Effects of Fermentative Factors on Biochemical, Microbiological and Sensory Characteristics of Probiotic Iranian Fermented Milk (Doogh)

2019 ◽  
Vol 15 (1) ◽  
pp. 40-47
Author(s):  
Elaheh Ahmadi ◽  
Reza Mohammadi ◽  
Sara Hasanvand ◽  
Milad Rouhi ◽  
Amir Mohammad Mortazavian ◽  
...  
Keyword(s):  
Incubation Temperature ◽  
Storage Temperature ◽  
Starter Culture ◽  
Fermented Milk ◽  
Biochemical Properties ◽  
Viable Count ◽  
Sensory Characteristics ◽  
Refrigerated Storage ◽  
Increase Rate ◽  
Probiotic Strains

Background: Doogh is a traditional Iranian fermented milk drink that is currently a very popular product in Iran. In the present study, the combined effects of incubation temperature, type of starter culture, and final pH of fermentation on the viability of two probiotic strains (Lactobacillus acidophilus La-5 (A) and Bifidobacterium lactis Bb-12 (B)) and biochemical and sensory characteristics of probiotic Doogh were investigated. </P><P> Method: Different treatments were prepared with yoghurt starter culture containing probiotics, using reconstituted skimmed milk with 6% of solid nonfat. They were incubated at 38°C or 44°C until pH 4.0 or pH 4.5 and were kept for 21 days at 4&#176;C or 8&#176;C storage temperature. The biochemical properties of treatments were evaluated during fermentation and refrigerated storage. The viable count and sensory attributes of treatments were studied at the end of fermentation and at the end of storage. </P><P> Results: Results showed that the greatest mean pH drop rate, mean acidity increase rate and mean redox potential increase rate and the shortest incubation time were observed in treatments incubated at 44&#176;C with final pH of 4.5. The highest survival of both probiotic strains during storage was related to treatments with fermentation temperature of 38&#176;C and final pH of 4.5. Treatments ABY1-38&#176;C-4.0 had the highest sensorial total acceptance at the end of refrigerated storage. </P><P> Conclusion: It would be concluded that the best treatment was ABY3-38&#176;C-4 stored at 8°C in regards of probiotics’ viability and sensory characteristics of Doogh. Thus, controlling the temperature of refrigerated storage is the most importance factor during storage and distribution.

scholarly journals Detection of cheese whey and caseinomacropeptide in fermented milk beverages using high performance liquid chromatography

2014 ◽  
Vol 66 (3) ◽  
pp. 959-964 ◽  
Author(s):  
E.H.P. Andrade ◽  
M.R. Souza ◽  
L.M. Fonseca ◽  
C.F.A.M. Penna ◽  
M.M.O.P. Cerqueira ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Storage Time ◽  
Cheese Whey ◽  
Starter Culture ◽  
Gel Filtration ◽  
Fermented Milk ◽  
Refrigerated Storage ◽  
Four Levels

Cheese whey level and caseinomacropeptide (CMP) index of fermented milk beverages added with four levels of cheese whey (0, 10, 20, and 40%) and stored at 8-10oC for 0, 7, 14 and 21 days were determined by high performance liquid chromatography-gel filtration (HPLC-GF). Additionally, the interference of the starter culture and the storage time on the detection of cheese whey and CMP were investigated. Refrigerated storage up to 21 days did not affect (P>0.05) cheese whey and CMP amounts in milk (0% of cheese whey) and in fermented milk beverages added with 10 and 20% of cheese whey (P>0.05). However, cheese whey and CMP amounts were higher than expected (P<0.05) in fermented milk beverages added with 40% of cheese whey and stored for 21 days.

Effectiveness of Soy Milk as Food Carrier for Lactobacillus Acidophilus

1993 ◽  
Vol 56 (4) ◽  
pp. 320-322 ◽  
Author(s):  
G. F. de VALDEZ ◽  
G. S. de GIORI
Keyword(s):  
Survival Rate ◽  
Lactobacillus Acidophilus ◽  
Acid Production ◽  
Starter Culture ◽  
Harmful Effect ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Refrigerated Storage ◽  
Soy Milk ◽  
Culture Activity

Three mild-fermented milk beverages prepared from soy milk and cow's milk were compared for their ability to preserve the cell viability of Lactobacillus acidophilus during refrigerated storage, in associative growth with Lactobacilus casei and Streptococcus thermophilus. The highest survival rate was obtained by using soy milk as substrate. The presence of L. casei in the starter culture had no influence on the viability of L. acidophilus, while the streptococcal cells showed a harmful effect. The culture activity measured as proteolysis and acid production remained fairly constant during the shelf life, despite the variations in colony counts observed for the different fermented milks analyzed.

scholarly journals Probiotic fermented milk drink added with cashew pulp (Anacardium occidentale): formulation, physicochemical, microbiological and sensory characteristics

2020 ◽  
Vol 9 (8) ◽  
pp. e990986753
Author(s):  
Flávia Roberta Monteiro de Souza ◽  
Natália Gabriela Silva Santos ◽  
Deborah de Melo Magalhães Padilha ◽  
Danielle Soares Bezerra ◽  
Maria Beatriz Mesquita Cansação Felipe ◽  
...  
Keyword(s):  
Storage Period ◽  
Fermented Milk ◽  
Sensory Characteristics ◽  
Refrigerated Storage ◽  
Milk Products ◽  
Fermented Beverages ◽  
Ph Values ◽  
Brazilian Legislation ◽  
Microbiological Stability ◽  
Fermented Milk Products

The health aspects associated with fermented milk products have resulted in increased consumption of this beverage. Three fermented beverages added with cashew pulp and different concentrations of whey (10, 20 and 30 %) were produced, being stored for a period of 28 days and evaluated regarding their physicochemical, microbiological and sensory characteristics. Fifty-two tasters evaluated acceptance of the beverages using hybrid 9-point hedonic scales. It was found that the lower the proportion of whey used in the beverages, the higher the total solids, proteins, fat and caloric values (p<0.05). Regarding ash values, no significant differences were observed (p>0.05). Beverages pH values did not differ when evaluated on the same day of storage (p>0.05). After 7, 14, 21 and 28 days of storage, differences were observed (p <0.05) regarding acidity values, except for the time 0. The beverages presented probiotic viability during the storage period, being the count above the minimum recommended by the current Brazilian legislation. Even though the beverages added with 10 % of whey presented a better acceptability index, the other beverages developed in this study presented good acceptance by the tasters. The probiotic beverages flavored with cashew fruit were nutritionally and technologically viable and presented physicochemical and microbiological stability during refrigerated storage for 28 days.

scholarly journals Effect of process variables on the physicochemical and rheological properties of yogurt

2021 ◽  
Vol 24 (1) ◽  
Author(s):  
Héctor J. Paz-Díaz ◽  
Lia Z. Mora-Vergara ◽  
Cristian E. Navarro-Arana ◽  
Alba Sofia Navarro ◽  
Mónica María Pacheco-Valderrama
Keyword(s):  
Incubation Temperature ◽  
Flow Behavior ◽  
Dairy Product ◽  
Starter Culture ◽  
Quality Criteria ◽  
Physicochemical Characteristics ◽  
Fermented Milk ◽  
Quality Parameters ◽  
Rheological Parameters ◽  
Fermentation Time

In this research, the effect of the lactic starter culture, the fermentation time, the incubation temperature and the percentage of fat on the firmness and consistency of a fermented milk drink was evaluated through physicochemical characteristics of total acidity, pH, syneresis and flow behavior. The purpose was to find the best formulation that extends the shelf life of yogurt, leading to an improvement in the manufacturing process of a local dairy company in Barrancabermeja (Santander, Colombia). Quality criteria were evaluated by means of the percentage of acidity and pH of the yogurt, as well as its syneresis and rheological parameters during a storage time of 24 hours and 7 days in refrigeration. Acceptable quality parameters were obtained for the yogurt prepared with an inoculum concentration of 2%, an incubation temperature of 40°C and an incubation time of 150 minutes. In addition, better properties of firmness and consistency were found, with absence of syneresis and viscosity in the accepted range, for this dairy product.

scholarly journals Effect of Pasteurization Temperature, Starter Culture, and Incubation Temperature on the Physicochemical Properties, Yield, Rheology, and Sensory Characteristics of Spreadable Goat Cheese

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Florencia Frau ◽  
Graciela Font de Valdez ◽  
Nora Pece
Keyword(s):  
Preparation Method ◽  
Incubation Temperature ◽  
Starter Culture ◽  
Starter Cultures ◽  
Sensory Characteristics ◽  
Processing Conditions ◽  
Goat Cheese ◽  
Autochthonous Starter Culture ◽  
The Impact

The local cheese factories currently use bovine commercial starter cultures, and the spreadable cheese process is not standardized. A detailed understanding of the effect of pasteurization temperature, starter culture, and incubation temperature must allow producers to optimize the process, increase cheese yield, and improve the quality of the final product. The main objective of the study was to describe the preparation method of spreadable goat cheese and investigate the effects of specific processing conditions (pasteurization temperature, starter culture, and incubation temperature) on the composition, yield, rheology, and sensory characteristics of the final product. Results showed the impact of pasteurization temperature, starter culture, and incubation temperature on spreadable goat cheese. The results indicated that it is possible to improve cheese yield, rheological properties, and sensory analysis when milk is pasteurized at 75°C for 30 minutes; cheese yield can also be improved by lowering incubation temperature to 37°C. Cheeses made with autochthonous starter culture showed better sensory characteristics and higher pH compared to the cheeses made with commercial culture.

scholarly journals The Functionality of African Streptococcus Thermophillus and Streptococcus Infantarius Sub Sp. Infantarius in Milk Fermentation

2021 ◽  
Vol 9 (1) ◽  
pp. 111-121
Author(s):  
Isaac M. Maitha ◽  
Dasel W. M. Kaindi ◽  
Cherotich Cheruiyot
Keyword(s):  
Dairy Products ◽  
Incubation Temperature ◽  
Ph Value ◽  
Starter Culture ◽  
Titratable Acidity ◽  
Fermented Milk ◽  
Camel Milk ◽  
Cow Milk ◽  
Milk Samples ◽  
Streptococcus Infantarius

Fermentation serves a key role in inhibiting spoilage microorganism through acidification and production of antimicrobial compounds. The technological information on properties of Streptococcus infantarius sub sp. infantarius which is predominant in most African fermented is dairy products very little. This study was therefore carried out to determine the functionality of selected African Streptococci strains in fermented dairy products. Pasteurized milk samples from camels and cows were inoculated with different strains and a selected combination at a rate of 3% v/v and incubated at different temperatures of 25 oC, 30 oC, 37 oC, and 45 oC for 9 hours. Analysis was done after every 3 hours for pH and titratable acidity while viscosity was done after incubation and cooling of the product. The different fermented milk samples were subsequently evaluated for consumer acceptability. Milk inoculated with both African type Streptococcus thermophillus (146A8.2) and Streptococcus infantarius sub sp. infantarius CJ 18 (9377), and incubated for nine hours recorded the highest amount of titratable acidity of 0.97 for the camel milk and had the least pH value of 4.12 for cow milk compared to the other strains. The cow milk had the highest viscosity level of 59.64 cPs compared to camel milk which was 29.44 cPs. The levels of titratable acidity and viscosity depended on the strain and incubation temperature. The African type Streptococcus thermophillus (146A8.2) and Streptococcus infantarius sub sp. infantarius CJ 18 (9377), isolated from fermented camel milk had good technological properties that are useful as starter culture for development of fermented milk products.

Effect of storage temperature on the chemical, microbiological, and sensory characteristics of pasta filata soft cheese containing probiotic lactobacilli

2019 ◽  
Vol 25 (7) ◽  
pp. 588-596 ◽  
Author(s):  
Facundo Cuffia ◽  
Yanina Pavón ◽  
Guillermo George ◽  
Jorge Reinheimer ◽  
Patricia Burns
Keyword(s):  
Storage Temperature ◽  
Lactobacillus Rhamnosus ◽  
Sensory Characteristics ◽  
Cold Chain ◽  
Lactobacillus Rhamnosus Gg ◽  
Soft Cheese ◽  
Probiotic Strains ◽  
Pasta Filata ◽  
Probiotic Lactobacilli ◽  
Selection Of

The aim of this study was to manufacture pasta filata cheeses added with two probiotic lactobacilli: Lactobacillus rhamnosus GG and Lactobacillus acidophilus LA5, either individually or combined, and to evaluate the effect of the storage temperature (4 and 12 °C) on their chemical, microbiological, and sensorial characteristics. Three cheese types were made: (i) G: containing L. rhamnosus GG, (ii) L: containing L. acidophilus LA5, and (iii) GL: containing both probiotic strains. Gross composition, pH, microbiological, and sensory characteristics were determined. No differences in gross composition were found among them. pH values remained above 5.2 in cheeses stored at 4 °C. However, a postacidification was observed in cheeses ripened at 12 °C. L. acidophilus LA5 was not able to grow, while L. rhamnosus GG grew 1.5 log10 CFU/g in G and GL cheeses stored at 12 °C, reducing the pH from day 8 onwards. These results emphasize the importance of the storage temperature since the good characteristics of probiotic cheeses are kept if the cold-chain is respected. Thus, the selection of probiotics, together with the food matrix and the starter, should be carefully evaluated.

Inhibition of Enteropathogenic Escherichia coli by Homofermentative Lactic Acid Bacteria in Skimmilk

1977 ◽  
Vol 40 (11) ◽  
pp. 749-753 ◽  
Author(s):  
J. F. FRANK ◽  
E. H. MARTH
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Incubation Temperature ◽  
Starter Culture ◽  
Fermented Milk ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Inhibition Of Growth ◽  
Generation Times ◽  
Present Generation

Behavior of enteropathogenic and nonpathogenic strains of Escherichia coli was determined when they were grown in skimmilk with and without 0.25 or 2.0% added lactic starter and incubated at 21 or 32 C for 15 h, and then after refrigeration at 7 C. With no lactics present, generation times for E. coli ranged from 28 to 35 min at 32 C and from 66 to 109 min at 21 C. At 32 C, after an initial 1- to 3-log increase in numbers and 6 to 9 h of incubation, E. coli was completely inhibited by both concentrations of starter culture. Complete inhibition of growth by E. coli occurred earlier at 32 than at 21 C, but smaller numbers of E. coli were obtained at 21 C; some strains virtually did not grow. The final pH of fermented milks ranged from 4.4 to 4.6. The longest survival time for E. coli in refrigerated fermented milk was about 17 days when milk was fermented at 32 C with 0.25% starter. A combination of lower incubation temperature (21 C) and higher starter concentration (2.0%) was most effective in controlling growth of E. coli in fermented skimmilk.

Physicochemical, microbiological and sensory profiles of fermented milk containing probiotic strains isolated from kefir

2011 ◽  
Vol 78 (4) ◽  
pp. 456-463 ◽  
Author(s):  
Emiliano Kakisu ◽  
Aurora Irigoyen ◽  
Paloma Torre ◽  
Graciela L De Antoni ◽  
Analía G Abraham
Keyword(s):  
Starter Culture ◽  
Streptococcus Thermophilus ◽  
Probiotic Strain ◽  
Fermented Milk ◽  
Sensory Attributes ◽  
Milk Product ◽  
Promising Alternative ◽  
Probiotic Strains ◽  
Viable Bacteria ◽  
Fermented Milk Product

A two-strain starter culture containing Lactobacillus plantarum CIDCA 83114, a potential probiotic strain isolated from kefir grains, and Streptococcus thermophilus CIDCA 321 was tested for the preparation of a fermented milk product. Kluyveromyces marxianus CIDCA 8154, a yeast with immunomodulatory properties was included to formulate a three-strain starter culture. Supernatants of enterohaemorragic Escherichia coli, shiga-toxin–producing strain, along with a two-strain or a three-strain starter culture were included in the medium of Vero-cell surface cultures. The results demonstrated that these combinations of microorganisms antagonize the cytopathic action of shiga toxins. The cell concentration of Lb. plantarum did not decrease during fermentation, indicating that the viability of this strain was not affected by low pH, nor did the number of viable bacteria change during 21 days of storage in either fermented products. The number of viable yeasts increases during fermentation and storage. Trained assessors analyzed the general acceptability of fresh fermented milks and considered both acceptable. The milk fermented with the two-strain starter culture was considered acceptable after two week of storage, while the product fermented with the three-strain starter culture remained acceptable for less than one week. The main changes in sensory attributes detected by the trained panel were in sour taste, milky taste and also in fermented attributes. The correlation between different sensory attributes and acceptability indicated that the panel was positively influenced by milky attributes (taste, odour, and flavour) as well as the intensity of flavour. In conclusion, the two-strain starter culture would be the more promising alternative for inclusion of that potential probiotic lactobacillus in a fermented milk product.

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