scholarly journals STUDY OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE ENZYME AND ISOPROSTANE ON PREECLAMPSIA WITH NIFEDIPINE, METHYLDOPA, AND MAGNESIUM SULFATE THERAPY

2019 ◽  
pp. 163-166
Author(s):  
LINA LUKITASARI ◽  
ADITIAWARMAN ADITIAWARMAN ◽  
MASYHUDI MASYHUDI ◽  
SITI KHAERUNNISA ◽  
SUHARTATI SUHARTATI
Keyword(s):  
Enzyme Activity ◽  
Magnesium Sulfate ◽  
Drug Target ◽  
Renal Damage ◽  
Cross Sectional Study ◽  
Elisa Method ◽  
Cross Sectional ◽  
Dehydrogenase Enzyme ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Spearman’S Correlation

Objective: The objective of this research is to measure erythrocyte glucose-6-phosphate dehydrogenase (G6PD) enzyme activity and isoprostane andto correlate enzyme activity of G6PD with proteinuria and isoprostane in pregnant with proteinuria after the administration of nifedipine, methyldopa,and magnesium sulfate.Methods: This cross-sectional study was held in Soewandi Hospital, Surabaya, East Java, Indonesia. This study used total sampling as much as 30pregnant women with proteinuria who got nifedipine, methyldopa, and magnesium sulfate administration, age ranged from 17 to 48 years during theirthird trimester (>20 weeks). G6PD enzyme activity was measured from plasma by spectrophotometric method; plasma isoprostane was measuredby competitive-ELISA method; and proteinuria urine spot was analyzed by urine dipstick from standardized laboratory of the hospital. Statisticalanalysis used in this study was Spearman’s correlation coefficient.Results: In this research, the effect of proteinuria +1 (OR=0.056) is lower than proteinuria +3 level on the presence of high G6PD enzyme activity,and proteinuria +2 (OR=0.933) is lower than proteinuria +3 level on the presence of high G6PD enzyme activity in pregnant women with proteinuria.G6PD enzyme was positively correlated (p=0.08) with proteinuria, and the connection was statistically significant. There was no significant statisticcorrelation between G6PD enzyme activity and isoprostane concentration (p=0.797).Conclusion: This study found correlations between the enzyme activity of G6PD and proteinuria as the marker of renal damage in pre-eclampsia (PE)with the administration of nifedipine, methyldopa, and magnesium sulfate. However, it had no correlation with isoprostane as the marker of oxidativestress. This study suggests that there should be a concern about understanding the pathophysiology of proteinuria for possibility of drug target forindividuals with PE.

scholarly journals G6PD deficiency among malaria-infected national groups at the western part of Myanmar with implications for primaquine use in malaria elimination

2021 ◽  
Vol 49 (1) ◽  
Author(s):  
Kay Thwe Han ◽  
Zay Yar Han ◽  
Kyin Hla Aye ◽  
Khin Thet Wai ◽  
Aung Thi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Malaria Elimination ◽  
G6pd Deficiency ◽  
Control Program ◽  
Cross Sectional Study ◽  
Diagnostic Tools ◽  
Asymptomatic Malaria ◽  
Radical Cure ◽  
Readiness Assessment ◽  
Cross Sectional

Abstract Background Glucose 6-phosphate dehydrogenase deficiency (G6PDd) plays a central role in readiness assessment for malaria elimination in Myanmar by 2030 that includes primaquine (PQ) use. The risk of hemolysis in G6PDd individuals hampers the widespread use of primaquine safely in malaria-infected patients. In the pre-elimination era, it is important to screen initially for asymptomatic malaria in combination with G6PD deficiency by applying more sensitive diagnostic tools. Therefore, this study examined the proportion of G6PDd and the distribution of G6PD genotypes among malaria-infected national groups in Myanmar before initiation of malaria elimination strategies. Methods A cross-sectional study in one township each with high malaria burden from two states in the western part of Myanmar, was conducted during 2016-2018, and 320 participants (164 Rakhine and 156 Chin National groups) were recruited. We used RDT and ultrasensitive polymerase chain reaction (us PCR) method to confirm malaria infection, and a G6PD RDT(CareStart) to detect G6PDd and PCR/restriction fragment length polymorphism (RFLP) method to confirm the variant of G6PDd for genotyping. G6PD enzyme activity was measured by G6PD Biosensor (CareStart). Results Malaria positivity rates detected by RDT were lower than those detected by us PCR in the combined samples [13% (42/320) vs. 21% (67/320)] as well as in the Rakhine samples [17% (28/164) vs. 25% (41/164)] and in Chin samples [9% (14/156) vs. 17% (26/156)]. G6PD deficiency rates were approximately 10% in both the combined samples and specific national groups. For G6PD enzyme activity in the combined samples, G6PDd (defined as < 30% of adjusted male median) was 10% (31/320) and severe G6PDd (< 10% of AMM) was 3% (9/320). Among malaria-infected patients with positive by both RDT and usPCR, G6PDd was less than 20% in each national group. G6PD genotyping showed that the G6PD Mahidol (G487A) was the major variant. Conclusions The varying degree of G6PDd detected among malaria-infected national groups by advanced diagnostic tools, strongly support the recommend G6PD testing by the National Malaria Control Program and the subsequent safe treatment of P. vivax by primaquine for radical cure. Establishing a field monitoring system to achieve timely malaria elimination is mandatory to observe the safety of patients after PQ treatment.

scholarly journals Prevalence and quality of life in high school pupils with acne in Serbia

2013 ◽  
Vol 70 (10) ◽  
pp. 935-939 ◽  
Author(s):  
Jelena Peric ◽  
Natasa Maksimovic ◽  
Janko Jankovic ◽  
Biljana Mijovic ◽  
Vesna Reljic ◽  
...  
Keyword(s):  
Quality Of Life ◽  
High School ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Demographic Questionnaire ◽  
Study Population ◽  
Serbian Version ◽  
The Impact ◽  
Spearman’S Correlation

Background/Aim. Acne is a common problem in adolescent children with considerable emotional and psychological effects. The aim of this study was to determine the self-reported prevalence of acne and to assess its impact on the quality of life in high school pupils in Serbia. Methods. The cross-sectional study was conducted in May 2011 in two medical high schools in Serbia. Only pupils who gave a written informed consent to participate in the study (n = 440) were asked to fill in two questionnaires: short demographic questionnaire and Cardiff Acne Disability Index (CADI), a disease-specific questionnaire measuring disability induced by acne. Internal consistency (tested by Cronbach?s alpha) and item-total score correlations (Spearman's correlation analysis) were used for reliability analyses. Results. The study population consisted of 440 pupils, 281 from Belgrade and 159 from Uzice. Among them 371 (84.3%) were girls and 69 (15.7%) boys, with similar sex distribution in Belgrade and Uzice. The total mean age of pupils was 16.48 years (SD = 0.55). Out of 440 pupils 228 (51.8%) self-reported their acne. The acne prevalence was significantly higher in pupils from Uzice (73.6%) than in those from Belgrade (39.6%). The overall mean CADI score for the whole sample was 2.87 ? 2.74, with the similar quality of life impairment in adolescents from Belgrade and from Uzice. The mean Cronbach?s alpha was 0.82. Conclusion. This study shows that the quality of life impairment due to acne is mild for the majority of the affected pupils. The Serbian version of the CADI is a reliable, valid, and valuable tool for assessing the impact of acne on the quality of life.

scholarly journals Increased Interleukin-6 as Infl ammatory Response and Magnesium Defi ciency in Pre-dialysis Chronic Kidney Disease of Indonesian Children

2021 ◽  
Vol 9 (2) ◽  
pp. 93
Author(s):  
Astrid Kristina Kardani ◽  
Ninik Asmaningsih Soemyarso ◽  
Jusli Aras Aras ◽  
Risky Vitria Prasetyo ◽  
Mohammad Sjaifullah Noer
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Interleukin 6 ◽  
Inverse Correlation ◽  
Cross Sectional Study ◽  
The Body ◽  
Cross Sectional ◽  
Duration Of Illness ◽  
History Of ◽  
Spearman’S Correlation

Chronic kidney disease (CKD) is a serious health problem in children, with increasing morbidity and mortality rates throughout the world. Children with CKD tend to experience magnesium (Mg) defi ciency that can stimulate an infl ammatory response in the body. One of the infl ammatory responses is an increase of Interleukin-6 (IL-6).  Study to analyze the correlation between Mg and IL-6 in pre-dialysis CKD children. The methods a cross sectional study was conducted in Dr Soetomo General Academic Hospital from November 2018 to April 2019. Children with pre-dialyis CKD were included in this study. Variables of serum Mg level (mg/dL) and infl ammatory marker (IL-6) were measured from the blood and analyzed by ELISA method. The correlation between Mg and IL-6 was analyzed with Spearman’s correlation test with p <0.05.  Result a total of 47 children (27 boys vs 20 girls) between 3 months to 18 years old, with pre-dialysis CKD and no history of magnesium supplementation were included. The primary disease that causes of CKD were lupus nephritis (38.3%), nephrotic syndrome (23.4%), urologic disorder (23.4%),  tubulopathy (10.6%) and others (4.3%). The average IL-6 level was 55.42±43.04 pg/dL and Mg level was 2.06±1.54 mg/dL. There were no signifi cant correlation between IL-6 level and Mg level with staging of CKD and duration of illness (p>0.05), but there was a signifi cant correlation between serum Mg level and IL-6 level (r=-0.748; p<0.001). Magnesium levels have a signifi cant inverse correlation with IL-6 levels in pre-dialysis CKD children. The lower the Mg levels in the blood, the higher IL-6 levels and vice versa. 

scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
pp. 305-309
Author(s):  
AFRIWARDI AFRIWARDI ◽  
DELMI SULASTRI ◽  
YUNIAR LESTARI ◽  
DESMAWATI DESMAWATI ◽  
PRIMA MINERVA
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Telomere Length ◽  
Saturated Fatty Acids ◽  
Cross Sectional Study ◽  
Fat Intake ◽  
Sod Activity ◽  
Cross Sectional ◽  
Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia. Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity. Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037). Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

scholarly journals Effect of caregiver characteristics on dementia management strategies

2016 ◽  
Vol 10 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Débora Dalpai ◽  
Ramon Castro Reis ◽  
Analuiza Camozzato de Pádua
Keyword(s):  
Caregiver Burden ◽  
Management Strategies ◽  
Cross Sectional Study ◽  
Porto Alegre ◽  
Cross Sectional ◽  
Bivariate Correlation ◽  
Gender Education ◽  
Correlation Tests ◽  
Spearman’S Correlation ◽  
Moderate Positive Correlation

ABSTRACT Caregiving has an important influence on the prognosis of dementia, particularly regarding the management strategy implemented. Therefore, evaluating which characteristics of caregivers can influence the choice of a particular strategy for managing dementia is needed. Objective: To evaluate the association between characteristics of caregivers and their management strategies as applied to patients with dementia. Methods: A cross-sectional study involving 45 professional caregivers from two nursing homes in Porto Alegre, Brazil, was conducted. Age, gender, education, years as a caregiver, income, burden, depressive and anxiety symptoms and dementia management strategies were evaluated for all participants. Pearson's or Spearman's correlation tests were applied according to the variable distribution (parametric or non-parametric). Bivariate correlation analysis was applied. P<0.05 was considered statistically significant. Results: There was a significant and moderate positive correlation between burden measured by the Zarit Burden Interview and criticism measured by the Dementia Management Strategies Scale (Spearman's rho = 0.555, p < 0.001). No other correlations were observed. Conclusion: Among the caregiver characteristics that directly affect the approach to managing dementia, high caregiver burden was found to be associated with high criticism, an authoritative way of managing dementia. This exploratory study indicated that a possible way of decreasing negative dementia management is to reduce caregiver burden.

scholarly journals Seroprevalence of Malaria and Hepatitis B Coinfection among Pregnant Women in Tamale Metropolis of Ghana: A Cross-Sectional Study

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Gideon Kofi Helegbe ◽  
Paul Armah Aryee ◽  
Baba Sulemana Mohammed ◽  
Anthony Wemakor ◽  
David Kolbila ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Hiv Infection ◽  
Pregnant Women ◽  
Cross Sectional Study ◽  
Hbv Infection ◽  
Full Blood Count ◽  
Cross Sectional ◽  
Tamale Metropolis ◽  
The Mean ◽  
Glucose 6 Phosphate Dehydrogenase

Background. Coinfections are becoming common risk factors that may contribute to the increased burden of morbidity in pregnancy. The aim of this study was to assess the seroprevalence of coinfections of malaria, hepatitis B (HBV), human immunodeficiency virus (HIV), and syphilis among pregnant women attending antenatal clinics (ANC) in the Tamale Metropolis. Methods. By means of rapid diagnostic tests (RDTs), pregnant women attending the Tamale Teaching Hospital (TTH) were screened for malaria, HBV infection, HIV infection, and syphilis from March 2013 to February 2015. Haemoglobin (Hb) values, sickling, and glucose-6-phosphate dehydrogenase deficiency (G6PDd) statuses were also assessed using full blood count (FBC), sodium metabisulphite, and methaemoglobin reduction tests, respectively. Logistic regression analysis was performed to estimate the risks/odds ratios (ORs) for the coinfections and other variables (age, gravidity, and time of the first ANC visit) with 95% confidence intervals (CIs) and set p values for accepting any differences at <0.05. Results. Within the two-year study period, data were collected from 3,127 pregnant women. The mean age (SD) of the pregnant women was 28.5 (±5.0) years. Of the total number, seroprevalence was high for malaria (11.6%) and HBV infection (4.2%) and low for HIV infection (1.0%) and syphilis (0.4%) monoinfections. Mal/HBV coinfection was higher (0.7%) when compared with Mal/HIV (0.1%), Mal/syphilis (0.0%), HBV/HIV (0.0%), HBV/syphilis (0.1%), and HIV/syphilis (0.0%) coinfections. The mean Hb (g/dl) for the women with the four monoinfections was significantly different from one another (p=0.009). Pregnant women with malaria infection were about 2 times more likely to be coinfected with HBV even after adjusting for potential confounders (adjusted odds ratio (AOR) = 1.66, 95% CI = 1.04–2.65, p=0.031). Those in their third trimester and visiting the ANC for the first time were significantly less likely to be infected with HBV (AOR = 0.45, 95% CI = 0.28–0.73, p=0.001), with malaria/HBV coinfection (AOR = 0.09, 95% CI = 0.01–0.68, p=0.020), and with any coinfection (AOR = 0.19, 95% CI = 0.06–0.63, p=0.007). Conclusion. A comparatively high seroprevalence of malaria and its coinfection with HBV in pregnant women was observed in this study. Considering the effects that both malaria and HBV have on the liver, it would be expedient to conduct further studies to assess liver function among malaria/HBV-infected individuals, while interventions to prevent coinfections among pregnant women are intensified.

Omentin-1 and diabetic foot

2022 ◽  
pp. 153473462110698
Author(s):  
Tsvetan Gatev ◽  
Viktoriya Byalkova ◽  
Ivan Poromanski ◽  
Tsvetelina Velikova ◽  
Dobrin Vassilev ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Metabolic Disorders ◽  
Bioelectrical Impedance ◽  
Cross Sectional Study ◽  
Limited Data ◽  
Elisa Method ◽  
Cross Sectional ◽  
Diabetic Foot Disease ◽  
Foot Disease

Introduction: Diabetic foot disease is an advanced complication of diabetes mellitus, which is associated with severe invalidization and high mortality rate among affected people. Many factors are involved in its pathogenesis but not all of them are fully elucidated. Objectives: Adipose tissue and its hormones – adipokines, are related to diabetic complications and metabolic disorders. Until now, there are limited data on their role in diabetic foot. The aim of this cross-sectional study is to determine the levels of the adipokine omentin-1 in people with and without diabetic foot disease and to look for its potential involvement in this complication. Methods: Eighty patients with type 2 diabetes and mean age of 60.8±10.5 years were included in this study. They were divided into two groups: with (n=36) and without (n=44) diabetic foot disease. Standard antrometric, clinical and laboratory tests were made. Body composition was analyzed by bioelectrical impedance based device. Serum omentin-1 was measured using ELISA method. Results: Levels of omentin-1 were significantly higher among people with diabetic foot disease (700.2±345.1 ng/ml), compared to the other group (560.2±176.7 ng/ml). This difference remained significant even after adjusting for potential confounders. In a regression model omentin-1 proved its predictive value for development of diabetic foot. Conclusion: Adipokines, and particularly omentin-1, might be included in the pathogenesis of diabetic foot disease.

Prevalence of Eclampsia and its Maternal-fetal outcomes at Ghandi Memorial Hospital, Addis Ababa Ethiopia, 2019. Retrospective study

2020 ◽  
Author(s):  
Addisu Yeshambel ◽  
Walellign Anmut
Keyword(s):  
Magnesium Sulfate ◽  
Addis Ababa ◽  
Fetal Outcome ◽  
Cross Sectional Study ◽  
Adverse Outcomes ◽  
Morbidity And Mortality ◽  
Memorial Hospital ◽  
Fetal Outcomes ◽  
Cross Sectional ◽  
Sub Saharan

Abstract Background: Eclamptic disorder of pregnancy is one of the common problems in sub-Saharan countries and forms one of the deadly triads along with hemorrhage and infection which complicates maternal and fetal outcomes of pregnancy. To assess the prevalence of eclampsia and its maternal and fetal outcome in Ghandi Memorial Hospital, Addis Ababa Ethiopia, 2019.Methods: A descriptive retrospective cross-sectional study was used on randomly selected 185 women who attended delivery at Ghandi memorial Hospital from 1st of September 2017 to –last of August 2018. Data were analyzed using SPSS version 25 software. Descriptive statistics were used to calculate frequencies and percentages and data was presented using texts and, tables. Results: Out of the 2,973 deliveries, the prevalence of eclampsia was found to be 16.1%. About 89.7% had reported a history of prior pregnancy-induced hypertension and 73.5% induced their current pregnancy following eclampsia. From mothers required interventions to terminate the pregnancy by induction, 47.8% ended by cesarean section secondary to non-reassuring fetal status (29.2%). The majority (91.9%) had taken magnesium sulfate for the management of convulsion and 86.5% had taken hydralazine for hypertension management. Abruption of the placenta (96.2%), postpartum-hemorrhage (89.2%), and HEELP syndrome (83.8 %) were major maternal adverse outcomes reported, and 33% of pregnancy was ended as stillbirth followed by low birth weight (28.6%). Over 53.6% of delivered babies had an APGAR score of less 4 and 30.4% of neonates required admission to nursery/NICU referral. Conclusion: The prevalence of eclampsia was high, with corresponding high maternal and perinatal morbidity and mortality. Increasing early detection before pregnancy, antenatal screening, and use of magnesium sulfate to control convulsions will reduce the disorder and associated morbidity and mortality for both mother and fetus.

scholarly journals Prevalence of Urinary Incontinence in School Going Children: A Cross-sectional Study

2021 ◽  
Vol 18 (4) ◽  
pp. 676-680
Author(s):  
Nistha Shrestha ◽  
Salina Sahukhala ◽  
Diva K.C ◽  
Dawn Sandalcidi ◽  
Shambhu Prasad Adhikari
Keyword(s):  
Urinary Incontinence ◽  
Descriptive Analysis ◽  
Psychological Impact ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Keywords Children ◽  
Symptom Index ◽  
Cross Sectional ◽  
Medical Practitioners ◽  
Spearman’S Correlation

Background: Urinary incontinence is a highly prevalent condition that affects both gender across the age span and has significant social and psychological impact. The objective of this study was to determine the prevalence of urinary incontinence in school going children and assess the association of Incontinence Symptom Index-Pediatrics with age and gender.Methods: A cross-sectional study was carried among 305 children aged 11-16 years using convenient sampling in a school of Dhulikhel municipality. Data were analysed using descriptive analysis and spearman’s correlation coefficient.Results: Prevalence of stress, urge and nocturnal enuresis were 22.95%, 19.34% and 3.93% respectively. Gender was significant with stress (p>0.001) and urge (p>0.039) incontinence whereas age was significant with nocturnal enuresis.Conclusion: The episode of Urinary incontinence is common in school going children. It’s important that children, parents, teachers and medical practitioners be aware of these phenomena. Keywords: Children; prevalence; urinary incontinence

scholarly journals HUBUNGAN LAMANYA PEMBERIAN MAGNESIUM SULFATE PADA PASIEN PEB DAN EKLAMPSIA DENGAN KEJADIAN HIPERMAGNESIUM DI RSUP DR. M. DJAMIL PADANG

2018 ◽  
Vol 2 (1) ◽  
pp. 21-25
Author(s):  
Mayuliani Mayuliani ◽  
Dovy Djanas
Keyword(s):  
Pregnant Women ◽  
Magnesium Sulfate ◽  
Cross Sectional Study ◽  
Research Data ◽  
Severe Preeclampsia ◽  
Sectional Study ◽  
Inclusion Criteria ◽  
Chi Square ◽  
Cross Sectional ◽  
The Relationship

Objective :To find out the relationship between the duration of MgS04 administration in severe preeclampsia patients and eclampsia with the incidence of hypermagnesium.Method :This study is a comparative observational study using a cross sectional study design. The study was conducted in the medical record of Dr.M.Djamil Padang Hospital during the period January 1, 2016 to August 31, 2017 of pregnant women with severe preeclampsia and eclampsia, found 30 study subjects who met the inclusion criteria. Furthermore, data is recorded in a research form that has been provided, then carried out research data processing. Statistical analysis to assess significance using the chi-square test.Results : Magnesium levels increased in pregnant women with severe preeclampsia and eclampsia by 21 correspondents. Increased Magnesium levels in patients with severe preeclampsia and eclampsia who received MgS04 treatment for 48 hours by 16 correspondents (84.2%) and 5 correspondents with MgS04 administration for 24 hours.Conclusion : There is a significant relationship between the duration of Magnesium Sulfate with the incidence of hyperpermagnesium in patients with severe preeclampsia and eclampsia. Keywords: severe preeclampsia, eclampsia, magnesium sulfate, magnesium levels

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