scholarly journals COLOR INTENSITY AND ANTIOXIDATIVE CAPACITY OF FOUR NATURAL MONOFLORAL HONEY FROM MALDA, INDIA

2019 ◽  
pp. 111-115
Author(s):  
SURAJIT ROY ◽  
SHYAMAPADA MANDAL
Keyword(s):  
Phenolic Content ◽  
Color Variation ◽  
Total Phenolic ◽  
Antioxidative Capacity ◽  
Bioactive Components ◽  
Color Intensity ◽  
Potential Source ◽  
Ic50 Values ◽  
Total Flavonoids Content ◽  
Dark Color

Objective: The objective of the study was to explore the color intensity and antioxidative capacity of mango honey available from local niches, in and around Malda, West Bengal, India. Methods: Four mango honey samples: LMH1, LMH2, LMH3, and LMH4, were collected from bee hives, in and around Malda, India, and were subjected to determine the color intensity and antioxidative capacity in 2, 2-diphenyl-1-picrylhydrazyl system. Results: The honey samples tested had light to dark color variation with color intensity values of 583.59–1842.84 mAU. The total phenolic content (TPC) of the honey samples ranged from 434.52 to 1450.74 μg gallic acid equivalents/ml, while the total flavonoids content (TFC) ranged 39.4–72.67 mg quercetin equivalents/100 g of honey. The honey samples displayed antioxidative activity (AOA) with 9.91–76% inhibition capacity, and the IC50 values of 8.37–19.38 mg/ml. The color intensity of test honeys displayed positive correlation with the TPC (r=0.907) and TFC (r=0.863), while negative correlation between honey color intensity and IC50 values (r=−0.955) was recorded. Conclusion: The locally available mango honeys were rich in bioactive components displaying excellent AOA, and thus, the native honey might be utilized as the potential source of natural antioxidant in our part of the globe.

scholarly journals Potensi limbah kulit buah Nyirih Xylocarpus granatum sebagai inhibitor tirosinase

DEPIK ◽  
2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Mohamad Gazali ◽  
Neviaty P. Zamani ◽  
Irmanida Batubara
Keyword(s):  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Inhibition Rate ◽  
Inhibitory Effects ◽  
Fruit Peel ◽  
Potential Source ◽  
Xylocarpus Granatum ◽  
Ic50 Values ◽  
Peel Extract

The aim of the present study was to analyse the peel waste of Xylocarpus granatum fruits as potential source of tyrosinase inhibitors. Dried peel samples were ground to yield a powder (simplicia). Subsequently, they were extracted with organic solvents of distinct polarity levels, namely n-hexane (non-polar), chloroform (semi-polar) and methanol (polar) by use of the single-maceration method. Inhibitory effects on tyrosinase activity (monophenolase) and DOPA auto-oxidation (diphenolase) were determined in bioassays. Assays with the methanol extract revealed IC50 values of 784.87 μg mL-1 (monophenolase) and of 1176.66 μg mL-1 (diphenolase), respectively. In contrast, n-hexane and chloroform extracts showed no activity. These results indicate that the methanolic fruit peel extract contained tyrosinase-inhibiting compounds, such as flavonoids, tannins and saponins, whereas the n-hexane and chloroform extracts yielded alkaloids, steroids and triterpenoids without tyrosinase-inhibiting activity. The phenolic compounds had a strong effect on the tyrosinase enzymes, inhibiting monophenolases by 97% and diphenolases by 96%, with a positive correlation between the total phenolic content and the inhibition rate in both activities.

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

scholarly journals Variation of Bioactive Compounds and Antioxidant Activity of Jujube (Ziziphus jujuba) Fruits at Different Stages of Ripening

2018 ◽  
Vol 46 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Gheorghe ACHIM ◽  
Mihai BOTU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ripening Stage ◽  
Total Flavonoids Content ◽  
Ziziphus Jujuba

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100 g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human’s healthy eating.

Fining of red wines: effects on their analytical and sensory parameters

OENO One ◽  
2011 ◽  
Vol 45 (1) ◽  
pp. 47 ◽  
Author(s):  
Asimenia Karamanidou ◽  
Stamatina Kallithraka ◽  
Efimia Hatzidimitriou
Keyword(s):  
Phenolic Content ◽  
Practical Interest ◽  
Quality Parameters ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Red Wines ◽  
Grape Variety ◽  
Color Intensity ◽  
Wine Production ◽  
Fining Agent

<p style="text-align: justify;"><strong>Aim</strong>: The aim of the present study was to examine the influence of protein fining on selected quality parameters of wines made from indigenous Hellenic red grape varieties.</p><p style="text-align: justify;"><strong>Methods and Results</strong>: Three different commercial hydrolyzed gelatins and egg albumin were added to two young red wines at three concentrations commonly used in winemaking. The cultivars selected were Hellenic native <em>V. vinifera</em> species used for the production of high quality Appellation of Origin wines (Agiorgitiko and Xinomavro). All the quality parameters studied (anthocyanin concentration, color intensity and hue, ionization degree, total phenolic content, DPPH radical scavenging activity, tannin and polysaccharide concentration, gelatin and HCl indexes, as well as individual phenolic content) were significantly decreased after the addition of the fining agents. The decrease observed was mainly dependent on grape variety, which genetically determines the wine’s phenolic composition, and to a lesser extent on the fining agent used and the dosage applied. In general, treated wines obtained better scores in sensory analysis as compared to untreated ones, although statistically significant differences were only obtained in the Agiorgitiko wine regarding color intensity and hue, acidity, balance, aftertaste, and overall impression.</p><p style="text-align: justify;"><strong>Conclusion</strong>: Wine quality parameters after fining were mainly influenced by the wine’s initial phenolic composition, which is determined mostly by grape variety.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: The outcomes of such study might be of practical interest to winemakers since they could optimize red wine production technology by selecting the appropriate fining agent according to the specific phenolic profiles of the produced wines and thus improve their quality.</p>

scholarly journals AMINO ACID COMPOSITION AND ANTIOXIDANT PROPERTIES OF FIVE EDIBLE MUSHROOM SPECIES FROM KASTAMONU, TURKEY

2018 ◽  
Vol 15 (2) ◽  
pp. 80
Author(s):  
Temel kan Bakır ◽  
Mansor Boufars ◽  
Mertcan Karadeniz ◽  
Sabri Ünal
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Free Amino ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Edible Mushroom ◽  
Total Phenolic ◽  
Mushroom Species ◽  
Boletus Edulis ◽  
Ic50 Values

Background: Hydnum repandum, Cantharellus cibarius, Ramaria fennica, Boletus edulis, and Craterellus cornucopioides are all wild edible mushroom species from the Kastamonu province. The aim of this study was to investigate antioxidant properties and amino acid contents of these mushrooms. Materials and Methods: The mushrooms were analyzed for their free amino acid compositions using a high performance Amino Acid analyzer (Biochrom 30). Also, antioxidant activity and total phenolic concentrations of five different mushroom species were studied by using spectrophotometric methods. Results: The mushrooms contained 17 amino acids (g/100 g): Glutamic acid (2.56–1.11), Alanine (1.49–0.54), Arginine (1.62–6.77), Aspartic acid (1.45–0.81), Leucine (1.08–0.64), Methionine (1.05–0.06), Valine (1.05–0.66), Lysine (1.01–0.57), Serine (0.68–0.38), Cysteine (0.88–0.11), Isoleucine (0.61–0.39), Glycine (0.82–0.35), Threonine (0.82–0.44), Phenylalanine (0.66–0.42), Proline (0.60–0.47), Tyrosine (0.58–0.38), and Histidine (0.48–0.22). The total free amino acid (TAA) contents ranged from 169.2 mg/g in Boletus edulis to 89.1 mg/g in Hydnum repandum. These five different mushroom species contain eight essential amino acid species except tryptophan. The antioxidant activity of mushroom extracts was expressed as the percentage of DPPH radical inhibition and IC50 values (mg/mL). The percentage of inhibition ranged from 2.38% to 88.05% and IC50 values ranged from 0.03 to 13.98 mg/mL. The total phenolic content ranged from 0.66 to 7.74 mg/g of dry mushroom extract, expressed as gallic acid equivalents. Conclusion: Methanolic extract of Boletus edulis showed the highest phenolic content and strong antioxidant activity. As a result, the significant linear correlation between the values for the total phenolic content and antioxidant activity of mushroom extracts was corroborated in all of the investigated mushroom species, with the exception of Craterellus cornucopioides.

Antioxidant and antimicrobial capacities of ethanolic extract of Pergularia daemia leaves: a possible substitute in diabetic management

2016 ◽  
Vol 13 (3) ◽  
Author(s):  
Joseph Adusei Sarkodie ◽  
Sylvia Afriyie Squire ◽  
Emelia Oppong Bekoe ◽  
Charles Yaw Fosu Domozoro ◽  
Irene Awo Kretchy ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content

Abstract: The leaves of: The total phenolic content, total flavonoids content, radical scavenging activity and reducing power assays were estimated using Folin–Ciocalteu method, aluminium chloride colorimetric assay, Fe: The results showed that: These findings justify the folkloric use of

scholarly journals In-vitro antioxidant potential of the flavonoid glycosides from Cassia tora linn. Leaves

2016 ◽  
Vol 3 (1) ◽  
pp. 19-26
Author(s):  
A. Vijayalakshmi ◽  
S. Jayakumari ◽  
K. Masilamani
Keyword(s):  
Nitric Oxide ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Flavonoid Glycosides ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Cassia Tora ◽  
Ic50 Values

The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18 µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively.

scholarly journals ANTIBACTERIAL ACTIVITY OF TEA, BREADFRUIT, AND PIPER BETLE LEAVES EXTRACTS AGAINST Propionibacterium acnes AND ITS APPLICATION AS ANTI-ACNE MASK

2021 ◽  
Vol 18 (2) ◽  
pp. 105
Author(s):  
Angie Lucita ◽  
Tensiska Tensiska
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Phenolic Content ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Piper Betle ◽  
Bioactive Components ◽  
Disk Diffusion Method ◽  
Extract Concentration

Tea, breadfruit, and piper betle leaves contain bioactive components that have a function as an antibacterial. The purpose of this study was to determine the type of leaf extract that has the highest inhibition power against Proponibacterium acnes and determine the concentration of the selected extract for mask applications that have good physical characteristics and acceptable to panelists. Extraction of tea, breadfruit, and piper betel leaves were carried out with 96% ethanol solvent using maceration method for 24 hours and concentrated with a rotary evaporator. Thick extracts contribute to antibacterial activity by disk diffusion method and total phenolic content. The selected extract was formulated into a clay mask with concentration of 0.5%, 1%, and 1.5%. The result showed that breadfruit leaf extract had the highest inhibition power against Propionibacterium acnes which was 10.844 ± 0.327 mm (strong inhibition category) so that extract can be applied to clay mask. Breadfruit leaf extract concentration of 1.5% was the chosen formulation because it meets the standards, approved by the panelists and the most effective antibacterial for acne problems.

scholarly journals Aqueous Extract of Wild Cyclamen Tubers (Cyclamen Purpurascens L.) - A Potential Source of Natural Antioxidants and Antimicrobial Agents

2018 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Ljiljana P. Stanojević ◽  
Milorad D. Cakić ◽  
Jelena S. Stanojević ◽  
Dragan J. Cvetković ◽  
Bojana R. Danilović
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Antimicrobial Agents ◽  
Natural Antioxidants ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dry Extract ◽  
Potential Source ◽  
Total Flavonoids Content

Wild cyclamen tubers (Cyclamen purpurascens Mill.) (mountain Kukavica, Southeast Serbia) were used as material for extraction in this study. Aqueous extract was obtained by reflux extraction on boiling temperature with hydromodulus 1:20 m/v during 180 minutes. The total phenolic content was determined spectrophotometrically by the method of Folin-Ciocalteu, and the total flavonoids content by method with AlCl3. The antioxidant activity of extract was investigated spectrophotometrically by DPPH and ABTS test. Disc-diffusion method was used for antimicrobial activity investigation on the following pathogenic microorganisms: Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa and Klebsiella pneumoniae. The content of total phenols was 8.27 mg GAE/g dry extract while the total flavonoid content was 11.51 mg RE/g dry extract. The extract concentrations required to neutralize 50% of the initial concentration of DPPH radicals (EC50) after 20 minutes incubation and immediately after adding DPPH radical solution were 0.413 and 2.0 mg/ml, respectively, while concentrations of extract required to neutralize 50% of the initial ABTS radicals concentration is 0.743 mg/ml. The extract showed the highest antimicrobial activity on bacteria Staphylococcus aureus. The presented results indicate that cyclamen tubers extract is a potential source of natural antioxidants and antimicrobial agents.

scholarly journals Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel Xylaria Species Associated with Termite Nests and LC-MS Analysis

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1557
Author(s):  
Niwana Wangsawat ◽  
Lutfun Nahar ◽  
Satyajit D. Sarker ◽  
Cherdchai Phosri ◽  
Andrew R. Evans ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Specific Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Ic50 Values

Xylaria species associated with termite nests or soil have been considered rare species in nature and the few which have been reported upon have been found to act as a rich source of bioactive metabolites. This study evaluated 10 ethyl acetate extracts of five new Xylaria species associated with termite nests or soil for their antioxidant activity, and cytotoxicity against different cancer and normal cell lines. DPPH and ABTS radical scavenging activities of the extracts demonstrated strong capacity with low IC50 values. The highest observed activities belonged to X. vinacea SWUF18-2.3 having IC50 values of 0.194 ± 0.031 mg/mL for DPPH assay and 0.020 ± 0.004 mg/mL for ABTS assay. Total phenolic content ranged from 0.826 ± 0.123 to 3.629 ± 0.381 g GAE/g crude extract which correlated with antioxidant activities. The high total phenolic content could contribute to the high antioxidant activities. Cytotoxicity was recorded against A549, HepG2, HeLa and PNT2 and resulted in broad spectrum to specific activity depending on the cell lines. The highest activities were observed with X. subintraflava SWUF16-11.1 which resulted in 11.15 ± 0.32 to 13.17 ± 2.37% cell viability at a concentration of 100 µg/mL. Moreover, LC-MS fingerprints indicated over 61 peaks from all isolates. There were 18 identified and 43 unidentified compounds compared to mass databases. The identified compounds were from various groups of diterpenoids, diterpenes, cytochalasin, flavones, flavonoids, polyphenols, steroids and derivatives, triterpenoids and tropones. These results indicate that Xylaria spp. has abundant secondary metabolites that could be further explored for their therapeutic properties.

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