scholarly journals Multi Floral Honey Has a Protective Effect against Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in Sprague Dawley Rats

2017 ◽  
Vol 9 (2) ◽  
pp. 196 ◽  
Author(s):  
Hamed R. Takruri ◽  
Maha S. Shomaf ◽  
Saida F. Shnaigat
Keyword(s):  
Protective Effect ◽  
Mammary Cancer ◽  
Histopathological Examination ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Light Cycle ◽  
Sprague Dawley ◽  
Dark Light

This research was conducted to study the protective effect of bee honey on the 7,12-dimethylbenz(a)anthracene (DMBA)- induced breast cancer in rat model. The study consisted of three groups: honey group, positive control group (PC), and negative control group (NC) to which the carcinogen was not administered. All rats were fed the diet recommended by the American Institute of Nutrition for growing rats (AIN-93G), with addition of honey (50 g/kg diet) to the honey group. All Rats were fed their diets ad libitum on 12 hours dark/light cycle. At the age of 50 days all rats in the honey and PC groups were gavaged once by the carcinogen DMBA with a dose of 80 mg/kg body Wt. After three weeks of carcinogen administration, rats were palpated weekly to detect any tumor growth. After 18 weeks, all rats were sacrificed. The palpable structures and the mammary glands along with associated lymph nodes were removed and fixed in saline formalin and prepared for histopathological examination. The results revealed that the honey group diet significantly (p < 0.05) reduced the incidence rate of mammary cancer, palpable tumor multiplicity, tumor size and weight compared to the PC group. In conclusion, multi floral honey has a protective effect against DMBA- induced mammary cancer in the initiation, promotion, and progression stages of DMBA-induced mammary carcinogenesis. However, further research is needed to reveal the mechanisms that might have contributed to the preventive effect of honey against mammary cancer.

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

scholarly journals The Effect of Tempeh Gembus Variations to Serum Levels of High Sensitivity C-Reactive Protein (hsCRP) and Serum Levels of Fibrinogen of Sprague Dawley Rats with Aterogenic Diet

2018 ◽  
Vol 25 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Partika Kharunia Dewi ◽  
Diana Nur Afifah ◽  
Ninik Rustanti ◽  
Mohammad Sulchan ◽  
Gemala Anjani
Keyword(s):  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Atherogenic Diet ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Significant Difference

Abstract Background and aims: Cardiovascular diseases are widespread and causes many deaths in the world. The concentration of acute phase protein: C-reactive protein (CRP) and fibrinogen will rise dramatically when inflammation happens, which that can be used as an early marker of cardiovascular disease risk. Tempeh gembus contains fiber, unsaturated fatty acids and isoflavones are believed to reduce the inflammatory reaction. The aim of the study was to determinate the effect of tempeh gembus variations to levels of hcCRP and levels of fibrinogen of Sprague Dawley rats with atherogenic diet. Material and methods: This study was quasi-experimental with posttest only randomized control group design using 35 Sprague Dawley mice. The rats were randomized into 5 groups: negative control group given the standard diet, the positive control group given standard diet and atherogenic diet, and three treatment groups were given the standard diet, atherogenic diet and variation of tempeh gembus (tempeh gembus, heated tempeh gembus and tempeh gembus with bromelain enzyme) for 28 days. Serum levels of hsCRP and fibrinogen examined using ELISA (Enzyme-linked Immunosorbent Assay). Results and conclusions: The administration of tempeh gembus with bromelain enzyme is the most effective treatment for hsCRP serum level indicated a significant difference (p=0.028) between the negative control group, positive control group and first group with the third group. Fibrinogen serum levels showed significant differences in all treatment groups (p =0.042), administration of tempeh gembus with bromelain enzyme is the most effective treatment is shown by a significant difference between the negative control group and the positive control group with third group. The administration of tempeh gembus with bromelain enzyme for 28 days can reduce the serum levels of hsCRP and fibrinogen on rats significantly.

scholarly journals EFFICACY OF A STANDARDIZED ETHANOL EXTRACT OF ROSELLE CALYX IN THE TREATMENT OF ORAL MUCOSA ULCERATION (IN VIVO)

2017 ◽  
Vol 10 (17) ◽  
pp. 183
Author(s):  
Reni Jayantini ◽  
Dewi Fatma Suniarti ◽  
Agoeng T Sarwono
Keyword(s):  
Topical Application ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Ethanol Extracts ◽  
Inflammation Score

 Objectives: To analyze the effectiveness of the topical application of a standardized ethanol extract of roselle calyx in the treatment of oral mucous ulceration.Methods: Twelve Sprague Dawley rats were divided into two groups, with oral mucous ulcer being induced in all the rats. The ulcer diameter, reddish color, and the swollen membrane around the ulcer, as well as the inflammation score of the ulcer, were recorded for all animals. The negative control group was treated with aquades, while the positive control group was treated with 0.1% triamcinolone acetonide. The treatment group received the topical application of 7.5% and 15% standardized ethanol extract of roselle calyx twice a day for 3 or 7 days. On the 4th day, six rats were sacrificed, while the remaining six rats continued to receive treatment as before. On the 8th day, all the rats were sacrificed. Specimens were fixed, and histology slides were made. Further, microscopic slides were observed and scored.Result: Both the 7.5% and 15% standardized ethanol extracts of roselle calyx were found to reduce the ulcer diameter and inflammation score. No swelling or redness was observed.Conclusions: The application of 7.5% standardized ethanol extract of roselle calyx for 7 days is equally as effective as the application of 0.1% triamcinolone acetonides, except in terms of the decrease in the inflammation score.

scholarly journals Is traditional herbal mix composition effective on wound healing?

2021 ◽  
Vol 8 (1) ◽  
pp. 29-38
Author(s):  
Serap Gokce Eskin ◽  
Yucel Basimoglu Koca ◽  
Serdal Ogut
Keyword(s):  
Wound Healing ◽  
Negative Control Group ◽  
Grape Seed ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Wound Model ◽  
Experimental Group

Objective: Wound healing is a dynamic process that includes biochemical and physiological phenomena. Studies confirm that plants are used in wound healing to a great extent.  Antique oil (HBX 2371) has been produced as a traditional herbal mixed. This herbal mixture contains sesame oil, thyme oil, olive leaf, fig seed, grape seed, turmeric, and cinnamon. We evaluated traditional plant mixed oil ( Antique oil) for its wound-healing activity using an excision wound model in rats. Methods: The study used a preclinical, in vivo experimental, and analytical design. In the study, a total of 21 Sprague-Dawley rats, including 7 as experimental group, 7 as negative-control group, and 7 as positive-control group, were used. After the ischemic wound was created in experimental animals, the research data relating to the histochemical changes and biochemical parameters of the wound healing parameters were collected Results: A to research in experimental group fibroblast count, collagen density, fat cells, epithelization scores higher than the other groups and inflammatory cell density lower than the others. According to the results of the study, the highest TAC value (3.94 ± 0.21) was determined in the experimental group and results showed that antique oil administration decreased the TOC value. Conclusion: In conclusion, it was observed that antique oil prevented the wound changes induced by the incision, increased the repair of the epidermal and dermal structure in a short time, increased the antioxidant level, and decreased the oxidation level. Clinical studies are recommended.

scholarly journals THE ROLE OF VITAMIN E (α-TOCOPHEROL) ON TESTOSTERONE LEVEL IN SPRAGUE DAWLEY RATS FOLLOWING CISPLATIN TREATMENT

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Dian Kartika Rezia ◽  
Lukman Hakim ◽  
Wahjoe Djatisoesanto
Keyword(s):  
Vitamin E ◽  
Adult Male ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
White Rats ◽  
Sprague Dawley Rats

Objective: To observe the difference of testosterone levels in adult male Sprague Dawley rats treated with combination of cisplatin and vitamin E compared to those treated with cisplatin only. Material & Methods: We used 24 adult male Sprague Dawley rats weight 200–300 grams and randomly assigned into 4 groups (n=6). Rats in negative control group (NC) were given intraperitoneal normal saline injection, while the positive control (PC) group were injected with cisplatin 5 mg/kgBW at the end of the 3rd week. Two other groups, P1 and P2, were injected with cisplatin 5 mg/kgBW and given vitamin E orally 50 mg/kgBW and 200 mg/kgBW, respectively. Cardiac blood was aspirated at the end of the 7th week and processed for analysis of testosterone levels. Results: We recorded a significantly lower testosterone levels in rats treated only with cisplatin 5 mg/kgBW (CP) compared to those in CN group (p=0.006), and those receiving combination of cisplatin and vitamin E 50 mg/kgBW (p=0.003) and 200 mg/kgBW (p=0.001). Though not significant, testosterone levels were higher in P2 group than in P1 group (p=0.702). Conclusion: Exposure to cisplatin can lower testosterone levels in white rats, and the administration of vitamin E gives protection against such effect. 

scholarly journals Protective Effect of Ethanol Extract Tempuyung Leaf (Sonchus arvensis L.) Against Gentamicin Induced Renal Injury Viewed from Blood Ureum Level

2017 ◽  
Vol 1 (6) ◽  
Author(s):  
Imelda Imelda ◽  
Cherry Azaria ◽  
Teresa Lucretia
Keyword(s):  
Treatment Group ◽  
Protective Effect ◽  
Renal Injury ◽  
Filtration Rate ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Acute Renal Injury

Acute renal injury is characterized by decrement of glomerular filtration rate (GFR), resulting in nitrogen metabolic end product retention such as blood ureum. Aminoglycoside nephrotoxic injuries are common in elderly, with mortality rate 50-70%. Tempuyung has been empirically used in relieving renal problems as it contains several active antioxidants. This study aimed to analyze the protective effect of ethanol extract tempuyung leaf (EET) against gentamicin induced renal injury viewed from blood ureum level. The method used was true experimental laboratory, 16 wistar male rats, divided into 4 groups (n=4): negative control group (CMC), positive control group (Gentamicin 80 mg/kgBW), 1st treatment group (EET 100 mg/kgBW + Gentamicin), 2nd treatment group (EET 200 mg/kgBW + Gentamicin) for ten days. On the 11th day, blood serum were taken and the ureum level were analyzed. Data was analyzed by ANOVA and post hoc LSD test. Result showed positive control group had the highest blood ureum level (29.50  1.29 mg/dL) compared to negative control group (14.25  1.26 mg/dL), 1st treatment group (26.50  0.58 mg/dL), and 2nd treatment group (22.25  1.26 mg/dL) with significance difference (p<0,001). In conclusion, the result suggested the protective effect of EET against gentamicin induced renal injury.Keywords: acute renal injury, gentamicin, glomerular filtration rate, tempuyung, ureum

The Effects of Antibiotics Blended Chitosan Membranes on the Calvarial Critical Size Defect in Sprague Dawley Rats

2007 ◽  
Vol 342-343 ◽  
pp. 857-860
Author(s):  
Gyung Joon Chae ◽  
Sang Bae Lee ◽  
Ui Won Jung ◽  
Yong Keun Lee ◽  
Chong Kwan Kim ◽  
...  
Keyword(s):  
Critical Size ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Critical Size Defect ◽  
Chitosan Membrane ◽  
Calvarial Defects ◽  
Chitosan Membranes

The purpose of this study was to evaluate the osteogenesis of tetracycline blended chitosan membranes on the calvarial critical size defect in Sprague Dawley. An 8 mm surgical defect was created with a trephine bur in the area of the midsagittal suture. Forty rats were divided into four groups: negative control group, positive control group and two experimental groups. Three types of membranes were made and a comparative study was done. One type of non-woven membrane was made by chitosan for positive control. The other two types of non-woven membranes were made by immersing non-woven chitosan into either the tetracycline solution or the chitosan-tetracycline solution. Histologic analysis was done at 2 weeks and 8 weeks of healing periods. We concluded that that the use of tetracycline blended chitosan membrane on the calvarial defects in rats has a significant effect on the regeneration of bone tissue in itself. In addition it implicates that tetracycline blended chitosan membrane may be useful for guided tissue regeneration.

GASTRIC PROTECTION ABILITY OF SOME MEDICINAL MALAYSIAN PLANTS AGAINST ETHANOL INDUCTION MODEL IN SPRAGUE DAWLEY–RATS

2012 ◽  
Vol 57 (1) ◽  
Author(s):  
SUHAILAH WASMAN ◽  
MAHMOOD AMEEN ◽  
LEE SUAN CHUA ◽  
SALEHHUDDIN HAMDAN
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Strobilanthes Crispus ◽  
Mucosal Layer ◽  
Polygonum Minus ◽  
Ethanol Extracts

Ethanol extracts of different traditional Malaysian plants (Polygonum minus, Andrographis paniculata, Curcuma xanthorrhiza,Momordica charantia and Strobilanthes crispus) were evaluated for their gastroprotective activities. In this experiment several groups of Sprague Dawley rats weighing 200–250gm were used. The first group was pre treated with (0.25% w/v) carboxymethyl cellulose (negative control, 5 ml/kg), the second group was pre treated with 20 mg/kg omeprazole (positive control) and the other groups were pretreated with the selected Malaysian medicinal plants in two different doses 250 and 500 mg/kg respectively. After 60 min all the rats were treated with 95% ethanol for ulcer induction. Rats were sacrificed 1 h after induction and the stomachs were analyzed for gross and histological changes. Negative control group showed extensive lesions of gastric mucosal layer, whereas rats pretreated with omeprazole and Malaysian plants showed significant and dose dependent reduction in gastric lesions. Amongst, the selected plants Polygonum minuspotentially promote better inhibition percentage of ulcer area. So that we can conclude that Polygonum minus could be the selective plant as an antiulcerogenic agent for the treatment of gastric ulcer.

scholarly journals Antioxidant and Anti Inflammation Effect of Snack Bars from Black Glutinous Rice and Pumpkin Powder

2019 ◽  
Vol 2 (3) ◽  
pp. 77-88
Author(s):  
Akhmad Mustofa ◽  
Nanik Suhartatik ◽  
Ega Sulistiyo Ningrum
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Natural Medicine ◽  
Glutinous Rice ◽  
Pumpkin Powder

The study was design to evaluate the antioxidant and anti-inflammatory effect of snack bars that made of black glutinous rice and pumpkin powder and study on the male Sprague Dawley rats. Twenty-five male rats in between the age of 2 and 3 months were selected and randomly divided into 5 groups, which were positive control, negative control, group with sylimarin treatment, snack bars A and snack bars B. Carbon tetrachloride was used to induce hepatic damage. The results showed that group with CCl4 treatment has 3.04 ± 0.16 μmol/L MDA while group with Sylimarin, snack bars A, snack bars B were 0.49 ± 0.05; 0.54 ± 0.05; and 1.15 ± 0.16 μmol/L MDA, respectively. The snack bars had the capacity to decline the damage in liver as much as Sylimarin (standardized natural medicine for hepatotoxicity). The SGPT (Serum Glutamin Pyruvic Transaminase) of the two treatment was 22.24 ± 0.80 U/I for Sylimarin and 22.33 ± 1.03 U/I for snack bars while SGOT (Serum Glutamic Oxaloacetic Transaminase) was 40.78 ± 0.77 U/I for Sylimarin and 40.88 ± 1.25 U/I for snack bars A. Snack bars B has a significantly different value on final SGOT and SGPT.

The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 34
Author(s):  
Arya Barahmanta ◽  
Muhammad Faizal Winaris ◽  
Pambudi Raharjo
Keyword(s):  
Hyperbaric Oxygen ◽  
Oxygen Therapy ◽  
Tooth Movement ◽  
Bone Remodelling ◽  
Orthodontic Tooth Movement ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Pressure Area

<p><strong><em>Background:</em></strong><em> Orthodontic tooth movement is a </em><em>interaction prosess</em><em> of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO).  <strong>Purpose: </strong>To determine the difference number  of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. <strong>Materials and Methods</strong>: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male)  were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. <strong>Result:</strong> The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than  negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest  than positive control group (K+=3,83) and treatment (P=3,25). <strong>Conclusion: </strong>Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.</em></p><pre><strong> </strong></pre><p><strong><em>Keywords:</em></strong><em> Hyperbaric Oxygen, Tooth movement, Bone remodeling, </em><em>Osteocyte</em><em></em></p><p><em> </em></p><p><strong><em>Correspondence:</em></strong><em> </em><em>Arya Brahmanta</em><em>, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email:</em><em> </em><a href="mailto:[email protected]"><em>arya.brahmanta</em><em>@</em><em>hangtuah.ac.id</em></a></p>

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