scholarly journals EFFICACY OF IONIC LIQUID [MIM]BR-BASED MAE ON RESVERATROL AND PHENOLIC COMPOUNDS EXTRACTION FROM GNETUM GNEMON SEEDS AND THEIR DPP-4 INHIBITORY ACTIVITY

2018 ◽  
Vol 10 (1) ◽  
pp. 168
Author(s):  
Arif Arrahman ◽  
Rezi Riadhi Syahdi ◽  
Hana Permatasari ◽  
Intan Fikri Purnama Sari ◽  
Abdul MunÍm
Keyword(s):  
Inhibitory Activity ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Screening Assay ◽  
Chromatography Method ◽  
Dipeptidyl Peptidase 4 ◽  
Gnetum Gnemon

Objective: This study aimed to determine the inhibitory activity of 1-butyl-3-methylimidazolium bromide ([bmim]Br) extracts of melinjo seeds(Gnetum gnemon) on dipeptidyl peptidase-4 (DPP-4).Methods: Melinjo seeds were extracted by a [bmim]Br microwave-assisted method using various extraction parameters and the inhibitory activityof DPP-4 of all extracts was determined in 96-well microplates using Cayman inhibitor screening assay. Determination of trans-resveratrol contentwas conducted using a reverse-phase high-performance liquid chromatography method. The total phenolic content was determined using a 96-wellmicroplate reader. Data analysis for the determination of the optimal extraction conditions was developed by response surface methodology.Results: The extract obtained from the third run showed the highest inhibition (28.73%) against DPP-4 activity with the total phenolic content of1.96 mg gallic acid equivalent/g the seed powder.Conclusion: The analytical results revealed the following optimal conditions: Solvent concentration 1.5 M, liquid-solid ratio 23:1, and extraction time15 min.

scholarly journals APPLICATION OF IMIDAZOLE-BASED IONIC LIQUIDS IN MICROWAVE-ASSISTED EXTRACTION OF TRANS-RESVERATROL FROM GNETUM GNEMON L. SEEDS

2018 ◽  
Vol 10 (1) ◽  
pp. 39
Author(s):  
Arif Arrahman ◽  
Andini Gahayati ◽  
Dewi Rizky ◽  
Satya Muslimah ◽  
Abdul Mun’im
Keyword(s):  
Ionic Liquids ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Chromatography Method ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Gnetum Gnemon

Objective: This study aimed to compare the efficiency of three imidazole-based ionic liquids (ILs) in the microwave-assisted extraction (MAE) oftrans-resveratrol from Gnetum gnemon seeds.Methods: Trans-resveratrol was quantitatively extracted using 1-hexyl-3-methylimidazolium bromide ([hmim]Br), 1-butyl-3-methylimidazoliumchloride ([bmim]Cl), and 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) in a microwave oven. Extraction parameters, including solventconcentration, liquid-solid ratio, and extraction time, were optimized using response surface methodology on the basis of the Box–Behnken design.A reverse-phase high-performance liquid chromatography method was used to determine resveratrol content with acetonitrile: water (75:25 v/v) asthe mobile phase. The total phenolic content was determined by the Folin–Ciocalteu method.Results: Under optimal conditions, the resveratrol content when extraction was performed using [hmim]Br, [bmim]Cl, and [bmim]BF4 was 0.068,0.059, and 0.038 mg/g dry weight, respectively, and total phenolic content was 0.647, 1.736, and 1.436 mg gallic acid equivalent/g, respectively.Conclusions: IL-based MAE using [hmim]Br provided the highest trans-resveratrol yield from melinjo seeds and can be used as an alternative methodfor this purpose.

scholarly journals Determination of Antioxidant Activity of Selected Vegetables Grown in Iran under Reversed Phase Conditions with UPLC

2015 ◽  
Vol 9 (5) ◽  
pp. 122-125
Author(s):  
Nahid Rastakhiz ◽  
Fereshteh Khosravi ◽  
Seyyed Sina Seyyed Jafari Olia ◽  
Behzad Iranmanesh
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Reversed Phase ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reverse Phase

This study describes the determination of some phenolic compounds in four different vegetables of Kerman in Iran including Carrots, Celery, Lettuce and red cabbage .The  phenolic compounds analyzed were (Ascorbate, Ferulic acid, Naringin) using reverse phase high performance liquid chromatography (RPUPLC). The results of analysis showed in vegetables ranged between 90 and 1080mg /kg. A high and significant correlation between antioxidant activity and total phenolic content was determined in vegetables (r2 = 0.9461 P < 0.06). However, flavonoid content was not significantly correlated with antioxidant activity in vegetables. It was observed that total phenolic content is the major contributor to the antioxidant activity of vegetables.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12712

scholarly journals ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY OF MELINJO (GNETUM GNEMON L.) SEED EXTRACTS AND MOLECULAR DOCKING OF ITS STILBENE CONSTITUENTS

2017 ◽  
Vol 10 (3) ◽  
pp. 243
Author(s):  
Abdul Mun'im ◽  
Muhammad Ashar Munadhil ◽  
Nuraini Puspitasari ◽  
Azminah . ◽  
Arry Yanuar
Keyword(s):  
Molecular Docking ◽  
Angiotensin Converting Enzyme ◽  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Converting Enzyme ◽  
Gnetum Gnemon ◽  
Ace Inhibitory

ABSTRACTObjectives: To evaluate the angiotensin converting enzyme (ACE) inhibitory activity of melinjo (Gnetum gnemon) seed extract and to study moleculardocking of stilbene contained in melinjo seeds.Methods: Melinjo seed powders were extracted with n-hexane, dichloromethane, ethyl acetate, methanol, and water successively. The extracts wereevaluated ACE inhibitory activities using ACE kit-Wist and the phenolic content using Folin–Ciocalteu method. The extract demonstrated the highestACE inhibitory activity was subjected to liquid chromatography-mass spectrometry (LC-MS) to know its stilbene constituent. The stilbene constituentsin melinjo seed were performed molecular docking using AutoDock Vina, and ligand-receptor Interactions were processed using Ligand Scout.Results: The ethyl acetate extract demonstrated the highest ACE inhibition activity with inhibitory concentration 50% value of 9.77 × 10−8 μg/mLand the highest total phenolic content (575.9 mg gallic acid equivalent/g). Ultra-performance LC-MS analysis of ethyl acetate extract has detected theexistency of resveratrol, gnetin C, ε-viniferin, and gnemonoside A/B. These compounds displayed similar physiochemical properties to lisinopril (ACEinhibitor), as in silico molecular docking studies demonstrated that they fit into the lisinopril receptors.Conclusion: In vitro analysis ethyl acetate extract from melinjo seeds demonstrated the highest ACE inhibitory activity. Molecular docking analysisindicated that resveratrol dimers, gnetin C and gnemonoside A can be considered ACE inhibitor.Keywords: Angiotensin converting enzyme inhibitor, Gnetum gnemon, Melinjo, Total phenolic, Antihypertension, Molecular docking.

scholarly journals Effects of Food Processing on Resveratrol and Total Phenolic Content in Melinjo (Gnetum gnemon L.) Seeds

2018 ◽  
Vol 10 (6) ◽  
pp. 1096-1100
Author(s):  
Islamudin Ahmad ◽  
Desta Andriyani ◽  
Christoper Gunawan ◽  
Nisrina Dhiah Fauziah ◽  
Sutriyo Sutriyo ◽  
...  
Keyword(s):  
Food Processing ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Gnetum Gnemon

Novel determination of the total phenolic content in crude plant extracts by the use of 1H NMR of the –OH spectral region

2011 ◽  
Vol 688 (1) ◽  
pp. 54-60 ◽  
Author(s):  
A.A. Nerantzaki ◽  
C.G. Tsiafoulis ◽  
P. Charisiadis ◽  
V.G. Kontogianni ◽  
I.P. Gerothanassis
Keyword(s):  
1H Nmr ◽  
Plant Extracts ◽  
Spectral Region ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic

Determination of optimal extraction conditions for phenolic compounds from Pistacia atlantica leaves using the response surface methodology

2016 ◽  
Vol 8 (31) ◽  
pp. 6107-6114 ◽  
Author(s):  
Ziyad Ben Ahmed ◽  
Mohamed Yousfi ◽  
Johan Viaene ◽  
Bieke Dejaegher ◽  
Kristiaan Demeyer ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Box Behnken Design ◽  
Pistacia Atlantica

Response surface methodology in combination with a Box–Behnken design was performed to optimize the extraction conditions, resulting in a maximum total phenolic content yield from Pistacia atlantica leaves.

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