Biomarkers and Spectroscopic Methods: The Strategies for Diagnostics of Selected Diseases

2019 ◽  
Vol 13 (1) ◽  
pp. 8-18
Author(s):  
Kristína Krajčíková ◽  
Gabriela Glinská ◽  
Vladimíra Tomečková
Keyword(s):  
Body Fluid ◽  
Intestinal Ischemia ◽  
Spectroscopic Methods ◽  
Qualitative And Quantitative ◽  
Wide Range ◽  
Noninvasive Samples ◽  
Novel Biomarkers ◽  
Monitoring And Diagnostics ◽  
Qualitative And Quantitative Study

Background: There are many different tools for diagnostics of various diseases. One of the simplest approach for the early, rapid and accurate diagnosis represents determination of biomarkers. </P><P> Focus: In the following text, we describe review of the latest discoveries in the field of biomarkers of selected diseases: intestinal ischemia and atherosclerosis. The aim of this review article is to show the problems which the researchers have been dealing with in the process of discovering and establishing novel biomarkers. This work describes the possibilities of monitoring biomarkers from noninvasive samples such as tears. Additionally, the actual possibilities of the spectroscopy techniques in monitoring and diagnostics of selected diseases are mentioned which might replace the need of biomarkers of several diseases. </P><P> Prospect: For the most diagnostic purposes, biomarkers should be analyzed in body fluid samples. For the biofluids, metabolic signatures could be determined, although there is no consensus on possible biomarkers yet. Metabolomics, the comprehensive, qualitative, and quantitative study of secondary metabolites and signaling molecules reveal a wide range of dysregulated molecules in various diseases. However, using spectroscopic methods could contribute to the traditional view on biomarkers by monitoring the relevant tissues and body fluid samples.

Experimental Modeling and Electrochemical Analysis of Salted Oily Wastewaters

2015 ◽  
Vol 725-726 ◽  
pp. 1357-1362 ◽  
Author(s):  
Elizaveta Simonenko ◽  
Vyacheslav Starkov ◽  
Ludmila Molodkina ◽  
Alexander N. Chusov
Keyword(s):  
Specific Conductivity ◽  
Electrochemical Analysis ◽  
Model Systems ◽  
Buffer System ◽  
Combined Model ◽  
Qualitative And Quantitative ◽  
Wide Range ◽  
Quantitative Characteristics ◽  
Qualitative And Quantitative Characteristics

The results of potentiometric titration and determination of the specific conductivity of mineralized oily wastewater from landfill areaand the results of the analysis of individual and combined model systems (bicarbonate, phosphate and hydrogen sulphide) at different volume ratios and a wide range of mineral saltsconcentrations were compared. Qualitative and quantitative characteristics of studied wastewater buffer system were defined.

Simultaneous Determination of Prostaglandin and Hormones in Excreta of Trogopterus xanthipes

2020 ◽  
Vol 58 (6) ◽  
pp. 542-548
Author(s):  
Haolong Liu ◽  
Miaoxuan Fan ◽  
Xintong Fu ◽  
Yougen Chen ◽  
Min Ye ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Sensitivity ◽  
Chemical Profiling ◽  
Qualitative And Quantitative ◽  
Endogenous Prostaglandin ◽  
Wide Range ◽  
Quantitative Analyses ◽  
Good Linear Correlation

Abstract The excreta of Trogopterus xanthipes (also called Wulingzhi in Chinese, WLZ) is a well-known traditional Chinese medicine used for the treatment of irregular menstruation in clinic. Few reports are available on the chemical profiling of WLZ. In this work, qualitative and quantitative analyses of endogenous prostaglandin and hormones in WLZ were performed using UHPLC-orbitrap-MSn. In total, 48 compounds were identified in urine of T. xanthipes. Furthermore, the contents of four target compounds were simultaneously quantitated in 20 batches of samples by UPLC-MS/MS. The quantitative method showed a good linear correlation (R &gt; 0.995) in a wide range for each compound. The method had a high sensitivity with LOD (0.5–1.0 ng/mL) and LOQ (1.0–2.5 ng/mL). The intra- and inter-day precisions were &lt; 9.17 (RSD %), and repeatability and stability were &lt; 6.14 (RSD %). The recovery of the analytes varied between 85.8% and 97.3% at three different concentrations. The present integrated qualitative and quantitative assessment of WLZ provides an evaluation strategy to assess the constituent in traditional Chinese medicine.

scholarly journals A new method for quantitative and qualitative representation of the noises type in Allan (and related) variances

2020 ◽  
Vol 72 (1) ◽  
Author(s):  
Kamil Maciuk ◽  
Jacek Kudrys ◽  
Mohammad Bagherbandi ◽  
Igor V. Bezmenov
Keyword(s):  
Sampling Interval ◽  
Satellite Clock ◽  
Qualitative And Quantitative ◽  
Quantitative Expression ◽  
Noise Type ◽  
Wide Range ◽  
Averaging Time ◽  
The Stability ◽  
The Time Domain

AbstractAllan variances and its related methods are commonly used to analyse a sequence of data in the time domain to measure frequency stability of oscillators. It allows for a determination of noise type as a function of the averaging time. This method is one of the most popular for identifying different noise types in the sensors data, and it is usually used for a wide range of studies on the stability of oscillators or atomic clocks. In this paper, the authors propose their own algorithm for a qualitative and quantitative expression of noise type using Allan and related variation. The methods currently used allow for the determination of the noise type graphically only, without quantifying the type and percentages for each of the integration steps. Our study allows for a numerical interpretation of the Allan (and related) variances data. A numerical example of the described method is based on GPS satellite clock corrections for 1825–2086 GPS Week (5 years) based on daily 30-s sampling interval products.

Comparison of standardised lymphoscintigraphic function test and high resolution sonography of the lymphoedema of legs

Phlebologie ◽  
2008 ◽  
Vol 37 (05) ◽  
pp. 247-252 ◽  
Author(s):  
V. S. Brauer ◽  
W. J. Brauer
Keyword(s):  
Lower Leg ◽  
Clinical Findings ◽  
Lower Limbs ◽  
Fatty Tissue ◽  
Qualitative And Quantitative ◽  
Function Test ◽  
Unreliable Method ◽  
Negative Conclusion ◽  
Fine Disperse

SummaryPurpose: Comparison of qualitative and quantitative sonography with the lymphoscintigraphic function test and clinical findings in legs. Patients, methods: In 33 patients a lymphoscintigraphic function test of legs combined with measurement of lymph node uptake was performed and subsequently compared with sonography. Sonographic criteria were: Thickness of cutis, thickness of subcutanean fatty tissue and presence of liquid structures or fine disperse tissue structure of lower limbs, foots and toes. Results: In 51 legs uptake values lie in the pathologic area, in four legs in the grey area and in ten legs in the normal area. The cutis thickness in the lower leg shows no significant correlation with the uptake. The determination of the thickness of the subcutanean fatty tissue of the lower leg and of the cutis thickness of the feet turned out to be an unreliable method. In 47% of the medial lower legs and in 57% of the lateral lower legs with clinical lymphoedema sonography is falsely negative. Conclusion: Early lymphoedema is only detectable with the lymphoscintigraphic function test. In the case of clinical lymphoedema clinical examination is more reliable than sonography.

DINAMIKA POLITIK PERSETUJUAN BERSAMA TENTANG PENETAPAN DESA ADAT DI KABUPATEN ROKAN HULU TAHUN 2014

2017 ◽  
Vol 15 (1) ◽  
pp. 21
Author(s):  
Haryo Suganda ◽  
Raja Muhammad Amin
Keyword(s):  
Regulatory Region ◽  
Field Research ◽  
Analysis Techniques ◽  
Political Dynamics ◽  
Political Interests ◽  
Wide Range ◽  
Information Method ◽  
Qualitative Descriptive ◽  
The Village

This study is motivated the identification of policies issued by the regional Governmentof Rokan Hulu in the form of Regulatory region number 1 by 2015 on the determination of thevillage and Indigenous Village. Political dynamics based on various interests against themanufacture of, and decision-making in the process of formation of the corresponding localregulations determination of Indigenous Villages in the Rokan Hulu is impacted to a verysignificantamount of changes from the initial draft of the number i.e. 21 (twenty one) the villagebecame Customary 89 (eighty-nine) the Indigenous Villages who have passed. Type of thisresearch is a qualitative descriptive data analysis techniques. The research aims to describe theState of the real situation in a systematic and accurate fact analysis unit or related research, aswell as observations of the field based on the data (information). Method of data collectionwas done with interviews, documentation, and observations through fieldwork (field research).The results of the research on the process of discussion of the draft local regulations andmutual agreement about Designation of Indigenous Villages in the Rokan Hulu is, showed thatthe political dynamics that occur due to the presence of various political interests, rejectionorally by Villagers who were judged to have met the requirements of Draft Regulations to beformulated and the area for the set to be Indigenous Villages, and also there is a desire fromsome villages in the yet to Draft local regulations in order to set the Indigenous village , there isa wide range of interests of these aspects influenced the agreement to assign the entire localVillage which is in the Rokan Hulu become Indigenous village, and the village of Transmigrationinto administrative Villages where the initiator of the changes in the number of IndigenousVillages in the Rokan Hulu it is the desire of the local Government of its own.

Qualitative and Quantitative Study of Verbal Behavior Style in Interviews with Patient's Companion in Family Medicine: Understanding and Measuring

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Turabian Jose Luis ◽  
Minier-Rodriguez Luis Enoc ◽  
Moreno-Ruiz Sandra ◽  
Rodriguez-Almonte Francis Eliant ◽  
Cucho-Jove Raul ◽  
...  
Keyword(s):  
Family Medicine ◽  
Verbal Behavior ◽  
Quantitative Study ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Study

The Comparative Study of Equisetum pratense, E. sylvaticum, E. telmateia: Accumulation of Silicon, Antioxidant and Antimicrobial Screening

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Previous Treatment ◽  
Total Phenolic ◽  
Quantitative Chemical Analysis ◽  
Qualitative And Quantitative ◽  
Antioxidant And Antimicrobial Activity ◽  
The Comparative Study

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).

A Qualitative and Quantitative Study of the Innervation of the Human Non Pregnant Uterus

2016 ◽  
Vol 18 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Silvia Tommaso ◽  
Carlo Cavallotti ◽  
Antonio Malvasi ◽  
Daniele Vergara ◽  
Antonio Rizzello ◽  
...  
Keyword(s):  
Quantitative Study ◽  
Pregnant Uterus ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Study

The protonation of the ethylenediphosphinetetraacetate anion and the crystal structure of the bis (hydrogen bromide) of ethylenediphosphinetetraacetic acid

1981 ◽  
Vol 46 (12) ◽  
pp. 3063-3073 ◽  
Author(s):  
Jana Podlahová ◽  
Bohumil Kratochvíl ◽  
Vratislav Langer ◽  
Josef Šilha ◽  
Jaroslav Podlaha
Keyword(s):  
Crystal Structure ◽  
Hydrogen Bonds ◽  
Structure Determination ◽  
Free Acid ◽  
Hydrogen Bromide ◽  
Carboxyl Groups ◽  
Spectroscopic Methods ◽  
X Ray ◽  
Partial Formation

The equilibria and mechanism of addition of protons to the ethylenediphosphinetetraacetate anion (L4-) were studied in solution by the UV, IR, 1H and 31P NMR spectroscopic methods. A total of six protons can be bonded to the anion. They are added stepwise, first with partial formation of zwitterions containing P-H bonds, which then dissociate with formation of the free acid, H4L, where all four protons are bonded in carboxyl groups. The formation of zwitterions is strongly dependent on the concentration. In the final stage, the acid bonds two additional protons to form the bis-phosphonium cation, H6L2+. A number of isostructural salts containing this cation, H4L.2 HX (X = Cl, Br, I), have been prepared. The X-ray crystal structure determination of the bromide confirmed the expected arrangement. The bromide crystals are monoclinic, a = 578.2, b = 1 425.0, c = 1 046.7 pm, β = 103.07° with a space group of P21/c, Z = 2. The final R factor was 0.059 based on 1 109 observed reflections. The structure consists of H6L2+ cations containing protons bonded to phosphorus atoms (P-H distance 134 pm) and of bromide anions, located in gaps which are also sufficiently large for I- anions in the isostructural iodide. The interbonding of phosphonium cations proceeds through hydrogen bonds, C-OH...O=C, in which the O...O distance is 275.3 pm.

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