrated interface. Furthermore, after consumption, it can regain its antibacterial ability by facile rechlorination, highlighting a valuable concept of renewable antibacterial coating in dental implant. These findings indicate an appealing application prospect for prevention and treatment of peri-implant infection.

Study of the role of Chlamydia, Mycoplasma, Ureaplasma and other microaerophilic and aerobic bacteria in uterine infections of mares with reproductive disorders

2003 ◽  
Vol 51 (1) ◽  
pp. 45-52 ◽  
Author(s):  
László Szeredi ◽  
M. Tenk ◽  
I. Schiller ◽  
Keyword(s):  
Pathogenic Bacteria ◽  
Anaerobic Bacteria ◽  
Aerobic Bacteria ◽  
Relative Importance ◽  
Reproductive Disorders ◽  
Taylorella Equigenitalis

In six healthy mares and 24 mares showing reproductive disorders swab samples were taken from the fossa clitoridis to isolate Taylorella equigenitalis, and from the uterus to isolate mycoplasmas, ureaplasmas and other aerobic bacteria. Swab samples were also taken from the uterus for Chlamydiaantigen ELISA and ChlamydiaPCR studies. The uterus of 27 mares was examined cytologically, and biopsy samples were taken from the endometrium for histological examinations and for immunohistochemical examinations aimed at the detection of chlamydiae. T. equigenitalis, mycoplasmas, ureaplasmas and chlamydiae could not be detected from any of the mares examined. Aerobic facultative pathogenic bacteria were isolated from mares with endometritis in four cases. In 18 out of 22 mares with endometritis (82%) no infective agents could be demonstrated. Further studies are needed to elucidate the relative importance of non-infectious causes of endometritis and of anaerobic bacteria often detectable in the uterus in the aetiology of the reproductive disorders observed.

Interaction of aerobic soil bacteria with plutonium(VI)

2001 ◽  
Vol 89 (8) ◽  
Author(s):  
P. Panak ◽  
Heino Nitsche
Keyword(s):  
Soil Bacteria ◽  
Aerobic Soil

We studied the interaction of Pu(VI) with

scholarly journals Liver Abscess Caused by Fusobacterium nucleatum: A Case Report

2021 ◽  
Vol 14 (4) ◽  
Author(s):  
Wenhao Wu ◽  
Wenjia Fan ◽  
Zhewen Zhou ◽  
Shouhao Wang ◽  
Chengan Xu ◽  
...  
Keyword(s):  
Liver Abscess ◽  
Pathogenic Bacteria ◽  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Pyogenic Liver Abscess ◽  
Microbial Detection ◽  
Specimen Collection ◽  
First Case ◽  
Positive Rate ◽  
Bacteria Culture

Introduction: Pyogenic liver abscess (PLA) is a serious infectious disease of the liver. PLA caused by Fusobacterium nucleatum is extremely rare. Here we report the first case of liver abscess caused by F. nucleatum in China. Case Presentation: The case was a 34-year-old female patient admitted to the hospital due to high fever. The diagnosis of liver abscess was confirmed by imaging studies and liver puncture. We finally confirmed the pathogen as F. nucleatum by next-generation sequencing (NGS). After the targeted anti-infective treatment, the patient recovered and discharged. Conclusions: As a new microbial detection method, NGS can still help in clinical practice. In addition, to improve the positive rate of anaerobic bacteria culture, we should pay attention to avoid contact with air in the process of specimen collection when the pathogenic bacteria are suspected to be anaerobic bacteria.

scholarly journals The neutrophil-mobilizing cytokine interleukin-26 in the airways of long-term tobacco smokers

2018 ◽  
Vol 132 (9) ◽  
pp. 959-983 ◽  
Author(s):  
Karlhans Fru Che ◽  
Ellen Tufvesson ◽  
Sara Tengvall ◽  
Elisa Lappi-Blanco ◽  
Riitta Kaarteenaho ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Bronchitis ◽  
Pathogenic Bacteria ◽  
Cell Model ◽  
Water Soluble ◽  
Chronic Obstructive ◽  
Neutrophil Accumulation

Long-term tobacco smokers with chronic obstructive pulmonary disease (COPD) or chronic bronchitis display an excessive accumulation of neutrophils in the airways; an inflammation that responds poorly to established therapy. Thus, there is a need to identify new molecular targets for the development of effective therapy. Here, we hypothesized that the neutrophil-mobilizing cytokine interleukin (IL)-26 (IL-26) is involved in airway inflammation amongst long-term tobacco smokers with or without COPD, chronic bronchitis or colonization by pathogenic bacteria. By analyzing bronchoalveolar lavage (BAL), bronchail wash (BW) and induced sputum (IS) samples, we found increased extracellular IL-26 protein in the airways of long-term smokers in vivo without further increase amongst those with clinically stable COPD. In human alveolar macrophages (AM) in vitro, the exposure to water-soluble tobacco smoke components (WTC) enhanced IL-26 gene and protein. In this cell model, the same exposure increased gene expression of the IL-26 receptor complex (IL10R2 and IL20R1) and nuclear factor κ B (NF-κB); a proven regulator of IL-26 production. In the same cell model, recombinant human IL-26 in vitro caused a concentration-dependent increase in the gene expression of NF-κB and several pro-inflammatory cytokines. In the long-term smokers, we also observed that extracellular IL-26 protein in BAL samples correlates with measures of lung function, tobacco load, and several markers of neutrophil accumulation. Extracellular IL-26 was further increased in long-term smokers with exacerbations of COPD (IS samples), with chronic bronchitis (BAL samples ) or with colonization by pathogenic bacteria (IS and BW samples). Thus, IL-26 in the airways emerges as a promising target for improving the understanding of the pathogenic mechanisms behind several pulmonary morbidities in long-term tobacco smokers.

scholarly journals A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice by in situ bioluminescence imaging

RSC Advances ◽  
2020 ◽  
Vol 10 (22) ◽  
pp. 13029-13036 ◽  
Author(s):  
Ning Zhao ◽  
Jing-Min Liu ◽  
Shuang Liu ◽  
Xue-Meng Ji ◽  
Huan Lv ◽  
...  
Keyword(s):  
Experimental Design ◽  
Pathogenic Bacteria ◽  
Bioluminescence Imaging ◽  
Reporter System ◽  
Bioluminescent Bacteria ◽  
Foodborne Bacteria ◽  
Food Borne

Schematic illustration of the preparation of bioluminescent bacteria and the experimental design of tracing of the foodborne bacteria in vivo.

Relationships between bacteria and cyanobacteria in the Marrakech waste stabilisation ponds

2000 ◽  
Vol 42 (10-11) ◽  
pp. 171-178 ◽  
Author(s):  
K. Oufdou ◽  
N. Mezrioui ◽  
B. Oudra ◽  
M. Barakate ◽  
M. Loudiki ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Mediterranean Climate ◽  
Treatment Process ◽  
Heterotrophic Bacteria ◽  
Sewage Treatment ◽  
Ecological Effect ◽  
E Coli ◽  
Waste Stabilisation Ponds ◽  
Cyanobacteria Blooms ◽  
Synechocystis Sp

In waste stabilisation pond systems the interactions between algae and bacteria have an important ecological effect and appeared to play a key role in the self-purification process. The aim of the present study is to evaluate the interactions between two axenic cyanobacteria: Synechocystis sp. and Pseudanabaena sp. with heterotrophic bacteria and some pathogenic bacteria: E. coli, Salmonella sp. and non-O1 V. cholerae. The results obtained showed that Synechocystis sp. (planktonic cyanobacterium) and Pseudanabaena sp. (benthic cyanobacterium) stimulated the growth and the survival of heterotrophic bacteria and non-O1 V. cholerae and reduced the survival of E. coli and Salmonella. Blooms of these cyanobacteria during hot periods which are a particularity in stabilisation ponds of Marrakech, could explain the dynamics of bacteria studied in this sewage treatment process. The presence of heterotrophic bacteria, showing relative high densities during hot periods, could be considered as an important bioticfactor which led to the cyanobacteria blooms in the Marrakech ponds which function under an arid Mediterranean climate.

scholarly journals Environmental Surveillance for Poliovirus and Other Enteroviruses: Long-Term Experience in Moscow, Russian Federation, 2004–2017

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 424 ◽  
Author(s):  
Olga E. Ivanova ◽  
Maria S. Yarmolskaya ◽  
Tatiana P. Eremeeva ◽  
Galina M. Babkina ◽  
Olga Y. Baykova ◽  
...  
Keyword(s):  
Sewage Treatment ◽  
Time Frame ◽  
Environmental Surveillance ◽  
Detection Rates ◽  
Echovirus 30 ◽  
Relative Value ◽  
Afp Surveillance ◽  
The One

Polio and enterovirus surveillance may include a number of approaches, including incidence-based observation, a sentinel physician system, environmental monitoring and acute flaccid paralysis (AFP) surveillance. The relative value of these methods is widely debated. Here we summarized the results of 14 years of environmental surveillance at four sewage treatment plants of various capacities in Moscow, Russia. A total of 5450 samples were screened, yielding 1089 (20.0%) positive samples. There were 1168 viruses isolated including types 1–3 polioviruses (43%) and 29 different types of non-polio enteroviruses (51%). Despite using the same methodology, a significant variation in detection rates was observed between the treatment plants and within the same facility over time. The number of poliovirus isolates obtained from sewage was roughly 60 times higher than from AFP surveillance over the same time frame. All except one poliovirus isolate were Sabin-like polioviruses. The one isolate was vaccine-derived poliovirus type 2 with 17.6% difference from the corresponding Sabin strain, suggesting long-term circulation outside the scope of the surveillance. For some non-polio enterovirus types (e.g., Echovirus 6) there was a good correlation between detection in sewage and incidence of clinical cases in a given year, while other types (e.g., Echovirus 30) could cause large outbreaks and be almost absent in sewage samples. Therefore, sewage monitoring can be an important part of enterovirus surveillance, but cannot substitute other approaches.

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