scholarly journals Susceptibility of Tiger Shrimp (Panaeus monodon) against Vibrio harveyi on various Molt Stage and Osmolarity

2016 ◽  
Vol 18 (1) ◽  
pp. 19 ◽  
Author(s):  
Gina Saptiani ◽  
Catur A Pebrianto ◽  
Agustina Agustina ◽  
Esti H Hardi
Keyword(s):  
Clinical Symptoms ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Pathological Anatomy ◽  
Molt Stage ◽  
East Kalimantan ◽  
Tiger Shrimp ◽  
Bacterial Content

Vibrio harveyi causes disease of tiger shrimp (Penaeus monodon) aquaculture in East Kalimantan. This research aimed to investigate the susceptibility of tiger shrimp on various molt stage and osmolarity, and determine the salinity (osmolarity) of water that can reduce the risk of V. harveyi attack. Shrimp age of 1.5 months was maintained in 4 aquariums with salinity 29.72 ‰, 26.07 ‰, 22.35 ‰ and 17.79 ‰, then challenged with V. harveyi. After 7 and 14 days, osmolarity of haemolimph was observed with automatic osmometer and Digital Roebling Osmometer. The susceptibility of tiger shrimp to V. harveyi was evaluated based on clinical symptoms, pathological anatomy of shrimp and density of V. harveyi (TPC) in the hepatopancreas. Osmolarities of shrimp haemolymph at premolt, postmolt, and intermolt stage were 575.30-812.60; 534.00-788.80, and 566.20-795.60 mOsm/l H2O, respectively. The lowest bacterial content in the premolt stage was maintained at a salinity 22.35 ‰, and the highest one was obtained in postmolt stage at 29.72 ‰. The molting and postmolt stage were the most susceptible to V. harveyi attacks. The results suggested that the shrimp maintained at 22.35 ‰ salinity was less susceptible to V. harveyi. 

scholarly journals ANALYSIS OF IMMUNE RESPONSES ON TRANSGENIC TIGER SHRIMP (Penaeus monodon) AGAINST PATHOGENIC BACTERIUM Vibrio harveyi

2014 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Andi Tenriulo
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Vibrio Harveyi ◽  
Gene Transfection ◽  
Penaeus Monodon ◽  
Pathogenic Bacterium ◽  
Shrimp Culture ◽  
Tiger Shrimp ◽  
Randomized Design ◽  
Study Results

Vibriosis is one of main diseases of the black tiger shrimp Penaeus monodon infected by pathogenic bioluminous bacterium Vibrio harveyi that can cause mass mortalities in shrimp culture. The bacteria can also trigger the disease white spot syndrome virus (WSSV). An effort to produce shrimp disease-resistant strains has been done through transgenesis technology with antiviral gene transfection. By this technology, it is expected an increase in the immune response of shrimp in a variety of diseasecausing pathogens. This study aimed to determine the immune responses (total haemocytes, haemocyte differentiation, and phenoloxydase activity) of transgenic tiger shrimp against pathogenic bacterium V. harveyi. Research using completely randomized design, which consists of two treatments and three replications. Test animals being used were transgenic and non-transgenic shrimp with size, weight 3.93±1.25 g and a total length of 7.59±0.87 cm. Treatments being tested were the injection of bacterium V. harveyi (density of 5x106 cfu/mL) of 0.1 mL/individual on transgenic (A) and non-transgenic shrimp (B). Immune response parameters such as total haemocytes, haemocyte differentiation, and phenoloxydase activity were observed on day 1, 3, and 6 days after challenging. Data were analyzed using t-test by SPSS software. The results showed that the total haemocyte of transgenic shrimp was not significantly different (P>0.05) from non-transgenic shrimp, but haemocyte differentiation and phenoloxydase activity were significantly different (P<0.05) especially on sixth days after being exposed to the bioluminescent bacteria. The study results implied that transgenic shrimp has a better immune response compared than non-transgenic shrimp.

scholarly journals The Effectiveness of Ascidian Didemnum molle Extracts as Antibactery Vibrio harveyi on Tiger Shrimp (Penaeus monodon)

2016 ◽  
Vol 15 (2) ◽  
Author(s):  
Christin Jelita Christin Jelita ◽  
Eri Bachtiar ◽  
Evi Liviawaty
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Tiger Shrimp

scholarly journals Response of hemocytes profile in the black tiger shrimp (Penaeus monodon) against Vibrio harveyi induced by Xylocarpus granatum leaves extract

2020 ◽  
Vol 13 (4) ◽  
pp. 751-757 ◽  
Author(s):  
Gina Saptiani ◽  
A. Syafei Sidik ◽  
Fikri Ardhani ◽  
Esti Handayani Hardi
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Water Extract ◽  
Negative Control ◽  
Distilled Water ◽  
Black Tiger Shrimp ◽  
Xylocarpus Granatum ◽  
Tiger Shrimp ◽  
Immersing Method

Aim: The present study investigated hemocytes profile of black tiger shrimp (Penaeus monodon) induced with Xylocarpus granatum leaves extract to protect against Vibrio harveyi infection. Materials and Methods: X. granatum leaves were chopped into small size, air-dried, and extracted with one of the following solvents: Ethanol, distilled water, and seawater, whereas each solvent was given in three different concentrations (750 ppm, 1.000 ppm, and 1.250 ppm, respectively). Extracts were induced to 60 post-larvae shrimp in each treatment (three replicates, 20 shrimp for each) by immersing method and subsequently challenged with V. harveyi. Results: This study demonstrated different effectiveness among solvents used to extract X. granatum leaves, in which distilled water showed the most effective solvent as can be seen from the lowest percentage on anorexia, lethargic, and weakened reflex of shrimp compared with another solvent, positive and negative controls. Pathological symptoms for shrimp induced by X. granatum leaves extract were minimum with the highest survival rate compared with those of positive and negative control. Total hemocyte cells and its cell constituents such as semi-granular, granular, and hyaline cells on treatment group at 1.250 ppm were higher than controls. Conclusion: Leaves extract of X. granatum extract effectively inhibited V. harveyi infection, increased survival rate, and hemocytes cell of the experimental shrimp. Distilled water extract of X. granatum at 1.250 ppm demonstrated the highest protective effect toward V. harveyi infection on P. monodon.

scholarly journals THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE

2014 ◽  
Vol 9 (2) ◽  
pp. 103
Author(s):  
Rosmiati Rosmiati ◽  
Najiah Musa ◽  
Habsah Mohammad ◽  
Tengku Sifzizul Tengku Muhammad ◽  
Andi Parenrengi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Marine Sponge ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Toxicity Study ◽  
Histological Observation ◽  
Black Tiger Shrimp ◽  
Butanol Extract ◽  
Tiger Shrimp ◽  
Bioactive Potential

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application.

scholarly journals Sonneratia alba Extract Protects the Post Larvae of Tiger Shrimp Penaeus monodon against Vibrio harveyi and Saprolegnia sp.

2020 ◽  
Vol 147 ◽  
pp. 01004
Author(s):  
Gina Saptiani ◽  
Andi Noor Asikin ◽  
Fikri Ardhani
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Saline Water ◽  
Penaeus Monodon ◽  
Clinical Signs ◽  
Effective Dosage ◽  
Relative Percentage ◽  
Tiger Shrimp ◽  
Percentage Survival ◽  
Sonneratia Alba

The research aimed to study Sonneratia alba leaves extract to inhibit Vibrio harveyi and Saprolegnia sp., and to evaluate its effectiveness to protect the post larvae of tiger shrimp against the infections. The leaves of S. alba were cleaned, chopped, dried, macerated and extracted in three different solvents namely ethanol 80%, freshwater and saline water. The post larvae 8 then immersed with the extract and infected with Vibrio harveyi and Saprolegnia sp. The clinical signs, pathological anatomy, total vibrio count, prevalence, survival rate, and relative percentage of survival were observed. The results showed that S. alba leaves extract inhibited V. harveyi and Saprolegnia sp. on post larvae of tiger shrimp, improve survival rate and relative percentage survival. The most effective dosage was 1,250 mg/L either for ethanolic, freshwater and saline water solvents.

Solanum nigrum enhancement of the immune response and disease resistance of tiger shrimp, Penaeus monodon against Vibrio harveyi

Aquaculture ◽  
2011 ◽  
Vol 318 (1-2) ◽  
pp. 67-73 ◽  
Author(s):  
Ramasamy Harikrishnan ◽  
Chellam Balasundaram ◽  
Sundaram Jawahar ◽  
Moon-Soo Heo
Keyword(s):  
Immune Response ◽  
Disease Resistance ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Solanum Nigrum ◽  
Tiger Shrimp

scholarly journals Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae

2015 ◽  
Vol 10 (2) ◽  
pp. 174
Author(s):  
Ade Dwi Sasanti ◽  
Widanarni . ◽  
Sukenda .
Keyword(s):  
Coral Reef ◽  
Survival Rate ◽  
16S Rrna ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Culture Method ◽  
In Vivo Test ◽  
Tiger Shrimp

<p>ABSTRACT</p><p><br />This study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.<br />Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reef</p><p><br />ABSTRAK</p><p><br />Penelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.<br /><br />Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang</p>

scholarly journals MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon) CULTURE

2015 ◽  
Vol 10 (1) ◽  
pp. 33
Author(s):  
Rosmiati Rosmiati ◽  
Andi Parenrengi ◽  
Emma Suryati
Keyword(s):  
Antibacterial Activity ◽  
Marine Sponge ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Shrimp Culture ◽  
Antibacterial Compounds ◽  
Potential Source ◽  
Tiger Shrimp

The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon) culture. The agar diffusion method using paper discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) and aqueous (HOE) and compounds successfully isolated against Vibrio harveyi. Findings showed that the antibacterial activity was concentrated in BUE with the inhibition zone of 17.2±0.1 mm. Meanwhile, two other extracts (DEE and HOE) did not exhibit any antibacterial activity against V. harveyi. From the active BUE, it was successfully isolated two compounds giving a strong anti-vibrio activity with the inhibition zone of 22±0.1 mm. The IR, 1H, 13C, COSY, HMQC, HMBC, and MS spectrum analysis indicated that both active compounds identified as aaptamine (1) and 9-demethyloxyaaptamine (2). The study suggested that marine sponge A. suberitoides may have potential compounds source for controlling of V. harveyi on tiger shrimp culture.

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