scholarly journals ANTI-HYPERGLYCEMIC EFFECT OF TERMINALIA CATAPPA FRUIT EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 9 (4) ◽  
pp. 212 ◽  
Author(s):  
Tapan Behl ◽  
Anita Kotwani
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Urine Volume ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Dependent Manner ◽  
Fruit Extract ◽  
Terminalia Catappa ◽  
Hyperglycemic Effect

Objective: To explore the anti-hyperglycemic effect of fruit extract of Terminalia catappa (Indian almond), a potential medicine from plant origin in a diabetic rat model.Methods: Streptozotocin-induced chronic diabetic rat model was utilized in the study. Three doses of test drug, hydro-alcoholic fruit extract of Terminalia catappa in 20 mg/kg, 30 mg/kg and 40 mg/kg and a standard anti-diabetic drug, glibenclamide (10 mg/kg) was used. The study had a total of nine groups with eight animals in each group. Drugs were given orally every day for 12 w. Blood glucose, body weight and urine volume were measured weekly, glycosylated hemoglobin (HbA1c) was estimated at 12th week in all groups. Data for all parameters were analyzed using one-way ANOVA repeated measures followed by Mann-Whitney test.Results: Hydro-alcoholic fruit extract of T. catappa significantly decreased blood glucose, urine volume and increased body weight in a dose-dependent manner in diabetic rats. At 12th week, blood glucose level in control, diabetic control, glibenclamide, T. catappa (40 mg/kg) group was 96.25±2.05 mg/dl, 599.75±0.25 mg/dl, 248.25±11.45 mg/dl, 115.00±3.78 mg/dl respectively. Effect of T. catappa in 30 mg/kg and 40 mg/kg dose was significantly more than glibenclamide. At 12th week, HbA1c level in control, diabetic control, glibenclamide, T. catappa (40 mg/kg) was 2.94±0.33 mmol/l, 4.94±0.49 mmol/l, 3.61±0.28 mmol/l, 3.21±0.27 mmol/l. Treatment with T. catappa 30 mg/kg, 40 mg/kg and glibenclamide brought back the level of HbA1c to normal levels. The addition of glibenclamide to T. catappa (40 mg/kg) did not produce any additional effect on blood glucose and HbA1c levels compared to the effect of T. catappa (40 mg/kg) in diabetic rats.Conclusion: Terminalia catappa fruit extract exhibited a significant anti-hyperglycemic effect in diabetic rats and has a great potential to be used in diabetes.

Terminalia catappa fruit extract reverses streptozotocin- induced diabetic retinopathy in rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Tapan Behl ◽  
Thirumurthy Velpandian ◽  
Anita Kotwani
Keyword(s):  
Diabetic Retinopathy ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Inflammatory Biomarkers ◽  
Repeated Measure ◽  
Diabetic Rat ◽  
Dependent Manner ◽  
Fruit Extract ◽  
Terminalia Catappa ◽  
Anti Inflammatory

Objective and background: Diabetic retinopathy is amongst the most common microvascular complication associated with diabetes. Controlling blood glucose level alone cannot manage diabetes associated complications. Thus, mechanism that additionally prevent diabetes associated complication are need of the hour, driving the researchers towards herbal therapies. Terminalia catappa is renowned for its anti-inflammatory, antioxidant, anti-hyperglycemic and anti-angiogenic activity. The current study explores the effect of Terminalia catappa fruit extract in streptozotocin-induced diabetic retinopathy in rats. Methods: Streptozotocin-induced chronic diabetic rat model was utilized in the study. Hydro-alcoholic fruit extract of T. catappa in 20mg/kg, 30mg/kg and 40mg/kg dose and standard anti-diabetic drug, glibenclamide (10mg/kg) was given orally. Retinopathy was evaluated by monitoring lenticular, fundus images and measuring arteriole and venule tortuosity index. Oxidative, angiogenic and inflammatory biomarkers were assessed at 12th week in retinal homogenate. Histopathological changes in retina were also examined. Data was analyzed using one-way Repeated Measure ANOVA followed by MannWhitney test. Results: Hydro-alcoholic fruit extract of T. catappa significantly decreased blood glucose (p<0.001) in dose-dependent manner in diabetic rats. Cataract lens was observed in all experimental groups and became clear (grade 0) with 40mg/kg and with 40mg/kg along with glibenclamide at eight and sixth week respectively. Hydro-alcoholic fruit extract in all three doses significantly reduced (p<0.01) arteriole and venule tortuosity in diabetic rats. T. catappa in all three doses in diabetic rats showed modulatory effect in oxidative, angiogenic and inflammatory biomarkers. Conclusion: T. catappa reverses diabetes-induced retinopathy by anti-hyperglycemic, anti-oxidant, anti-angiogenic and anti-inflammatory actions, and thus has a potential to be used in diabetes-induced retinopathy.

scholarly journals BANANA PEEL FLAKES ALLEVIATE BLOOD GLUCOSE AND STRESS IN A DOSE-DEPENDENT MANNER

2020 ◽  
pp. 75-81
Author(s):  
ANDREANYTA MELIALA ◽  
YUSTINA ANDWI ARI SUMIWI ◽  
PARAMITA NARWIDINA ◽  
SRI LESTARI SULISTYO RINI ◽  
WIDIASTUTI SETYANINGSIH
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Banana Peel ◽  
Standard Diet ◽  
Dependent Manner ◽  
Immobility Time ◽  
Healthy Control

Objective: This study aimed to evaluate the antidiabetic and antidepressant effects of banana peel flakes in streptozotocin-induced diabetic rats. Methods: Twenty-five male Wistar rats were classified into five groups with different treatments. Groups I to IV were diabetic rats model groups that consumed only standard diet, standard diet containing 5%, 10%, and 20% of banana peel flakes, respectively. While group V was a healthy control group fed a standard diet. Immunohistochemistry staining was measured to examine serotonin expression in the colon and pancreas. Results: The diabetic rats treated with 20% banana peel flakes had a lower blood glucose concentration (p<0.05) compared with diabetic control and showed a shorter duration of immobility time (p<0.05) than the healthy control. Additionally, compared with diabetic control, the diabetic rats treated with 5% banana peel flakes showed higher serotonin expression (p<0.05) in the colon. In contrast, serotonin expression in the pancreas did not show any significant difference (p>0.05). Conclusion: The present study disclosed that the banana peel flakes provided an antidepressant effect in the diabetic rats model, which might occur through the mechanism of controlling blood glucose concentration.

scholarly journals Antidiabetic potential of the leaf extract of Centella asiatica in alloxaninduced diabetic rats

2016 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Talha Bin Emran ◽  
Mycal Dutta ◽  
Mir Muhammad Nasir Uddin ◽  
Aninda Kumar Nath ◽  
Md Zia Uddin
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat

The study was designed to evaluate the glucose and cholesterol lowering effect of the aqueous extract of Centella asiatica leaf using the alloxan-induced diabetic rats and compared the activity with diabetic control and antidiabetic drug (Glibenclamide). Leaf extract (50 mg/kg) of C. asiatica and Glibenclamide were administered to normal and experimental diabetic rats for the duration of 10 days. In the alloxan-induced diabetic rat model, C. asiatica extract (50 mg/kg) significantly (p < 0.05) lowered the fasting blood glucose level as well as the total cholesterol level. Serum insulin levels were not stimulated in the animals treated with the extract. In addition, changes in body weight, serum lipid profiles and liver glycogen levels assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. Significant results (p < 0.05) were observed in the estimated parameters. Surprisingly, body weight was increased significantly (p < 0.05) in the C. asiatica treated diabetic group. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, steroids and tannins in significant amountsJahangirnagar University J. Biol. Sci. 4(1): 51-59, 2015 (June)

scholarly journals Ameliorative Potentials of Cocoyam (Colocasia esculentaL.) and Unripe Plantain (Musa paradisiacaL.) on the Relative Tissue Weights of Streptozotocin-Induced Diabetic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
C. O. Eleazu ◽  
M. Iroaganachi ◽  
K. C. Eleazu
Keyword(s):  
Body Weight ◽  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
Chemical Composition ◽  
Growth Rates ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Musa Paradisiaca ◽  
Urine Assay ◽  
Standard Techniques

Aim. To investigate the ameliorating potentials of cocoyam (Colocasia esculentaL.) and unripe plantain (Musa paradisiacaL.) incorporated feeds on the renal and liver growths of diabetic rats, induced with 55 and 65 mg/kg body weight of Streptozotocin.Method. The blood glucose level of the rats was measured with a glucometer, the protein and glucose and specific gravity (SPGR) in the urine samples of the rats were measured using urine assay strips and urinometer respectively. The chemical composition and antioxidant screening of the test feeds were carried out using standard techniques.Results. Administration of the test feeds for 21 days to the diabetic rats of groups 4 and 5, resulted in 58.75% and 38.13% decreases in hyperglycemia and amelioration of their elevated urinary protein, glucose, SPGR, and relative kidney weights. The diabetic rats administered cocoyam incorporated feeds, had 2.71% and 19.52% increases in weight and growth rates, the diabetic rats administered unripe plantain incorporated feeds had 5.12% and 29.52% decreases in weight and growth rates while the diabetic control rats had 28.69%, 29.46%, 248.9% and 250.14% decreases in weights and growth rates. The cocoyam incorporated feeds contained higher antioxidants, minerals and phytochemicals except alkaloids than unripe plantain feed.Conclusion. Cocoyam and unripe plantain could be useful in the management of diabetic nephropathy.

scholarly journals Hypoglycaemic and Hypolipidemic Effects of Treculia africana Aqueous Leaves Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
Idris A. Kankara ◽  
Gayus A. Paulina ◽  
M. Aliyu
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Treculia Africana

This study investigated the hypoglycaemic and hypolipidaemic effects of Treculia africana plant used in Nigeria as medicinal plant. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Twenty five (25) male albino rats were divided into five groups, five (5) rats per group; normal control, diabetic control and diabetic groups treated with aqueous leaves extract of 200,400 and 800 mg/Kg body weight respectively for 21 days orally. The effects of the extract on some biochemical parameters were evaluated; fasting blood glucose level was assayed using glucose oxidase method, total cholesterol and HDL –cholesterol were assayed using enzymatic method while LDL- cholesterol was determined by Friedewald equation. The results showed that, extract significantly (p<0.05) decrease the elevated fasting blood glucose levels, total cholesterol, triglyceride and LDL- cholesterol when compared with the diabetic control rats. The extract also caused significant (p<0.05) increased in HDL –cholesterol and body weight when compared with diabetic control rats. Aqueous leave extract of Treculia africana possess hypoglycemic effect and the most effective dose was 800 mg/Kg body weight in amelioration of hyperglycaemia and most all toxicity effects of alloxan on lipid profile.

scholarly journals Anti-hyperglycemic Effects of Diacerein in Alloxan-Induced Diabetes Mellitus in Wistar Albino Rats

2020 ◽  
pp. 107-113
Author(s):  
Arsalan Uqaili ◽  
Samia Siddiqui ◽  
Roomi Aijaz ◽  
Yar Muhammad Nizammani ◽  
Navaid Kazi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Group B ◽  
Group D

Objective: To determine the anti-hyperglycemic effects of interleukin-1 inhibitor (diacerein) in alloxan induced diabetic albino wistar rats. This experimental study was performed at the Department of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam within 6 months from April 2016 to September 2016. Total of 160 adult Albino Wistar Rats having an average of 200 to 300 grams body weights were selected. Animals were categorized into 4 groups as; Group A (n=15): Control rats – receive 0.9% normal saline as placebo Experimental Groups Group B (n=15): Experimental Control (Diabetic rats) - Alloxan50 mg/kg body weight intraperitoneal. Group C (n=15): Diabetic rats + Diacerein (30 mg/kg/day) orally daily. Group D (n=15): Diabetic rats + Diacerein (50 mg/kg/day) orally daily. Animals were kept and treated as per the NIH Guideline for Use and Care of Laboratory Animals. Diabetes mellitus was induced via a single intraperitoneal injection of 50 milligram/kg alloxan monohydrated dissolved in aseptic 0.9% saline. After 72 hours, blood specimens were taken from the caudal vein of the rats and glucose level>200 mg/dL was taken as diabetes. Experimental rats were given diacerein approximately 30 and 50 mg orally for 6 weeks. At the completion of experiment the body weight was measured of each animal by electronic measuring balance and blood sample was taken from each animal of all groups to assess the blood glucose level and HbA1c level. Data were recorded via self-made proforma and analysis was done by using SPSS version 20. Results: Average body weight of Diabetic control (Group B) was 193.33±22.50 grams, which was lower in contrast to Diacerein treated group C 202.47±25.70 grams and significantly lower as compared to Diacerein treated group D as  212.6±23.43 grams. A significant increase in blood glucose levels 182.07±10.63 mg/dl was noted in the Diabetic control (Group B) compared to Diacerein treated group C (110.13± 8.54 mg/dl) and group D (85.87±8.41 mg/dl) (P=0.001). HbA1c was markedly raised in the Group B- diabetic controls, while diacerein treated diabetic rats (groups C and D) showed a significant decrease in HbA1c (P=0.001). Conclusion: It was concluded that Diacerein achieves the Euglycemic state by reducing the levels of blood glucose and glycated hemoglobin (HbA1c) in Alloxan-Induced diabetes mellitus in Wistar Albino Rats.

scholarly journals Antidiabetic activity of curry leaves Murraya koenigii on glucose level, kidney and islets of langerhans in streptozotocin induced diabetes in rats

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Imad M. Al-Ani ◽  
Rahajoe I. Santosa ◽  
Muhammad H. Yankuzo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Murraya Koenigii ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Curry Leaves ◽  
Aqueous Leaf Extract

Introduction: This study examined the antihyperglycemic effect of curry leaves, Murraya koenigii “MK” aqueous extract, and to examine its possible protective effects on the Islets of Langerhans and kidneys in streptozotocin (STZ) induced diabetic rats.  Methods: Thirty healthy adult male Sprague Dawley rats were randomized into five groups (n=6); normal control, normal treated with “MK” control, diabetic control (non-treated with “MK”), diabetic treated with 200mg/kg MK aqueous leaf extract and diabetic treated with 400mg/kg MK aqueous leaf extract. Blood glucose levels and body weight were monitored. The animals were sacrificed on the 30th day; the kidney and pancreatic tissues were processed for histological studies. Results: The diabetic control group significantly (p<0.001) showed considerable loss of body weight and increase in blood glucose levels and degeneration of the glomeruli and renal convoluted tubules and atrophied islets with disintegration of β-cells. Treatment of diabetic rats with aqueous extract showed significant (p<0.001) improvement in blood glucose levels and body weight gain.  The MK extract also caused an improvement in tissue injury induced by STZ injection in the kidney and endocrine pancreas.  Conclusions: These findings highlighted the beneficial effects of MK aqueous extract against cellular oxidative damage in STZ-induced diabetic rats.

scholarly journals Polygonatum sibiricum extract exerts inhibitory effect on diabetes in a rat model

2021 ◽  
Vol 18 (7) ◽  
pp. 1493-1497
Author(s):  
Lai-zeng Yu ◽  
Xue-peng Zhang ◽  
Ying-xin Wang
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Dependent Manner

Purpose: To investigate the effect of Polygonatum sibiricum extract (PSE) on streptozotocin-induced diabetic rats. Methods: PSE was obtained by steeping the dried Polygonatum sibiricum in water at 60 oC three times, each for 1 h, before first drying in an oven at 100C and then freeze-drying the final extract, thus obtained. Diabetic model rats were prepared by a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control, normal control, reference (glibenclamide1 mg/kg) as well as PSE groups, (35, 70 and 140 mg/kg). Blood glucose and plasma insulin levels were measured to determine antihyperglycemic effect. Oxidative stress was evaluated in liver and kidney by their antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats. Results: Compared with diabetic rats, oral administration of PSE at a concentration of 120 mg/kg daily for 30 days showed a significant decrease in fasting blood glucose (118.34 ± 3.29 mg/dL) (p < 0.05) and increased insulin level (12.86± 0.62 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.83 ±0.21 mg/dL, p < 0.05) and serum urea (43.26±1.42 mg/dL, p < 0.05). Conclusion: The results suggest that PSE may effectively normalize impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. Thus, PSE has a protective effect against lipid peroxidation by scavenging free radicals, restoration of insulin function, and reduction of the incidence of complications.

scholarly journals Protein Isolates from Fermented Melon Seeds Promoted Differential Expression in Liver and Pancreas of Streptozotocin Induced Diabetic Male Wistar Rats

2020 ◽  
pp. 6-18
Author(s):  
A. O. Iyoriobhe ◽  
A. O. Abiola ◽  
P. A. Adedigba ◽  
P. H. Fagbenro ◽  
T. A. Adebisi ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Protein Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Protein Isolates ◽  
Male Wistar Rats ◽  
Melon Seeds

Diabetes mellitus (DM) is the most common endocrine disorder of human. However, the anti-diabetic activity of protein isolates from fermented plants seed for DM remains enigmatic. The prevalence of diabetes in Africa is exponentially increasing with more deaths occurring directly from diabetes mellitus or from its associated complications. The current study investigated the effect of Protein isolate from fermented melon seeds (Ogiri; OPI) of Cucumeropsis manni on blood glucose, hepatic and pancreatic protein profile, histopathological parameters, identification and characterisation of expressed proteins in streptozotocin (STZ)-induced diabetic rats. Thirty Male wistar rats were divided into nondiabetic control, STZ-diabetic control, STZ-Ogiri protein isolate supplemented group (STZ-OPI; 200 mg/kg diet), STZ-Ogiri protein isolate supplemented group (STZ-OPI; 600 g/kg diet) and STZ-glibenclamide treated group (STZ-GBN; 0.5 mg/kg diet). Diabetes was induced by a single injection of STZ (60 mg/kg BW) freshly dissolved in 0.1 mol/L citrate buffer (pH 4.5) into the intraperitonium. Diabetes was confirmed by measuring the fasting blood glucose concentration 48-h post-injection. The rats with blood glucose level above 290 mg/dL were considered to be diabetic. Ogiri protein isolates was supplemented in the diet for 6 weeks. The supplementation OPI reduced (P< 0.05) the blood glucose concentration of the STZ-induced diabetic rats. OPI supplemented groups had significantly higher percentage body weight gain. The high dose OPI supplemented group had a lowest liver protein concentration (19.39 mg/dl) but a significantly (P< 0.05) higher pancreas protein concentration when compared to all the diabetic control. Histological sections of examined tissue revealed accumulation of fat in the liver of diabetic rats and necrosis of the islet of Langerhans were observed in the pancreas. 1DE SDS-PAGE of hepatic and pancreatic tissue homogenates revealed differential expression of 150 kDa proteins in rats treated with 200 mg/kg body weight of OPI only and 20-25 kDa proteins in rats treated with 600 mg/kg body weight of OPI respectively. This result show that OPI supplementation may impose a direct or indirect inhibitory or post translational modification on specific proteins implicated in hyperglycemia and diabetes and as such be a potential antihyperglycemic agent in the management of diabetes.

scholarly journals Allogeneic diabetic mesenchymal stem cells transplantation in streptozotocin-induced diabetic rat

2008 ◽  
Vol 31 (6) ◽  
pp. 328 ◽  
Author(s):  
Qing-Yu Dong ◽  
Li Chen ◽  
Guan-Qi Gao ◽  
Lei Wang ◽  
Jun Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Body Weight ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Pancreatic Regeneration ◽  
Insulin Producing Cells ◽  
The Status

Background: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stroma cells which can provide a potential therapy for diabetes mellitus. But the mechanism is still controversial. Also, the status of BM-MSCs under hyperglycemia is not known. In the present study, we investigated the status of BM-MSCs in experimental-diabetic rat and demonstrated the rescue of experimental diabetes by diabetic MSCs transplantation. Methods: BM-MSCs were cultured and the potential of multiple-differentiation was identified through induction into osteoblasts. MSCs of passage 3 were used for the following experiment. The MSCs were labeled with 5-bromo-2?-deoxyuridine (BrdU). Diabetes in rats was induced by STZ injection. The rats were divided into three groups: normal control group (no DM, rats treated with saline through tail vein, n=10); DM control group (DM, no transplantation of MSCs, n=20); experimental group (DM and transplantation of MSCs, n=20). Body weight and blood glucose of the rats were monitored during the experiment after transplantation of MSCs. Paraffin sections of pancreas were obtained from rats of each group. Immuno-histochemistry analysis and double immunofluorescence were used to detect the BM-MSCs in the pancreatic tissue and their differentiating state. Results: MSCs were 89.5% labeled by BrdU and DAPI, which was green/blue double stained under fluorescent microscopy. Transplantation of diabetic MSCs resulted in a reduction of hyperglycemia on day 45 in experimental diabetic rats compared with control rats (17.7 mM ±3.9 vs 27.8 mM ± 2.1, P < 0.05), There was also a difference between MSC-treated experimental diabetic rats and control rats in body weight (232.7 g ±19.7 vs 133.3g ±13.1, P < 0.05). Histological and morphometric analysis of the pancreas of experimental diabetic rats showed the presence and differentiation of transplanted MSCs into insulin-producing cells which evidenced by double-staining of anti-BrdU and insulin. Also, there were many small islets throughout the sections. Their mean area and diameter analysis revealed that they were smaller thancontrol islets (1835.7 ± 175.8 µm2 vs 13257.2 ± 1457.6 µm2; 43.5 ± 3.7 µm vs 119.9 ± 5.8 µm, respectively, P < 0.05). Conclusion: Allogeneic MSCs transplantation can reduce blood glucose level in recipient rats. A relatively small quantity of transplanted diabetic MSCs survive and transdifferentiate into insulin-producing cells in the pancreas of recipient rats. Upon transplantation these cells initiate endogenous pancreatic regeneration by neogenesis of islet of recipient origin. The present study demonstrates that diabetic MSCs retains its stemness and potential to induce pancreatic regeneration on transplantation.

Sign in / Sign up

Export Citation Format

Share Document