Acid Black 48 dye biosorption using Saccharomyces cerevisiae immobilized with treated sugarcane bagasse

2012 ◽  
Vol 66 (7) ◽  
pp. 1431-1438 ◽  
Author(s):  
E. K. Mitter ◽  
C. R. Corso

The textile industry consumes large quantities of water and chemicals, especially in dyeing and finishing processes. Textile dye adsorption can be accomplished with natural or synthetic compounds. Cell immobilization using biomaterials allows the reduction of toxicity and mechanical resistance and opens spaces within the matrix for cell growth. The use of natural materials, such as sugarcane bagasse, is promising due to the low costs involved. The aim of the present study was to evaluate the use of sugarcane bagasse treated with either polyethyleneimine (PEI), NaOH or distilled water in the cell immobilization of Saccharomyces cerevisiae for textile dye removal. Three different adsorption tests were conducted: treated sugarcane bagasse alone, free yeast cells and bagasse-immobilized yeast cells. Yeast immobilization was 31.34% with PEI-treated bagasse, 8.56% with distilled water and 22.54% with NaOH. PEI-treated bagasse exhibited the best removal rates of the dye at all pH values studied (2.50, 4.50 and 6.50). The best Acid Black 48 adsorption rates were obtained with use of free yeast cells. At pH 2.50, 1 mg of free yeast cells was able to remove 5488.49 g of the dye. The lowest adsorption capacity rates were obtained using treated bagasse alone. However, the use of bagasse-immobilized cells increased adsorption efficiency from 20 to 40%. The use of immobilized cells in textile dye removal is very attractive due to adsorbed dye precipitation, which eliminates the industrial need for centrifugation processes. Dye adsorption using only yeast cells or sugarcane bagasse requires separation methods.

2008 ◽  
Vol 14 (4) ◽  
pp. 235-238 ◽  
Author(s):  
Vesna Vucurovic ◽  
Radojka Razmovski ◽  
Mario Rebic

This study provides a preliminary contribution to the development of a bioprocess for the production of ethanol using Saccharomyces cerevisiae cells immobilized onto a corn stem. For this purpose, the yeast cells were submitted to the batch tests in situ adsorption onto 0.5 cm long corn stem. Cells immobilization was analyzed by optical microscopy. The number of the yeast cells, fermentation kinetics, the ethanol yield in the presence or the absence of the support in the fermentation medium was investigated. It was determined that the addition of the corn stem led to the abrupt increase of the yeast cells number in substrate, ethanol yield, pH value, a total dissolved salts content and substrate conductivity. The addition of 5 and 10g of the corn stem pith per liter of the medium decreased the amount of residual sugar. The results indicate that a corn stem might be a good carrier for the yeast cell immobilization, and also a cheap alternative recourse of mineral components with the possibility of application for improving ethanol productivities.


REAKTOR ◽  
2018 ◽  
Vol 18 (03) ◽  
pp. 127 ◽  
Author(s):  
Sita Heris Anita ◽  
Wibowo Mangunwardoyo ◽  
Yopi Yopi

Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse


2019 ◽  
Vol 7 (10) ◽  
pp. 404 ◽  
Author(s):  
Ana Paula Pereira ◽  
Ana Mendes-Ferreira ◽  
Luís G. Dias ◽  
José M. Oliveira ◽  
Leticia M. Estevinho ◽  
...  

Mead is a traditional beverage that results from the alcoholic fermentation of diluted honey performed by yeasts. Although the process of mead production has been optimized in recent years, studies focused on its sensory properties are still scarce. Therefore, the aim of this work was to analyse the sensory attributes of mead produced with free or immobilized cells of the Saccharomyces cerevisiae strains QA23 and ICV D47, and to establish potential correlations with its volatile composition. In the volatile composition of mead, the effect of yeast condition was more important than the strain. In respect to sensory analysis, the most pleasant aroma descriptors were correlated with mead obtained with free yeast cells, independently of the strain. Both sensory analysis and volatile composition indicates that the most pleasant mead was produced by free yeast cells. Although this study has provided a significant contribution, further research on the sensory quality of mead is still needed.


2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Gamal Owes El-Sayed ◽  
Talaat Younis Mohammed ◽  
Ashraf Abd-Allah Salama

Sugarcane stalks powder was tested for its efficiency of removing a textile dye Maxilon Red GRL from aqueous solution. Different parameters affecting dye removal efficiency were studied. These parameters include contact time, initial dye concentration, adsorbent dose, ionic strength, pH, and temperature. Langmuir and Freundlich isotherm models were applied to the equilibrium data. The data fitted well with the Langmuir isotherm (). The maximum monolayer adsorption capacity () was found to be 20.96 mg/g at an initial pH of 7.2. The temperature variation study showed that dye adsorption is exothermic and spontaneous with increased randomness at the solid solution interface. The results indicated that sugarcane stalks could be an alternative for more costly adsorbents used for dye removal. The kinetic of the adsorption process followed the pseudo second-order kinetics model.


2013 ◽  
Vol 20 (3) ◽  
pp. 73
Author(s):  
Rosiane Ferreira do Nascimento ◽  
Cícero Wellington Brito Bezerra ◽  
Hildo Antonio dos Santos Silva ◽  
Mário Sérgio Schultz ◽  
Sirlane Aparecida Abreu Santana

Abstract: In this work water hyacinth (Eichhornia crassipes) was used as adsorbent for aqueous Violet Remazol 5R (VR 5) solutions. In order to obtain the best conditions for the removal, the pH influence, time of contact and concentration were verified. The adsorption showed itself highly dependent of the pH, being the maximum adsorption was observed pH 1.0. In the kinetic experiments, the batchwise method was used with concentrations of 250 and 1000 mg dm-³, and after the time of contact the concentration was determined by spectrophotometry. An adequation of the models of pseudo-first and second order was made based on the results that were found. The second order model was the most suitable in both concentrations,with R²= 0.999. The Langmuir, Freundlich, Sips and Temkin models were used to analyze the experimental data in its linearized mode. The Langmuir model was the most appropriate. In the conditions studied, the removal of textile dye Violet Remazol 5R by water hyacinth showed good results, making it possible to use this material as an alternative way to remove dyes from textile effluents.Keywords: Water hyacinth (Eichhornia crassipes). Textile dye. Adsorption. EFEITO DO pH E MELHORIA NA REMOÇÃO DO CORANTE VIOLETA REMAZOL EM ÁGUA POR Eichhornia Crassipes (aguapé)Resumo: Neste trabalho foi utilizado o aguapé (Eichhornia crassipes) como adsorvente de soluções aquosasde Violeta Remazol 5R (VR 5). De modo a obter as melhores condições para a remoção foram verificados,a influência do pH, tempo de contato e a concentração. A adsorção mostrou-se altamente dependente do pH, sendo que a adsorção máxima foi observada pH 1,0. Nas experiências de cinética, o método de batelada foi utilizado nas concentrações de 250 e 1000 mg dm-3, e após o tempo de contato com a concentraçãofoi determinada por espectrofotometria. Uma adequação dos modelos de pseudo-primeira ordem e segunda foi feita com base nos resultados que foram encontrados. O modelo de segunda ordem foi o mais adequado em ambas às concentrações, com R ² = 0,999. Os modelos de Langmuir, Freundlich, Sips e Temkin foram utilizados para analisar os dados experimentais em seu modo linear. O modelo de Langmuir foi o mais adequado. Nas condições estudadas, a remoção de corante têxtil Violeta Remazol 5R por aguapé mostrou bons resultados, tornando possível a utilização deste material como uma forma alternativa para remover corantes de efluentes têxteis.Palavras-chave: Aguapé (Eichhornia crassipes). Corante têxtil. Adsorção. EFECTO DEL pH Y LA MEJORA EN REMOVAL TINTE DE REMAZOL VIOLET DEL AGUA POR Eichhornia Crassipes (jacinto de agua)Resumen: En este trabajo fue utilizado el jacinto de agua (Eichhornia crassipes) como adsorvente para la remoción del colorante textil aniónico Violeta de Remazol 5R de soluciones acuosas. Con el objetivo de encontrar condiciones mas adecuadas para la remoción, se verificó la influencia del pH, tiempo de contactoy concentración. La adsorción se mostró fuertemente dependiente del pH, presentando una adsorción máxima a pH 1,0. Para los experimentos de cinética se utilizou el método de lote en las concentraciones de 250 y 1000 mg dm-3, y después del tiempo de contacto se realizó la determinación de la concentración atraves de espectrofotometria. Con los resultados conseguidos se hizo una adaptación a los modelos de pseudo-primera orden y segunda orden, con R2 = 0,999. Los modelos de Langmuir, Freundlich e Temkin, fueron utilizados para analizar los datos experimentales en su forma linealizada. El modelo que major se adaptó fue el de Langmuir. Bajo las condiciones de estudio, la remoción del colorante textil aniónico Violeta de Remazol 5R por jacinto de agua mostró buenos resultados, lo que permitiria que pueda ser utilizado como una alternativa para la remoción de colorantes en efluentes textiles.Palabras clave: Jacinto de agua (Eichhornia crassipes). Colorante textil. Adsorción.


Author(s):  
E. Keyhani

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).


Author(s):  
Abdallah Reghioua ◽  
Djamel Barkat ◽  
Ali H. Jawad ◽  
Ahmed Saud Abdulhameed ◽  
Abdullah A. Al-Kahtani ◽  
...  

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 724
Author(s):  
Miguel L. Sousa-Dias ◽  
Vanessa Branco Paula ◽  
Luís G. Dias ◽  
Letícia M. Estevinho

This work studied the production of mead using second category honey and the immobilized cells of Saccharomyces cerevisiae in sodium alginate, with concentrations of 2% and 4%, and their reuse in five successive fermentations. The immobilized cells with 4% alginate beads were mechanically more stable and able to allow a greater number of reuses, making the process more economical. The fermentation’s consumption of sugars with free cells (control) and immobilized cells showed a similar profile, being completed close to 72 h, while the first use of immobilized cells finished at 96 h. The immobilized cells did not significantly influence some oenological parameters, such as the yield of the consumed sugars/ethanol, the alcohol content, the pH and the total acidity. There was a slight increase in the volatile acidity and a decrease in the production of SO2. The alginate concentrations did not significantly influence either the parameters used to monitor the fermentation process or the characteristics of the mead. Mead fermentations with immobilized cells showed the release of cells into the wort due to the disintegration of the beads, indicating that the matrix used for the yeast’s immobilization should be optimized, considering the mead production medium.


Author(s):  
Florencia Ruscasso ◽  
Brenda Bezus ◽  
Gabriela Garmendia ◽  
Silvana Vero ◽  
Gustavo Curutchet ◽  
...  

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