scholarly journals Cloning and in silico study of an endoglucanase from a thermophilic bacterium isolated from a hydrothermal vent of West Kawio, Sangihe‐Talaud waters, North Sulawesi, Indonesia

2020 ◽  
Vol 24 (2) ◽  
pp. 105
Author(s):  
Edvan Arifsaputra Suherman ◽  
Maelita Ramdani Moeis ◽  
Elvi Restiawaty
Keyword(s):  
Amino Acids ◽  
High Temperatures ◽  
Hydrothermal Vent ◽  
Animal Feed ◽  
Thermophilic Bacteria ◽  
Glycosyl Hydrolase ◽  
Thermophilic Bacterium ◽  
Lower Percentage ◽  
Bacillus Species ◽  
North Sulawesi

Endoglucanase is used in industries that apply high temperatures, such as bioethanol, detergent, paper, and animal feed industries. Most available endoglucanases have very low stability at high temperatures. Therefore, this study aimed to identfy a new thermostable endoglucanase that is able to maintain its actvity at high temperatures. Five isolates of thermophilic bacteria were previously isolated from the hydrothermal vent of West Kawio, Indonesia. Among them, the DSI2 isolate showed the highest endoglucanase actvity, and was identfed and named as Bacillus safensis DSI2. The EgDSI2 gene was cloned from B. safensis DSI2. EgDSI2 is 1851 bp long encoding a protein of 616 amino acids. The encoded protein, EgDSI2, has high sequence identty to other B. safensis endoglucanases and was predicted with the Compute pI/Mw tool to be 69.41 kDa. EgDSI2 was high in hydrophobic amino acids. The enzyme had higher percentage of Ala andPro, and lower percentage of Gly compared to thermolabile endoglucanases from two Bacillus species. EgDSI2 harbored a catalytc domain belonging to glycosyl hydrolase family 9 (GH9) and a type 3 cellulose‐binding domain (CBM3). Propertes of endoglucanases with GH9‐CBM3 modular organizaton include actvity over a wide pH range, high optmum temperature, and thermostablity. Therefore, EgDSI2 has potental applicatons in the industries.

Taurine-related compounds and other free amino acids in deep-sea hydrothermal vent and non-vent invertebrates

2018 ◽  
Vol 165 (12) ◽  
Author(s):  
Tomoko Koito ◽  
Syuku Saitou ◽  
Toshihiro Nagasaki ◽  
Syosei Yamagami ◽  
Toshiro Yamanaka ◽  
...  
Keyword(s):  
Amino Acids ◽  
Deep Sea ◽  
Hydrothermal Vent ◽  
Free Amino Acids ◽  
Free Amino ◽  
Related Compounds

Lactoserum

2019 ◽  
pp. 432-456
Author(s):  
Charu Gupta ◽  
Consuelo Pacheco ◽  
Dhan Prakash
Keyword(s):  
Amino Acids ◽  
Nutritive Value ◽  
Animal Feed ◽  
Biological Activities ◽  
Tissue Growth ◽  
Growth Enhancement ◽  
Full Spectrum ◽  
Health Goals ◽  
Cheese Industry ◽  
Branched Chain

Lactoserum, a byproduct of cheese industry, is rich in nutrients, but it is discharged directly into the environment. It has many human applications that promise to be a complete nutraceutical for the future generations. It is of high nutritive value and its products can be used as functional ingredients in food and pharmaceutical applications and as nutrients in dietary and health foods. They contain full spectrum of amino acids including essential and branched-chain amino acids which are important in tissue growth and repair. The other biological activities of lactoserum are antibiotic, anti-cancer, anti-toxin, immune-enhancer, and prebiotic (growth enhancement of gut microflora such as bifidobacteria). Lactoserum can thus be used as nutraceutical in various products like infant formulas, food supplements, cheese spreads, sports bars, and beverages to meet a variety of health goals for people of all ages, including animal feed.

scholarly journals A Protein-Based Material from a New Approach Using Whole Defatted Larvae, and Its Interaction with Moisture

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 287 ◽  
Author(s):  
Nazanin Alipour ◽  
Björn Vinnerås ◽  
Fabrice Gouanvé ◽  
Eliane Espuche ◽  
Mikael S. Hedenqvist
Keyword(s):  
Amino Acids ◽  
Animal Feed ◽  
Saturated Fatty Acids ◽  
Moisture Sorption ◽  
Essential Amino Acids ◽  
Moisture Diffusivity ◽  
Size Analysis ◽  
Moisture Uptake ◽  
New Approach ◽  
Moisture Sorption Isotherm

A protein-based material created from a new approach using whole defatted larvae of the Black Soldier fly is presented. The larvae turn organic waste into their own biomass with high content of protein and lipids, which can be used as animal feed or for material production. After removing the larva lipid and adding a plasticizer, the ground material was compression molded into plates/films. The lipid, rich in saturated fatty acids, can be used in applications such as lubricants. The amino acids present in the greatest amounts were the essential amino acids aspartic acid/asparagine and glutamic acid/glutamine. Infrared spectroscopy revealed that the protein material had a high amount of strongly hydrogen-bonded β-sheets, indicative of a highly aggregated protein. To assess the moisture–protein material interactions, the moisture uptake was investigated. The moisture uptake followed a BET type III moisture sorption isotherm, which could be fitted to the Guggenheim, Anderson and de Boer (GAB) equation. GAB, in combination with cluster size analysis, revealed that the water clustered in the material already at a low moisture content and the cluster increased in size with increasing relative humidity. The clustering also led to a peak in moisture diffusivity at an intermediate moisture uptake.

scholarly journals Effects of Fermentation on Standardized Ileal Digestibility of Amino Acids and Apparent Metabolizable Energy in Rapeseed Meal Fed to Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1774
Author(s):  
Zhengke Wu ◽  
Jiao Liu ◽  
Jiang Chen ◽  
Shoaib Ahmed Pirzado ◽  
Yang Li ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Nutritive Value ◽  
Animal Feed ◽  
Basal Diet ◽  
Rapeseed Meal ◽  
Metabolizable Energy ◽  
Isoleucine Leucine ◽  
Ileal Digestibility ◽  
Apparent Metabolizable Energy

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.

scholarly journals Complete Genome Sequence of Thermoactinomyces vulgaris Strain CDF, a Thermophilic Bacterium Capable of Degrading Chicken Feathers

2019 ◽  
Vol 8 (28) ◽  
Author(s):  
Binbin Li ◽  
Feng Liu ◽  
Yuxia Ren ◽  
Yidi Ding ◽  
Yu Li ◽  
...  
Keyword(s):  
High Temperatures ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Thermophilic Bacterium ◽  
Content Type ◽  
Chicken Feathers ◽  
Thermoactinomyces Vulgaris

Thermoactinomyces vulgaris strain CDF was isolated from soil and shown to have the ability to degrade chicken feathers at high temperatures. Here, we report the complete genome sequence of this bacterium, which is 2,595,509 bp long with 2,642 predicted genes and an average G+C content of 48.14%.

GM technology: a tool to benefit livestock production

2004 ◽  
Vol 33 ◽  
pp. 247-258
Author(s):  
R.H. Phipps ◽  
A. Cockburn
Keyword(s):  
Amino Acids ◽  
Developing Countries ◽  
High Temperatures ◽  
Crop Residues ◽  
Livestock Production ◽  
Trypsin Inhibitors ◽  
Nutritional Factors ◽  
Maize Stover ◽  
Feed Resources ◽  
Gm Technology

The limitations to livestock production are numerous and varied but are applicable to a greater or lesser extent in both developed and developing countries. Crop factors that limit livestock production include inadequate quality (e.g. crop residues such as maize stover) and quantity of feed resources with an inconsistent supply due to extremes of climate (e.g. low rainfall and high temperatures), the presence of anti-nutritional factors and toxins (e.g. trypsin inhibitors, glucosinolates, gossypol, mycotoxins), and deficiencies of specific nutrients (e.g. amino acids and minerals).

scholarly journals Draft Genome Sequence of Caloranaerobacter sp. TR13, an Anaerobic Thermophilic Bacterium Isolated from a Deep-Sea Hydrothermal Vent

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Meixian Zhou ◽  
Yunbiao Xie ◽  
Binbin Dong ◽  
Qing Liu ◽  
Xiaoyao Chen
Keyword(s):  
Genome Sequence ◽  
Deep Sea ◽  
Hydrothermal Vent ◽  
East Pacific Rise ◽  
Draft Genome ◽  
Thermophilic Bacterium ◽  
Draft Genome Sequence ◽  
Biotechnological Application ◽  
East Pacific

Here, we report the draft 2,261,881-bp genome sequence of Caloranaerobacter sp. TR13, isolated from a deep-sea hydrothermal vent on the East Pacific Rise. The sequence will be helpful for understanding the genetic and metabolic features, as well as potential biotechnological application in the genus Caloranaerobacter .

Hydrolysis of proteins performed at high temperatures and for short times with reduced racemization, in order to determine the enantiomers of d- and l-amino acids

1997 ◽  
Vol 339 (1-2) ◽  
pp. 99-107 ◽  
Author(s):  
János Csapá ◽  
Zsuzsanna Csapó-Kiss ◽  
László Wágner ◽  
Tibor Tálos ◽  
Truman G. Martin ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Temperatures ◽  
Hydrolysis Of ◽  
Short Times

scholarly journals Morphological and Chemical Studies of the Spores and Parasporal Bodies of Bacillus laterosporus

1958 ◽  
Vol 4 (5) ◽  
pp. 639-650 ◽  
Author(s):  
Philip C. Fitz-James ◽  
I. Elizabeth Young
Keyword(s):  
Electron Microscopy ◽  
Amino Acids ◽  
Nucleic Acid ◽  
Total Nitrogen ◽  
Phosphorus Content ◽  
Bacillus Species ◽  
Chemical Studies ◽  
Bacillus Laterosporus ◽  
Total P ◽  
High Phosphorus Content

Spores of Bacillus laterosporus were studied to determine the chemical and morphological nature of their basophilic canoe-shaped parasporal bodies. An unusually high phosphorus content of these spores compared to other Bacillus species appeared to be associated with the parasporal body. Preparations of these "canoes" still attached to the spore coats were indeed high in phosphorus, but also in nitrogen. They were free of lipide-soluble and nucleic acid phosphorus and stained for protein. Some 50 per cent of the total nitrogen, but only 6 to 10 per cent of the total P were liberated by extraction with alkali-thioglycollate (pH 11.5) or alkali alone (pH 12.2–12.5). Proteinaceous material was recovered from these alkaline extracts and electron microscopy indicated that there had been a marked loss of "canoe" substance. Extraction with acid, removed some 80 per cent of the phosphorus associated with the "canoes" as orthophosphate. Chromatographic analyses for amino acids indicated some 14 ninhydrin-positive spots in the canoe-coat preparations whereas the whole spores contained at least 16.

Sign in / Sign up

Export Citation Format

Share Document