Scanning Electron Microscopy analysis, Fourier Transform Infrared Spectrum Structure Profile characterisation, and in vitro biological effect of Salvia officinalis L. Phytocompounds

2020 ◽  
Vol 21 ◽  
Author(s):  
Issam Saidi ◽  
Fatma Guesmi ◽  
Ramzi Amari ◽  
Sana Ncib ◽  
Najla Hfaiedh
Keyword(s):  
Fourier Transform ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Fourier Transform Infrared ◽  
Salvia Officinalis ◽  
Bioactive Constituents ◽  
Antioxidant Power ◽  
Scanning Electron

Background: The aims of this research is to investigate the potential activity of Salvia officinalis and various hairs responsible of secretion of essential oil. In Tunisia, biological activity of Salvia officinalis is poorly recorded. Salvia leaves contain various types of hairs (glandular and non-glandular). Methods: The investigation of different trichomes were carried out by scanning electron microscope (SEM) apparatus. Antiradical potential were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. Antimicrobial activity was analysed using disc diffusion assay. The extracts of Salvia officinalis (SvOf), showed the following order of richness in phenolic contents: methanolic (70.76 mg GAE/g DW), aqueous (43.76 mg GAE/g DW) and infusion (9.42 mg GAE/g DW). The methanolic fraction records the highest levels of flavonoids (77 mg QE/g DW) compared with the aqueous extracts (33.19 mg EQ/g DW) and infusion (26.25 mg EQ/g DW). ME-SvOf showed higher value of free radical scavenging activity towards DPPH free radical and ferric reducing power. Results: The results revealed that main bioactive constituents in the methanolic fractions of sage leaves generally exhibited higher antibacterial effects. Overall, sage phytocompounds constitute a promising approach for the treatment of infectious diseases. Discussion: Functional groups detected in S. officinalis by Fourier transform infrared spectrophotometer (FT-IR) were mainly phenols, saccharides, amine and aromatic (Ar-H). Conclusion: Antiradical and antibacterial activities of Salvia officinalis are mainly due to phenolic content and other bioactive compounds. Non-glandular hairs are the most important trichomes in the Salvia leaves.

scholarly journals ANTIMICROBIAL AND FREE RADICAL SCAVENGING ACTIVITY OF LEAF AND STEM EXTRACT OF LIMONIA ALATA WIGHT AND ARN.

2018 ◽  
Vol 11 (4) ◽  
pp. 360
Author(s):  
Malathi S ◽  
Sekar T ◽  
Sujatha S
Keyword(s):  
Free Radical ◽  
Plant Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Metal Chelating

 Objective: In the present study, antioxidant activities leaves and stem of the petroleum, chloroform, acetone, and methanolic extracts from Limonia alata (L.). Leaf and stem were investigated by employing established in vitro studies. The leaves and bark are used for the fomentation of rheumatic pain; the dried fruit is useful in malignant and pestilent fevers and is used as an antidote for poisons, the folklore claim suggests that the leaf is showing wound healing property. L. alata is belonging to the family Rutaceae.Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2*−) scavenging activity, phosphomolybdenum assay, ferric reducing antioxidant power and metal chelating activity, was performed to know the antioxidant potency and antimicrobial studies of the plant extract of leaves and stem of L. alata.Result: Results are evaluated higher in leaves, stem and root of L. alata (L.) recorded total phenol (59.95±5.30). The present state of work was designed to evaluate the phytochemical, antioxidant as well as to screen the antimicrobial present in the plant extracts of L. alata. The plant L. alata petroleum ether extract of leaf showed greater IC50 for DPPH assay (154.8 μg/mL) and compare to another extract, higher reducing power activity stem in methanol (0.99EDTAE/100 g extract), better phosphomolybdenum reduction (0.512 mg/g extract) higher ferric reducing power (11542.3MmolFe(II)E/mg) extract and higher superoxide radical scavenging activity in leaf extract (332.13%). However, the better metal chelating ability was shown by the water extracts of the leaf (18.24 EDTAE/100 g) compared to other solvent extracts.Conclusion: The result indicates the antioxidant and antibacterial activity potential of L. alata.

EVALUATION OF FREE RADICAL SCAVENGING POTENTIAL OF 7-HYDROXY–4-METHYLCOUMARIN ISOLATED FROM MIMOSA NILOTICA (L)

2021 ◽  
pp. 10-12
Author(s):  
S Srinivasan ◽  
A Rayar
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavengers ◽  
Bioactive Constituents

Introduction:Medicinal plants have been used for the treatment of diseases all over the world before the advent of articial clinical drugs and are known to contain substances that can be used for therapeutic uses. Antioxidations act asreducing agents, free radical scavengers, quenchers of singlet oxygen molecule, and activators for antioxidative enzyme to reduce the damage caused by free radicals in biological pattern. Materials and Methods:The isolated organic compound from Mimosa nilotica was subjected to column chromatographic separation and was characterized 1 13 by UV, IR, H-NMR and C-NMR.DPPH assay was conducted to assess the effect of the ethanolic extract and 7-hydroxy–4-methylcoumarin. Result and Discussion:The reports of the in-vitro antioxidation assay indicated that the ethanol extract was more potent than 7-hydroxy–4- methylcoumarin. 7-hydroxy–4-methylcoumarin and ethanolic extractexpress excellent free radical scavenging activity. From the reports it is proved that the reducing power of bioactive constituent is associated with antioxidationpotential. Thus a correlation is proved between reducing power and the antioxidation effect. Conclusion:Previous reports are conrmed that the reducing power of bioactive constituents is associated with antioxidation potential. People who eat various parts of fruits and herbs have a lower risk of heart disease and some neurological diseases and there is evidence that some types of herbs, and fruits in general, protect against some malignant.

scholarly journals Antioxidant and radical scavenging activities of some norcantharidin and bridged perhydroisoindole derivatives

2013 ◽  
Vol 78 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Aysegul Peksel ◽  
Cumali Celik ◽  
Nuket Ocal ◽  
Refiye Yanardag
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Antioxidant Power

A series of norcantharidin and bridged perhydroisoindole derivatives were evaluated for their antioxidant and radical scavenging activities. Different in vitro methodologies such as total reducing power, 1,1-diphenyl-2- picrylhydrazil (DPPH?) free radical scavenging, superoxide anion radical scavenging and metal chelating activities were used. Among 11 compounds tested, 7 compounds showed potent reducing power activity and 8 compounds showed potent superoxide anion radical scavenging activity. All the tested compounds exhibited potent free radical scavenging ability. The results showed that the synthesized compounds had effective antioxidant power.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

scholarly journals Aktivitas Antioksidan Minuman Daun Beluntas Teh Hitam (Pluchea indica Less-Camelia sinensis)

2018 ◽  
Vol 38 (2) ◽  
pp. 200
Author(s):  
Paini Sri Widyawati ◽  
Tarsisius Dwi Wibawa Budianta ◽  
Yesiana Dwi Wahyu Werdani ◽  
Maria Olivia Halim
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Black Tea ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Iron Ion

The research was conducted to explore the potency of pluchea leaves-black tea drink as antioxidant at various proportions. The research used a single factor randomized block design of pluchea leaves and black tea proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w). Each of it was repeated five times. The parameters observed in this study were secondary metabolites, total phenolic, total flavonoids, free radical DPPH scavenging activity, and iron reducing power. The data were statistically analyzed using Analysis of Variance (ANOVA) at α = 5%, if the analysis showed a significant effect then it was continued with Duncan's Multiple Range Test (DMRT). Data was stated as mean ± standard deviation. The results showed that the secondary metabolites containing in drink from pluchea leaves and black tea at various proportions were alkaloids, flavonoids, phenolics, saponins, tannins, and cardiac glycosides. The increasing of black tea proportion in samples added the intensity of alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides compounds detected, but the tannins were decreased. These secondary metabolites were correlated with total phenolic content (TPC) and total flavonoid content (TFC). The increasing of black tea proportion in drink significantly decreased DPPH free radical scavenging activity and iron ion reducing power, except for 100% black tea proportion. Tannin compounds seems determining antioxidant activity.  Based on coefficient correlation between TPC or TFC and DPPH free radical scavenging activity or iron ion reducing power, the result showed that DPPH free radical scavenging activity was dominantly contributed by TPC and iron ion reducing power was determined by TPC and TFC. ABSTRAKPenelitian ini dilakukan untuk mengetahui potensi minuman daun beluntas teh hitam sebagai antioksidan pada berbagai proporsi. Penelitian ini menggunakan rancangan acak kelompok satu faktor, yaitu proporsi daun beluntas teh hitam meliputi 100:0; 75:25; 50:50; 25:75; dan 0:100% (b/b). Tiap faktor diulang sebanyak 5 kali. Parameter yang diamati pada penelitian ini meliputi kandungan metabolit sekunder, total fenolik (TPC), total flavonoid (TFC), kemampuan menangkal radikal bebas DPPH, dan kemampuan mereduksi ion besi. Data dianalisis secara statistik dengan Analysis of Variance (ANOVA) pada α = 5%, jika terdapat beda signifikan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Data dinyatakan dengan rata-rata ± standar deviasi.  Hasil menunjukkan bahwa metabolit sekunder yang terkandung dalam minuman daun beluntas teh hitam pada berbagai proporsi adalah alkaloid, flavonoid, fenolik, saponin, tannin, dan kardiak glikosida. Peningkatan proporsi teh hitam menambah intensitas senyawa alkaloid, fenolik, flavonoid, saponin, dan kardiak glikosida yang terdeteksi, tetapi kandungan senyawa tannin berkurang. Kandungan metabolit sekunder ini berkorelasi dengan total fenolik (TPC) dan total flavonoid (TFC). Peningkatan proporsi teh hitam dalam minuman menurunkan kemampuan menangkal radikal bebas DPPH dan kemampuan mereduksi ion besi, kecuali pada proporsi teh hitam 100%. Kandungan senyawa tannin dalam minuman menentukan aktivitas antioksidan. Berdasarkan koefisien korelasi antara TPC atau TFC dan kemampuan menangkal radikal bebas DPPH atau kemampuan mereduksi ion besi, hasil menunjukkan bahwa kemampuan menangkal radikal bebas DPPH dominan dikontribusi oleh TPC dan kemampuan mereduksi ion besi ditentukan oleh TPC dan TFC.

scholarly journals Effect of the Solvent on Propolis Phenolic Profile and its Antifungal, Antioxidant, and In Vitro Cytoprotective Activity in Human Erythrocytes Under Oxidative Stress

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4266
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Patrycja Kwaśniewska-Sip ◽  
Agnieszka Waśkiewicz ◽  
Piotr Nowak ◽  
...  
Keyword(s):  
Free Radical ◽  
Antifungal Activity ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Phenolic Profile ◽  
Cytoprotective Activity

Propolis is a natural bee product with various beneficial biological effects. The health-promoting properties of propolis depend on its chemical composition, particularly the presence of phenolic compounds. The aim of this study was to evaluate the relationship between extraction solvent (acetone 100%, ethanol 70% and 96%) and the antifungal, antioxidant, and cytoprotective activity of the extracts obtained from propolis. Concentrations of flavonoids and phenolic acids in the propolis extracts were determined using ultrahigh-performance liquid chromatography. The antioxidant potential of different extracts was assessed on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free-radical-scavenging activity, Fe3+-reducing power, and ferrous ion (Fe2+)-chelating activity assays. The ability of the extracts to protect human red blood cell membranes against free-radical-induced damage and their antifungal activity was also determined. The results showed that the concentration of flavonoids in the propolis extracts was dependent on the solvent used in the extraction process and pinocembrin, chrysin, galangin, and coumaric acid were the most abundant phenols. All extracts exhibited high antioxidant potential and significantly protected human erythrocytes against oxidative damage. On the other hand, the antifungal activity of the propolis extracts depended on the solvent used in extraction and the fungal strains tested. It needs to be stressed that, to the best of our knowledge, there is no study relating the effect of solvent used for extraction of Polish propolis to its phenolic profile, and its antifungal, antioxidant, and cytoprotective activity.

scholarly journals Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints

2020 ◽  
Vol 21 (9) ◽  
pp. 3087 ◽  
Author(s):  
Katarína Ražná ◽  
Zuzanna Sawinska ◽  
Eva Ivanišová ◽  
Nenad Vukovic ◽  
Margarita Terentjeva ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Minimal Inhibition Concentration ◽  
Fresh Matter ◽  
Antioxidant Power

The aim of this study was to characterize extracts from the leaves of Ginkgo biloba L. from selected Slovakian localities in terms of the content of bioactive constituents, antioxidants and their antimicrobial properties. The results indicated that the content of antioxidants was sample-specific, and this specificity was statistically significant. Ginkgo biloba L. from the locality of Košice had the best activity determined by the free radical scavenging activity (DPPH) (1.545 mg Trolox equivalent antioxidant capacity (TEAC)/g fresh matter (FM)) as well as the molybdenum-reducing antioxidant power (35.485 mg TEAC/g FM) methods. The highest content of total polyphenols (2.803 mg gallic acid equivalent (GAE)/g FM) and flavonoids (4.649 μg quercetin equivalent (QE)/g FM) was also detected in this sample. All samples of G. biloba leaf extracts showed significant antimicrobial activity against one or more of the examined bacterial species, and Staphylococcus aureus subsp. aureus CCM 2461 was found to be the most susceptible (minimal inhibition concentration MIC50 and MIC90 values of 64.2 and 72.2 µg/mL, respectively). Based on the results it was concluded that Ginkgo biloba L. extracts can be used as antimicrobial and antioxidant additives. Selected miRNA-based molecular markers were used to examine the environmental adaptability of Ginkgo biloba L. An almost-complete genotype clustering pattern based on locality was determined in the analysis that involved a species-specific gb-miR5261 marker. Morphologically specific exemplar, cv. Ohatsuki, was excluded.

scholarly journals Free Radical Scavenging Activity, Reducing Power and Anti-Hemolytic Capacity of Algerian Drimia maritima Baker Flower Extracts

2020 ◽  
Vol 10 (4) ◽  
pp. 70-78
Author(s):  
Abir Rezzagui ◽  
Abderrahmane Senator ◽  
Soumia Benbrinis ◽  
Hamama Bouriche
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Superoxide Anion ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity

This study was undertaken to evaluate the antioxidant and anti-hemolytic properties of Algerian Drimia maritima Baker flower extracts. Determination of phenolic content was carried out to estimate the chemical composition of D. maritima extracts. Antioxidant properties were investigated in all extracts using free radical scavenging activity (against DPPH, ABTS, hydroxyl radical, and superoxide anion), reducing power, inhibition of lipid peroxidation, and anti-hemolytic capacity. Phenolic determination revealed that D. maritima flowers contain phenolic compounds, flavonoids, and tannins. Ethyl acetate extract showed the highest reducing power and scavenging activity using DPPH and ABTS assays. However, aqueous extract was the most effective against hydroxyl radical, superoxide anion, and lipid peroxidation. The half-time of hemolysis indicates that chloroform extract exhibited the best anti-hemolytic capacity in the AAPH induced hemolysis model. The results of this study suggest that D. maritima could be used as a possible source of antioxidant phenolic compounds and that further determination of these compounds may provide more information on their medicinal value.  Keywords: Drimia maritima, phenolic compounds, scavenging activity, reducing power, anti-hemolytic.

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