scholarly journals La modelización en morfología y biología celular en el proceso de enseñanza-aprendizaje en las ciencias básicas en la formación médica / Modeling in morphology and cellular biology as a teaching-learning activity in basic sciences in the medical formation

2017 ◽  
Vol 7 (3) ◽  
pp. 8-13
Author(s):  
Luz Marina Cano Molano ◽  
Sandra Isabel Enciso Galindo ◽  
Jaime Andrés Gutiérrez Quintero ◽  
Martha Osorio de Sarmiento
Keyword(s):  
Cell Biology ◽  
Statistical Significance ◽  
Three Dimensional ◽  
Cellular Biology ◽  
Oral Exam ◽  
Learning Activity ◽  
Basic Sciences ◽  
Oral Test ◽  
Teaching Learning ◽  
The Impact

RESUMENIntroducción: Los procesos de enseñanza aprendizaje de las ciencias básicas tienen diferentes estrategias didácticas que permiten el desarrollo de las competencias de las asignaturas para la formación médica. Con el estudio teórico toda la tarea recae sobre la memoria y la imaginación, al contrario de lo que sucede si el trabajo se realiza de forma activa y las diferentes actividades realizadas requieren de todas las habilidades y sentidos. Objetivos: Evaluar el desempeño académico del estudiante cuando construye y sustenta un modelo tridimensional en las áreas de Biología Celular y Morfofisiología correlacionándolo con el examen oral. Métodos: Se realizó una encuesta de percepción estudiantil y se comparó con un t-student que permitió evaluar el impacto de la modelización en el rendimiento académico de los estudiantes. Resultados: La mediana del modelo fue de 3.38 y la mediana del examen oral fue de 3.75; existiendo una correlación del 84% entre la nota del modelo y el examen oral, con un coeficiente correlación de Pearson 0.85 con una p=0.000, estadísticamente significativa. Conclusión: La actividad académica de construcción de modelos tridimensionales en las áreas de Biología Celular y Morfofisiología para el desarrollo de la compresión de estructuras complejas mejora la capacidad de integración y argumentación de los contenidos de estas asignaturas.Palabras-Clave: Modelización; Ciencias básicas; Proceso enseñanza-aprendizajeABSTRACTIntroduction: The learning processes of the basic sciences have different teaching strategies that allow the development of the competences of the subjects for the training of practioners. With the theoreti-cal study, the whole task falls on memory and imagination, unlike what happens if the work is done in an active way and the different activities performed require all the skills and senses. Aims: To evaluate the student's academic performance when constructing and sustaining a three-dimensional model in the areas of Cell Biology and Morphology and correlate these with the oral exam. Methods: A student perception survey was conducted and compared with the one of a T-student that allowed the evaluation of the impact of the modelling on students' academic per-formance. Results: The average of the model was 3.38 and the average of the oral test was 3.75. There was a correlation of 84% between the model note and the oral test, with a correlation coefficient of Pear-son of 0.85 with a statistical significance p=0.000. Conclusion: The academic activi-ty of construction of three-dimensional models in the areas of Cellular Biology and Morphology for the development of the compression of complex structures im-proves the capacity of integration and argumentation of the contents of these subjects.Keywords: Modeling; Basic sciences; Teaching-learning process

scholarly journals Cell biology and histology in medicine: perception on education and student performance

2019 ◽  
Vol 3 (1) ◽  
pp. 8
Author(s):  
Ana Laura Sandoval Mantovani ◽  
Alba Regina De Abreu Lima ◽  
Sérgio Luís Aparecido Brienze ◽  
Emerson Roberto Dos Santos ◽  
Patrícia Da Silva Fucuta ◽  
...  
Keyword(s):  
Student Performance ◽  
Cell Biology ◽  
Selection Process ◽  
Cross Sectional Study ◽  
Cellular Biology ◽  
Pedagogical Practice ◽  
Cross Sectional ◽  
Final Grade ◽  
Glass Slides ◽  
Teaching Learning

The subjects areas of Cellular Biology and Histology, which underpin the microscopic field of Morphology and are included in the first years of the curriculum in a Medical course, are the pillars of medical education  and for the clinical reasoning of future professionals. This study aimed to trace a teaching profile of Cellular Biology and Histology in the Medical courses which had participants in the selection process to fill vacancies by transfer, for the undergraduate course in the Medical School of São José do Rio Preto (FAMERP)  in 2017. This was a quantitative and qualitative cross-sectional study and 132 academics participated. The results showed that, in most Higher Education Institutions, these disciplines are taught theoretically and practically. In the comparison between the theoretical performance of the students of traditional and active teaching-learning methodology, a higher number of hits in Histology and a better final grade were observed in the traditional. The deficiency pointed out by the students was a lack of laboratory sessions with viewing histological glass slides with light microscopes. Also, the aspect of teachers’ pedagogical practice and dynamic classes was shown to be fundamental to the students' interest, as well as to better satisfaction’s index in learning these areas. Furthermore, integrating these disciplines with the clinical practice of Medicine has proved to be relevant for academics, who feel more instigated to knowledge. In the present study, it was detected a better use in relation to the Histology by the students of the courses of traditional methodology, compared to those of active methodology and in this way the analysis done can to collaborate for the enrichment and reaffirmation of the fruits of the comparison of teaching-learning methodologies.

scholarly journals Environmental Flow Impacts on Tropical Cyclone Structure Diagnosed from Airborne Doppler Radar Composites

2013 ◽  
Vol 141 (9) ◽  
pp. 2949-2969 ◽  
Author(s):  
Paul D. Reasor ◽  
Robert Rogers ◽  
Sylvie Lorsolo
Keyword(s):  
Tropical Cyclone ◽  
Wind Shear ◽  
Doppler Radar ◽  
Statistical Significance ◽  
Three Dimensional ◽  
Vertical Wind Shear ◽  
Vertical Wind ◽  
Asymmetric Structure ◽  
The Impact ◽  
Airborne Doppler Radar

Abstract Following a recent demonstration of multicase compositing of axisymmetric tropical cyclone (TC) structure derived from airborne Doppler radar measurements, the authors extend the analysis to the asymmetric structure using an unprecedented database from 75 TC flights. In particular, they examine the precipitation and kinematic asymmetry forced by the TC's motion and interaction with vertical wind shear. For the first time they quantify the average magnitude and phase of the three-dimensional shear-relative kinematic asymmetry of observed TCs through a composite approach. The composite analysis confirms principal features of the shear-relative TC asymmetry documented in prior numerical and observational studies (e.g., downshear tilt, downshear-right convective initiation, and a downshear-left precipitation maximum). The statistical significance of the composite shear-relative structure is demonstrated through a stratification of cases by shear magnitude. The impact of storm motion on eyewall convective asymmetry appears to be secondary to the much greater constraint placed by vertical wind shear on the organization of convection, in agreement with prior studies using lightning and precipitation data.

scholarly journals Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1177
Author(s):  
Eve Hunter-Featherstone ◽  
Natalie Young ◽  
Kathryn Chamberlain ◽  
Pablo Cubillas ◽  
Ben Hulette ◽  
...  
Keyword(s):  
Cell Biology ◽  
Three Dimensional ◽  
Epidermal Keratinocytes ◽  
Mechanical Stimuli ◽  
Linc Complex ◽  
Mechanical Conditioning ◽  
Keratinocyte Cell ◽  
The Impact

Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and differentiation. In vitro cell culture, however, utilises tissue culture plastic, which is significantly stiffer than the in vivo environment. Current epidermal models fail to consider the effects of culturing keratinocytes on plastic prior to setting up three-dimensional cultures, so the impact of this non-physiological exposure on epidermal assembly is largely overlooked. In this study, primary keratinocytes cultured on plastic were compared with those grown on 4, 8, and 50 kPa stiff biomimetic hydrogels that have similar mechanical properties to skin. Our data show that keratinocytes cultured on biomimetic hydrogels exhibited major changes in cellular architecture, cell density, nuclear biomechanics, and mechanoprotein expression, such as specific Linker of Nucleoskeleton and Cytoskeleton (LINC) complex constituents. Mechanical conditioning of keratinocytes on 50 kPa biomimetic hydrogels improved the thickness and organisation of 3D epidermal models. In summary, the current study demonstrates that the effects of extracellular mechanics on keratinocyte cell biology are significant and therefore should be harnessed in skin research to ensure the successful production of physiologically relevant skin models.

scholarly journals Use of the movie “Lorenzo’s Oil” for didactic purposes in neuroscience and others health fields

2020 ◽  
Vol 14 (1) ◽  
pp. 7-13
Author(s):  
Lauana Lopes Gonçalves ◽  
Tales Alexandre Aversi-Ferreira
Keyword(s):  
Health Science ◽  
Learning Activity ◽  
Educational Approach ◽  
Active Teaching ◽  
Active Approach ◽  
Health Science Education ◽  
Initial Hypothesis ◽  
Teaching Learning ◽  
The Impact ◽  
Lorenzo’S Oil

ABSTRACT Although the traditional method of teaching is still the most popular nowadays, the use of different methodologies such as play approaches, for instance, could be used to make the teaching-learning process a more active approach. Nonetheless, the use of films that represent true stories are more pertinent in active teaching, especially those directly associated with a specific field and that are not merely dramatic. Lorenzo’s oil can inform students about many biological topics and problems linked to intensive care. Furthermore, it also addresses the impact of a neurological disease in a social environment and promotes an intrinsic discussion about sciences in general. Given the above, we propose the hypothesis that the film is useful for educational purposes in health, specifically neuroscience. Lorenzo’s Oil seems to be a good option for the use of a new approach in health science education. The richness of medical topics linked to modern aspects, such as nutrition for patients with mental disorders and palliative care combined with spirituality aspects, promotes an important discussion and constitutes a less stressing learning activity for students. Although some papers cite the importance of the movie for genetics and other fields, this paper shows the importance of efforts to address these topics using a more modern educational approach. According to the results presented, Lorenzo’s Oil could be used extensively for medical/health sciences, confirming the initial hypothesis.

The impact of confocal microscopy in biomedical research

1992 ◽  
Vol 50 (2) ◽  
pp. 1158-1159
Author(s):  
William B. Amos
Keyword(s):  
Cell Biology ◽  
Three Dimensional ◽  
Optical Microscope ◽  
Ion Concentration ◽  
Reaction Products ◽  
Embryonic Life ◽  
The Many ◽  
Calcium Ion Concentration ◽  
The Impact

The confocal optical microscope, using laser illumination, has now gained widespread acceptance (see volume edited by Pawley) Its advantage in providing clear optical sections, particularly with fluorescent specimens, is well known. Of the many confocal instruments now in use in cell biology, the applications can be classified into five different categories.The chief use is to give three-dimensional information about conventionally prepared fluorescent specimens. A notable example is the in vivo mapping of an identified neurone through several days of embryonic life by O'Rourke, Scott Fraser and colleagues at Irvine, USA. There has also been much work on in situ hybridisation, morphometry of solid tumours, oncogene product localisation and many aspects of the cytoskeleton.The second use has been in reflection imaging of cell surface contacts, of isolated microtubules and microorganisms, of parts of the eye and of reaction products such as peroxidase.The third application is the measurement of intracellular parameters such as pH and calcium ion concentration within a defined volume.

scholarly journals Three-dimensional model of glioblastoma by co-culturing tumor stem cells with human brain organoids

Biology Open ◽  
2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Roberta Azzarelli ◽  
Michela Ori ◽  
Anna Philpott ◽  
Benjamin D. Simons
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Fate ◽  
Cell Biology ◽  
Three Dimensional ◽  
3D Culture ◽  
Significant Degree ◽  
Culture Conditions ◽  
Three Dimensional Model ◽  
The Impact

ABSTRACT Emerging three-dimensional (3D) cultures of glioblastoma are becoming powerful models to study glioblastoma stem cell behavior and the impact of cell–cell and cell–microenvironment interactions on tumor growth and invasion. Here we describe a method for culturing human glioblastoma stem cells (GSCs) in 3D by co-culturing them with pluripotent stem cell-derived brain organoids. This requires multiple coordinated steps, including the generation of cerebral organoids, and the growth and fluorescence tagging of GSCs. We highlight how to recognize optimal organoid generation and how to efficiently mark GSCs, before describing optimized co-culture conditions. We show that GSCs can efficiently integrate into brain organoids and maintain a significant degree of cell fate heterogeneity, paving the way for the analysis of GSC fate behavior and lineage progression. These results establish the 3D culture system as a viable and versatile GBM model for investigating tumor cell biology and GSC heterogeneity. This article has an associated First Person interview with the first author of the paper.

Exploration of cell wall architecture with the rapid-freeze deep-etch technique

1993 ◽  
Vol 51 ◽  
pp. 128-129
Author(s):  
Béatrice Satiat-Jeunemaitre ◽  
Chris Hawes
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Cell Biology ◽  
Molecular Model ◽  
Three Dimensional ◽  
Secretory Activity ◽  
Wall Structure ◽  
Specimen Preparation ◽  
Molecular Architecture ◽  
Plant Cell Walls

The comprehension of the molecular architecture of plant cell walls is one of the best examples in cell biology which illustrates how developments in microscopy have extended the frontiers of a topic. Indeed from the first electron microscope observation of cell walls it has become apparent that our understanding of wall structure has advanced hand in hand with improvements in the technology of specimen preparation for electron microscopy. Cell walls are sub-cellular compartments outside the peripheral plasma membrane, the construction of which depends on a complex cellular biosynthetic and secretory activity (1). They are composed of interwoven polymers, synthesised independently, which together perform a number of varied functions. Biochemical studies have provided us with much data on the varied molecular composition of plant cell walls. However, the detailed intermolecular relationships and the three dimensional arrangement of the polymers in situ remains a mystery. The difficulty in establishing a general molecular model for plant cell walls is also complicated by the vast diversity in wall composition among plant species.

Percutaneous Coronary Intervention in Patients with Diabetes and Multivessel or Left Main Disease – a Review

2012 ◽  
Vol 7 (1) ◽  
pp. 37
Author(s):  
Donald E Cutlip ◽  
Keyword(s):  
Percutaneous Coronary Intervention ◽  
Coronary Artery ◽  
Statistical Significance ◽  
Bare Metal Stents ◽  
Coronary Intervention ◽  
Metal Stents ◽  
Increased Risk ◽  
Percutaneous Coronary ◽  
Patients With Diabetes ◽  
The Impact

Coronary artery disease in patients with diabetes is frequently a diffuse process with multivessel involvement and is associated with increased risk for myocardial infarction and death. The role of percutaneous coronary intervention (PCI) versus coronary artery bypass grafting (CABG) in patients with diabetes and multivessel disease who require revascularisation has been debated and remains uncertain. The debate has been continued mainly because of the question to what degree an increased risk for in-stent restenosis among patients with diabetes contributes to other late adverse outcomes. This article reviews outcomes from early trials of balloon angioplasty versus CABG through later trials of bare-metal stents versus CABG and more recent data with drug-eluting stents as the comparator. Although not all studies have been powered to show statistical significance, the results have been generally consistent with a mortality benefit for CABG versus PCI, despite differential risks for restenosis with the various PCI approaches. The review also considers the impact of mammary artery grafting of the left anterior descending artery and individual case selection on these results, and proposes an algorithm for selection of patients in whom PCI remains a reasonable strategy.

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