scholarly journals IN VITRO α-AMYLASE AND Α-GLUCOSIDASE INHIBITOR ACTIVITIES OF ALBIZIA PROCERA STEM BARK

2018 ◽  
Vol 11 (9) ◽  
pp. 344
Author(s):  
Anand D ◽  
Sathish M ◽  
Dhivya Ls
Keyword(s):  
Inhibitory Activity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Butanol Fraction ◽  
Glucosidase Inhibitor ◽  
Dose Dependent ◽  
Albizia Procera

Objective: This current study was carried out to determine the in vitro α-amylase and α-glucosidase inhibitory activity of extract and fractions of Albizia procera. Methods: The α-amylase and α-glucosidase inhibition assay was carried out at concentrations 50–2000 μg/ml and acarbose used as standard. The absorbance was measured at 540 nm and recorded by spectrophotometer. Percentage inhibition was calculated for both the assays. Preliminary phytochemical screening was also evaluated using standard procedures.Results: There was a dose-dependent percentage inhibition of extracts (petroleum ether and ethanol) and fractions (chloroform, ethyl acetate, and n-butanol). The ethanol extract and n-butanol fraction show good inhibitory activity against both α-amylase and α-glucosidase with the percentage inhibition of 86.20% and 88.20% and 83.13% and 87.10%, respectively. The preliminary phytochemical screening shows that ethanol extract consists of active constituents such as flavonoids and phenolic compounds and tannins.Conclusion: This finding suggests that the ethanol extract and n-butanol fraction show good inhibitory activity against both α-amylase and α-glucosidase and show good antidiabetic activity.

scholarly journals COMPARISON OF α –GLUCOSIDASE INHIBITORY ACTIVITY OF Moringa oleifera ETHANOLIC EXTRACT

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S269-S273
Author(s):  
Rizky Rahmwaty Alami ◽  
◽  
Herlina Rante ◽  
Yulia Yusrini Djabir ◽  
◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Glucosidase Inhibitor ◽  
Plant Samples

The purpose of this research was to determine the α-glucosidase enzyme inhibitory activity of Moringa oleifera plant samples collected from the three geographical areas viz., Saragi, Bacuhau, and Batumatongka of Southeast Sulawesi Indonesia. Ethanol extract of Moringa leaves was prepared by the maceration method using 95% ethanol. The estimation of α –glucosidase inhibitory activity of this extract was performed in vitro. The results of the study showed that ethanolic extract of three Moringa samples i.e. Sarangi, Bacuhau, and Batumatongka had the IC50value of 18.62, 10.18, 10.58 ppm, respectively while IC50value for the acarbose positive control was reported 11.54ppm. From the results of this study, it can be concluded that ethanolic extract of Moringa could inhibit α –glucosidase and this potential was similar to the commercial α –glucosidase inhibitor acarbose.

scholarly journals In Vitro Anthelmintic Activity of Ethanol Stem Bark Extract of Albizia ferruginea (Guill. & Perr.) Benth

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Miriam Tagoe ◽  
Yaw Duah Boakye ◽  
Theresah Appiah Agana ◽  
Vivian Etsiapa Boamah ◽  
Christian Agyare
Keyword(s):  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Motility Assay ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Methanol Fraction ◽  
Test Concentration ◽  
Stem Bark Extract

Albizia ferruginea (Guill. & Perr.) Benth bark is used in the traditional medicine as a vermifuge. This study sought to determine the anthelmintic activity of the stem bark extract of Albizia ferruginea. The powdered A. ferruginea stem bark was extracted with ethanol. Phytochemical screening was carried out on A. ferruginea ethanol extract (AFE) and then screened for its anthelmintic property against Pheretima posthuma and Haemonchus contortus using the adult motility assay. The effect of AFE and its fractions on the anthelminthic activity of mebendazole and albendazole were also determined using the adult worm (P. posthuma) motility assay. AFE showed a dose-dependent anthelmintic activity against P. posthuma and H. contortus. The least concentration of AFE (0.5 mg/mL) paralyzed and killed P. posthuma within 272.50 ± 12.42  min and 354.50 ± 5.06  min of exposure, respectively. AFE at the least test concentration (0.14 mg/mL) caused paralysis and induced death of H. contortus, after at 63.50 ± 2.98 and 254.96 ± 2.44  min of exposure, respectively. AFE extract at 0.25 and 0.125 mg/mL increased the paralytic and helminthicidal activities of albendazole. The paralytic and helminthicidal activities of mebendazole were reduced when combined with AFE (0.25 and 0.125 mg/mL). Among the three fractions obtained from AFE, the methanol fraction showed the highest anthelmintic activity. The methanol fraction at 0.5 mg/mL caused paralysis after 69.90 ± 0.15  min and death of worm after 92.53 ± 0.74  min of exposure. The petroleum ether and ethyl acetate fractions showed relatively low anthelmintic activity. Phytochemical screening of AFE revealed the presence of tannins, saponins, glycosides, alkaloids, and coumarins. The results from this study show that A. ferruginea possesses anthelmintic activity which gives credence to its folkloric use.

scholarly journals Antidiabetic Activity of Terminalia sericea Constituents

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Nolitha Nkobole ◽  
Peter James Houghton ◽  
Ahmed Hussein ◽  
Namrita Lall
Keyword(s):  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Vero Cells ◽  
Stem Bark ◽  
Psidium Guajava ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Traditional Use ◽  
Sclerocarya Birrea

Diabetes mellitus is an endocrine disorder that affects more than 100 million people worldwide. South African plants namely Terminalia sericea, Euclea natalensis, Warbugia salutaris, Aloe ferox, Artemisia afra, Sclerocarya birrea, Spirostachys africana and Psidium guajava were investigated for their in vitro α-glucosidase and α-amylase properties, and antioxidant activities. Terminalia sericea stem bark extract showed the best results against α-glucosidase and α-amylase enzymes. Bioassay-guided fractionation of an acetone extract of T. sericea stem bark led to the isolation of four known compounds namely β-sitosterol (1), β-sitosterol-3-acetate (2), lupeol (3), and stigma-4-ene-3-one (4), in addition to two inseparable sets of mixtures of isomers [epicatechin-catechin (M1), and gallocatechin-epigallocatechin (M2). 1 and 3 showed the best inhibitory activity on α-glucosidase (IC50:54.5 and 66.5 μM). Bio-evaluation of the inhibitory activity of the purified compounds on α-amylase showed that 3 and 1 exhibited IC50 values of 140.7 and 216.02 μM, respectively against α-amylase. Compounds 2, M1, 3 and M2 were found to be nontoxic to Vero cells. This study is the first to report α-glucosidase and α-amylase activity of M1, M2, 2 and 4 isolated from T. sericea, which validated the traditional use of the bark of T. sericea for diabetes in South Africa.

scholarly journals EVALUATION OF IN VITRO ANTIDIABETIC AND ANTIOXIDANT POTENTIAL OF BARLERIA CRISTATA LEAVES EXTRACTS

2018 ◽  
Vol 11 (4) ◽  
pp. 287
Author(s):  
Sakthivel Vasanth ◽  
Giridharan Bupesh ◽  
Tharumasivam Siva Vijayakumar ◽  
Vellingiri Balachandar ◽  
Durai Rajan Gunasekaran
Keyword(s):  
Antioxidant Activity ◽  
Petroleum Ether ◽  
Leaf Extract ◽  
Medical Condition ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Potential Activity ◽  
Dose Dependent

 Objectives: The objectives of the study were to study the in vitro antidiabetic and antioxidant activity of Barleria cristata leaves extracts.Methods: Ethanol extract and petroleum ether extract of leaf and root of B. cristata were tested for their antidiabetic activity. The in vitro antidiabetic activity from α-amylase, α-glucosidase, and antioxidant activity was used to assess the potential activity of the fractions.Results: Our attempt results suggest that ethanol and the petroleum ether leaf extract from B. cristata show signs of dose-dependent increases to inhibitory activity on α-amylase, α-glucosidase enzymes, and antioxidant activity when compared with control. Ethanolic leaves to extract produced maximum in vitro antidiabetic effect and antioxidant activity when compared to petroleum ether extract.Conclusion: The ethanol leaf extract from B. cristata may be used in managing of blood glucose in a medical condition like diabetes.

scholarly journals ANTIDIABETIC POTENTIAL OF THE OYSTER MUSHROOM PLEUROTUS FLORIDA (MONT.) SINGER

2017 ◽  
Vol 9 (4) ◽  
pp. 51
Author(s):  
Prabu M. ◽  
Kumuthakalavalli R.
Keyword(s):  
Inhibitory Activity ◽  
Tamil Nadu ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Pleurotus Florida ◽  
Dose Dependent

Objective: The present investigation comprises, in vitro antidiabetic activity such as α-amylase and α-glucosidase inhibitory activities and in vivo antidiabetic activity of methanolic extract of Pleurotus florida.Methods: The fruiting bodies of Pleurotus florida were obtained from Mushroom Unit, Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu, India. Sample preparation, qualitative phytochemical analysis, in vitro antidiabetic activities namely α-amylase and α-glucosidase inhibitory activity and in vivo antidiabetic activity namely evaluation of alloxan induced diabetic rats were carried out following the methods reported previously.Results: In vitro and in vivo antidiabetic activity of P. florida exhibited significant results for its α-amylase (94.93±1.75 % at 1000 µg/ml) and α-glucosidase inhibitory activity (84.90±0.42 % at 1000 µg/ml) in a dose-dependent manner. The extract also showed significant antidiabetic activity on in vivo (p<0.05) at the tested dose level (200 mg/kg b. w) this was comparable to Glibenclamide, a standard antidiabetic drug.Conclusion: The presence of phytochemicals namely phenols, flavonoids, saponins, tannins and terpenoids may be responsible for such antidiabetic activity. These results reveal that P. florida can be used as a potential antidiabetic agent.

scholarly journals Antidiabetic Activity of Extract Combination of Orthosiphon aristatus and Oryza sativa L. var glutinosa

2021 ◽  
Vol 4 (3) ◽  
pp. 202-209
Author(s):  
Eris Septiana ◽  
Nurul Maulida Rizka ◽  
Yadi Yadi ◽  
Partomuan Simanjuntak
Keyword(s):  
Biological Activity ◽  
Oryza Sativa ◽  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Oryza Sativa L ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Ic50 Value

Traditionally and scientifically, research has shown that Orthosiphon aristatus and Oryza sativa L. var. glutinosa have antidiabetic activity. The combination of two medicinal plants can increase their biological activity. This study aimed to determine the antidiabetic activity of O. aristatus and O. sativa L. var. glutinosa on single and combined extracts. Phytochemical screening of the single extract was done qualitatively. The α-glucosidase inhibitory method was used as an antidiabetic activity. The results showed that every extract contained alkaloids, steroids/triterpenoids, flavonoids, tannins, quinones, and coumarins. A single extract of O. sativa L. var glutinosa, O. aristatus, and their combinations (1:1, 1:2, and 2:1) had an α-glucosidase enzyme inhibitory activity with an IC50 value of 67.82, 80.93, 73.81, 88.72, and 61.51 µg/ml, respectively. The combination shows that the ratio of 1:1 was nearly additive, 1:2 was slight to moderate antagonism, and 2:1 was moderate to slight synergism. The combination of 96% ethanol extract of O. sativa L. var. glutinosa and O. aristatus in a ratio of 2:1 was the most effective in increasing its inhibitory activity.

scholarly journals SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIDIABETIC ACTIVITY OF BAUHINIA RACEMOSA LAM. LEAVES

2018 ◽  
Vol 11 (6) ◽  
pp. 190
Author(s):  
Bhimraj Gawade ◽  
Mazahar Farooqui
Keyword(s):  
Ethanol Extract ◽  
Mass Spectrometry Analysis ◽  
Antidiabetic Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Spectrometry Analysis ◽  
Ic50 Value ◽  
Inhibitory Potential

Objective: In this study, the leaves of medicinal plant Bauhinia racemosa Lam. with different pharmacological activities were subjected to phytochemical screening and assessment of their in vitro inhibitory potential with porcine pancreatic α-amylase enzyme to treat and management of diabetes.Methods: Plant leaves were extracted sequentially with ethanol solvent. A modified 3,5-dinitrosalicylic acid method was adopted to screen α-amylase inhibition assay. The ethanol extract was analyzed qualitatively and gas chromatography–mass spectrometry analysis technique for the active phytoconstituents according to the standard protocols.Results: A phytochemical screening of leaves extract reveals the presence of carbohydrate, alkaloids, saponin, glycosides, steroids, tannins, flavonoids, triterpenoid, and phenolic compounds. The ethanol extract reported inhibition of α-amylase enzyme activity at IC50 value 61.72 ± 0.03 μg/mL and acarbose as a standard drug at IC50 value 28.07 ± 0.02 μg/mL.Conclusion: The results of the study indicate that B. racemosa Lam. leaves contain some of bioactive phytochemicals might to be exhibiting in vitro antidiabetic activity, which was leading to decreases the rate of starch digestion.

scholarly journals in vitro Antidiabetic, Antioxidant and Cytotoxic Activities of Syzygium cumini Fractions from Leaves Ethanol Extract

2019 ◽  
Vol 10 (1) ◽  
pp. 24
Author(s):  
Nina Artanti ◽  
Faiza Maryani ◽  
Rizna Triana Dewi ◽  
Sri Handayani ◽  
Indah Dwiatmi Dewijanti ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Column Chromatography ◽  
Plant Extract ◽  
Inhibitory Activity ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Cytotoxic Activities ◽  
Syzygium Cumini

Ethanol extract of Syzygium cumini (jamblang) leaves reveal its significant α-glucosidase inhibitory activity which perform the potential activity of this plant extract as antidiabetic agent. However, other bioactivities of this plant extract have been reported. The aim of current study was to evaluate the comparison of in vitro antidiabetic, antioxidant and cytotoxic activity of S. cumini fractions. The S. cumini leaves ethanol extract was fractionated by using column chromatography with n-hexane, ethyl acetate and methanol as eluents. Seven fractions were obtained from column chromatography (F1-7). The results showed that the best antidiabetic activity was found in F5 (93% α-glucosidase inhibitory activity), the best antioxidant activity was found in F4 (83% inhibition of DPPH free radical) and the best cytotoxic activity was found in F2 (69% growth inhibition of T47D breast cancer cell line). Therefore besides in vitro antidiabetic activity, fractions of S. cumini leaves ethanol extract also showed antioxidant and cytotoxic activities. Since the results showed that the most active fractions for antidiabetic, antioxidant and cytotoxicity were differed, it could be possible to isolate the different potential active compounds for each activity.Keywords : Syzygium cumini, α-glucosidase inhibitor, DPPH, cytotoxicity

scholarly journals In VitroAnti-Plasmodial Activity ofTrigonella foenum–graecumL.

2010 ◽  
Vol 7 (4) ◽  
pp. 441-445 ◽  
Author(s):  
M. Palaniswamy ◽  
B. V. Pradeep ◽  
R. Sathya ◽  
J. Angayarkanni
Keyword(s):  
Ethanol Extract ◽  
Traditional Healers ◽  
Active Principle ◽  
Test Plant ◽  
Moderate Activity ◽  
Inhibition Assay ◽  
Phytochemical Screening ◽  
Solvent Systems ◽  
Fenugreek Leaves

Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. For the present study,Trigonella foenum-graecumL. (fenugreek) were collected from Coimbatore, Tamilnadu, India. The test plant has been used in India by traditional healers for the treatment of fever as well as other diseases. The active principle was extracted out in different solvent systems to assess the anti-plasmodial potential, with an aim that they can further be utilized to formulate drugs.In vitroanti-plasmodial assay of the extracted fractions of fenugreek leaves was carried out using laboratory adapted chloroquine sensitive and resistantPlasmodium falciparumisolates. Schizont maturation inhibition assay was adopted to analyze the potential of the extracts. Ethanol extract (50%) seemed to possess profound anti-plasmodial activity with IC50value of 8.75 ± 0.35 µg ml−1and 10.25 ± 0.35 µg ml−1against chloroquine sensitive and resistantP. falciparumisolates, respectively. Among the investigated six fractions of the plant extracts, two were found to have significant anti-plasmodial activity with IC50values <10 µg ml−1, namely ethanol and butanol extracts. Two extracts chloroform and ethyl acetate showed moderate activity with IC50values ranging from 10 to 20 µg ml−1, and the other two extracts, hexane and water appeared to be inactive with IC50values >85 µg ml−1. In addition, preliminary phytochemical screening of the various extracts indicated the presence of alkaloids, saponin, tannin like phenolic compounds, flavonoids and steroids.

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