scholarly journals Peningkatan Jumlah Mikronukleus pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik

2013 ◽  
Vol 20 (2) ◽  
pp. 119 ◽  
Author(s):  
Rurie Ratna Shantiningsih ◽  
Suwaldi Suwaldi ◽  
Indwiani Astuti ◽  
Munakhir Mudjosemedi

Mikronukleus merupakan salah satu tanda awal terjadinya kerusakan DNA yang ditemukan pada mukosa gingiva manusia setelah paparan radiografi dental panoramik. Peningkatan jumlah mikronukleus terjadi paling tinggi pada hari ke-10 dan selanjutnya mengalami penurunan sampai dengan hari ke-14. Kelinci memiliki karakter dan periode turn-over mukosa gingiva yang hampir sama dengan manusia berkisar antara 10-12 hari. Tujuan penelitian ini untuk mengevaluasi apakah peningkatan jumlah mikronukleus pada mukosa gingiva kelinci setelah paparan radiografi panoramik. Sembilan ekor kelinci dibagi menjadi 3 kelompok untuk mewakili hari ke-3, 6 dan 9 setelah paparan radiografi panoramik. Sebelum dan sesudah diberikan paparan radiografi panoramik, setiap hewan coba dilakukan apusan pada mukosa gingiva anterior rahang bawah menggunakan cervical brush. Hasil apusan dilakukan pewarnaan dengan modifikasi Feulgen-Rossenbeck dan dihitung jumlah mikronukleus menggunakan mikroskop yang disambungkan dengan optilab. Analisis statistik dilakukan menggunakan paired t-test. Analisis statistik menunjukkan adanya perbedaan yang signifikan (p<0,05) antara jumlah mikronukleus sebelum dan 9 hari sesudah paparan radiografi panoramik. Akan tetapi tidak ditemukan perbedaan yang signifikan (p>0,05) antara sebelum paparan dibandingkan hari ke-3 dan ke-6 setelah paparan radiografi panoramik. Kesimpulang dari hasil penelitian ini sejalan dengan penelitian sebelumnya pada manusia bahwa peningkatan jumlah mikronukleus terjadi pada hari ke-9 setelah paparan radiografi panoramik. Hasil ini mengindikasikan bahwa pada kelinci juga menunjukkan peningkatan jumlah mikronukleus di mukosa gingiva akibat paparan radiografi panoramik.Micronucleus Increase After Panoramic Radiography Exposure In Rabbit’s Gingival Mucosa. Micronucleus is one of the early states of DNA damage found in human gingival mucosa after dental panoramic radiography exposure. The increasing amount of micronucleus will reach a peak in the tenth day after the exposure, and it will continuously decrease right after the fourteenth day. Rabbit has almost the same gingival mucosa and turn-over period with human for about 10-12 days. The purpose of this research is to evaluate the increasing amount of micronucleus in rabbit’s gingival mucosa after panoramic radiography exposure. A total of nine New Zealand rabbits were divided into 3 groups to represent day of 3rd, 6th  and 9th after the panoramic radiography exposure. The mandibular anterior gingival mucosa of each animals was swabbed using a cervical brush before and after panoramic radiography exposure. The samples were stained with Feulgen-Rossenbeck modification, and the amount of micronucleus was counted using a microscope that is connected to Optilab. Statistical analysis was performed using paired t-test. The statistical analysis showed that there was significant difference (p <0.05) between the number of micronucleus before exposure and 9th day after panoramic radiography exposure. Moreover, there was no significant difference (p> 0.05) between the amount of micronucleus before exposure compared with 3rd  and 6th  day after panoramic radiography exposure. Based on the experiment, it is concluded that the result is consistent with previous studies conducted in human that there was increasing amount of micronucleus at the 9th  day after panoramic radiography exposure. This result   indicates that rabbit   performs the increasing amount of micronucleus in gingival mucosa because of panoramic radiography exposure

2020 ◽  
Vol 32 (3) ◽  
pp. 233
Author(s):  
Dwiana Lisany Marunduri ◽  
Rosiliwati Wihardja ◽  
Winny Yohana

Introduction: Cranberry (Vaccinium macrocarpon) is commonly consumed as a source of antioxidants. The cranberry juice has a sour and a little bit of bitter taste that can affect the salivary pH. The sour taste is a result of its high content of proanthocyanidin. Cranberry juice plays a role in maintaining oral and dental health. Cranberries’ flavonol and proanthocyanidin content play a role in preventing dental caries by preventing the growth of Streptococcus mutans. This study was aimed to compare the salivary pH before and after cranberry juice consumption. Methods: This research was quasi-experimental. The research population was the students from the Faculty of Dentistry Universitas Padjadjaran, and the sampling method was purposive sampling. Thirty-five male and female students with no history of systemic disease and drug administration, no orthodontics appliance and denture, were involved in this experiment. Students with lesions on oral mucosa were also excluded from this study. The pH measurement was performed with a digital pH meter to record the salivary pH ratio before and after consumption of cranberry juice. Before salivary pH comparison analysis was performed, the normality test was conducted, then the analysis was continued using the paired t-test. Statistical analysis was determined at α=0.05. Results: The average values of salivary pH ratio before and after consumption of cranberry juice were 6.714 and 7.246, respectively. The normality test showed that the p-value before and after consumption of cranberry juice were 0.143 and 0.285, respectively (p-value>0.05), which categorised in the normal distribution. The paired t-test statistical analysis showed the p-value of 0.001 (p-value<0.05), which considered as significant. This result indicated that there was a significant difference in the salivary pH before and after consumption of cranberry juice. Conclusion: The salivary pH is increasing after cranberry juice consumption.


2020 ◽  
Vol 3 (1) ◽  
pp. 19-25
Author(s):  
Febria Restissa ◽  
Ahmad Rasyid ◽  
Devi Azri Wahyuni

ABSTRACT Objective : to determine the correlation between duration of ethambutol and copper levels in serum with color vision disorder examined with Farnsworth Munsell 100 Hue (FM100) in patients with tuberculosis. Methods: This study was an explorative observation of 20 samples during November 2017 - January 2018 period. Comparative analysis using independent t-test, paired t-test, Wilcoxon test and Mann-Whitney test, while correlation analysis using Spearman's test and Pearson's test.  Results: There was a significant difference of FM100 value before and after in category I (p=0.000) with increase of total error value 11.80+5.31 or category II (p=0.000) with increase of total error value 15.00+7.41. There was significant difference of Cu2+ level before and after in category I (p=0.005) with decrease of Cu2+ level 15.57+5.04 mg/dL or category II (p=0.005) with decrease of Cu2+ level 31.66+7.35 mg/dL. There was significant correlation between color vision and duration of ethambutol (p=0.000), but there was no significant correlation of Cu2+ with color vision (p>0.05). Conclusion: The duration of Ethambutol was related to changes in color vision examined with FM100.


2020 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Bismi Widi Hastari ◽  
Dyah Gandasari ◽  
Harry Harry

Tani Maju farmer group is one of the farming groups located in Rorotan Village, Cilincing District, North Jakarta City Administration. This group has a lot of sheep and advanced management. Despite they manage their sheep well, they are not well informed on how to manage the sewage from their castles (faces and urine). So far, they just throw away the sewage or gave them to fellow farmers. This study aimed to analyze the level of farmers' knowledge in making use of sheep's urine to be degraded into bio urine in the Maju Farmer group. Data was collected through the pre-test and post-test. The data were analyzed using paired t-test and Wilcoxon statistical software (IBM SPSS Statistics 25). The results of the study showed that farmers' knowledge in sewage (Urine) management increased; there is a significant difference in farmers' level of knowledge in bio urine production before and after bio urine extension.


2017 ◽  
Vol 36 (03) ◽  
Author(s):  
Kirti . ◽  
B. Jirli ◽  
Pankaj Kumar Mandal

Bihar is one of the poorest and most populous states in India. The share of employment in agriculture and allied activities is 68.9 percent in Bihar. This study is attempting to undertake a micro level analysis of collected data to assess the investment as well as return in Dairy activity carried out by Jeevika project in Muraul Block of Muzaffarpur District. Study revealed that calculated ‘t’ value of investment (12.533) and return (19.589) was more than table‘t’ value (1.977) of investment and return respectively. Comparison among investment as well as return in Dairy activity practiced by the respondents, before and after joining the project through paired t-test, showed a significant difference hence providing positive effect on the life of project beneficiaries.


2020 ◽  
Vol 5 (3) ◽  
pp. 101
Author(s):  
Anggun Dwi Andini ◽  
Rini Widyaningrum ◽  
Rurie Ratna Shantiningsih

Panoramic radiography is a radiography technique which is commonly used in dentistry. X-ray radiation from panoramic radiography can cause biological damage, such as the increase of gingival crevicular fluid (GCF) volume. One of materials which was known having the ability to decrease the effect of panoramic radiography is β-carotene in mucoadhesive gingival patch form. The application of β-carotene mucoadhesive gingival patch reduce micronucleus increasing in previous study. The aim of this study is to determine the effect of β-carotene mucoadhesive gingival patch application on palatal gingiva of maxillary incisor teeth to the GCF volume of panoramic radiography exposed patients. This study involved 10 subjects (5 females and 5 males). The GCF sample was taken from the labial sulcus of 11, 12 teeth (treatment group) and 21, 22 teeth (control group) of each subject before and after panoramic radiography exposure. β-carotene mucoadhesive gingival patch was applicated on palatal gingiva before panoramic radiography examination. Sample was collected using an absorbent paper which is inserted to the labial gingival sulcus. The GCF on the absorbent papers were marked by adding ninhydrin 2%, then the GCF volume was measured using a sliding caliper. Paired t-test result shows a significant difference between GCF volume in each group before and after panoramic radiography exposure (p<0.05). Independent t-test shows no significant difference between the GCF volume difference in the control and treatment group (p>0.05). The result of this study shows that the application of β-carotene mucoadhesive gingival patch on palatal gingiva of maxillary incisor teeth shows insignificant effect in decreasing the increase of GCF volume in panoramic radiography exposed patients.


2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Arnis Prilli Dharmastuti ◽  
Dwi Ariani Sulistyowati

Abstract: Hypoglycemia, Health Education, Knowledge, Patient. Hypoglycemia is one of the complications faced by people with diabetes mellitus, ie blood glucose levels fall below 50 to 60 mg / dl. Hypoglycemia may occur acutely, suddenly and can be life-threatening. Management of diabetes mellitus one of them is health education, especially about complications, namely Hypoglycemia. The objectives of this study were 1) to identify the level of knowledge of diabetes mellitus patients about prevention of hypoglycemia prior to health education 2) to identify the knowledge level of diabetes mellitus patients about hypoglycemia prevention after health education 3) to analyze knowledge change about prevention of hypoglycemia in patients with diabetes mellitus before and after the provision of health education in the intensive space of Dr Moewardi Hospital in 2016. This research type is experimental with pre-experimental design, variable approach using pre and post test designs method and data analysis used is Paired T-Test. The result of this research is there is influence of health education toward prevention effort of hypoglycemia in patient of diabetes mellitus in intensive room of Dr Moewardi Hospital 2016. This is proved by Paired T-Test test analysis on the value of knowledge before and after given health education obtained t count = -19,090, whereas t table value 2,042 so value -t arithmetic <-t table (-19,090 <-2,042) and sig. 0.000 (<0.05) then Ho is rejected and Ha accepted, meaning there is a significant difference between the value of knowledge before and after being given health education about hypoglycemia. There is an influence of health education on prevention of hypoglycemia in people with diabetes mellitus in intensive space Dr Moewardi Hospital in 2016.


2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Erna Ariyanti Kurnianingsih

Abstract: Sensory Integration Therapy-Muscle Tonus-Clinical Observation Of Neuromotor Performance. Special need children such as down syndrome, autism and intellectual disability suffered from Sensory Integration Disorder (SID) will have an impact on the weakness of postural muscle tone. Muscle tone measurement of children with SID is different from other children without SID. Clinical observation of neuromotor performance (CONP) is used as of one of criteria of muscle muscle tone in children with SID. The study aimed at finding theeffect of sensory integration therapy towards muscle tone based on Score of Clinical Observation of Neuromotor Performance (CONP) at Mitra Ananda Surakarta. The study was designed as quasi experiment one group pretest posttest design. population of this study is client with SID at Mitra Ananda Surakarta , by total sampling there are a number of 12 samples. Paired t-test is applied for analizing data with SPSS version 17.0. Paired t-test portrayed p-value > 0.05 it means that there are no significant difference in muscletone, before and after treatment. Sensory integration therapy doesn’t affect muscle tone for shildren with SID Based on Score of Clinical Observation of Neuromotor Performance (CONP) at Mitra Ananda Surakarta


Author(s):  
Ji Young Kim ◽  
Seong Yoel Kim

BACKGROUND: Hamstring flexibility plays a significant role in physiotherapy. Various stretching studies have been conducted to increase hamstring flexibility, but the effects of the Fascial Distortion Model (FDM) on hamstring flexibility have not yet been investigated. Moreover, no studies have compared the effects of FDM and static stretching. OBJECTIVES: To investigate the effects of the FDM on hamstring flexibility compared with static stretching. METHODS: Thirthy healthy adults were divided into two groups: static stretching and FDM groups. Static stretching was performed to hamstring in the supine position. The FDM was performed using trigger band techniques that followed the hamstring in a longitudinal direction. Hamstring flexibility was measured using the active knee extension angle (KEA), passive straight leg raising (SLR), sit-and-reach (SR), and finger-floor distance (FFD) tests. A paired t-test and an independent t-test were performed to compare the hamstring flexibility measurements. RESULTS: Both groups showed significant differences (p< 0.05) in KEA, SLR, SR, and FFD before and after. However, no significant difference (p> 0.05) was found between the two groups. CONCLUSIONS: There was no difference in the effects of FDM and static stretching on hamstring flexibility.


Author(s):  
Rindah Febriana Suryawati ◽  
Duhita Paramaramya Putri Nurdana

The problem faced by most micro-entrepreneurs in Indonesia is financing business. Peer-to-peer (P2P) lending is a non-bank financial institution that can be an alternative source of financing because of the requirements and easy application usage. This study aims to analyze the impact of peer-to-peer lending on business expenses, business turnover, total employment, total sales of products, and profits before and after obtaining a peer-to-peer lending loan and analyze factors affecting the increase in business turnover after getting a loan through peer-to-peer lending. The methods used in this study include the descriptive analysis method, paired t-test, and ordinary least square (OLS). The paired t-test results indicate that there is a significant difference between business expenses, business turnover, the amount of labor, the number of product sales, and profit before and after obtaining a peer-to-peer lending loan. The result of analysis with the OLS method shows that the length of business and expenditure of the business has a significant effect on the development of respondents' business turnover.


2019 ◽  
Vol 4 (1) ◽  
pp. 51
Author(s):  
Prasita Ayu Widyaningtyas

Abstract: Baby growth can be hampered if only given breast milk without getting breast milk supplementary food (MPASI) after the age of 6 months, because nutrients are not fulfilled for growth and development. However, if MPASI is given early it can cause negative problems in infants such as allergies and digestive disorders. One of the causes of malnutrition is due to the lack of knowledge of mothers about giving complementary feeding to infants. So that the mother provides complementary feeding at an earlier age and the type of food that is not suitable for the baby's age. This study aims to determine the differences in knowledge about complementary foods in mothers with underweight children aged 6-24 months before and after getting counseling in Mudung Village, Kepohbaru Bojonegoro Subdistrict. This study uses the pre-experimental method with the type of research using the one group pre test post test design. The sample in this study used a total sampling of 13 people. The data collection method used is a knowledge questionnaire. Data analysis was done by paired t test. Based on the results of the statistical test with paired t test obtained p value of (0.047) where the value of p value <0.05. Based on the results of the study it can be concluded that there is a significant difference between mother's knowledge about MP ASI to children aged 6-24 months before and after getting counseling. It is expected that mothers can apply complementary feeding behavior to infants aged 6-24 months correctly and accordingly in order to improve the nutritional status of infants and follow-up health workers to further improve health counseling to further improve health counseling about complementary foods.Keyword: counseling,complementary feeding, knowledgeAbstrak: Pertumbuhan bayi dapat terhambat apabila hanya diberi Air Susu Ibu (ASI) saja tanpa mendapat Makanan Pendamping Air Susu Ibu (MPASI) setelah berusia 6 bulan, karena tidak terpenuhinya zat gizi untuk pertumbuhan dan perkembangan. Namun, apabila MPASI diberikan secara dini dapat menimbulkan masalah negatif pada bayi seperti alergi dan gangguan pencernaan. Salah satu penyebab masalah gizi kurang karena tingkat pengetahuan ibu yang kurang tentang pemberian MPASI pada bayi. Sehingga ibu memberikan MPASI pada usia lebih dini dan jenis makanan MPASI yang tidak sesuai dengan usia bayi. Penelitian ini bertujuan untuk mengetahui perbedaan pengetahuan tentang makanan pendamping asi pada ibu dengan balita gizi kurang usia 6-24 bulan sebelum dan sesudah mendapatkan penyuluhan di Desa Mudung Kecamatan Kepohbaru Bojonegoro. Penelitian ini menggunakan metode pre-eksperimental dengan jenis penelitian menggunakan rancangan one grup pre test post test. Sampel dalam penelitian ini menggunakan total sampling sebanyak 13 orang. Metode pengumpulan data yang digunakan adalah kuesioner pengetahuan. Analisis data dilakukan dengan paired t test. Berdasarkan hasil dari uji statistik dengan paires t test diperoleh p value sebesar (0.047) dimana nilai p value < 0,05. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat perbedaan yang signifikan antara pengetahuan ibu tentang MP ASI kepada balita usia 6-24 bulan sebelum dan sesudah mendapatkan penyuluhan. Diharapkan ibu dapat menerapkan perilaku pemberian makanan pendamping ASI pada bayi umur 6-24 bulan secara benar dan sesuai agar meningkatkan status gizi bayi serta tindak lanjut petugas kesehatan untuk lebih meningkatkan penyuluhan kesehatan untuk lebih meningkatkan penyuluhan kesehatan tentang makanan pendamping ASI. Kata Kunci : Penyuluhan, MP ASI, Pengetahuan


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