scholarly journals YELLOW PITAYA (HYLOCEREUS MEGALANTHUS) FATTY ACIDS COMPOSITION FROM ECUADORIAN AMAZONIA

Author(s):  
Altuna Jl ◽  
Silva M ◽  
Alvarez M ◽  
Quinteros Mf ◽  
Morales D ◽  
...  

Objective: The aim of this study was to determine the composition of methyl esters fatty acids (FAMEs) in yellow pitaya (Hylocereus megalanthus) seeds cultivated in the Palora, Ecuador Amazonian region.Methods: Yellow pitaya oil was obtained from yellow pitaya seeds using the Soxhlet technique. FAMEs identification and quantification were carried out using the gas chromatography (GC) with mass spectrometry (MS) and the database Library NIST14.L to identify the FAMEs present in yellow pitaya oil.Results: Yellow pitaya oil from Ecuador Amazonian region was analyzed by GC-MS, to obtain the five main fatty acids, palmitic acid (11.52%), stearic acid (4.29%), oleic acid (11.09%), vaccenic acid (3.08%), and linoleic acid (69.98%). Omega 6 was the most abundant fatty acid, total content in yellow pitaya seeds oil.Conclusions: Yellow pitaya seeds content a good proportion of polyunsaturated fatty acids (omega 6). For their fatty acid composition, yellow pitaya seeds can be considered as healthy food and can be used in the food industry for different purposes. Regular consumption of yellow pitaya can improve human health.

Author(s):  
Altuna Jl ◽  
Silva M ◽  
Álvarez M ◽  
Quinteros Mf ◽  
Morales D ◽  
...  

Objective: The aim of this study was to determine the methyl esters fatty acids (FAMEs) profile of quinoa seeds (Chenopodium quinoa Willd) of a sample cultivated in Guaranda, Ecuador.Methods: Quinoa oil was obtained from quinoa seeds using the Soxhlet method. FAMEs identification and quantification were carried out using the gas chromatography (GC) with a mass spectrometry (MS), using the database Library NIST14.L to identify the fatty acids present in quinoa oil.Results: Quinoa oil from Ecuador was analyzed by GC-MS, to obtain four majoritarian fatty acids, palmitic acid (10.66%), oleic acid (24.70%), linoleic acid (62.47%), and linolenic acid (2.19%). Omega 6 was the most abundant fatty acid in quinoa oil. Quinoa oil has a good proportion of oleic acid and linoleic acid.Conclusions: Quinoa seeds present a good proportion of fatty acids. These seeds can be used in the food industry for different purposes to enjoy their fatty acids composition. Regular consumption of quinoa can improve health.


Author(s):  
Carrillo W ◽  
Carpio C ◽  
Morales D ◽  
Vilcacundo E ◽  
Álvarez M ◽  
...  

  Objective: The aim of this work was to determine the fatty acids content in corn seeds oil (Zea mays) sample cultivated in Ecuador.Methods: Corn oil was obtained from corn oil seeds using the cold pressing method. Methyl esters fatty acids analysis were carried out using the gas chromatography (GC) method with a mass selective detector and using the database library NIST 14.L to identify the compounds present in the corn seed oil.Results: Methyl esters fatty acids were identified from corn (Z. mays) seeds using the GC mass spectrometer (GC-MS) analytical method. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, elaidic acid, linoleic acid, arachidic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GS-MS method. Corn oil has a high content of linoleic acid (omega 6) with a value of 52.68% of the total content of fatty acids in corn oil and 29.70% of oleic acid (omega 9) of the total content of fatty acids in corn oil. The sample presented a value of 12.57% of palmitic acid.Conclusions: Corn oil shows a good content of fatty acids omega 6 and 9. The higher value was of omega 6 with 52.68% content. Corn oil has a good proportion of polyunsaturated of lipids (53.80%) and 14.86% of saturated lipids.


2020 ◽  
Vol 24 (5) ◽  
pp. 97-103
Author(s):  
A.N. Ivankin ◽  
◽  
A.N. Zarubinа ◽  
G.L. Oliferenko ◽  
A.S. Kuleznev ◽  
...  

The article discusses the scientific and technical issues of the methodology for producing liquid biofuel from renewable raw materials of plant origin. As raw materials used wastes from the production of pulp and paper mills — tall oil. The purpose of the work was to study the process of obtaining and determining ways to use the product. The optimal conditions for obtaining biodiesel are determined. The processing of raw materials was carried out by heating it with methanol in a ratio of 1:0,3 at a temperature of 50–65 °C for 2 hours in the presence of 2 % catalyst. The product, after separation of the resulting intermediate, was finally washed with water from the catalyst residues. The process was controlled spectrophotometrically. Using gas-liquid chromatography with mass spectrometric detection, the complete chemical composition of the used raw materials of various degrees of purification was established. It is shown that the main content in the processed oil is represented by natural lipids. Their fatty acid composition is described, comprising more than thirty C10–C24 fatty acids. A general scheme for producing biodiesel, which is a mixture of fatty acid alkyl esters, has been developed. The product was obtained by chemical transformation in the presence of acid catalysts, followed by the formation of fatty acid methyl esters. The component composition of the obtained product, biodiesel, was studied and it was shown that it consists of a mixture of methyl esters of fatty acids more than 95 %. In biodiesel, more than two hundred organic substances are also contained in the form of an insignificant amount of microimpurities. Their number fluctuated around 0,001 %. The basic physico-chemical characteristics of the obtained biodiesel are described in comparison with international requirements for biofuels. The analyzed product samples obtained from distilled TM, according to mass spectrometry, did not contain harmful impurities bromine, iodine, phosphorus and sulfur-substituted compounds. The total content of chlorine-substituted organic substances in all samples did not exceed 0,07 ± 0,02 %, and N-substituted derivatives did not exceed 0,05 ± 0,01 %, which indicates a rather high ecological purity of bitumen fuel. The main directions of the possible use of the product as liquid fuel for mini-boiler nozzles, as well as for operation in conventional diesel engines, are determined.


2013 ◽  
Vol 48 (5) ◽  
pp. 553-558 ◽  
Author(s):  
Luis Fernando Glasenapp de Menezes ◽  
Luciane Rumpel Segabinazzi ◽  
João Restle ◽  
Leandro da Silva Freitas ◽  
Ivan Luiz Brondani ◽  
...  

The objective of this work was to evaluate the meat lipid profile from Devon beef steers finished in pearl millet (Pennisetum americanum) pasture and fed at different rates of concentrate supplementary diet. Twelve steers weighing 270 kg, at 12‑month‑average initial age, were randomly distributed into three treatments: pearl millet pasture; and pearl millet pasture plus a concentrate equivalent at 0.5 or 1.0% of body weight, with two replicates. Total contents of saturated and unsaturated fatty acids, the polyunsaturated:saturated ratio and other relevant fatty acids as the vaccenic acid, conjugated linoleic acid, omega‑3, and omega‑6 were not affected by the consumption of a concentrate supplement at 0.5 or 1.0% live weight. However, the 0.5% supplementation level reduced the concentration of dihomo‑γ‑linolenic fatty acid (C20: 3 n‑6), while the 1.0% supplementation level elevated the content of docosahexaenoic (DHA) (C22: 6 n‑3) fatty acid, and the omega‑6:omega‑3 ratio in meat. Consumption of up to 1.0% energy supplementation increases the omega‑6:omega‑3 ratio in meat from Devon steers grazing on pearl millet pasture.


2016 ◽  
Vol 8 (2) ◽  
pp. 92
Author(s):  
Hamidou SENOU ◽  
Cai X. ZHENG ◽  
Gabriel SAMAKE ◽  
Mamadou B. TRAORE ◽  
Fousseni FOLEGA ◽  
...  

<p class="1Body">The methyl esters of fatty acids composition of the oil from <em>jatropha curcas</em> seeds were analyzed by gas chromatography-mass spectrometer GC-MS. Fourteen components were found to be representative with 99.52% of the total content of seed oils. The main constituents were unsaturated fatty acids (71.93%) and saturated fatty acids (27.59%). For the saturated fatty acids composition such as palmitic and stearic acid, the rate was 15.80% and 10.79%, respectively. Linoleic acid (39.58%) and oleic acid (30.41%) were obtained in highest concentration among the unsaturated fatty acids identified in the seeds oil of <em>Jatropha curcas</em> from Guizhou. This value also justifies the fluidity of the oil at room temperature. A high percentage of polyunsaturated fatty acids (39.58%) and a slightly lower rate of monounsaturated fatty acids (32.35%) were also observed. The seed oils profile of Guizhou <em>Jatropha curcas</em> presents the desirable fatty acid C14 to C18 and interesting features for the biodiesel production.</p>


2020 ◽  
Vol 4 (24) ◽  
pp. 152-160
Author(s):  
T.Ya. Prakhova ◽  
◽  
E.L. Turina ◽  
V.A. Prakhov ◽  
◽  
...  

Camelina sylvestris (L.) Crantz. is a valuable oilseed known due to the vegetable oil used in human nutrition and industrial production. The current research is aimed to assess the content of fatty acids in Camelina sylvestris oilseeds depending on the hydrothermal cultivation conditions. Seeds of winter camelina variety ‘Penzyak’ grown in the Penza region and steppe Crimea in 2017–2019 served as the object of the research. In 2017, arid conditions were recorded in the Crimea, Selyaninov Hydrothermal Coefficient (HTC) = 0.61. In the Penza region, on the contrary, the level of moisture in 2017 was moderate, HTC = 1.10. Weather conditions in 2019 were characterized as arid in both regions; HTC was 0.63 and 0.60. In 2018, in the Crimea and Penza, HTC was 0.23 and 0.45, respectively. The soils of the experimental plots – chernozems leached (Penza) and southern low-humic (Crimea). Fatty acid methyl esters were isolated and analyzed according to GOST R 51 486–99. The concentration of oleic and linoleic acids increased in seeds grown in the Crimea and amounted to 15.50–18.56 % and 18.08–19.58 %. In the colder region (Penza), the content of these acids decreased to 11.90–14.82 and 16.12–17.10 %, respectively. The highest content of linolenic acid was in oilseeds from the Penza region and amounted to 36.80–38.50 %, which was 4.94–6.40 % higher than the content of linolenic acid in oilseeds from the Crimea. The content of erucic acid in the Camelina sylvestris (L.) Crantz. from both regions was 2.81–2.94 %. The coefficient of variation of this acid was 1.59 %. Linolenic and oleic acids are the most susceptible to changes in cultivation conditions, coefficient of variation – 10.08 and 16.32 %, respectively. The total content of polyunsaturated fatty acids was high and averaged 52.4–54.6 %. The highest concentration was noted in oilseeds from Penza, which exceeded that of from the Crimea by 2.2 %. The content of ω-9 fatty acids ranged from 33.7 % (Penza) to 35.9 % (Crimea) and was characterized by a low coefficient of variation – 4.14 %. The range of variation of ω-3 and ω-6 acid, on average, was 6.32–7.81 %. Their content was 31.5 and 20.9 % in oilseeds from the Crimea and 35.5 and 19.1 % in oilseeds from Penza. The ratio between omega-6 and omega-3 acids is 1.8:1.


2014 ◽  
Vol 4 (1) ◽  
pp. 31-39
Author(s):  
Siwitri Kadarsih

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords : 


Author(s):  
E-Ming Rau ◽  
Inga Marie Aasen ◽  
Helga Ertesvåg

Abstract Thraustochytrids are oleaginous marine eukaryotic microbes currently used to produce the essential omega-3 fatty acid docosahexaenoic acid (DHA, C22:6 n-3). To improve the production of this essential fatty acid by strain engineering, it is important to deeply understand how thraustochytrids synthesize fatty acids. While DHA is synthesized by a dedicated enzyme complex, other fatty acids are probably synthesized by the fatty acid synthase, followed by desaturases and elongases. Which unsaturated fatty acids are produced differs between different thraustochytrid genera and species; for example, Aurantiochytrium sp. T66, but not Aurantiochytrium limacinum SR21, synthesizes palmitoleic acid (C16:1 n-7) and vaccenic acid (C18:1 n-7). How strain T66 can produce these fatty acids has not been known, because BLAST analyses suggest that strain T66 does not encode any Δ9-desaturase-like enzyme. However, it does encode one Δ12-desaturase-like enzyme. In this study, the latter enzyme was expressed in A. limacinum SR21, and both C16:1 n-7 and C18:1 n-7 could be detected in the transgenic cells. Our results show that this desaturase, annotated T66Des9, is a Δ9-desaturase accepting C16:0 as a substrate. Phylogenetic studies indicate that the corresponding gene probably has evolved from a Δ12-desaturase-encoding gene. This possibility has not been reported earlier and is important to consider when one tries to deduce the potential a given organism has for producing unsaturated fatty acids based on its genome sequence alone. Key points • In thraustochytrids, automatic gene annotation does not always explain the fatty acids produced. • T66Des9 is shown to synthesize palmitoleic acid (C16:1 n-7). • T66des9 has probably evolved from Δ12-desaturase-encoding genes.


2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
C. Castro-Correia ◽  
S. Sousa ◽  
S. Norberto ◽  
C. Matos ◽  
V. F. Domingues ◽  
...  

Context. Several studies have shown a link between proinflammatory activity and the presence or deficit of some fatty acids. Inflammation is associated with several diseases including diabetes.Objective. To characterize and compare the fatty acids profiles in children with inaugural type 1 diabetes, diabetic children (at least 1 year after diagnosis), and healthy children.Design. Plasma fatty acids profiles in children with inaugural diabetes, children with noninaugural diabetes, and controls, all of whom were prepubescent with a BMI < 85th percentile, were evaluated.Results. Omega-3 fatty acid levels were higher in recently diagnosed subjects with diabetes than in controls. The ratio of omega-6/omega-3 fatty acids was higher in the control population. Omega-6 fatty acid levels were higher in the nonrecent diabetic subjects than in the children with recently diagnosed diabetes, and the levels were higher in the nonrecent diabetes group compared to the control group.Conclusion. Our findings showed higher levels of alpha-linolenic acid, EPA, and DHA, as well as mono- and polyunsaturated fatty acids, in diabetic children. These findings reinforce the importance of precocious nutritional attention and intervention in the treatment of diabetic children.


Agriculture ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 189 ◽  
Author(s):  
Maria Salvatore ◽  
Selene Giambra ◽  
Daniele Naviglio ◽  
Marina DellaGreca ◽  
Francesco Salvatore ◽  
...  

There is evidence that secondary metabolites are involved in the fungal pathogenicity and virulence of Neofusicoccum spp. Fatty acids may also influence the plant–pathogen interaction but, so far, no information is available on their production by species of Neofusicoccum associated with Botryosphaeria dieback, which is a well-known syndrome of several plants with a complex etiology. In the present paper, the production of fatty acids in liquid medium, by strains of N. vitifusiforme and N. parvum associated with declining Sicilian vine plants, was evaluated. Data, acquired via gas chromatography–mass spectrometry (GC/MS), show the presence of linoleic acid as the most abundant fatty acid produced by both examined strains. In addition, the pathogenicity of N. vitifusiforme was tested on 2-year-old grapevine plants of cv. Inzolia.


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