scholarly journals Application of Magnetic Resonance Image Intelligent Data Acquisition in Ovarian Cancer (Preprint)

2020 ◽  
Author(s):  
Tingting Liang ◽  
Yanlei Dong ◽  
Xinrui Zhao ◽  
Lu Wang ◽  
Hui Xu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Magnetic Resonance ◽  
Epithelial Cells ◽  
Data Acquisition ◽  
Cell Lines ◽  
P53 Protein ◽  
Control Group ◽  
Ovarian Epithelial Cells ◽  
Intelligent Data Acquisition ◽  
Experimental Group

UNSTRUCTURED As a diagnostic method with no radiation, high resolution of soft tissue, and different imaging methods, Magnetic Resonance Imaging (MRI) intelligent data acquisition is more and more widely used in the examination of abdominal, pelvic, and other organ lesions. In order to study the diagnostic effect of multi-mode magnetic resonance intelligent data acquisition on ovarian cancer and the ovarian cancer model modified based on p53-/-+Myc+ASAP1 gene, NSG mice were selected as experimental subjects in this study. 293FT cell lines packaging p53, Myc, and ASAP1(ArfGAP with SH3 domain, Ankyrin repeat and PH domain 1) recombinant lentivirus were inoculated into mouse ovarian epithelial cells to construct mouse ovarian epithelial cell tumor cell lines and their performance was analyzed. According to the different injection cell lines, they were divided into the experimental group and the control group. Tumor samples were collected and the mice were analyzed using immunofluorescence staining and MRI. The results showed that, in the detection of protein expression in genetically modified cell lines, for p53-/-+Myc+ASAP1 fully modified cell lines, the high expression of ASAP1 and Myc functional proteins was detected after the lentivirus containing p53-/-, ASAP1, and Myc were introduced into mouse ovarian epithelial cells, while the expression of p53 protein decreased significantly; after inoculation into mice, it was found that the expression of ASAP1 protein and Myc protein in the experimental group was significantly higher than that in the control group, while the expression of p53 protein in the experimental group was significantly lower than that in the control group, with significant statistical difference; further MRI diagnosis of two groups of mice showed that the ADC (Apparent dispersion coefficient) value of the experimental group was significantly higher than the control group, there were statistically significant differences. Therefore, it was found that p53 gene expression was down-regulated and Myc and ASAPl genes were overexpressed in the tumor tissues and tumor cells formed, and tumor formation differences between the two groups of mice could be obviously found after MRI intelligent data acquisition, which provided experimental basis for early diagnosis of breast cancer in the later clinical stage.

Adhesion of Malassezia pachydermatis of different growth type to canine epithelial cells

2014 ◽  
Vol 17 (2) ◽  
pp. 365-366 ◽  
Author(s):  
M. Florek ◽  
J. Król ◽  
Z. Staroniewicz ◽  
B. Bażanów
Keyword(s):  
Vitamin E ◽  
Epithelial Cells ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Control Group ◽  
Growth Type ◽  
Malassezia Pachydermatis ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

Mechanism of miRNA-26a on the Proliferation of Pancreatic Tumor Cells by Regulating the Expression of Cycline2

2020 ◽  
Author(s):  
Xiaochun HOU ◽  
Jian XU ◽  
Pei HU
Keyword(s):  
Epithelial Cells ◽  
Cell Lines ◽  
Pancreatic Tumor ◽  
Human Pancreatic Cancer ◽  
Control Group ◽  
Rt Pcr ◽  
Virus Particles ◽  
Apoptotic Protein ◽  
Proliferation Ability ◽  
Apoptosis Proteins

Background: qRT-PCR was used to measure the expression of miRNA-26a in pancreatic epithelial cells (HPDE) and human pancreatic cancer cell lines PANC-1 and MIA PaCa-2. Methods: PANC-1 and MIA PaCa-2 cell lines were infected with lentiviruses to construct PANC-miR-26a and MIA-miR-26a, and RT-PCR was used to detect the infection efficiency. The cell proliferation ability of PANC-miR-26a and MIA-miR-26a were examined by CCK-8 assay, and apoptosis was detected by flow cytometry. Western blotting was used to detect the expressions of CyclinE2 protein and mitochondria-associated apoptotic proteins. Results: miR-26a was expressed in human normal pancreatic epithelial cells (HPDE), and not detected in PANC-1 and MIA PaCa-2; miR-26a was highly expressed in the cell lines PANC-miR-26a and MIA-miR-26a infected by the virus particles. The absorbance values of PANC-miR-26a and MIA-miR-26a were lower than those of NC1 and PANC-1 in control group. The apoptosis rates of PANC-miR-26a and MIA-miR-26a were substantially higher than those of the control group. The overexpression of miR-26a inhibited the expression of the target protein CyclinE2 in PANC-miR-26a and MIA-miR-26a. The expression of the anti-apoptotic protein Bcl-2 was decreased in PANC-miR-26a and MIA-miR-26a, while the expression of the pro-apoptotic protein Bax was increased. Compared with HPDE, miR-26a was down-regulated in PANC-1 and MIA PaCa-2. After overexpression of miR-26a, the proliferation of PANC-1 and MIA PaCa-2 cell lines was weakened. Conclusion: Molecular mechanism is the negative regulation of CyclinE2 by miR-26a as well as the expressions of downstream mitochondrial apoptosis proteins Bcl-2 and Bax.

scholarly journals Support Vector Machine-Based Backprojection Algorithm for Detection of Gastric Cancer Lesions with Abdominal Endoscope Using Magnetic Resonance Imaging Images

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Huajia Dai ◽  
Yuhao Bian ◽  
Libin Wang ◽  
Junfeng Yang
Keyword(s):  
Magnetic Resonance Imaging ◽  
Gastric Cancer ◽  
Support Vector Machine ◽  
Magnetic Resonance ◽  
Hospital Stay ◽  
Radical Resection ◽  
Support Vector ◽  
Control Group ◽  
Resonance Imaging ◽  
Experimental Group

This study was to analyze the diagnostic value of magnetic resonance imaging (MRI) for gastric cancer (GC) lesions and the treatment effect of complete laparoscopic radical resection (CLSRR). A malignant tumor recognition algorithm was constructed in this study based on the backprojection (BP) and support vector machine (SVM), which was named BPS. 78 GC patients were divided into an experimental group (received CLSRR) and a control group (received assisted laparoscopic radical resection (ALSRR)), with 39 cases in each group. It was found that the BPS algorithm showed lower relative mean square error (MSE) in axle x (OMSE, x) and axle y (OMSE, x), but the classification accuracy (CA) was the opposite ( P < 0.05 ). The postoperative hospital stay, analgesia duration, first exhaust time (FET), and first off-bed activity time (FOBA) for patients in the experimental group were less ( P < 0.05 ). The operation time of the experimental group (270.56 ± 90.55 min) was significantly longer than that of the control group (228.07 ± 75.26 min) ( P < 0.05 ). There were 3 cases of anastomotic fistula, 1 case of acute peritonitis, and 2 cases of lung infections in the experimental group, which were greatly less than those in the control group (7 cases, 4 cases, and 3 cases) ( P < 0.05 ). In short, the BPS algorithm was superior in processing MRI images and could improve the diagnostic effect of MRI images. The CLSRR could reduce the length of hospital stay and the probability of complications in GC patients, so it could be used as a surgical plan for the clinical treatment of advanced GC.

scholarly journals Outcomes of extensive surgeries in combination treatment of stage III–IV ovarian cancer

2020 ◽  
Vol 10 (1) ◽  
pp. 43-49
Author(s):  
Kh. I. Mamazhonov ◽  
S. O. Nikogosyan ◽  
A. S. Shevchuk ◽  
V. V. Kuznetsov
Keyword(s):  
Ovarian Cancer ◽  
Lymph Node ◽  
Lymph Node Dissection ◽  
Intraoperative Complications ◽  
Stage Iii ◽  
Control Group ◽  
Node Dissection ◽  
Long Term Outcomes ◽  
Experimental Group

Objective: to evaluate short-term and long-term outcomes of lymph node dissection in patients with stage III–IV ovarian cancer.Materials and methods. This retrospective study included patients with stage III–IV ovarian cancer who have undergone either complete or optimal cytoreduction. Patients in the experimental group additionally had lymph node dissection, whereas patients in the control group had surgery without lymph node dissection. We evaluated 3‑year relapse-free survival (primary outcome measure), 3‑year overall survival, incidence of intraoperative and postoperative complications, and frequency of lymph node lesions.Results. The study included 272 patients: 43 women in the experimental group and 229 women in the control group. Intraoperative complications were significantly more common in patients who had lymph node dissection compared to those who had cytoreductive surgery alone (37.2 % vs 16.6 % respectively; р = 0.0001). The incidence of postoperative complications did not vary significantly between the groups (27.9 % in the experimental group vs 16.2 % in the control group; р = 0.128). Thirty-three patients (76.7 %) were found to have metastasis in the lymph nodes excised. The three-year overall survival rate was 82.6 % among patients who had lymph node dissection and 75.7 % among patients who had no lymph node dissection (р = 0.306). The three-year relapse-free survival rate was 26.2 % in the experimental group and 38.4 % in the control group (р = 0.858).Conclusions. Systemic lymph node dissection does not improve long-term outcomes and increases the incidence of intraoperative complications in patients with stage III–IV ovarian cancer undergoing complete or optimal cytoreduction.

scholarly journals Image Features of Magnetic Resonance Angiography under Deep Learning in Exploring the Effect of Comprehensive Rehabilitation Nursing on the Neurological Function Recovery of Patients with Acute Stroke

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Rui Yang ◽  
Ying Zhang ◽  
Miao Xu ◽  
Jing Ma
Keyword(s):  
Deep Learning ◽  
Magnetic Resonance ◽  
Magnetic Resonance Angiography ◽  
Acute Stroke ◽  
Image Features ◽  
Neurological Recovery ◽  
Control Group ◽  
Rehabilitation Care ◽  
Comprehensive Rehabilitation ◽  
Experimental Group

This study was to explore the effects of imaging characteristics of magnetic resonance angiography (MRA) based on deep learning on the comprehensive rehabilitation nursing on the neurological recovery of patients with acute stroke. In this study, 84 patients with acute stroke who were treated in hospital were selected as the research objects, and they were rolled into a control group (routine care) and an experimental group (comprehensive rehabilitation care). The dense dilated block-convolution neural network (DD-CNN) algorithm under deep learning for cerebrovascular was adopted to assess the effect of comprehensive rehabilitation care on the neurological recovery of patients with acute stroke. The results showed that the Berg scale scores, Fugl-Meyer scores, and Functional Independence Measure (FIM) scores of the experimental group of patients after 6 weeks and 12 weeks of comprehensive rehabilitation nursing were greatly different from those before treatment, showing statistical differences ( P < 0.05 ). Compared with conventional magnetic resonance imaging (MRI) images, MRA images based on CNN algorithm, Dense Net algorithm, and DD-CNN algorithm can more clearly show the patient’s cerebral artery occlusion. The average dice similarity coefficient (DSC) values of CNN algorithm, Dense Net algorithm, and DD-CNN algorithm were determined to be 84.3%, 95.7%, and 97.8%, respectively; the average sensitivity (Sen) values of the three algorithms were 76.1%, 95.4%, and 96.8%, respectively; and the average accuracy (Acc) values were 87.9%, 96.3%, and 97.9%, respectively. Thus, there were statistically obvious differences among the three algorithms in terms of average values of DSC, Sen, and Acc ( P < 0.05 ). The MRA images processed by the DD-CNN algorithm showed that the degree of neurological recovery of the experimental group was observably greater than that of the control group, and the difference was statistically obvious ( P < 0.05 ). In short, the image features of MRA based on the deep learning DD-CNN algorithm showed good application value in studying the effect of comprehensive rehabilitation nursing on the neurological recovery of patients with acute stroke, and it was worthy of promotion.

scholarly journals Berberine Sensitizes Human Ovarian Cancer Cells to Cisplatin Through miR-93/PTEN/Akt Signaling Pathway

2015 ◽  
Vol 36 (3) ◽  
pp. 956-965 ◽  
Author(s):  
Qiaoyun Chen ◽  
Rong Qin ◽  
Yue Fang ◽  
Hao Li
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Cell Lines ◽  
Ovarian Cancer Cell Line ◽  
Akt Signaling ◽  
Ovarian Cancer Cells ◽  
Control Group ◽  
Akt Signaling Pathway ◽  
Wide Range

Background: Berberine, a well-known component of the Chinese herbal medicine Huanglian, has wide range of biochemical and pharmacological effects, including antineoplastic effect, but the exact mechanisms remain unclear. The aim of the present study was to evaluate the potential chemo-sensitization effect of berberine in ovarian cancer cell line A2780. Methods: The expression of miR-93 was measure by RT-PCR. The target of miR-93 was confirmed by luciferase activity assay. Hoechst 33258 staining, Annexin V and PI double staining were used for apoptosis analysis. Results: In this study, we found A2780/DDP cells that were incubated with berberine combined with cisplatin had a significantly lower survival than the control group. Berberine enhanced cisplatin induced apoptosis and induced G0/G1 cell cycle arrest in A2780 cells. Next, we observed that the miR-93 levels in cisplatin resistant cell lines were higher than that in cisplatin sensitive cell lines. Furthermore, our study found berberine could inhibit miR-93 expression and function in ovarian cancer, as shown by an increase of its target PTEN, an important tumor suppressor in ovarian cancer. A2780 cells that were treated with PTEN siRNA had increased survival compared to NC group and this could be partly alleviated by the AKT inhibitor Triciribine. More importantly, A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression. Conclusion: The results suggested that berberine modulated the sensitivity of cisplatin through miR-93/PTEN/AKT signaling pathway in the ovarian cancer cells.

scholarly journals Efficacy of neoadjuvant hyperthermic intraperitoneal chemotherapy in advanced high-grade serous ovarian cancer (the NHIPEC trial): study protocol for a randomised controlled trial

BMJ Open ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. e046415
Author(s):  
Miao-Fang Wu ◽  
Li-Juan Wang ◽  
Yan-Fang Ye ◽  
Chang-Hao Liu ◽  
Huai-Wu Lu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Intraperitoneal Chemotherapy ◽  
Hyperthermic Intraperitoneal Chemotherapy ◽  
Controlled Trial ◽  
Progression Free Survival ◽  
Control Group ◽  
Chemotherapy Response ◽  
Open Label ◽  
Surgical Morbidity ◽  
Experimental Group

BackgroundNeoadjuvant chemotherapy (NACT) is an important treatment option for patients with ovarian cancer. Although intravenous NACT can improve optimal resection rates and decrease surgical morbidity and mortality, these advantages do not translate into a survival benefit. Ovarian carcinoma is mainly confined to the peritoneal cavity, which makes it a potential target for hyperthermic intraperitoneal chemotherapy (HIPEC). Our previous study showed that HIPEC could be used in the neoadjuvant setting, which was named neoadjuvant HIPEC (NHIPEC). Since hyperthermia is an excellent chemosensitiser, we hypothesised that the combination of NHIPEC and intravenous NACT could show superior efficacy to intravenous NACT alone.MethodsThis study is a single-centre, open-label, randomised (1:1 allocation ratio) phase 2 trial. A total of 80 patients will be randomly assigned into an experimental group (NHIPEC+intravenous NACT) or a control group (intravenous NACT). Patients in the experimental group will receive NHIPEC following laparoscopic evaluation, and four tubes will be placed via the laparoscopic ports, which will be used to administer NHIPEC. Then, perfusion with docetaxel (60–75 mg/m2) will be performed (43°C for 60 min, Day 0) followed by cisplatin (75 mg/m2, Day 1) infusion (43°C for 60 min) 24 hours later. After NHIPEC, two cycles of intravenous NACT will be given. Patients in the control group will receive three cycles of intravenous NACT. The primary endpoint is the proportion of patients who achieve a Chemotherapy Response Score (CRS) of 3 according to the CRS system. The secondary endpoints include progression-free survival, overall survival and the rates of complete resection and NHIPEC-related adverse events.Ethics approval and disseminationThis study was approved by the Ethics Committee of Sun Yat-sen Memorial Hospital (approval number: 2020-ky-050). Results will be submitted to peer-reviewed journals and presented at national and international conferences.Trial registration numberChiCTR2000038173.

scholarly journals Apoptosis of rats’ cardiomyocytes after chronic energy drinks consumption

2018 ◽  
Vol 31 (1) ◽  
pp. 25-28
Author(s):  
Miroslaw Aleksander Slawinski ◽  
Ewelina Wawryk-Gawda ◽  
Michal Konrad Zarobkiewicz ◽  
Pawel Halczuk ◽  
Barbara Jodlowska-Jedrych
Keyword(s):  
Cell Biology ◽  
Caspase 3 ◽  
P53 Protein ◽  
Energy Drinks ◽  
Control Group ◽  
Rat Cardiomyocytes ◽  
Male Wistar Rats ◽  
Staining Protocol ◽  
The Impact ◽  
Experimental Group

AbstractEnergy drinks (ED) are beverages containing caffeine, taurine, vitamins, herbal extracts, and sugar or sweeteners. They are marketed as capable of improving stamina, athletic performance and concentration, moreover, as serving as a source of energy. Still, there are very few papers describing the impact of ED on cell biology – including cell apoptosis within tissues. Therefore, in our study, we assessed the symptoms of rat cardiomyocytes apoptosis after 8 weeks consumption of ED.For the research, we used male Wistar rats divided into 2 groups (experimental and control). The experimental animals received ED at a dose average of 0.190 ml per g of body weight per day for a period of 8 weeks. The animals of the control group received just water and food without limitation. After 8 weeks, the rats were decapitated; hearts and other organs were collected. After embedding in paraffin blocks, 5μm thick tissue slides were prepared and stained according to standard hematoxylin and eosine (H&E) staining protocol. Additional slides were stained by immunohistochemistry with antibodies directed against either caspaze-3 or p53 protein.Our results showed that the expression of caspase 3 and p53 protein varied depending on the group of rats. The expression of caspase 3 observed in cardiomyocytes was much more intense in the experimental group compared to the control group. Furthermore, the immunoprecipitation of p53 protein was observed more frequently in the cardiomyocytes nuclei of the experimental group than in the control group.Obtained results suggest that chronic use of ED induces intracellular disorders and apoptosis in consumer cardiomyocytes.

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