High Level Gap Analysis for Accident Tolerant and Advanced Fuels for Storage and Transportation.

African lions (Panthera leo) are commercially farmed across South Africa for sport hunting, tourism, and the international bone trade, primarily in Southeast Asia. Despite its legal status, South Africa’s growing lion farming industry is a contentious issue. In 2020 a high-level panel was initiated to review the policies, legislation, and management regarding the breeding, hunting, trade, and handling of four wildlife species, including lions. In May 2021, it was announced that the government intends to amend existing permit conditions to prohibit lion breeding and tourism interactions with captive lions, as well as to stop issuing permits to new entrants into the industry, effectively ending lion farming. In order to follow this line of action, a comprehensive, well-managed plan will be necessary to execute a responsible exit from the industry as it currently stands. Using a “gap analysis” management tool, we aim to: (1) outline some of the key considerations regarding the current state of the lion farming industry in South Africa; and (2) propose specific action steps that could be taken within five key areas (regulation, animal welfare, health and safety, equitability, and conservation) to help inform a responsible transition away from this type of wildlife farming in the biodiversity economy. For our gap analysis, we conducted a semi-systematic literature search to compile key background information about the current state of the industry. This information was then used to identify corresponding desired management states, and steps that could facilitate a successful phase out of lion farming in South Africa. We hope our approach helps identify key considerations for a responsible transition and can help aid decisions during the management of this process.

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Evaluation of Ground Handling, Pax on Board and Hotel Crew Business Processes in Aviation Company

Advanced Science Letters ◽

10.1166/asl.2015.5899 ◽

2015 ◽

Vol 21 (4) ◽

pp. 618-621

Author(s):

Deni Mariana ◽

Stefie Cuhadi ◽

Sanny Khosasi ◽

. Noerlina

Keyword(s):

Business Process ◽

Qualitative Data ◽

Business Processes ◽

Gap Analysis ◽

Research Data ◽

Materials Management ◽

Application System ◽

Evaluation Result ◽

Ground Handling ◽

High Level

This research aims to explain more about problems that was in G.P.H (Ground Handling, Pax on Board and Hotel Crew) application system of Materials Management in PT Garuda Indonesia. Problems that was found in the respective company will be analysed through Fit/Gap Analysis as well as Risk Analysis. Research data will be processed through qualitative data which came from internship programme, interview, observation and evaluation. Result from Fit/Gap Analysis showed that majority of risk that occured could be categorized in High High level. Thus, it could be concluded that problems which had been found could be resolved by having reengineering the business process, provide training to the users involved and conduct periodic evaluations.

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Adoption Gap Analysis in Tomato Cultivation in Banda District of Bundelkhand (U.P.)

10.48165/ijee.2021.57427 ◽

2021 ◽

Vol 57 (4) ◽

pp. 126-130

Author(s):

B. K. Gupta ◽

S. V. Dwivedi ◽

B. P. Mishra ◽

Dheeraj Mishra ◽

Pankaj K. Ojha ◽

...

Keyword(s):

Seed Treatment ◽

Gap Analysis ◽

Plant Protection ◽

Uttar Pradesh ◽

Fertilizer Application ◽

Vegetable Crops ◽

Protection Measures ◽

Technological Gap ◽

Block Based ◽

High Level

Bundelkhand area of Uttar Pradesh is comprised of seven districts viz., Chitrakoot, Banda, Hamirpur, Mahoba, Jalaun, Jhansi and Lalitpur. The present study was conducted in Banda district of Uttar Pradesh where the vegetable crops are grown by farmers in large areas. Among the eight blocks of Banda, two blocks namely Badokhar Khurd and Mahua were purposively selected. Eight vegetable growing villages (4 from Badokhar Khurd and 4 from Mahua block) based on production potential of the tomato were drawn up and from each village 18 vegetable growers (six from each category) having minimum 3 years of experience in commercial tomato cultivation were selected randomly with sample size is 144. It is revealed that technological gap in marginal farmers category was observed in the nursery raising and seed-treatment (74.80%) followed by plant protection measures (67.83%) and fertilizer application (67.00%). In small farmers category Identification of insect and pest (77.16%) followed by fertilizer application (74.50%) and plant protection measures (67.33%) were major whereas for medium category the technological gap was observed in nursery raising and seed-treatment (69.30%) followed by plant protection measures (67.58%) and fertilizer application (66.17%). The technological gap level in all categories of farmers revealed that the medium level of technological gap was observed in majority of tomato growers (44.45%) followed by high level of technological gap (38.20%) and low level of technological gap (17.36%).

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Strengthening multi-hazards early warning system in the pacific through BMKG-UNESCAP collaboration pilot projects

MATEC Web of Conferences ◽

10.1051/matecconf/201822904019 ◽

2018 ◽

Vol 229 ◽

pp. 04019

Author(s):

Asteria S. Handayani ◽

Marjuki ◽

Agie Wandala Putra ◽

Urip Haryoko ◽

Nurhayati ◽

...

Keyword(s):

Early Warning ◽

Early Warning System ◽

Gap Analysis ◽

Solomon Islands ◽

Regional Scale ◽

Warning System ◽

Pilot Projects ◽

Implementation Phase ◽

The Pacific ◽

High Level

On the period of September to December 2017, three pilot projects were implemented in Tonga, Papua New Guinea, and Solomon Islands aiming to strengthen the multi-hazards early warning system in the respective countries through close collaboration between the Indonesian Agency for Meteorology Climatology and Geophysics (BMKG) and United Nations Economic and Social Commission for Asia and the Pacific (UNESCAP). Main activities during the implementation phase were tailored based on gap analysis and risk assessments conducted beforehand. Thus, installation of high-resolution numerical weather, ocean wave, and climate prediction and forecasting tools were chosen to fill in the assessed gaps. These activities were incorporated with capacity building activities and high-level meetings with related stakeholders in disaster risk management using the concept of Fast-Leveraging-Easy-Economical-Sustain (FLEES). All three pilot projects had successfully proven to achieve their objectives by improving the capacities of National Meteorological Services in those three countries to produce multi-hazards early warning in higher resolution at a regional scale for disaster management in their respective countries.

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Taking stock of decentralized disaster risk reduction in Indonesia

Natural Hazards and Earth System Science ◽

10.5194/nhess-16-2145-2016 ◽

2016 ◽

Vol 16 (9) ◽

pp. 2145-2157 ◽

Cited By ~ 4

Author(s):

Anthony Grady ◽

Berry Gersonius ◽

Alexandros Makarigakis

Keyword(s):

Local Government ◽

Risk Reduction ◽

Disaster Risk Reduction ◽

Gap Analysis ◽

Analytical Framework ◽

Disaster Risk ◽

Structured Interviews ◽

Barriers To Implementation ◽

Communication And Coordination ◽

High Level

Abstract. The Sendai Framework, which outlines the global course on disaster risk reduction until 2030, places strong importance on the role of local government in disaster risk reduction. An aim of decentralization is to increase the influence and authority of local government in decision making. Yet, there is limited empirical evidence of the extent, character and effects of decentralization in current disaster risk reduction implementation, and of the barriers that are most critical to this. This paper evaluates decentralization in relation to disaster risk reduction in Indonesia, chosen for its recent actions to decentralize governance of DRR coupled with a high level of disaster risk. An analytical framework was developed to evaluate the various dimensions of decentralized disaster risk reduction, which necessitated the use of a desk study, semi-structured interviews and a gap analysis. Key barriers to implementation in Indonesia included: capacity gaps at lower institutional levels, low compliance with legislation, disconnected policies, issues in communication and coordination and inadequate resourcing. However, any of these barriers are not unique to disaster risk reduction, and similar barriers have been observed for decentralization in other developing countries in other public sectors.

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Assessing accuracy and completeness of GenBank for eDNA metabarcoding: towards a reliable marine fish reference database

ARPHA Conference Abstracts ◽

10.3897/aca.4.e64671 ◽

2021 ◽

Vol 4 ◽

Author(s):

Cristina Claver ◽

Oriol Canals ◽

Naiara Rodriguez-Ezpeleta

Keyword(s):

Ribosomal Rna ◽

Gap Analysis ◽

Environmental Dna ◽

Reference Database ◽

Fish Diversity ◽

Cyt B ◽

Taxonomic Assignment ◽

Reference Databases ◽

Taxonomic Groups ◽

High Level

Environmental DNA (eDNA) metabarcoding, the process of sequencing DNA collected from the environment for producing biodiversity inventories, is increasingly being applied to assess fish diversity and distribution in marine environments. Yet, the successful application of this technique deeply relies on accurate and complete reference databases used for taxonomic assignment. The most used markers for fish eDNA metabarcoding studies are the cytochrome C oxidase subunit 1 (COI), 16S ribosomal RNA (16S), the 12S ribosomal RNA (12S) and cytochrome b (cyt b) genes, whose sequences are usually retrieved from GenBank, the largest DNA sequence database that represents a worldwide public resource for genetic studies. Thus, the completeness and accuracy of GenBank is critical to derive reliable estimations from fish eDNA metabarcoding data. Here, we have i) compiled the checklist of European marine fishes, ii) performed a gap analysis of the four genes and, within COI and 12S, also of the most used barcodes for fish, and iii) developed a workflow to detect potentially incorrect records in GenBank. We found that from the 1965 species in the checklist (1761 Actinopterygii, 189 Elasmobranchii, 9 Holocephali, 4 Petromyzonti and 2 Myxini), about 70% have sequences for COI, whereas less have sequences for 12S, 16S and cyt b (45-55%). Among the species for which COI ad 12S sequences are available, about 60% and 40% have sequences covering the most used barcodes respectively. The analysis of pairwise distances between sequences revealed pairs belonging to the same species with significantly low similarity and pairs belonging to different high level taxonomic groups (class, order) with significantly large similarity. In light of this further confirmation of presence of a substantial number of incorrect records in GenBank, we propose a method for identifying and removing spurious sequences to create reliable and accurate reference databases for eDNA metabarcoding.

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Transcription factor/DNA interactions visualized by electron spectroscopic imaging

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100122654 ◽

1992 ◽

Vol 50 (1) ◽

pp. 450-451

Author(s):

David P. Bazett-Jones ◽

Mark L. Brown

Keyword(s):

Transcription Factor ◽

Dna Sequences ◽

Transcription Initiation ◽

Molecular Mechanisms ◽

Phosphorus Content ◽

Spectroscopic Imaging ◽

Electron Spectroscopic Imaging ◽

Dna Backbone ◽

Two Parameters ◽

High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

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Advances in Stem Instrumentation for Biology

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100180562 ◽

1990 ◽

Vol 48 (1) ◽

pp. 362-363

Author(s):

J. S. Wall

Keyword(s):

Scanning Transmission Electron Microscope ◽

Carbon Films ◽

Specimen Preparation ◽

Material Deposition ◽

Active User ◽

Percent Improvement ◽

Scanning Transmission ◽

The Moment ◽

Film Substrate ◽

High Level

The forte of the Scanning transmission Electron Microscope (STEM) is high resolution imaging with high contrast on thin specimens, as demonstrated by visualization of single heavy atoms. of equal importance for biology is the efficient utilization of all available signals, permitting low dose imaging of unstained single molecules such as DNA.Our work at Brookhaven has concentrated on: 1) design and construction of instruments optimized for a narrow range of biological applications and 2) use of such instruments in a very active user/collaborator program. Therefore our program is highly interactive with a strong emphasis on producing results which are interpretable with a high level of confidence.The major challenge we face at the moment is specimen preparation. The resolution of the STEM is better than 2.5 A, but measurements of resolution vs. dose level off at a resolution of 20 A at a dose of 10 el/A2 on a well-behaved biological specimen such as TMV (tobacco mosaic virus). To track down this problem we are examining all aspects of specimen preparation: purification of biological material, deposition on the thin film substrate, washing, fast freezing and freeze drying. As we attempt to improve our equipment/technique, we use image analysis of TMV internal controls included in all STEM samples as a monitor sensitive enough to detect even a few percent improvement. For delicate specimens, carbon films can be very harsh-leading to disruption of the sample. Therefore we are developing conducting polymer films as alternative substrates, as described elsewhere in these Proceedings. For specimen preparation studies, we have identified (from our user/collaborator program ) a variety of “canary” specimens, each uniquely sensitive to one particular aspect of sample preparation, so we can attempt to separate the variables involved.

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A Questionnaire Survey of Current Rehabilitation Practices for Adults With Normal Hearing Sensitivity Who Experience Auditory Difficulties

American Journal of Audiology ◽

10.1044/2020_aja-20-00027 ◽

2020 ◽

Vol 29 (4) ◽

pp. 738-761

Author(s):

Tess K. Koerner ◽

Melissa A. Papesh ◽

Frederick J. Gallun

Keyword(s):

Hearing Aids ◽

Questionnaire Survey ◽

Patient Population ◽

Clinical Care ◽

Normal Hearing ◽

Auditory Training ◽

Training Methods ◽

Hearing Sensitivity ◽

Considerable Benefit ◽

High Level

Purpose A questionnaire survey was conducted to collect information from clinical audiologists about rehabilitation options for adult patients who report significant auditory difficulties despite having normal or near-normal hearing sensitivity. This work aimed to provide more information about what audiologists are currently doing in the clinic to manage auditory difficulties in this patient population and their views on the efficacy of recommended rehabilitation methods. Method A questionnaire survey containing multiple-choice and open-ended questions was developed and disseminated online. Invitations to participate were delivered via e-mail listservs and through business cards provided at annual audiology conferences. All responses were anonymous at the time of data collection. Results Responses were collected from 209 participants. The majority of participants reported seeing at least one normal-hearing patient per month who reported significant communication difficulties. However, few respondents indicated that their location had specific protocols for the treatment of these patients. Counseling was reported as the most frequent rehabilitation method, but results revealed that audiologists across various work settings are also successfully starting to fit patients with mild-gain hearing aids. Responses indicated that patient compliance with computer-based auditory training methods was regarded as low, with patients generally preferring device-based rehabilitation options. Conclusions Results from this questionnaire survey strongly suggest that audiologists frequently see normal-hearing patients who report auditory difficulties, but that few clinicians are equipped with established protocols for diagnosis and management. While many feel that mild-gain hearing aids provide considerable benefit for these patients, very little research has been conducted to date to support the use of hearing aids or other rehabilitation options for this unique patient population. This study reveals the critical need for additional research to establish evidence-based practice guidelines that will empower clinicians to provide a high level of clinical care and effective rehabilitation strategies to these patients.

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