Laser biostimulation for improving seeds germinative capacity and seedlings growth of Prosopis laevigata and Jacaranda mimosifolia

“Jacaranda” (Jacaranda mimosifolia) and "mezquite” (Prosopis laevigata) are timber species that generally present problems with regard to the germination of their seeds in natural conditions, since they have a very hard and impermeable cover. Different treatments have been applied to improve the germinative response of both species with unfavorable results. This study aimed to know the effect of the pre-sowing laser biostimulation on germination and seedlings growth of these species. The seeds were biostimulated with a He-Ne laser (632 nm, 10 mW). Considering three replications of 50 seeds for each species, five irradiation treatments were applied (30 s, 60 s, 90 s, 120 s, 150 s) and control. The results for mezquite demonstrated that the highest number of germinated seeds (96%) was obtained with the treatments 90 s and 150 s; in contrast, the control seeds showed a lower germination percentage (16%). Additionally, the 30 s treatment produced a positive effect on the growth of the root, and it was different (p < 0.05) to the remaining treatments and the control. For Jacaranda, there were significant statistical differences (p < 0.05) between the control and the different treatments. 29% of seed germination occurs without any treatment. However, for 90 s and 120 s, germination is induced in a relatively high percent (97%-99%). For both species, the best treatment was 120 s, since higher values were recorded for all the morphological variables. It is concluded that laser biostimulation can produce beneficial effects on the germination of seeds and seedling growth and it could contribute to the propagation and conservation of these species.

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ENHANCING SEED GERMINATION OF FOUR CROP SPECIES USING AN ULTRASONIC TECHNIQUE

Experimental Agriculture ◽

10.1017/s0014479709991062 ◽

2010 ◽

Vol 46 (2) ◽

pp. 231-242 ◽

Cited By ~ 33

Author(s):

S. J. GOUSSOUS ◽

N. H. SAMARAH ◽

A. M. ALQUDAH ◽

M. O. OTHMAN

Keyword(s):

Seed Germination ◽

Treatment Duration ◽

Germination Rate ◽

Germination Percentage ◽

Crop Species ◽

Maximum Increase ◽

Beneficial Effects ◽

The Us ◽

Two Factors ◽

Parallel Tests

SUMMARYA laboratory experiment was conducted to determine the effect of ultrasound (US) treatment on seed germination of chickpea, wheat, pepper and watermelon. All tests were carried out at 40 kHz in a water bath ultrasonic device varying two factors, treatment duration (5, 10, 15, 30, 45 or 60 min) and germination temperature (15 or 20 °C). Parallel tests were run in which seeds were soaked in water without sonication in order to eliminate the effect of water from US test results. The effects of US on seed germination varied between crops and were more obvious on germination speed, expressed as germination rate index (GRI), rather than on germination percentage (GP). In particular, US treatment significantly increased the GRI of chickpeas, wheat and watermelon, resulting in a maximum increase of 133% (at 45 min), 95% (30 min) and 45% (5 min), respectively, above control seeds. The beneficial effects of US on the GRI of these crops were observed at both 15 and 20 °C, suggesting that US treatment offers a practical priming method to overcome the slow germination that may occur at low temperatures. Water-soaking treatment improved the GP of both chickpea and pepper seeds by 59 and 24%, respectively, compared to the control but neither water nor US had any positive effect on pepper GRI. Post-treatment measurement of moisture content of these seeds produced variable results depending on crop species and US treatment duration. Results of this research indicated that US treatment effectively enhanced speed of germination of chickpea, wheat and watermelon seeds. This increase in speed of germination may improve early field establishment of these crops in the semiarid Mediterranean region and thus needs further investigation. The US technique may also be very useful for plant propagators in nurseries to achieve fast seedling establishment of watermelon.

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Two-Hour Magneto-Priming with Static Magnetic Fields Ranging from 65 ± 3 to 505 ± 8 mT Does Not Improve the Germination Percentage of Industrial Hemp Seed at a Sub-Optimal Germination Temperature

J ◽

10.3390/j1010017 ◽

2018 ◽

Vol 1 (1) ◽

pp. 192-196

Author(s):

Kathrin Spendier

Keyword(s):

Seed Germination ◽

Magnetic Fields ◽

Cannabis Sativa ◽

Seed Priming ◽

Germination Percentage ◽

Hemp Seed ◽

Static Magnetic Fields ◽

Beneficial Effects ◽

Industrial Hemp ◽

The U.S

Industrial hemp is a non-psychoactive variety of Cannabis sativa L., i.e., it contains less than 0.3% tetrahydrocannabinols (THC). This crop is one of historical importance in the U.S. as manufacturers seek industrial hemp as a renewable and sustainable resource for a wide variety of consumer and industrial products. To help farmers succeed, agronomic research on industrial hemp is needed. In this trial, investigations were performed to determine whether magneto-priming, a form of seed priming that involves magnetic fields, effects the germination percentage of industrial hemp seed. Beneficial effects of magnetic fields on seedling growths and germination have been reported for many different plant species. Dry industrial hemp seed was exposed to static magnetic fields ranging from 65 ± 3 to 505 ± 8 mT for 2 h prior to seed germination. Germination was performed at 13.6 ± 0.7 °C, a temperature that is representative of the germination temperatures of industrial hemp in the U.S. state of Colorado. Magneto-priming of seed had no statistically significant effect on seed germination percentage.

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The effects of NACL, KCL and MGCL2 on the germination of Brassica rapa var. parachinensis seed

International Journal of Biological Research ◽

10.14419/ijbr.v4i1.5854 ◽

2016 ◽

Vol 4 (1) ◽

pp. 52 ◽

Cited By ~ 1

Author(s):

Mona Ghanad ◽

Rosimah Binit Nulit ◽

Rusea Go ◽

Christina Yong Seok Yien

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Seed Vigor ◽

Salinity Treatment ◽

Randomized Design ◽

Multiple Comparison Test ◽

Completely Randomized Design ◽

One Way Anova ◽

Range Test ◽

Germinated Seeds

This study was conducted to determine seed germination on the mechanisms of salinity stress in B. rapa var. parachinensis (Hong kong choy sum). The type of experiment was a completely randomized design with three salinity treatment NaCl, MgCl2, and KCl and minimum four replications was used. Salinity treatment consisted of 0 (control), 50, 100, 150, 200, mM concentrations. The experiment was carried out under 70% relative humidity at 23°C with a photoperiod of 8:16 h light/dark. The germinated seeds were counted every day and germination percentage, seed vigor, seed tolerance, length of seedling and biomass seedling was measured. The data were analyzed by one-way ANOVA multiple comparison test followed by Duncan’s multiple range test.

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SYNCHRONIZING THE in vitro GERMINATION OF Psidium guineense Sw. SEEDS BY MEANS OF OSMOTIC PRIMING

Revista Árvore ◽

10.1590/0100-67622016000400008 ◽

2016 ◽

Vol 40 (4) ◽

pp. 649-660 ◽

Cited By ~ 3

Author(s):

Márcia Adriana Carvalho dos Santos ◽

Mailson Monteiro do Rego ◽

Manoel Abilio de Queiroz ◽

Bárbara França Dantas ◽

Wagner Campos Otoni

Keyword(s):

Seed Germination ◽

Potassium Nitrate ◽

Germination Percentage ◽

In Vitro Germination ◽

Peg 6000 ◽

Caatinga Biome ◽

Germination Speed ◽

Mean Germination Time ◽

Positive Effect

ABSTRACT The Brazilian guava (Psidium guineense Swartz) is seed-propagated and, being native to the Caatinga biome, may frequently have uneven germination.Thus, we aimed to evaluate the synchronization of the in vitro seed germination of three accessions of the Brazilian guava, using water, polyethyleneglycol (PEG 6000), and potassium nitrate (KNO3) at different potentials and times of osmotic priming. Seeds from three accessions of the Brazilian guava (Y85, Y93,and Y97) from the UNEB/BA Germplasm Active Bank were subjected to the following pretreatments: -0.6, -1.0, -1.4, and -1,8 MPa PEG 6000; 10 and 20% KNO3 for 24h; 10 and 20% KNO3 for 48h; water for 24 and 48h; and non-primed seeds as the control. The experimental design was therefore a 10x3+1 factorial scheme. We assessed the germination percentage (G), mean germination time (MGT), germination speed (GS), and germination speed index (GSI). Data was subjected to analysis of variance followed by a means test (Duncan at 5% probability) and regression. There was interaction between the priming treatments and accessions for all evaluated features, except G. PEG 6000 decreased the MGT (from 6 to 8 days) and increased GS and GSI of seeds from all three accessions at potentials -1.0 to -1.5 MPa.Water-priming had a positive effect on MGT, GS, and GSI of accession Y85 seeds. KNO3 negatively affected germination of seeds from all three accessions. Thereby, we could synchronize seed germination of accessions Y85 and Y97 with PEG 6000.

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Effect of dew-irrigation on seed germination in flax, fenugreek, and fennel

Water Science & Technology Water Supply ◽

10.2166/ws.2019.166 ◽

2019 ◽

Vol 20 (1) ◽

pp. 269-276

Author(s):

Saiede Sargol Hosseini ◽

Hassan Heidari ◽

Mohammad Eghbal Ghobadi ◽

Iraj Nosratti

Keyword(s):

Seed Germination ◽

Water Availability ◽

Germination Percentage ◽

Cold Season ◽

Control Treatment ◽

Whole Plant ◽

The World ◽

Plant Stage ◽

Water Stage ◽

And Control

Abstract Drought limits plants growth. In many parts of the world, crop productions depend on water availability. Seed germination is a sensitive and low water stage in plants. A pot experiment was designed to test the effect of dew-irrigation on seed germination percentage of fennel, flax, and fenugreek. Irrigation treatments included dew-irrigation and control (non-irrigation). Results showed that dew-irrigation increased seed germination compared to control. Treatment of dew-irrigation had seed germination of 92.7%, 78.7% and 63.5% for flax, fenugreek, and fennel, respectively. But none of the seeds of control treatment germinated. Among plants studied, flax which is a cold season plant had the highest percentage of germination by dew-irrigation, and the plants that are the most heat-sensitive, such as fenugreek and fennel, had the lowest germination percentage. Results of this experiment determined that the moisture content in the air is capable to provide the necessary moisture for seed germination of the plants studied. In conclusion, dew-irrigation, due to supply low water, is better to be used at stages with lower water requirement such as germination. The ability of water supply by dew-irrigation at whole plant stage can be studied in the next experiments.

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An improved method of seed germination testing in Kabuli chickpea

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-4879 ◽

2018 ◽

Author(s):

Basave Gowda ◽

A. Krishna Naik ◽

Rakesh . ◽

C. Mathad ◽

B.S. Ganiger ◽

...

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Improved Method ◽

Testing Methods ◽

Seedling Vigour ◽

Seed Testing ◽

Testing Method ◽

Vigour Index ◽

Paper Method ◽

Germinated Seeds

An experiment was conducted during 2014-2015 to standardize the seed testing method in Kabuli chickpea MNK-1 by number of seeds and number of germination papers. Among the different testing methods 25 seeds per replication recorded highest seed germination percentage (91.58%), less number of fresh un-germinated seeds (1.91%), abnormal seedlings (4.00%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2215 and 6863), among the number of germination papers used 2+1 as in between paper method(Bottom 2 and one paper above) recorded significantly higher germination percentage (92.12%), less number of fresh un-germinated seeds (1.41%), abnormal seedlings (3.66%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2463 and 6900), when compared to other. Irrespective of number of germination paper and seeds used however, interaction of 25 seeds per replication and 2+1 between paper method of seed germination testing showed significantly germination percentage (96.50%), less number of fresh un-germinated seeds (0.00%), abnormal seedlings (1.00%), diseased seeds (1.00%) and significantly higher seedling vigour index-I and II (2844 and 7792) followed by 50 seeds per replication.

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Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

Agronomía Colombiana ◽

10.15446/agron.colomb.v33n3.53074 ◽

2016 ◽

Vol 34 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Fabio Ernesto Martínez M. ◽

Diego Miranda L. ◽

Stanislav Magnitskiy

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Positive Response ◽

Germination Rate ◽

Germination Percentage ◽

Annona Squamosa ◽

Germination Time ◽

Mean Germination Time ◽

Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

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Effect of salinity on seed germination of some tomato (Lycopersicon esculentum Mill.) varieties

Journal of Aridland Agriculture ◽

10.25081/jaa.2021.v7.6588 ◽

2021 ◽

pp. 76-82

Author(s):

Getachew Shumye Adilu ◽

Yohannes Gedamu Gebre

Keyword(s):

Seed Germination ◽

Abiotic Factors ◽

Germination Rate ◽

Petri Dish ◽

Germination Percentage ◽

Control Treatment ◽

Randomized Design ◽

Salinity Levels ◽

Mean Germination Time ◽

And Control

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.

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Seed Germination of Dirca (Leatherwood): Pretreatments and Interspecific Comparisons

HortScience ◽

10.21273/hortsci.40.6.1838 ◽

2005 ◽

Vol 40 (6) ◽

pp. 1838-1842 ◽

Cited By ~ 4

Author(s):

James A. Schrader ◽

William R. Graves

Keyword(s):

Seed Germination ◽

San Francisco ◽

Germination Percentage ◽

Eastern North America ◽

Cold Stratification ◽

Natural Conditions ◽

In The Wild ◽

Northeastern Mexico ◽

Understory Shrubs ◽

Interspecific Comparisons

The genus Dirca L. (Thymelaeaceae) consists of three species of understory shrubs. Dirca palustris L. is sparsely distributed across eastern North America, D. occidentalis Gray is endemic near the San Francisco Bay, and D. mexicana Nesom & Mayfield is known only in one population in northeastern Mexico. Despite interest in the horticultural use of Dirca, plants seldom are marketed. Difficult propagation impedes production of Dirca. We sought to define protocols that promote uniform seed germination of all three Dirca spp. Endodormancy and paradormancy cause sporadic germination over several years under natural conditions, but endocarp removal, cold stratification, and treatment with GA3 increased germination percentage, speed, and uniformity. Dirca occidentalis was most responsive; up to 94% of seeds germinated after endocarp removal, 24 hours in GA3 at 50 mg·L–1, and stratification at 4 °C for 30 days. Treatments also were effective for D. palustris (up to 68% germination), but seeds of D. mexicana were unresponsive and germinated at 25% or less. Seed treatments should facilitate production of D. occidentalis and D. palustris, but further research is needed to define methods to propagate D. mexicana for horticultural use and for conserving this rare species in the wild.

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Effects of different treatments on seed dormancy breaking and germination in Acer cappadocicum Gleditsch var. cappadocicum

Šumarski list ◽

10.31298/sl.144.3-4.5 ◽

2020 ◽

Vol 144 (3-4) ◽

pp. 159-166

Author(s):

Hanife Erdogan Genç ◽

Ali Ömer Üçler

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Germination Percentage ◽

Growth Chamber ◽

Dormancy Breaking ◽

Seed Collection ◽

Physiological Dormancy ◽

Germination Value ◽

Positive Effect ◽

Collection Time

This study was carried out to determine effects of different pretreatment on seed germination and to overcome dormancy in Acer cappadocicum seeds. The seeds were collected in 2008 three times with aproximately 15-days intervals. In order to overcome dormancy, several germination treatments were applied. The treatments were (1) different seed collection time, (2)soaking in water, (3) cold-moist stratification and (4) GA3 (gibberellic acid) application. The treated seeds were germinated in growing chamber at 5 0C and in greenhouse conditions. This research showed that seeds of Acer cappadocicum exhibit physiological dormancy and require stratification period to overcome seed dormancy. The highest germination percentage in the growing chamber subjected to GA3 process after eight weeks of stratification treatment was 62 % for Acer cappadocicum seeds. The highest germination percentage in greenhouse was obtained with cold stratification after eight weeks (95 %). It was found out that GA3 treatment had a significant effect on germination in growth chamber + 5 0C but GA3 treatment didn’t have a significant effect on germination in greenhouse conditions. GA3 treatment and soaking of unstratified seeds in water for 48 hr didn’t have any positive effect on germination value in greenhouse conditions. Although growth chamber and green house results both indicated that seed collection time did not seem to play a role as statistically on seed germination, Duncan’s test showed that the third seed collection time was in a different group.

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