Antimicrobial Activity of Biosynthesized Metal Nanoparticles

2020 ◽  
Vol 10 (1) ◽  
pp. 20-35
Author(s):  
Tina Nasrin ◽  
Parsha S. Karim ◽  
Soni Shaikh
Keyword(s):  
Metal Nanoparticles ◽  
Bulk Material ◽  
Nanoparticle Synthesis ◽  
Past Research ◽  
Drug Resistant ◽  
Biomedical Sciences ◽  
Self Defense ◽  
Distinctive Property ◽  
Pathogenic Microbes ◽  
Conventional Antibiotics

It has been well documented that microbes are able to create self-defense against conventional antibiotics. Such drug-resistant property of the microbes always inspired the researchers to develop an alternative strategy to control the growth of pathogenic microbes. Nanoparticles have received major importance because of their distinctive property over corresponding bulk material. For such unique property, from the recent past, research has been focused on the nanotechnology to uplift the biomedical sciences but hazardous byproducts of nanoparticle synthesis makes always retardation. In this review, we emphasized and elaborated the biosynthesis process of metal nanoparticles and how such particles can be considered for anti-microbial context.

Potential Antimicrobial Peptides Elucidation From The Marine Bacteria

2020 ◽  
Vol 18 ◽  
Author(s):  
Adyasa Barik ◽  
Pandiyan Rajesh ◽  
Manthiram Malathi ◽  
Vellaisamy Balasubramanian
Keyword(s):  
Antimicrobial Peptide ◽  
Marine Bacteria ◽  
Unnatural Amino Acids ◽  
Wide Spectrum ◽  
Drug Resistant ◽  
Medical Field ◽  
The World ◽  
Pathogenic Microbes ◽  
Antimicrobial Peptide Resistance ◽  
Significant Attention

: In recent years, over use of antibiotics has been raising its head to a serious problem all around the world as pathogens become drug resistant and create challenges to the medical field. This failure of most potent antibiotics that kill pathogens increases the thirst for research to look further way of killing pathogens. It has been led to the findings of antimicrobial peptide which is the most potent peptide to destroy pathogens. This review gives special emphasis to the usage of marine bacteria and other microorganisms for antimicrobial peptide (AMP) which are eco friendly as well as a developing class of natural and synthetic peptides with a wide spectrum of targets to pathogenic microbes. Consequently, a significant attention has been paid mainly to (i) the structure and types of anti microbial peptides and (ii) mode of action and mechanism of antimicrobial peptide resistance to pathogens. In addition to this, the designing of AMPs has been analysed thoroughly for reducing toxicity and developing better potent AMP. It has been done by the modified unnatural amino acids by amidation to target the control of biofilm and persister cell.

Novel Compound from Flowers of Moringa oleifera Active Against Multi- Drug Resistant Gram-negative Bacilli

2020 ◽  
Vol 20 (1) ◽  
pp. 69-75
Author(s):  
Santi M. Mandal ◽  
Subhanil Chakraborty ◽  
Santanu Sahoo ◽  
Smritikona Pyne ◽  
Samaresh Ghosh ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Moringa Oleifera ◽  
Aqueous Acetic Acid ◽  
Phytochemical Analysis ◽  
Hydroxyl Groups ◽  
Drug Resistant ◽  
Gram Negative ◽  
Isolated Compound ◽  
Conventional Antibiotics

Background: The need for suitable antibacterial agents effective against Multi-drug resistant Gram-negative bacteria is acknowledged globally. The present study was designed to evaluate the possible antibacterial potential of an extracted compound from edible flowers of Moringa oleifera. Methods: Five different solvents were used for preparing dried flower extracts. The most effective extract was subjected to fractionation and further isolation of the active compound with the highest antibacterial effect was obtained using TLC, Column Chromatography and reverse phase- HPLC. Approaches were made for characterization of the isolated compound using FTIR, NMR and Mass spectrometry. Antibacterial activity was evaluated according to the CLSI guidelines. Results: One fraction of aqueous acetic acid extract of M. oleifera flower was found highly effective and more potent than conventional antibiotics of different classes against Multi-drug resistant Gram-negative bacilli (MDR-GNB) when compared. The phytochemical analysis of the isolated compound revealed the presence of hydrogen-bonded amine and hydroxyl groups attributable to unsaturated amides. Conclusion: The present study provided data indicating a potential for use of the flowers extract of M. oleifera in the fight against infections caused by lethal MDR-GNB. Recommendations: Aqueous acetic acid flower extract of M. oleifera is effective, in-vitro, against Gram-negative bacilli. This finding may open a scope in pharmaceutics for the development of new classes of antibiotics.

A quest to the therapeutic arsenal: Novel strategies to combat multidrug-resistant bacteria

2021 ◽  
Vol 21 ◽  
Author(s):  
Priyanka Ashwath ◽  
Akhila Dharnappa Sannejal
Keyword(s):  
Multidrug Resistant ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antimicrobial Drugs ◽  
Short Palindromic Repeat ◽  
Prevention Of Infections ◽  
Resistant Bacterial Strain ◽  
Conventional Antibiotics ◽  
Drug Resistant Strains

: The increasing resistance of the disease-causing pathogens to antimicrobial drugs is a public health concern and a socio-economic burden. The emergence of multi-drug resistant strains has made it harder to treat and combat infectious diseases with available conventional antibiotics. There are currently few effective therapeutic regimens for the successful prevention of infections caused by drug-resistant microbes. The various alternative strategies used in the recent past to decrease and limit antibiotic resistance in pathogens include bacteriophages, vaccines, anti-biofilm peptides, and antimicrobial peptides. However, in this review, we focus on the novel and robust molecular approach of antisense RNA (asRNA) technology and the clustered regulatory interspaced short palindromic repeat (CRISPR)-based antibiotic therapy, which can be exploited to selectively eradicate the drug-resistant bacterial strain in a sequence-specific fashion establishing opportunities in the treatment of multi-drug resistant related infections.

scholarly journals Two distinct amphipathic peptide antibiotics with systemic efficacy

2020 ◽  
Vol 117 (32) ◽  
pp. 19446-19454 ◽  
Author(s):  
Jayaram Lakshmaiah Narayana ◽  
Biswajit Mishra ◽  
Tamara Lushnikova ◽  
Qianhui Wu ◽  
Yashpal S. Chhonker ◽  
...  
Keyword(s):  
Spiral Structure ◽  
Drug Resistant ◽  
Peptide Antibiotics ◽  
Murine Models ◽  
Amphipathic Peptide ◽  
Antibiotic Resistant ◽  
Guided Discovery ◽  
Nmr Data ◽  
Conventional Antibiotics ◽  
Topical Applications

Antimicrobial peptides are important candidates for developing new classes of antibiotics because of their potency against antibiotic-resistant pathogens. Current research focuses on topical applications and it is unclear how to design peptides with systemic efficacy. To address this problem, we designed two potent peptides by combining database-guided discovery with structure-based design. When bound to membranes, these two short peptides with an identical amino acid composition can adopt two distinct amphipathic structures: A classic horizontal helix (horine) and a novel vertical spiral structure (verine). Their horizontal and vertical orientations on membranes were determined by solid-state15N NMR data. While horine was potent primarily against gram-positive pathogens, verine showed broad-spectrum antimicrobial activity. Both peptides protected greater than 80% mice from infection-caused deaths. Moreover, horine and verine also displayed significant systemic efficacy in different murine models comparable to conventional antibiotics. In addition, they could eliminate resistant pathogens and preformed biofilms. Significantly, the peptides showed no nephrotoxicity to mice after intraperitoneal or intravenous administration for 1 wk. Our study underscores the significance of horine and verine in fighting drug-resistant pathogens.

scholarly journals Treating Polymicrobial Infections in Chronic Diabetic Wounds

2019 ◽  
Vol 32 (2) ◽  
Author(s):  
Pranali J. Buch ◽  
Yunrong Chai ◽  
Edgar D. Goluch
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Genetic Diseases ◽  
Nanoparticle Synthesis ◽  
Delivery Systems ◽  
Bacterial Biofilms ◽  
Conventional Antibiotics ◽  
Diabetic Wounds ◽  
Polymicrobial Infections ◽  
Made In

SUMMARY This review provides a comprehensive summary of issues associated with treating polyclonal bacterial biofilms in chronic diabetic wounds. We use this as a foundation and discuss the alternatives to conventional antibiotics and the emerging need for suitable drug delivery systems. In recent years, extraordinary advances have been made in the field of nanoparticle synthesis and packaging. However, these systems have not been incorporated into the clinic for treatments other than for cancer or severe genetic diseases. We present a unifying perspective on how the field is evolving and the need for an early amalgamation of engineering principles and a biological understanding of underlying phenomena in order to develop a therapy that is translatable to the clinic in a shorter time.

scholarly journals Nanoparticles: Alternatives Against Drug-Resistant Pathogenic Microbes

Molecules ◽  
2016 ◽  
Vol 21 (7) ◽  
pp. 836 ◽  
Author(s):  
Gudepalya Rudramurthy ◽  
Mallappa Swamy ◽  
Uma Sinniah ◽  
Ali Ghasemzadeh
Keyword(s):  
Drug Resistant ◽  
Pathogenic Microbes

scholarly journals A3, a Scorpion Venom Derived Peptide Analogue with Potent Antimicrobial and Antibiofilm Activity against Clinical Isolates of Multi-Drug Resistant Gram Positive Bacteria

2018 ◽  
Author(s):  
Ammar Almaaytah ◽  
Ahmad Farajallah ◽  
Ahmad Abualhaijaa ◽  
Qosay Al-balas
Keyword(s):  
Host Defense ◽  
Mammalian Cells ◽  
Scorpion Venom ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Gram Positive ◽  
Peptide Analogue ◽  
Gram Positive Bacteria ◽  
Conventional Antibiotics

Current research in the field of antimicrobials is focused on the development of novel antibiotics and antimicrobial agents to counteract the huge dilemma that the human population is mainly facing in regards to the rise of bacterial resistance and biofilm infections. Host Defense peptides (HDPs) are a promising group of molecules for antimicrobial development as they share unique characteristics suitable for antimicrobial activity including their broad spectrum of activity and potency against bacteria. AamAP1 is a novel HDP that was identified through molecular cloning from the venom of the North African scorpion Androctonus amoeruxi. In vitro antimicrobial assays revealed that the peptide displays moderate activity against different strains of Gram-positive and Gram-negative bacteria. Additionally, the peptide proved to be highly hemolytic and displaying significantly high toxicity against mammalian cells. In our study, a novel synthetic peptide analogue named A3 was designed from the naturally occurring scorpion venom host defense peptide. The design strategy depended on modifying the amino acid sequence of the parent peptide in order to increase its net positive charge, percentage helicity and optimize other physico-chemical parameters involved theoretically in HDPs activity. Accordingly, A3 was evaluated for its in vitro antimicrobial and anti-biofilm activity individually and in combination with four different types of conventional antibiotics against clinical isolates of multi-drug resistant (MDR) Gram-positive bacteria. A3 was also evaluated for its cytotoxicity against mammalian cells. A3 displayed potent and selective in vitro antimicrobial activities against a wide range of MDR Gram-positive bacteria. Our results also showed that combining A3 with conventional antibiotics displayed a synergistic mode of action which resulted in decreasing the MIC value for A3 peptide as low as 0.125 µM. These effective concentrations were associated with negligible toxicities on mammalian cells. In conclusion, A3 exhibits enhanced activity and selectivity when compared with the parent natural scorpion venom peptide. The combination of A3 with conventional antibiotics may be pursued as a potential novel treatment strategy against MDR and biofilm forming bacteria.

scholarly journals Plant Extract Mediated Synthesis of Transition Metal Nanoparticles: A Review

2021 ◽  
Vol 9 (VI) ◽  
pp. 1989-1995
Author(s):  
Nimish Kumar
Keyword(s):  
Transition Metal ◽  
Metal Nanoparticles ◽  
Plant Extract ◽  
Nanoparticle Synthesis ◽  
Green Technology ◽  
Synthesis Methods ◽  
Transition Metal Nanoparticles ◽  
Plant Parts ◽  
Wide Range ◽  
Potential Applications

Green technology is a fast evolving scientific topic that has attracted a lot of attention in recent years due to its wide range of applications. It is a multidisciplinary field that is safe, non-hazardous, and ecologically friendly, in contrast to chemical and physical approaches for nanoparticle synthesis. Because the existing biomolecules in plant extract act as both a reducing and capping agent, the produced nanoparticles are very stable. As a result, nanoparticles that have been manufactured have a wide range of potential applications in the environmental and biomedical domains. The current report contains current information on numerous green synthesis methods that rely on different plant parts for green transition metal nanoparticles synthesis.

scholarly journals Modelling the Transitional Dynamics of Mycobacterium Tuberculosis Strain

2016 ◽  
Vol 5 (2) ◽  
pp. 13-23
Author(s):  
A. Alhassan ◽  
K.S. Nokoe
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Markov Chain Model ◽  
Acquired Resistance ◽  
World Health ◽  
Chain Model ◽  
Drug Resistant ◽  
Transitional Dynamics ◽  
Biomedical Sciences ◽  
First Line ◽  
Discrete State

The World Health Organization’s targets of eliminating Tuberculosis (TB) by 2050 is challenged by the emergence and spread of drug resistance TB. However, the traditional mechanism of resistance is that of acquired resistance, whereby the mycobacterium Tuberculosis (MTB) strain develops mutations under selective pressure of insufficient drug therapy. These mutations have the tendency of changing the drug target protein, restricting the bacteria to the anti-TB agent. We propose a discrete state markov chain model with three disease states: Drug Susceptible (DS), Multi Drug Resistant (MDR) and Extra Drug Resistant (XDR) to further study the transitional dynamics of the MTB strain. The study made use of a retrospective data on resistant pattern to first line and second line anti TB drugs. The structural properties of the model established life expectancies of DS and MDR strains as well as the probability of first resistance of the DS strain. Key estimates were assessed by the bootstrapping procedure which converged in estimates to the actual data. If the experiment were repeated infinitely many times, in 95 out of 100, the interval 2.782 x 10-7 to 0.018 will contain the true probability of first mutation of the DS strain. A key contribution of this study is the revealing therapeutic cycle of the treatment regime of the TB disease based on the TB progression data which saw the period after the 20th cycle of the treatment being prominent in some key strain dynamics. These findings may also help explain further the pharmacodynamic properties of the "first line" anti-Tuberculosis drugs for enhance TB treatment. Journal of Medical and Biomedical Sciences (2016) 5(2), 13-23

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