scholarly journals ETHANOLIC EXTRACTS OF HEDYOTIS CORYMBOSA L. IMPROVES MONOSODIUM IODOACETATE-INDUCE OSTEOARTHRITIS IN RAT

2017 ◽  
Vol 10 (3) ◽  
pp. 473
Author(s):  
Anton Bahtiar ◽  
Fitri Arum Sari ◽  
Mega Audina Putri ◽  
Natasha Linsie Corona Datunsolang ◽  
Ade Arsianti
Keyword(s):  
Immune System ◽  
Ethanolic Extract ◽  
Daily Practice ◽  
Negative Control Group ◽  
Negative Control ◽  
Male Rats ◽  
Knee Joints ◽  
Control Group ◽  
Sprague Dawley ◽  
Monosodium Iodoacetate

ABSTRACTObjective: The objective of this study is to explore the effects of the 70% ethanolic extract of pearl grass on the immune system of the osteoarthritismodel rat, characterized by the number of leukocytes and lymphocytes, and the histology of the joint. Osteoarthritis is a degenerative diseasecharacterized by chronic inflammation in the joints. Based on the daily practice of herbal medicine in some community in Indonesia, pearl grassusually used for anti-inflammation but not a lot of data to support it.Methods: We used 36 male rats Sprague-Dawley strain divided into 6 groups. Normal group was given 0.5% of CMC, the negative control group was given0.025 ml of sodium iodoacetate in 0.9% saline, the positive groups control group was given a suspension of glucosamine-chondroitin 135 mg/200 g bb,three were given pearls grass extract in various dose 5.625 mg, 11.25 mg, and 22.5 mg, respectively. 28 days after sodium iodoacetate induction, the extractswere given orally once daily for 21 days. Measurement of inflammation of knee joint and the number of leukocytes and lymphocytes were counted on day14th, 28th, and 49th after sodium iodoacetate induction. After treatment, all rats were sacrified and all knee joints were collected to subject for histology.Results: The results showed that the extract of pearl grass in all doses was able to decrease the number of leukocytes and lymphocytes significantlyand prevent proteoglycan degradation. The results showed that the extract of pearl grass with a given dose variations have antiinflammation effectand been able to protect proteoglycan significantly.Conclusion: Doses 3 (22.5 mg/200 g BW) is the best result. These results indicate that pearl grass can be further investigated as a treatment for osteoarthritis.Keyword: Osteoarthritis, Sodium iodoacetate, Pearl grass, Hedyotis corymbosa L. Lamk., Immune system. 

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

scholarly journals The Increase Activity of Lipoprotein Lipase (LPL) Enzyme and Histophatological Changes of Liver of Hypercholesterolemic Rat (Rattus norvegicus) Induced by Ethanolic Extract of Ant Plant (Myrmecodia sp.)

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Roslizawaty R ◽  
Rusli R ◽  
Nazaruddin N ◽  
Syafruddin S ◽  
Indahlia Syahfitri Bangun ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Lipoprotein Lipase ◽  
Liver Damage ◽  
Fatty Infiltration ◽  
Ethanolic Extract ◽  
Fatty Degeneration ◽  
Negative Control Group ◽  
Negative Control ◽  
Male Rats ◽  
Control Group

This study was aimed to find out the effect of ethanolic extract of ant plant (Myrmecodia sp.) to increase the activity of enzyme lipoprotein lipase (LPL) serum and to observe the histopathological changes of hypercholesterolemic rat liver. This study used 20 male rats grouped into 4treatment groups, namely negative control group (K1), hypercholesterolemic group (K2), and hypercholesterolemic group that administered withethanolic extract of ant plant 100 (K3) and 200 mg/kg bw (K4). The LPL enzyme activity were measured by the titration method andhistopatological changes of liver were observed by calculated fatty degeneration and fatty infiltration. The data were analyzed using one wayanova followed by Duncan test. The average of LPL enzyme activity on group K1, K2, K3, and K4 were 0.80±0.06, 0.45±0.10, 0.83±0.11, and0.76±0.03 unit, respectively. The average number of fatty degeneration on hepatocyte and fatty infiltration were 1.80±0.83, 3.60±1.14, 23.00±1.22, and 40.20±1.30; and 9.20±0.84, 16.40±1.14, 2.60±0.54, and 4.80±0.83, respectively. The results showed that theraphy ethanolic extract ofant plant effects significantly (P<0.01) on the increase of enzyme LPL and improve liver damage in hypercholesterolemic male rats. To concludethe administration of ethanolic extract of ant plant increases the LPL enzyme activity and improves liver damage on hyperch olesterolemic rats.Key words: Myrmecodia sp., LPL activity, histopathological liver, hypercholesterolemia

scholarly journals The Effect of Ethanolic Extract of Roselle (Hibiscuss sabdariffa L) on Increasing SOD Activity

2016 ◽  
Vol 5 (1) ◽  
pp. 118
Author(s):  
Abdul Rahman W ◽  
Nurkhasanah Nurkhasanah ◽  
Nanik Sulistyani
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Activity Data ◽  
Group Iii

Free radicals were reactive species caused oxidation of lipids membrane and DNA. Superoxide dismutase (SOD) is one of the primary antioxidants to inhibit free radicals. The purpose of this study was to determine the effect of ethanol extract of roselle calyx on SOD enzyme activity of the Sprague Dawley (SD) rats induced by 7.12-dimethylbenzen[a]anthracene (DMBA). The animals age of four weeks divided in to five groups, groups I were normal group. Group II is the negative control group only induced by DMBA 75 mg/kgBW; and group III ,IV , and V were treatment groups that treated by ethanolic extract of roselle calyx at dose of 10, 50 and 100 mg/kgBW/day for 7 days. After treatment with roselle, animals were induced by DMBA 75 mg/kgBW. On day 8 after DMBA induction, animals were fasted for 16 hours and blood was collected to measured SOD activity. Data were analyzed using One Way Analysis Of Variance (ANOVA) and LSD (P &lt;0.05). The result showed the significancies on increasing of SOD activity at 10 mg/kgBW (50.87±1.98), 50 mg/kgBW (69.98±3.58) and 100 mg/kgBW (73.01±6.95) compared with DMBA (43.74±1.95) (p&lt;0,05).

scholarly journals Antioxidant and Anti Inflammation Effect of Snack Bars from Black Glutinous Rice and Pumpkin Powder

2019 ◽  
Vol 2 (3) ◽  
pp. 77-88
Author(s):  
Akhmad Mustofa ◽  
Nanik Suhartatik ◽  
Ega Sulistiyo Ningrum
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Natural Medicine ◽  
Glutinous Rice ◽  
Pumpkin Powder

The study was design to evaluate the antioxidant and anti-inflammatory effect of snack bars that made of black glutinous rice and pumpkin powder and study on the male Sprague Dawley rats. Twenty-five male rats in between the age of 2 and 3 months were selected and randomly divided into 5 groups, which were positive control, negative control, group with sylimarin treatment, snack bars A and snack bars B. Carbon tetrachloride was used to induce hepatic damage. The results showed that group with CCl4 treatment has 3.04 ± 0.16 μmol/L MDA while group with Sylimarin, snack bars A, snack bars B were 0.49 ± 0.05; 0.54 ± 0.05; and 1.15 ± 0.16 μmol/L MDA, respectively. The snack bars had the capacity to decline the damage in liver as much as Sylimarin (standardized natural medicine for hepatotoxicity). The SGPT (Serum Glutamin Pyruvic Transaminase) of the two treatment was 22.24 ± 0.80 U/I for Sylimarin and 22.33 ± 1.03 U/I for snack bars while SGOT (Serum Glutamic Oxaloacetic Transaminase) was 40.78 ± 0.77 U/I for Sylimarin and 40.88 ± 1.25 U/I for snack bars A. Snack bars B has a significantly different value on final SGOT and SGPT.

scholarly journals The Effect of Ethanolic Extract of Roselle (Hibiscuss sabdariffa L) on Increasing SOD Activity

2016 ◽  
Vol 5 (1) ◽  
pp. 118
Author(s):  
Abdul Rahman W ◽  
Nurkhasanah Nurkhasanah ◽  
Nanik Sulistyani
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Activity Data ◽  
Group Iii

Free radicals were reactive species caused oxidation of lipids membrane and DNA. Superoxide dismutase (SOD) is one of the primary antioxidants to inhibit free radicals. The purpose of this study was to determine the effect of ethanol extract of roselle calyx on SOD enzyme activity of the Sprague Dawley (SD) rats induced by 7.12-dimethylbenzen[a]anthracene (DMBA). The animals age of four weeks divided in to five groups, groups I were normal group. Group II is the negative control group only induced by DMBA 75 mg/kgBW; and group III ,IV ,and V were treatment groups that treated by ethanolic extract of roselle calyx at dose of 10, 50 and 100 mg/kgBW/day for 7 days. After treatment with roselle, animals were induced by DMBA 75 mg/kgBW. On day 8 after DMBA induction, animals were fasted for 16 hours and blood was collected to measured SOD activity. Data were analyzed using One Way Analysis Of Variance (ANOVA) and LSD (P &lt;0.05). The result showed the significancies on increasing of SOD activity at 10 mg/kgBW (50.87±1.98), 50 mg/kgBW (69.98±3.58) and 100 mg/kgBW (73.01±6.95) compared with DMBA (43.74±1.95) (p&lt;0,05).

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

scholarly journals Multi Floral Honey Has a Protective Effect against Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in Sprague Dawley Rats

2017 ◽  
Vol 9 (2) ◽  
pp. 196 ◽  
Author(s):  
Hamed R. Takruri ◽  
Maha S. Shomaf ◽  
Saida F. Shnaigat
Keyword(s):  
Protective Effect ◽  
Mammary Cancer ◽  
Histopathological Examination ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Light Cycle ◽  
Sprague Dawley ◽  
Dark Light

This research was conducted to study the protective effect of bee honey on the 7,12-dimethylbenz(a)anthracene (DMBA)- induced breast cancer in rat model. The study consisted of three groups: honey group, positive control group (PC), and negative control group (NC) to which the carcinogen was not administered. All rats were fed the diet recommended by the American Institute of Nutrition for growing rats (AIN-93G), with addition of honey (50 g/kg diet) to the honey group. All Rats were fed their diets ad libitum on 12 hours dark/light cycle. At the age of 50 days all rats in the honey and PC groups were gavaged once by the carcinogen DMBA with a dose of 80 mg/kg body Wt. After three weeks of carcinogen administration, rats were palpated weekly to detect any tumor growth. After 18 weeks, all rats were sacrificed. The palpable structures and the mammary glands along with associated lymph nodes were removed and fixed in saline formalin and prepared for histopathological examination. The results revealed that the honey group diet significantly (p < 0.05) reduced the incidence rate of mammary cancer, palpable tumor multiplicity, tumor size and weight compared to the PC group. In conclusion, multi floral honey has a protective effect against DMBA- induced mammary cancer in the initiation, promotion, and progression stages of DMBA-induced mammary carcinogenesis. However, further research is needed to reveal the mechanisms that might have contributed to the preventive effect of honey against mammary cancer.

scholarly journals Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

2021 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
Alvyan Lantang Anugrah ◽  
Hana Eliyani ◽  
Budi Utomo ◽  
Suherni Susilowati ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Sertoli Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Beet Root ◽  
White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

scholarly journals ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

2018 ◽  
Vol 11 (4) ◽  
pp. 81
Author(s):  
Hanafis Sastra Winata ◽  
Rosidah Rosidah ◽  
Panal Sitorus
Keyword(s):  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Garcinia Xanthochymus ◽  
Anti Inflammatory

 Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

scholarly journals BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 225
Author(s):  
Sulaeman A ◽  
Patonah Patonah ◽  
Patonah Patonah ◽  
Negara Gg ◽  
Negara Gg
Keyword(s):  
Test Group ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Adipose Tissues ◽  
Obese Male

  Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

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