Composición química del aceite esencial de Aloysia Triphylla "cedrón" como insumo para la elaboración de un enjuague bucal

2021 ◽  
Vol 8 (2) ◽  
pp. 64-67
Author(s):  
Jenny Rosalyn Huerta León ◽  
Jhonnel Williams Samaniego Joaquin ◽  
Cesar Máximo Fuertes Ruitón
Keyword(s):  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Aloysia Triphylla

Objetivo: Determinar la actividad antimicrobiana del aceite esencial obtenidos de las hojas y tallos de Aloysia triphylla “cedrón” provenientes de la ciudad de Huaraz, departamento de Ancash, distrito de Mancos. Materiales y métodos: Se utilizó el método de hidrodestilación por arrastre de vapor para la obtención del aceite esencial y para la determinación de la actividad antibacteriana se realizó por la metodología de difusión de agar frente a Streptococcus mutans ATCC 25175 y Porphyromonas gingivalis ATCC 33277 comparándolas con un control positivo el cual era un enjuague bucal comercial de clorhexidina al 0,05%. Resultados: Se obtuvo un rendimiento del 0,11 y en el estudio microbiológico se obtuvieron halos de 21,3 mm del aceite esencial y 19,9 mm del control positivo frente a Streptococcus mutans ATCC 25175 y de 35,5 mm en el aceite esencial y 25,4 mm del control positivo frente a Porphyromonas gingivalis ATCC 33277. Conclusiones: Se concluye que, que la actividad antimicrobiana que demostró el aceite esencial de Aloysia triphylla (cedrón) es superior al control positivo que fue un enjuague bucal comercial de clorhexidina al 0,05%, pudiendo este ser usado como fuente en la elaboración de preparaciones farmacéuticas en el tratamiento de infecciones causadas por estos microorganismos.

Antibacterial Activity of Silver-Hydroxyapatite/Titania Nanocomposite Coating on Titanium against Oral Bacteria

2007 ◽  
Vol 330-332 ◽  
pp. 455-458 ◽  
Author(s):  
An Chun Mo ◽  
Wei Xu ◽  
Su Qin Xian ◽  
Yu Bao Li ◽  
Shi Bai
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Nanocomposite Coating ◽  
Oral Bacteria ◽  
Tio2 Coating ◽  
Prevotella Intermedia ◽  
Anaerobic Incubation ◽  
Coating Method ◽  
Coated Surfaces

This study was focused on evaluating the bactericidal and anti-adhesive efficacy of silver-hydroxyapatite/ titania nanocomposites (nAg-HA/TiO2) coating on titanium against oral bacteria. Porphyromonas gingivalis, Prevotella intermedia and Fusohacterium nucleatum and Streptococcus mutans were used. Antibacterial activity of nAg-HA/TiO2 coating was investigated quantitatively using film applicator coating method and titanium plates incubated with bacteria were prepared for SEM to observe the adherence of oral bacteria. The viability of each type of bacteria on the antibacterial film was suppressed to about 10% after anaerobic incubation for 3 hours. Image of SEM demonstrated that bacteria on sandblasting surfaces were relatively confluent whilst on coated surfaces fewer bacteria were observed. Adherence of bacteria on nAg-HA / TiO2-coated surfaces compared with uncoated surfaces was remarkably decreased.

scholarly journals Antibacterial efficacy of commercially available activated carbon tooth powder: An in vitro study

2020 ◽  
Vol 11 (4) ◽  
pp. 6748-6753
Author(s):  
Padmaja Vangipuram ◽  
Ravishankar P L ◽  
Prem Blaisie Rajula M ◽  
Rajarajeswari S ◽  
Saravanan A V ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Antimicrobial Agents ◽  
Brain Heart Infusion ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Carbon Powder ◽  
Cow Dung

There has been an indiscriminate use of commercial antimicrobials in the previous decades, leading to emergence of multidrug resistant bacteria. This has become a frequent occurrence, so natural antimicrobial agents have grabbed attention of researchers as potential alternatives. Of particular interest is cow dung which has been shown to have antibacterial and antiseptic properties. It is known to be used predominantly in rural areas. This paper aims to evaluate the antibacterial activity of Goshala Activated Carbon toothpowder against three strains of periodontopathogennic and cariogenic bacteria. Standardized strains of Streptococcus mutans, Aggregatibacter actinomycetemcomitans  and Porphyromonas gingivalis were cultured in BHI (Brain Heart Infusion Broth) media and Minimum Inhibitory Concentration (MIC) of Activated Carbon tooth powder was assessed by serial dilution method. Likewise, Amoxicillin, Metronidazole and Chlorhexidine were tested against the same pathogens. Porphyromonas gingivalis, Streptococcus mutans  and Aggregatibacter actinomycetemcomitans were shown to be sensitive at an MIC of 50mg/ml, 25mg/ml and 0.8mg/ml for activated carbon toothpowder respectively. Given some limitations of this study, we can conclude that  activated carbon powder presents a ray of hope in developing a targeted agent for aggressive periodontitis patients.

scholarly journals EFFECTIVENESS OF A JAVANESE TURMERIC ETHANOL EXTRACT FOR ERADICATING STREPTOCOCCUS MUTANS AND PORPHYROMONAS GINGIVALIS BIOFILMS

2019 ◽  
pp. 27-31
Author(s):  
DEWI FATMA SUNIARTI ◽  
AGOENG TJAHAJANI SARWONO ◽  
MARINA ROSYANA
Keyword(s):  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Growth Phase ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Growth Phases

Objective: We aimed to determine the effectiveness of an identified Javanese turmeric ethanolic extract (IIJTEE) for eradicating biofilms formed byStreptococcus mutans, Porphyromonas gingivalis, or both.Methods: Such biofilms during different growth phases were exposed to various concentrations of an IJTEE to determine its effects on bacterialproliferation.Results: The effectiveness of the IJTEE in eradicating the S. mutans biofilm was concentration-dependent but not when used to treat P. gingivalis andS. mutans - P. gingivalis biofilms.Conclusion: The effectiveness of the IJTEE for eradicating biofilms formed by S. mutans, P. gingivalis, and S. mutans plus P. gingivalis biofilms dependedon the growth phase of the biofilm. Thus, IJTEE eradicated biofilms formed by S. mutans, P. gingivalis, or both.

scholarly journals Bioactive Compounds Isolated from Indonesian Epiphytic Plant of Sarang Semut and Their Antibacterial Activity against Pathogenic Oral Bacteria

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Dikdik Kurnia ◽  
Dadan Sumiarsa ◽  
Hendra D.A. Dharsono ◽  
Mieke H. Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Steroid Glycoside ◽  
Oral Bacteria ◽  
Epiphytic Plant

A new phenolic compound (1), a steroid (2), a new steroid glycoside (4), two triterpenoids (3 & 6) and a new phloroglucinol-sesquiterpene (5) have been isolated from the ethyl acetate extract of Sarang Semut ( Myrmecodia pendans) and their structures were determined on the basis of the spectral data. The bioactivity evaluation was conducted with the inhibition zone of compounds (mm) using Kirby-Bauer method at concentrations of 1000 and 5000 ppm for compound 1 against pathogenic oral bacteria Enterococcus faecalis, was 8.55 and 8.05 mm, respectively. Compounds 2–3 against Streptococcus mutans were 9.00 and 8.45 mm (2) and 10.24 and 9.35 mm (3), respectively. Compound 5 against Porphyromonas gingivalis was 11.5 and 10.8 mm, respectively.

scholarly journals In Vitro Evaluation of Bacterial Adhesion and Bacterial Viability of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis on the Abutment Surface of Titanium and Zirconium Dental Implants

2019 ◽  
Vol 2019 ◽  
pp. 1-5 ◽  
Author(s):  
Ana Stefany Meza-Siccha ◽  
Miguel Angel Aguilar-Luis ◽  
Wilmer Silva-Caso ◽  
Fernando Mazulis ◽  
Carolina Barragan-Salazar ◽  
...  
Keyword(s):  
Dental Implants ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Bacterial Adhesion ◽  
Bacterial Species ◽  
Bacterial Adherence ◽  
Bacterial Viability ◽  
Streptococcus Sanguinis ◽  
Group 2

Objective. To evaluate the in vitro adherence and viability of 3 bacterial species Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277) on the surfaces of dental implants of titanium, zirconium, and their respective fixing screws. Methods. Two analysis groups were formed: group 1 with 3 titanium pillars and group 2 with 3 zirconium pillars, each with their respective fixing screws. Each of these groups was included in tubes with bacterial cultures of Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277). These samples were incubated at 37°C under anaerobic conditions. Bacterial adherence was assessed by measurement of the change in colony-forming units (CFU), and bacterial viability was evaluated with the colorimetric test of 3-(4,5-dimethylthiazol-2)-2,5 diphenyl tetrazolium bromide (MTT). Results. The bacterial adhesion in the titanium abutments was higher for Streptococcus mutans (190.90 CFU/mL), and the viability was greater in Porphyromonas gingivalis (73.22%). The zirconium abutment group showed the highest adherence with Streptococcus mutans (331.82 CFU/mL) and the highest bacterial viability with the S. sanguinis strain (38.42%). The titanium fixation screws showed the highest adhesion with S. sanguinis (132.5 CFU/mL) compared to the zirconium fixation screws where S. mutans had the highest adhesion (145.5 CFU/mL). The bacterial viability of S. mutans was greater both in the titanium fixation screws and in the zirconium fixation screws 78.04% and 57.38%, respectively. Conclusions. Our results indicate that there is in vitro bacterial adherence and viability in both titanium abutments and zirconium abutments and fixation screws for both. Streptococcus mutans is the microorganism that shows the greatest adherence to the surfaces of both titanium and zirconium and the fixing screws of the latter. On the contrary, bacterial viability is greater on the titanium abutments with P. gingivalis than on the zirconium abutments with S. sanguinis. With respect to the fixation screws, in both cases, the viability of S. mutans was greater with respect to the other bacteria. In general, the titanium abutments showed less adherence but greater bacterial viability.

scholarly journals Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities

2019 ◽  
Vol 87 (1) ◽  
pp. 2 ◽  
Author(s):  
Luis Sequeda-Castañeda ◽  
Camila Muñoz-Realpe ◽  
Crispín Celis-Zambrano ◽  
Sandra Gutiérrez-Prieto ◽  
Pilar Luengas-Caicedo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Fusobacterium Nucleatum ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Prevotella Intermedia ◽  
Streptococcus Sobrinus ◽  
Total Extract ◽  
Liquid Fractionation

Berberis goudotii is an endemic Colombian plant found in the paramo ecosystem. It has been used in food preparation and as a medicinal plant for diverse treatments. Additionally, it is used as a mouthwash to strengthen the gums and combat throat irritations and periodontitis. The present research evaluated Berberis goudotii aerial parts extract and fractions antimicrobial activities. Ultrasonic-assisted extraction was used to attain total ethanol-water extract. Solid-liquid fractionation was used to obtain hexane fraction. The residue was dispersed in water and liquid-liquid fractionation was carried-out to acquire dichloromethane, butanol and water fractions. Preliminary phytochemical analysis was performed on total extract and phenol, polyphenol, flavonoid, and proanthocyanidin, while tannin content was quantified. Antimicrobial activity assessment was performed by agar diffusion method using disks and wells employing Ceftazidime as a positive control against Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus casei, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum. Antimicrobial activity was determined as relative percentage inhibition (RPI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phenols (92.5 ± 7.7 mg GA/10 g), polyphenols (87.7 ± 8.1 mg PG/10 g) and tannins (44.1 ± 4.3 mg PG/10 g) were among the highest secondary metabolites observed. Total extract presented an MBC of 1.0 µg/µL against cariogenic bacteria (Streptococcus mutans and Streptococcus sobrinus) and 0.12 µg/µL against bacteria associated with periodontal disease (Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum). Butanol and hexane fractions showed antiperiodontal activity with MBC of 0.12 and 1.0 µg/µL, respectively. In conclusion, Berberis goudotii total extract demonstrated antimicrobial activity against cariogenic and periodontal microorganisms, on the other hand, hexane and butanol fractions displayed antiperiodontal activity.

Isolation of the new anacardic acid 6-[16′Z-nonadecenyl]-salicylic acid and evaluation of its antimicrobial activity against Streptococcus mutans and Porphyromonas gingivalis

2011 ◽  
Vol 25 (13) ◽  
pp. 1282-1287 ◽  
Author(s):  
Blanca E. Rivero-Cruz ◽  
Nuria Esturau ◽  
Sobeida Sánchez-Nieto ◽  
Irma Romero ◽  
Israel Castillo-Juárez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Salicylic Acid ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Anacardic Acid
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