scholarly journals Prevalence of COVID-19 in 10,000 samples from 7853 patients in Eastern Turkey by positive real-time PCR

2021 ◽  
Author(s):  
Murat Karamese ◽  
Didem Ozgur ◽  
Ceyda Tarhan ◽  
Susamber D Altintas ◽  
Okan Caliskan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epidemiologic Studies ◽  
Screening Tests ◽  
Polymerase Gene ◽  
Positive Real ◽  
Final Study ◽  
The World ◽  
Study Population ◽  
Rna Polymerase Gene

Aim: COVID-19, caused by SARS-CoV-2, started in December 2019 and has spread across the world. Materials & methods: We analyzed real-time PCR results of 10,000 samples from 2 April to 30 May 2020 in three neighbor cities located in the East of Turkey. The final study population was 7853 cases, after excluding screening tests. Results: Real-time PCR was performed to detect the SARS-CoV-2-specific RNA-dependent-RNA-polymerase gene fragment. The number of total positive samples out of 7853 were 487; however, the number of nonrepeating positive patient was 373 (4.8%). Cough and fever were the most common symptoms in positive cases. Conclusion: Epidemiologic studies should be performed about the prevalence of SARS-CoV-2 infection to better understand the effect of the virus across the world.

scholarly journals The Prevalence of RT-PCR Positivity of SARS-CoV-2 on 10,000 Patients from Three Cities Located on the Eastern of Turkey

2020 ◽  
Author(s):  
Karamese Murat ◽  
Ozgur Didem ◽  
Tarhan Ceyda ◽  
Dik Altintas Susamber ◽  
Caliskan Okan ◽  
...  
Keyword(s):  
Epidemiologic Studies ◽  
Screening Tests ◽  
Gene Fragment ◽  
Polymerase Gene ◽  
Rt Pcr ◽  
Rna Dependent Rna Polymerase ◽  
Final Study ◽  
The World ◽  
Study Population ◽  
Rna Polymerase Gene

ABSTRACTCOVID-19, is caused by SARS-CoV-2, has been started on December/2019 in Wuhan/China and spread all over the world. We analyzed RT-PCR results of 10,000 cases from April-2 to May-30, 2020 in three neighbor cities located on the Eastern of Turkey. The final study population was 7853 cases after excluded screening tests. RT-PCR were performed to detect the SARS-CoV-2-specific RdRp (RNA-dependent-RNA-polymerase) gene fragment. The number of total positive samples out of 7853 were 487; however, the number of non-repeating positive patient was 373 (4.8%). The cough and fever were the most common symptoms in positive cases. The epidemiologic studies should be performed about the prevalence of SARS-CoV-2 infection to better understand the effect of the virus all over the world.

scholarly journals Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 656
Author(s):  
Konstantin Tanida ◽  
Andreas Hahn ◽  
Kirsten Alexandra Eberhardt ◽  
Egbert Tannich ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Real Time ◽  
Indigenous People ◽  
Real Time Pcr ◽  
Latent Class ◽  
Enterocytozoon Bieneusi ◽  
Enteric Disease ◽  
Immunosuppressed Patients ◽  
Study Population ◽  
Stool Samples ◽  
Pcr Assays

Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting Enterocytozoon bieneusi and Encephalocytozoon spp. were included in the assessment with reference stool material (20), stool samples from Ghanaian HIV-positive patients (903), and from travelers, migrants and Colombian indigenous people (416). Sensitivity of the assays ranged from 60.4% to 97.4% and specificity from 99.1% to 100% with substantial agreement according to Cohen’s kappa of 79.6%. Microsporidia DNA was detected in the reference material and the stool of the HIV patients but not in the stool of the travelers, migrants, and the Colombian indigenous people. Accuracy-adjusted prevalence was 5.8% (n = 78) for the study population as a whole. In conclusion, reliable detection of enteric disease-associated microsporidia in stool samples by real-time PCR could be demonstrated, but sensitivity between the compared microsporidia-specific real-time PCR assays varied.

scholarly journals Analysis of 721 Chlamydia trachomatis-positive urogenital specimens from men and women using LGV L2-specific real-time PCR assay

2006 ◽  
Vol 11 (42) ◽  
Author(s):  
D Goldenberger ◽  
F Dutly ◽  
M Gebhardt
Keyword(s):  
Chlamydia Trachomatis ◽  
Real Time ◽  
Real Time Pcr ◽  
Western Europe ◽  
Lymphogranuloma Venereum ◽  
Pcr Assay ◽  
Men And Women ◽  
The World ◽  
Sex With Men

The epidemic of rectal lymphogranuloma venereum among men who have sex with men in western Europe and other parts of the world is ongoing

scholarly journals Quantification and conceptual validation of the inoculum potential of Sclerotinia sclerotiorum in soybean and bean seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Sueny Kelly Santos de França ◽  
Carolina da Silva Siqueira ◽  
Marina de Resende Faria Guimarães ◽  
José da Cruz Machado
Keyword(s):  
Seed Germination ◽  
Real Time ◽  
Molecular Analysis ◽  
Sclerotinia Sclerotiorum ◽  
Real Time Pcr ◽  
White Mold ◽  
Inoculum Potential ◽  
Severe Damage ◽  
The World ◽  
The Relationship

Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout the world. The disease is considered to be one of the major diseases of soybean and bean crops in Brazil. The pathogen S. sclerotiorum is spread by soybean and bean seeds both in the form of sclerotia and dormant mycelium inside the seeds. The objective of this work was to evaluate the relationship between different potentials of S. sclerotiorum in soybean and bean seeds and the performance of these seeds, as well as to verify the localization and quantification of the inoculum of the pathogen in the seeds inoculated by Real-time PCR (qPCR), validating the term inoculum potential. Soybean and bean seeds were inoculated with the fungus by the osmotic conditioning method based on the exposure of the seeds to the fungus for periods of 24 h, 48 h, 72 h, and 96 h. Molecular analysis was carried out by means of qPCR in whole seeds and dissected in the integument, cotyledon and embryonic axis. The results showed that the effects of S. sclerotiorum on seed germination and vigor were progressive and proportional to the increases in inoculum potentials, since there was more severe damage to the seeds and consequently to the emerged plants at the highest potential (P96). The inoculum of the pathogen was found in all parts of the evaluated seeds, even at its lowest inoculum potential (P24), with an increasing DNA concentration, and the integument obtained a greater amount of DNA than the embryo, in comparison.

scholarly journals New reports of Australian cutaneous leishmaniasis in Northern Australian macropods

2009 ◽  
Vol 137 (10) ◽  
pp. 1516-1520 ◽  
Author(s):  
A. DOUGALL ◽  
C. SHILTON ◽  
J. LOW CHOY ◽  
B. ALEXANDER ◽  
S. WALTON
Keyword(s):  
Life Cycle ◽  
Cutaneous Leishmaniasis ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Findings ◽  
Northern Territory ◽  
Causative Organism ◽  
The World ◽  
Species Specific ◽  
Unique Species

SUMMARYCutaneous leishmaniasis caused by various species of Leishmania is a significant zoonotic disease in many parts of the world. We describe the first cases of Australian cutaneous leishmaniasis in eight northern wallaroos, one black wallaroo and two agile wallabies from the Northern Territory of Australia. Diagnosis was made through a combination of gross appearance of lesions, cytology, histology, direct culture, serology and a species-specific real-time PCR. The causative organism was found to be the same unique species of Leishmania previously identified in red kangaroos. These clinical findings provide further evidence for the continuous transmission of the Australian Leishmania species and its presence highlights the importance of continued monitoring and research into the life-cycle of this parasite.

scholarly journals Large-scale outbreak of Chikungunya virus infection in Thailand, 2018–2019

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247314
Author(s):  
Sarawut Khongwichit ◽  
Jira Chansaenroj ◽  
Thanunrat Thongmee ◽  
Saovanee Benjamanukul ◽  
Nasamon Wanlapakorn ◽  
...  
Keyword(s):  
South Asia ◽  
Real Time ◽  
Real Time Pcr ◽  
Large Scale ◽  
Chikungunya Virus ◽  
Rapid Test ◽  
Genomic Diversity ◽  
Likelihood Method ◽  
Positive Real ◽  
Antibody Titers

Between 2018 and 2019, the incidence of chikungunya was approximately 15,000 cases across 60 provinces in Thailand. Here, the clinical presentations in chikungunya, emergent pattern, and genomic diversity of the chikungunya virus (CHIKV) causing this massive outbreak were demonstrated. A total of 1,806 sera samples from suspected cases of chikungunya were collected from 13 provinces in Thailand, and samples were tested for the presence of CHIKV RNA, IgG, and IgM using real-time PCR, enzyme-linked immunoassay (ELISA), commercial immunoassay (rapid test). The phylogenetic tree of CHIKV whole-genome and CHIKV E1 were constructed using the maximum-likelihood method. CHIKV infection was confirmed in 547 (42.2%) male and 748 (57.8%) female patients by positive real-time PCR results and/or CHIKV IgM antibody titers. Unsurprisingly, CHIKV RNA was detected in >80% of confirmed cases between 1 and 5 days after symptom onset, whereas anti-CHIKV IgM was detectable in >90% of cases after day 6. Older age was clearly one of the risk factors for the development of arthralgia in infected patients. Although phylogenetic analysis revealed that the present CHIKV Thailand strain of 2018–2020 belongs to the East, Central, and Southern African (ECSA) genotype similar to the CHIKV strains that caused outbreaks during 2008–2009 and 2013, all present CHIKV Thailand strains were clustered within the recent CHIKV strain that caused an outbreak in South Asia. Interestingly, all present CHIKV Thailand strains possess two mutations, E1-K211E, and E2-V264A, in the background of E1-226A. These mutations are reported to be associated with virus-adapted Aedes aegypti. Taken together, it was likely that the present CHIKV outbreak in Thailand occurred as a result of the importation of the CHIKV strain from South Asia. Understanding with viral genetic diversity is essential for epidemiological study and may contribute to better disease management and preventive measures.

Effect of MCP-1 and CCR2 Genes Polymorphism on Development of Hepatocellular Carcinoma in HCV- Infected Patients in Sohag Governorate, Egypt

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 125-134
Author(s):  
Asmaa Nasr El-Din ◽  
Ghada M. Galal ◽  
Ahmed Abudeif ◽  
Marwa S. Hashem ◽  
Abeer Sheneef
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Real Time ◽  
Tumor Cells ◽  
Real Time Pcr ◽  
Primary Tumor ◽  
High Serum ◽  
Tumor Associated Macrophage ◽  
Common Cancer ◽  
The World

Background: HCC is the most common primary tumor of the liver .It is the fifth common cancer in men and the eighth common in women and is the second leading cause of cancer-related death in the world. The MCP-1 is a chemokine and a potent chemotactic factor for monocytes. MCP-1 expression in tumor cells is significantly linked to the extent of tumor-associated macrophage infiltration. Objectives: to detect the effect of MCP-1 and CCR2 Genes Polymorphism in development of HCC in HCV- related liver cirrhosis patients. Methodology: MCP-1-2518 A/G and CCR2 (V64Ile) genes polymorphism was assessed by real time PCR in 35 HCC patients and 30 HCV related LC. Serum MCP-1 was measured by ELISA. Results: For MCP-1 -2518 A G gene, HCC patients had a higher frequency of AG and GG genotypes than of AA genotype compared to other groups. For CCR2 (V64Ile), HCC patients had a higher frequency of GA and AA genotypes than GG genotype compared to other groups. Conclusion: There is a significant association between CCR2 (V64Ile) polymorphism, high serum MCP-1 level and HCC development in HCV- related liver cirrhosis patients.

TaqMan real-time PCR assay based on DNA polymerase gene for rapid detection of Orf infection

2011 ◽  
Vol 178 (1-2) ◽  
pp. 249-252 ◽  
Author(s):  
D.P. Bora ◽  
G. Venkatesan ◽  
V. Bhanuprakash ◽  
V. Balamurugan ◽  
M. Prabhu ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Polymerase Gene ◽  
Pcr Assay ◽  
Dna Polymerase Gene

scholarly journals Genotyping of the Lactase-Phlorizin Hydrolase −13910 Polymorphism by LightCycler PCR and Implications for the Diagnosis of Lactose Intolerance

2006 ◽  
Vol 52 (1) ◽  
pp. 148-151 ◽  
Author(s):  
Gerd Bodlaj ◽  
Markus Stöcher ◽  
Peter Hufnagl ◽  
Rainer Hubmann ◽  
Georg Biesenbach ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Lactose Intolerance ◽  
Strongly Correlated ◽  
Genotype Frequencies ◽  
Breath Hydrogen Test ◽  
Study Population ◽  
Caucasian Patients ◽  
Genotype C ◽  
Relationship Of

Abstract Background: Hypolactasia and lactose intolerance are common conditions worldwide. Hypolactasia seems to be strongly correlated with genotype C/C of the genetic variant C→T−13910 upstream of the lactase phlorizin hydrolase (LPH) gene. We developed a rapid genotyping assay for LPH C→T−13910 and investigated the relationship of positive lactose breath hydrogen test (LBHT) results suggesting lactose intolerance with LPH C→T−13910 genotype. Methods: Using automated DNA purification on the MagNA Pure LC and real-time PCR on the LightCycler, we examined samples from 220 individuals to estimate genotype frequencies; we then determined LPH C→T−13910 genotype in samples from 54 Caucasian patients with a positive LBHT result and symptoms of lactose intolerance. Results: Genotyping of 220 individuals revealed frequencies of 21.4%, 41.8%, and 36.8% for genotypes C/C, C/T, and T/T. Of the patients with positive LBHT results, only 50% had the C/C genotype suggestive of primary adult hypolactasia in our study population. The other patients had various degrees of secondary hypolactasia or symptoms of lactose intolerance. Patients with C/C genotype had a mean (SD) peak H2 increase in the LBHT [108 (58) ppm] that was significantly higher than in patients with the C/T [65 (54) ppm] and T/T [44 (34) ppm] genotypes. Conclusions: The new real-time PCR assay provides a rapid, labor-saving means for the genotyping of LPH C→T−13910. Use of the assay may assist in differentiating patients with primary hypolactasia from those with secondary hypolactasia and lactose intolerance, who may need further clinical examinations to diagnose their underlying primary diseases.

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