scholarly journals Aktivitas Antioksidan Si Ungu Mentawai

2020 ◽  
Vol 5 (1) ◽  
pp. 17
Author(s):  
Reny Salim ◽  
Suryani Suryani
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Acidic Solution ◽  
Antioxidant Power ◽  
Mentawai Islands ◽  
Antioxidant Content ◽  
Dpph Method ◽  
Ic50 Values

<p>Daun ungu merupakan salah satu tumbuhan yang telah cukup banyak diteliti kekuatan antioksidannya. Namun kandungan antioksidan pada tumbuhan sangat dipengaruhi oleh lingkungan tempat tumbuh dari tumbuhan tersebut. Pengaruh ini menimbulkan ketertarikan pada peneliti untuk meneliti kekuatan antioksidan dari daun ungu yang diambil dari kepulauan Mentawai. Penelitian tentang kekuatan antioksidan daun ungu ini dilakukan dengan cara daun ungu dimaserasi menggunakan pelarut etanol 70% dalam suasana sedikit asam, setelah itu dilanjutkan dengan fraksinasi menggunakan pelarut kloroform, etil asetat, dan air. Hasil ekstrak dan fraksi diteliti kekuatan antioksidannya dengan metoda DPPH. Hasil dari penelitian diperoleh nilai IC<sub>50</sub> ekstrak dan fraksi (kloroform, etil asetat, air) berturut-turut adalah (9; 138,56; 15,62; 16,65) µg/mL. Kategori kekuatan antioksidan dari ekstrak dan fraksi adalah kuat dan sedang.</p><p><em>Kata Kunci: aktivitas antioksidan, daun ungu, metoda DPPH</em></p><p><em>Purple leaves are one of the plants that have quite a lot of antioxidant power. However, the antioxidant content in plants varies greatly by the environment in which they are grown. This influence attracted interest in researchers to explore the antioxidant power of purple leaves taken from the Mentawai islands. Research on the antioxidant power of purple leaves was carried out by means of the purple leaves macerated using 70% ethanol solvent in a slightly acidic solution after that continued with fractionation using chloroform, ethyl acetate, and air solvents. The results of extracts and antioxidant extracts with the DPPH method. The results of the study obtained IC50 values of extracts and fractions (chloroform, ethyl acetate, air) respectively (9; 138.56; 15.62; 16.65) μg/mL. The category of antioxidant strength from extracts and fractions is strong and moderate.</em><em></em></p><p><strong><em>Keywords: </em></strong><em>antioxidant activity, leaf purple, DPPH method.</em></p>

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

scholarly journals Antioxidant Potential of Madura Knife Scallop (Solen sp) Extract as a Prevention of Oxidative Stress

2020 ◽  
Vol 15 (3) ◽  
pp. 382-389
Author(s):  
Mirwa Adiprahara Anggarani ◽  
Roy Januardi Irawan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Cell Structure ◽  
Oxygen Species ◽  
Meat Extract ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Reactive Molecule

Reactive Oxygen Species (ROS) are produced by humans as a result of cellular metabolism and environmental factors such as pollutants or cigarette smoke. ROS is a very reactive molecule and has the ability to damage cell structure. Oxidative stress is a condition between oxidants and antioxidants that is not balance, pathophysiologically, oxidative stress can trigger the risk of various diseases including hypertension, atherosclerosis, diabetes, heart failure, stroke and other chronic diseases. Oxidative stress can be overcome by intake of antioxidants. The purpose of this study was to determine the antioxidant activity contained in the meat and shells of a knife scallop (Solen sp.) that lives in Bangkalan waters, Madura. The method used in this study is an analysis of antioxidant activity with the DPPH method. Knife scallop has antioxidant activity which is evident from the IC50 values obtained. IC50 values of the shell and meat extract with ethanol solvent were 489.56 ppm and 748.49 ppm. IC50 values of the shell and meat extract with ethyl acetate solvent were 916.43 ppm and 2045.93 ppm. While the IC50 value of the shell and meat extract with chloroform solvent was 119.37 ppm and 1692.80 ppm. Based on IC50 data of knife scallop shell and meat extracts on the 3 types of solvents, it can be concluded that knife scallop’s shell and meat extract with ethanol solvent has the greatest antioxidant activity compared to ethyl acetate and chloroform solvents.

scholarly journals Antioxidant, hypoglycemic, and hepatoprotective effect of aqueous and ethyl acetate extract of carob honey in streptozotocin -induced diabetic rats

2019 ◽  
Vol 12 (12) ◽  
pp. 1916-1923 ◽  
Author(s):  
Redouan El-Haskoury ◽  
Noori Al-Waili ◽  
Jaouad El-Hilaly ◽  
Waili Al-Waili ◽  
Badiaa Lyoussi
Keyword(s):  
Antioxidant Activity ◽  
Liver Function ◽  
Ethyl Acetate ◽  
Glucose Level ◽  
Ethyl Acetate Extract ◽  
Treated Group ◽  
Diabetic Rats ◽  
Antioxidant Power ◽  
Antioxidant Content

Aim: The aim of the study included the effect of aqueous extract (AE) and ethyl acetate extract (EAE) on blood sugar in diabetic rats and their effects on liver enzymes and lipid panel in control and diabetic rats. Furthermore, the antioxidant activity of the EAE was studied in vitro and compared with AE. Materials and Methods: Sugar and antioxidant content of AE and EAE were determined. In vitro antioxidant activity of AE and EAE was estimated by 2, 2-diphenyl-1-picrylhydrazyl and ABTS*+ radical scavenging assay, ferric-reducing antioxidant power assay, and total antioxidant assay. To study the effect of the extracts on blood glucose level (BGL), lipid profile, and liver function in non-diabetic and diabetic rats, five groups of six rats each were treated with distilled water, AE, EAE, glibenclamide (GLB), and sucrose for 8 days. Plasma glucose level (PGL), total cholesterol (TC), triglycerides (TG), transaminases (alanine transaminase [ALT] and aspartate transaminase [AST]), and alkaline phosphatase (ALP) were determined. The effect of the interventions on BGL after acute administration also was investigated. Diabetes was induced by streptozotocin injection. Results: EAE contains significantly lower content of fructose and glucose than AE (p<0.05), and it has no sucrose. AE and EAE exhibited a significant antioxidant activity and high antioxidant content; the antioxidant content was higher in AE than EAE (p<0.05). In diabetic rats, acute treatment by AE increased PGL, while EAE significantly lowered BGL as compared to the untreated diabetic rats. Both interventions significantly decreased BGL as compared to the sucrose treated group in diabetic rats (p<0.05). EAE was more potent than GLB. Sucrose caused 13% increment in BGL after 8 days of induction of diabetes, while AE caused only 1.3% increment. Daily treatment by EAE decreased significantly AST, ALT, ALP, and TC. EAE decreased significantly TC and TG level in diabetic rats in comparison to the untreated diabetic group. Conclusion: The study showed for the 1st time that EAE has more hypoglycemic effect than AE, and both extracts prevent the increment in BGL on day 8 after induction of diabetes observed in the control and sucrose treated group. EAE significantly ameliorated the lipid and liver function disorders induced by diabetes.

Antioxidant Activity of Pidada (Sonneratia caseolaris (L.) Engl.) Fruit Extract by DPPH Method

2020 ◽  
Author(s):  
Sari Defi Okzelia ◽  
Maulida Nurdaini
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Fruit Extract ◽  
Butanol Fraction ◽  
Sonneratia Caseolaris ◽  
Dpph Method ◽  
Ic50 Values ◽  
Positive Controls

Sonneratia caseolaris (L.) Engl. or commonly called pidada fruit is one type of mangrove thatis commonly found in the coastal areas of Muara Gembong, Bekasi, West Java. Pidadacontains vitamins and secondary metabolite compounds that are beneficial for health. Thepurpose of this study was to determine the concentration of ethanolic extract, n-hexanefraction, ethyl acetate fraction, and butanol fraction of pidada fruit that could inhibit 50%absorbance of DPPH as free radical. Extraction was conducted by maceration method using96% ethanol, then fractionated using n-hexane, ethyl acetate and butanol as therepresentatives of nonpolar, semipolar and polar solvent. This study used vitamin C andvitamin E as positive controls. The percentage of antioxidant activity was obtained from thedata absorbance. IC50 values obtained from linear regression between the concentration oftested series versus the percentage of antioxidant activity. The results showed that the ethanolextract of pidada fruit possessed very strong antioxidant activity while n-hexane fractionpossessed weak antioxidant activity, ethyl acetate fraction possessed strong antioxidantactivity, and butanol fraction possessed very weak antioxidant activity. IC50 values of ethanolextract, n-hexane fraction, ethyl acetate fraction, and butanol fraction of pidada fruit were32,58; 191,31; 96,02 and 371,16 ppm respectively.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BUAH PURNAJIWA (Kopsia arborea Blume.) DENGAN BERBAGAI PELARUT

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 24 ◽  
Author(s):  
Didit Purwanto ◽  
Syaiful Bahri ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Fruit Extract ◽  
Dpph Method ◽  
Ic50 Values

Antioxidant activity of Purnajiwa (Kopsia arborea Blume.) fruit has been investigated.The fruit was extracted by various solvents such as n-hexane, ethyl acetate, and ethanol. The aim of the research is to define a class of compounds and antioxidant activity. The compounds test was conducted by the phytochemical method. The results showed that the purnajiwa fruit extract is classified as secondary metabolites of flavonoids, saponins, tannins, alkaloids, and steroids. The antioxidant activity was tested by DPPH method and the amount of antioxidant activity was characterized by IC50 values. The result indicated that the antioxidant activity (IC50) for n-hexane, ethyl acetate, and ethanol solvents is 3524.05 ppm, 316.09 ppm, and 154.89 ppm respectively.Keywords: Purnajiwa Fruit, Antioxidant, DPPH, Phytochemicals

scholarly journals IN VITRO ANTI-WRINKLE AND TYROSINASE INHIBITORY ACTIVITIES OF GRAPEFRUIT PEEL and STRAWBERRY EXTRACTS

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Endang Lukitaningsih ◽  
Syamsu Nur ◽  
Fadilah Qonithah ◽  
Andi Zulbayu ◽  
Rina Kuswahyuning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Grapefruit Peel ◽  
Ferric Reducing Antioxidant Power ◽  
Tyrosinase Enzyme ◽  
Collagenase Inhibition

The research aims to analyse the antioxidant, anti-tyrosinase and anti-wrinkle activities from grapefruit (Citrus Maxima L) and strawberry extracts. Samples were extracted by maceration using 96% ethanol and ethyl acetate, subsequently. The Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching (BCB) were used to measure the antioxidant activity. The effect of anti-wrinkle was determined by testing the inhibition of elastase and collagenase enzyme, while anti-tyrosinase activity was analysed using mushroom tyrosinase enzyme. The results showed that strawberry extracts in ethanolic (SE) and ethyl acetate (SEA) have antioxidant activity in FRAP (EC50 = 404.39 ± 3.27 µg / mL and 1978.65 ± 37.25 µg/mL) and BCB (IC50 = 292.30 ± 4.69 µg/mL and 671.11 ± 6.74 µg/mL). Whereas the grapefruit peel extracts both in ethanolic (GPE) and ethyl acetate (GPEA) have antioxidant activity in FRAP (EC50 219.47 ± 71.96 µg / ml and 309.44 ± 95.76 µg/ml) and BCB (EC50 245.19 ± 162.47 µg/ml and 567.54 ± 95.31 µg/ml). As positive standards for FRAP antioxidant analysis were quercetin and vitamin C which has IC50 respectively 18.97 ± 4.50 µg/mL and 24.47 ± 1.44 µg/mL. While in BCB analysis, Butylated Hydroxy Toluene (BHT) used as positive standard (IC50 38.68 ± 5.70 µg/mL). The samples of SE, SEA, GPE and GPEA showed tyrosinase inhibitory activity which the IC50 values were respectively 492.68 ± 1.43; 2658 ± 48.08; 3312.5 ± 222.74; 2985.17 ± 122.80 µg/ml. Kojic acid (IC50 111.52 ± 0.42 µg/ml) is used as positive standard in this study. In addition, SE, SEA, GPE and GPEA were able to inhibit elastase and collagenase enzymes, although their activities were still lower than the positive standard used in this study. Elastastinal in concentration 50 µg/mL giving elastase inhibition about 71.71 ± 0.81 µg/mL, while vitamin C in the same concentration showed collagenase inhibition about 66.79 ± 1.23 µg/mL. It can be concluded that the extract of strawberry and grapefruit peel has antioxidant, anti-tyrosinase and anti-wrinkle activity through inhibition of elastase and collagenase enzymes; thus, they can be used as antiaging cosmetic ingredients.

Antioxidant Activity of Non-Volatile Lime (Citrus aurantifolia Swingle) Extract

2021 ◽  
Vol 5 (2) ◽  
pp. 122
Author(s):  
Nandang Permadi ◽  
Euis Julaeha ◽  
Yudi Rosandi ◽  
Mohamad Nurzaman
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Volatile Fraction ◽  
Inhibition Concentration ◽  
Dpph Method ◽  
Ic50 Values ◽  
Lime Peel ◽  
Lime Production ◽  
By Products

The paper reports the antioxidant activity of a non-volatile fraction of lime processing byproducts from the lime syrup home industry. The activity was measured by spectrophotometry to obtain the 50% inhibition concentration (IC50) using the 2,2- diphenyl-1-picrylhydrazyl (DPPH) method. The sample was extracted by the maceration method with n-hexane, ethyl-acetate, and ethyl-ethanol. The IC50 values of 681 ppm, 458 ppm, and 2,775 ppm were n-hexane, ethyl-acetate, and ethyl-ethanol. The experiments concluded that the ethyl-acetate extract provides the best inhibition value to scavenge free-radicals DPPH. The HPLC and mass spectroscopy were measured to find out the content and group of active compounds. The significant compounds consisted of naringin, poncirin, or neoponcirin, which are known as antioxidant substances. The result shows the potential application of the lime by-products, its volatile fraction, and the nonvolatile fraction, which is the production residue of lime peel. This work can be applied as an alternative to zero-waste lime production, which may benefit the industry and the environment.

Photostability Study on Character and Antioxidant Activity of Tomato Extract (Solanum lycopersicum l.) in Nanostructured Lipid Carrier (NLC) and Conventional Creame

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Noorma Rosita ◽  
Dewi Haryadi ◽  
Tristiana Erawati ◽  
Rossa Nanda ◽  
Widji Soeratri
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Nanostructured Lipid Carrier ◽  
Uvb Radiation ◽  
K Value ◽  
Antioxidant Power ◽  
Solanum Lycopersicum L ◽  
Tomato Extract ◽  
Dpph Method ◽  
High Shear Homogenization

The aim of this study was to investigate the ability of NLC in increasing photostability of tomato extract in term of antioxidant activity. Photostability testing on antioxidant activity of samples were conducted by accelerating method using UVB radiation 32.400 joule for 21 hours radiation. Antioxidant activity was measured by DPPH method. NLC was made by High Shear Homogenization (HPH) method at 24000 rpm for 4 cycles, while conventional creame was made by low speed at 400 rpm. The product were characterized include: pH, viscosity, and particle size. There were had difference characters and physical stability. NLC had smaller size, more homogenous and more stable than conventional creame. It was known that stability of antioxidant activity of tomato extract in NLC system higher than in conventional creame. That was showed with k value, as constanta of rate scavenging activity decreasing in antioxidant power between time (Sigma 2-tail less than 0.005) of NLC and conventional creame were: 2.03x10-2 %/hour ±0.08 (3.94) and 4.71x 10-2 %/ hour ±0.23 (4.88) respectively.

scholarly journals Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2555
Author(s):  
Sang Koo Park ◽  
Yoon Kyung Lee
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyphenol Content ◽  
Abts Assay ◽  
Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

scholarly journals Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
Pattamaporn Aksornchu ◽  
Netima Chamnansilpa ◽  
Sirichai Adisakwattana ◽  
Thavaree Thilavech ◽  
Charoonsri Choosak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Digestive Enzyme ◽  
Radical Scavenging ◽  
Protein Glycation ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Values

Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

Sign in / Sign up

Export Citation Format

Share Document