scholarly journals β-Carotene patch application effects on gingival crevicular fluid volume after panoramic radiography exposure

2020 ◽  
Vol 5 (3) ◽  
pp. 101
Author(s):  
Anggun Dwi Andini ◽  
Rini Widyaningrum ◽  
Rurie Ratna Shantiningsih
Keyword(s):  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Panoramic Radiography ◽  
T Test ◽  
Maxillary Incisor ◽  
Patch Application ◽  
Significant Difference ◽  
Before And After ◽  
Crevicular Fluid ◽  
Β Carotene

Panoramic radiography is a radiography technique which is commonly used in dentistry. X-ray radiation from panoramic radiography can cause biological damage, such as the increase of gingival crevicular fluid (GCF) volume. One of materials which was known having the ability to decrease the effect of panoramic radiography is β-carotene in mucoadhesive gingival patch form. The application of β-carotene mucoadhesive gingival patch reduce micronucleus increasing in previous study. The aim of this study is to determine the effect of β-carotene mucoadhesive gingival patch application on palatal gingiva of maxillary incisor teeth to the GCF volume of panoramic radiography exposed patients. This study involved 10 subjects (5 females and 5 males). The GCF sample was taken from the labial sulcus of 11, 12 teeth (treatment group) and 21, 22 teeth (control group) of each subject before and after panoramic radiography exposure. β-carotene mucoadhesive gingival patch was applicated on palatal gingiva before panoramic radiography examination. Sample was collected using an absorbent paper which is inserted to the labial gingival sulcus. The GCF on the absorbent papers were marked by adding ninhydrin 2%, then the GCF volume was measured using a sliding caliper. Paired t-test result shows a significant difference between GCF volume in each group before and after panoramic radiography exposure (p<0.05). Independent t-test shows no significant difference between the GCF volume difference in the control and treatment group (p>0.05). The result of this study shows that the application of β-carotene mucoadhesive gingival patch on palatal gingiva of maxillary incisor teeth shows insignificant effect in decreasing the increase of GCF volume in panoramic radiography exposed patients.

scholarly journals Effect of β-carotene patch application on gingival crevicular fluid volume after repeated periapical radiographic exposure

2019 ◽  
Vol 4 (3) ◽  
pp. 127
Author(s):  
Faluthia Arini Puspitaningrum ◽  
Rurie Ratna Shantiningsih ◽  
Ryna Dwi Yanuaryska
Keyword(s):  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Biological Tissues ◽  
T Test ◽  
Paper Strip ◽  
X Rays ◽  
Patch Application ◽  
Before And After ◽  
Crevicular Fluid ◽  
Β Carotene

Free radicals generated during ionization process of X-rays can damage biological tissues. Radiation exposure to gingival sulcus area will damage endothelial cells and increase permeability of blood vessels under sulcular and junctional epithelium. That inflammation will increase gingival crevicular fluid (GCF) volume. Repeated periapical radiographs often occurs due to the unfulfillment of quality assurance and leads to an increase amount of radiation dose received by the patient. Previous studies have shown that β-carotene mucoadhesive gingival patch can penetrate mucous membrane and provide protection against radiation by reducing the number of gingival epithelial cells micronuclei. The aims of this study was to observe β-carotene mucoadhesive gingival patch effect in GCF volume from patient exposed to repeated periapical radiographs. We recruited 10 participants from patients who receive repeated periapical radiographs in instalation of dentomaxillofacial radiology, Prof Soedomo dental and oral hospital Faculty of Dentistry UGM. The teeth of the subjects are divided into control and treatment group. β-carotene mucoadhesive gingival patch was applied to treatment group. GCF was collected using an absorbing paper strip before and after exposure, then measured bysliding caliper. Paired T-test showed significant differences (p<0.05) between GCF volume before and after radiographic exposure in each group. Independent T-test showed significant differences (p<0.05) of GCF volume between control and treatment group. Conclusion of this study is β-carotene mucoadhesive gingival patch significantly reduce GCF volume after repeated periapical radiographic exposure.

scholarly journals Microbiota in Gingival Crevicular Fluid Before and After Mechanical Debridement With Antimicrobial Photodynamic Therapy in Peri-Implantitis

2022 ◽  
Vol 11 ◽  
Author(s):  
Haiyan Wang ◽  
Ye Liu ◽  
Wei Li ◽  
Wenyue Li ◽  
Hongtao Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Reference Database ◽  
Antimicrobial Photodynamic Therapy ◽  
Significant Difference ◽  
Before And After ◽  
Pre Treatment ◽  
Crevicular Fluid ◽  
Level 2

ObjectivesThis study aims to compare the microbiota of gingival crevicular fluid (GCF) before and after mechanical debridement (MD) with antimicrobial photodynamic therapy (aPDT) and determine the core efficient microbiota in peri-implantitis after treatment.MethodsWe recruited 9 patients (14 implants) treated with MD+aPDT for peri-implantitis at our center from February 1, 2018, to February 1, 2019. GCF was collected using filter paper strip before and after the treatment. The bacterial 16S rRNA was amplified and sequenced using an Illumina MiSeq platform to characterize the GCF. Bioinformatics and statistical analyses were performed using QIIME2 and R.ResultsA total of 4,110,861 high-quality sequences were obtained from GCF samples. Based on the reference database, 1,120 amplicon sequence variants (ASVs) were finally harvested. Principal coordinates analysis indicated significant differences in the bacterial community structure between the 180 days after-treatment group and pre-treatment group. Difference analysis and least discriminant analysis showed that the differences were mainly reflected in non-dominant bacteria between these two groups. The non-dominant genera with significantly different distribution between the 180 days after-treatment group and the pre-treatment group included Lactobacillus, Pedobacter, Bulleidia, Centipeda, Desulfovibrio, Ochrobactrum, Staphylococcus, Microbacterium, Brevundimonas, Desulfobulbus, and Parvimonas. Moreover, a total of 29 predictive functional categories at KEGG level 2 were identified. The significant difference pathways at KEGG level 2 between after-treatment and pre-treatment were concentrated in infectious disease-related pathways.ConclusionsPatients with peri-implantitis have significant changes in the low-abundance bacteria of the GCF before and after MD+aPDT. MD+aPDT may change the composition of GCF microbiota by increasing the abundance of cluster 1 (beneficial) and decreasing that of cluster 4 (harmful), which may decrease metabolic response to infection and thus improve peri-implantitis.

scholarly journals THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS

2021 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Rini Rahmiyati ◽  
Didit Aspriyanto ◽  
Beta Widya Oktiani
Keyword(s):  
Filter Paper ◽  
Wistar Rats ◽  
Gingival Crevicular Fluid ◽  
Panoramic Radiography ◽  
Cell Damage ◽  
Biological Fluid ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Crevicular Fluid

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

scholarly journals Pengaruh Teknik Relaksasi Afirmasi terhadap Stres Mahasiswa yang Menempuh Skripsi di Program Studi Ilmu Keperawatan Universitas Jember

2018 ◽  
Vol 6 (2) ◽  
pp. 319
Author(s):  
Rommiyatun Zainiyah ◽  
Erti Ikhtiarini Dewi ◽  
Wantiyah Wantiyah
Keyword(s):  
Treatment Group ◽  
Sampling Technique ◽  
T Test ◽  
Control Group ◽  
Final Project ◽  
Parasympathetic Nerves ◽  
Significant Difference ◽  
Before And After ◽  
Post Test ◽  
And Control

One of stressor in college students is taking a final project as a graduation requirement. The body'sresponse toward stress includes a rise of heart rate, diarrhea and insomnia. One of stressmanagement is affirmation relaxation (AR) technique. This research aimed to analyze the influenceof AR technique towards stress on students who take a final project in Faculty of Nursing, UniversityOf Jember. This research used a quasi experiment with Pretest-Post test approach and controlgroup design. Sampling technique that used is purposive sampling with respondent divided into twogroups, the treatment group and control group with 15 respondents for each group. Data wasanalyzed by using Dependent t test and Independent t test with 95% CI. The result showed thatthere was a significant difference stress before and after given AR technique within average 19,2 to10,87 (p = 0,001). Mean while, there was no significant difference of stress between pretest and posttest in control group within average 18,73 to 18,4 (p = 0,841). Overall, there was a difference ofstress between treatment group and control group (p =0,02, α = 0,05). The AR techniques reducestress because it can influence the sympathetic and parasympathetic nerves to produce endorphinehormone. The conclusion of this research is there was an influence of AR technique towards stresson students who Taking a Final Project in Faculty of Nursing University of Jember.Keywords: affirmation relaxation technique, stress, final project.

scholarly journals Efek Aplikasi Patch Gingiva Mukoadesif β-Carotene Akibat Paparan Radiografi Panoramik

2015 ◽  
Vol 20 (2) ◽  
pp. 186 ◽  
Author(s):  
Rurie Ratna Shantiningsih ◽  
Silviana Farrah Diba
Keyword(s):  
New Zealand ◽  
Panoramic Radiography ◽  
Scale Analysis ◽  
Gray Scale ◽  
Protection Effect ◽  
Patch Application ◽  
Group I ◽  
Significant Difference ◽  
First Time ◽  
Β Carotene

Pada penelitian sebelumnya diketahui bahwa aplikasi patch gingiva mukoadesif β-carotene berfungsi sebagai bahan proteksi efek paparan radiografi panoramik pada hewan coba kelinci New Zealand. Peningkatan jumlah mikronukleus akibat paparan radiografi panoramik dapat dihambat dengan aplikasi patch β-carotene. Sebagai kelanjutan dari penelitian, dilakukan uji klinis terhadap manusia untuk melihat efek proteksi patch tersebut. Sebanyak 20 orang subjek yang akan dipapar radiografi panoramik dimasukkan secara random dalam 2 kelompok. Kelompok I adalah subjek tanpa aplikasi patch gingiva mukoadesif β-carotene dan Kelompok II adalah subjek yang diaplikasi patch sebelum paparan. Setiap subjek dilakukan apusan pada mukosa gingiva anterior rahang atas sebelum dan 10 hari setelah paparan radiografi panoramik. Apusan diwarnai dengan metode modifikasi Feulgen-Rossenbeck. Efek lain dari aplikasi juga dilihat mengenai lama waktu bertahan patch dan hasil radiograf yang dihasilkan. Hasil penelitian menunjukkan bahwa terdapat penurunan jumlah mikronukleus pada kelompok II walaupun tidak berbeda bermakna (p>0,05) terhadap kelompok I. Lama waktu bertahan patch gingiva mukoadesif β-carotene dapat mencapai lebih dari 10 jam dan dilaporkan terasa mengganjal pada awal pemakaian. Hasil analisis gray scale antara radiograf dengan dan tanpa aplikasi tidak menunjukkan perbedaan yang bermakna (p>0,05). Kesimpulan dari penelitian ini adalah aplikasi patch gingiva mukoadesif β-carotene dapat menurunkan jumlah mikronukleus namun tidak secara signifikan.Effect of β-carotene Gingival Mucoadhesive Patch Application at Panoramic Radiography Exposure. According to previous research, β-carotene gingival mucoadhesive patch has a radiation protection effect on New Zealand Rabbits. The increase in micronucleus after panoramic radiography exposure is avoided because of β-carotene gingival mucoadhesive patch application. In order to continue that research, we try the application of β-carotene gingival mucoadhesive patch into human. Twenty subjects who requested to take panoramic radiography were divided into two groups randomly. Group I was without β-carotene gingival mucoadhesive patch application and Group II was given the application before radiography exposure. Each of the subjects was swabbed on anterior maxillary gingiva before radiography exposure and the 10th day after the exposure. The swab was stained using modified Feulgen Rossenbeck method. The adhesive time and the effect of radiograph were also observed. The result shows that there was a decrease in micronucleus number after β-carotene gingival mucoadhesive patch application but there was no significant difference (p>0,05) between Grup I and II. The adhesive time was more than 10 hours and there was uncomfortable taste in the first time application. Gray scale analysis shows no significant difference (P>0,05) between panoramic radiography application and without application. The conclusion is that β-carotene gingival mucoadhesive patch application reduces micronucleus number although not significantly.

scholarly journals PENDIDIKAN GIZI MENGGUNAKAN MEDIA BUKU SAKU MENINGKATKAN PENGETAHUAN DAN PRAKTIK KONSUMSI SAYUR DAN BUAH PADA SISWA SEKOLAH DASAR

2018 ◽  
Vol 12 (2) ◽  
pp. 107
Author(s):  
Fitria Laras Azadirachta ◽  
Sri Sumarmi
Keyword(s):  
Treatment Group ◽  
Knowledge Score ◽  
T Test ◽  
Rank Test ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Before And After ◽  
Post Test ◽  
Vegetables And Fruits

The average consumption of vegetables and fruits in Indonesia is still low. This condition can cause the emergence of nutritional problems in children. The purpose of this study was to determine the effect of nutritional education using pocketbook to increasing knowledge and practice of vegetables and fruits consumption among elementary students. This research was quasy experiment using pre-test post-test control group design. The sample size was 60 students consisting of 20 students of SDN Kandang Tepus I as control group treated with media leaflet and 40 students of SDN Kandang Tepus II as treatment group given the pocketbook. The result of independent t-test in pre-test of booth groups shows that there was no difference in knowledge (p=0.220) and practice (p=0.091) between the two groups. Independent t-test result on post-test also shows was no difference in knowledge (p=0.421) and practice (p=0.810) between the two groups. The result of paired sample t-test of the treatment group showed the average of pre-test and post-test of knowledge score were 31.50 and 66.25 and the practice were 2.58 and 3.08. There were significant difference of knowledge (p=0.000) and practice (p=0.000) before and after being given pocketbook. Wilcoxon sign rank test in the control group showed that leaflet did not affect knowledge (p=0.052) but affect the practice (p=0.001). It can be concluded that use of pocketbook media influence the knowledge and the respondent practice.

scholarly journals Biological changes after dental panoramic exposure: conventional versus digital

2018 ◽  
Vol 51 (1) ◽  
pp. 25
Author(s):  
Rurie Ratna Shantiningsih ◽  
Silviana Farrah Diba
Keyword(s):  
Gingival Crevicular Fluid ◽  
Biological Effects ◽  
Panoramic Radiograph ◽  
Conventional Radiography ◽  
Radiographic Technique ◽  
Significant Difference ◽  
Before And After ◽  
The Difference ◽  
Crevicular Fluid ◽  
Dental Digital Radiography

Background: Dental digital radiography is more practical and requires fewer doses of radiation than conventional radiography. Because ionizing radiation has a biological effect on exposed tissue, concerns regarding its stochastic effect merit greater attention. In a previous study, it was found that biological changes and increases in the micronucleus occurred after conventional panoramic exposure to gingival crevicular fluid (GCF). Purpose: The purpose of this study is to investigate the difference in biological effects after digital panoramic exposure compared with conventional exposure. Methods: Twenty subjects were classified into two groups according to the radiographic technique employed. The techniques consisted of ten subjects undergoing digital panoramic radiograph exposure and ten others being subjected to conventional exposure. GCF calculated in mm3 was collected by applying paper strips to the anterior maxillary labial gingival sulcus for one minute prior to and ten minutes after exposure. A micronucleus was obtained from a gingival smear on the same quadrant ten days after panoramic exposure. Results: There was a significant difference in the number of micronuclei between conventional and digital panoramic radiographs both before and after exposure (p=0.000). In contrast, increased GCF volume was not statistically significant (p=0.506) before or after digital panoramic exposure, while the significant difference of conventional panoramic exposure was p=0.017. Conclusion: Digital panoramic radiograph exposure induced a biological change only in terms of an increase in the number of micronuclei but not in the volume of GCF.

scholarly journals Peningkatan Jumlah Mikronukleus pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik

2013 ◽  
Vol 20 (2) ◽  
pp. 119 ◽  
Author(s):  
Rurie Ratna Shantiningsih ◽  
Suwaldi Suwaldi ◽  
Indwiani Astuti ◽  
Munakhir Mudjosemedi
Keyword(s):  
Statistical Analysis ◽  
Panoramic Radiography ◽  
T Test ◽  
Early States ◽  
Significant Difference ◽  
Before And After ◽  
Gingival Mucosa ◽  
Turn Over ◽  
Paired T Test ◽  
New Zealand Rabbits

Mikronukleus merupakan salah satu tanda awal terjadinya kerusakan DNA yang ditemukan pada mukosa gingiva manusia setelah paparan radiografi dental panoramik. Peningkatan jumlah mikronukleus terjadi paling tinggi pada hari ke-10 dan selanjutnya mengalami penurunan sampai dengan hari ke-14. Kelinci memiliki karakter dan periode turn-over mukosa gingiva yang hampir sama dengan manusia berkisar antara 10-12 hari. Tujuan penelitian ini untuk mengevaluasi apakah peningkatan jumlah mikronukleus pada mukosa gingiva kelinci setelah paparan radiografi panoramik. Sembilan ekor kelinci dibagi menjadi 3 kelompok untuk mewakili hari ke-3, 6 dan 9 setelah paparan radiografi panoramik. Sebelum dan sesudah diberikan paparan radiografi panoramik, setiap hewan coba dilakukan apusan pada mukosa gingiva anterior rahang bawah menggunakan cervical brush. Hasil apusan dilakukan pewarnaan dengan modifikasi Feulgen-Rossenbeck dan dihitung jumlah mikronukleus menggunakan mikroskop yang disambungkan dengan optilab. Analisis statistik dilakukan menggunakan paired t-test. Analisis statistik menunjukkan adanya perbedaan yang signifikan (p<0,05) antara jumlah mikronukleus sebelum dan 9 hari sesudah paparan radiografi panoramik. Akan tetapi tidak ditemukan perbedaan yang signifikan (p>0,05) antara sebelum paparan dibandingkan hari ke-3 dan ke-6 setelah paparan radiografi panoramik. Kesimpulang dari hasil penelitian ini sejalan dengan penelitian sebelumnya pada manusia bahwa peningkatan jumlah mikronukleus terjadi pada hari ke-9 setelah paparan radiografi panoramik. Hasil ini mengindikasikan bahwa pada kelinci juga menunjukkan peningkatan jumlah mikronukleus di mukosa gingiva akibat paparan radiografi panoramik.Micronucleus Increase After Panoramic Radiography Exposure In Rabbit’s Gingival Mucosa. Micronucleus is one of the early states of DNA damage found in human gingival mucosa after dental panoramic radiography exposure. The increasing amount of micronucleus will reach a peak in the tenth day after the exposure, and it will continuously decrease right after the fourteenth day. Rabbit has almost the same gingival mucosa and turn-over period with human for about 10-12 days. The purpose of this research is to evaluate the increasing amount of micronucleus in rabbit’s gingival mucosa after panoramic radiography exposure. A total of nine New Zealand rabbits were divided into 3 groups to represent day of 3rd, 6th  and 9th after the panoramic radiography exposure. The mandibular anterior gingival mucosa of each animals was swabbed using a cervical brush before and after panoramic radiography exposure. The samples were stained with Feulgen-Rossenbeck modification, and the amount of micronucleus was counted using a microscope that is connected to Optilab. Statistical analysis was performed using paired t-test. The statistical analysis showed that there was significant difference (p <0.05) between the number of micronucleus before exposure and 9th day after panoramic radiography exposure. Moreover, there was no significant difference (p> 0.05) between the amount of micronucleus before exposure compared with 3rd  and 6th  day after panoramic radiography exposure. Based on the experiment, it is concluded that the result is consistent with previous studies conducted in human that there was increasing amount of micronucleus at the 9th  day after panoramic radiography exposure. This result   indicates that rabbit   performs the increasing amount of micronucleus in gingival mucosa because of panoramic radiography exposure

scholarly journals Comparison of Copper Level and Color Vision in Pulmonary Tuberculosis Patients Before and After Ethambutol Treatment in Category I and II

2020 ◽  
Vol 3 (1) ◽  
pp. 19-25
Author(s):  
Febria Restissa ◽  
Ahmad Rasyid ◽  
Devi Azri Wahyuni
Keyword(s):  
Color Vision ◽  
Total Error ◽  
T Test ◽  
Wilcoxon Test ◽  
Copper Level ◽  
Tuberculosis Patients ◽  
Vision Disorder ◽  
Significant Difference ◽  
Before And After ◽  
Paired T Test

ABSTRACT Objective : to determine the correlation between duration of ethambutol and copper levels in serum with color vision disorder examined with Farnsworth Munsell 100 Hue (FM100) in patients with tuberculosis. Methods: This study was an explorative observation of 20 samples during November 2017 - January 2018 period. Comparative analysis using independent t-test, paired t-test, Wilcoxon test and Mann-Whitney test, while correlation analysis using Spearman's test and Pearson's test.  Results: There was a significant difference of FM100 value before and after in category I (p=0.000) with increase of total error value 11.80+5.31 or category II (p=0.000) with increase of total error value 15.00+7.41. There was significant difference of Cu2+ level before and after in category I (p=0.005) with decrease of Cu2+ level 15.57+5.04 mg/dL or category II (p=0.005) with decrease of Cu2+ level 31.66+7.35 mg/dL. There was significant correlation between color vision and duration of ethambutol (p=0.000), but there was no significant correlation of Cu2+ with color vision (p>0.05). Conclusion: The duration of Ethambutol was related to changes in color vision examined with FM100.

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