Antioxidant Potential of Madura Knife Scallop (Solen sp) Extract as a Prevention of Oxidative Stress

Reactive Oxygen Species (ROS) are produced by humans as a result of cellular metabolism and environmental factors such as pollutants or cigarette smoke. ROS is a very reactive molecule and has the ability to damage cell structure. Oxidative stress is a condition between oxidants and antioxidants that is not balance, pathophysiologically, oxidative stress can trigger the risk of various diseases including hypertension, atherosclerosis, diabetes, heart failure, stroke and other chronic diseases. Oxidative stress can be overcome by intake of antioxidants. The purpose of this study was to determine the antioxidant activity contained in the meat and shells of a knife scallop (Solen sp.) that lives in Bangkalan waters, Madura. The method used in this study is an analysis of antioxidant activity with the DPPH method. Knife scallop has antioxidant activity which is evident from the IC50 values obtained. IC50 values of the shell and meat extract with ethanol solvent were 489.56 ppm and 748.49 ppm. IC50 values of the shell and meat extract with ethyl acetate solvent were 916.43 ppm and 2045.93 ppm. While the IC50 value of the shell and meat extract with chloroform solvent was 119.37 ppm and 1692.80 ppm. Based on IC50 data of knife scallop shell and meat extracts on the 3 types of solvents, it can be concluded that knife scallop’s shell and meat extract with ethanol solvent has the greatest antioxidant activity compared to ethyl acetate and chloroform solvents.

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Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2019.v5.i2.13671 ◽

2019 ◽

Vol 5 (2) ◽

pp. 189-197 ◽

Cited By ~ 1

Author(s):

Asriullah Jabbar ◽

Wahyuni Wahyuni ◽

Muh Hajrul Malaka ◽

Apriliani Apriliani

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Typhoid Fever ◽

Skin Diseases ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Ic50 Values ◽

Ethanol Extracts ◽

Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

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Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Jurnal Profesi Medika Jurnal Kedokteran dan Kesehatan ◽

10.33533/jpm.v15i1.2820 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Irfan Zamzani ◽

Nita Triadisti

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Methanol Extract ◽

Oxidation Process ◽

Antioxidant Activities ◽

Alternative Source ◽

Dpph Method ◽

Indigenous Plant ◽

Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽

10.35799/pha.8.2019.29297 ◽

2019 ◽

Vol 8 (2) ◽

pp. 315

Author(s):

Selin Widjaya ◽

Widdhi Bodhi ◽

Adithya Yudistira

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Brine Shrimp ◽

Ethanol Extract ◽

Brine Shrimp Lethality ◽

Phytochemical Content ◽

Lc50 Value ◽

Ic50 Value ◽

Dpph Method ◽

Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol. Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

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Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

Indo J Chem Res ◽

10.30598//ijcr.2019.7-fmd ◽

2019 ◽

Vol 7 (1) ◽

pp. 86-91

Author(s):

Fredryk W Mandey ◽

Endah Handayani ◽

Wahyuni Eka Nanda ◽

Alfian Noor

Keyword(s):

Antioxidant Activity ◽

Water Extract ◽

Hexane Extract ◽

Steroid Alkaloid ◽

South Sulawesi ◽

Ic50 Value ◽

Dpph Method ◽

Ic50 Values ◽

Positive Results

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

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Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽

10.22487/kovalen.2020.v6.i3.13662 ◽

2020 ◽

Vol 6 (3) ◽

pp. 198-205

Author(s):

Nur Faidah ◽

Nurhaeni ◽

Ahmad Ridhay ◽

Jusman ◽

Abd. Rahman Razak ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Allium Cepa ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Root Extract ◽

Polar Solvents ◽

Ic50 Value ◽

Dpph Method ◽

Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

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Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

Indo J Chem Res ◽

10.30598//ijcr.2020.7-fmd ◽

2019 ◽

Vol 7 (1) ◽

pp. 86-91

Author(s):

Fredryk W Mandey ◽

Endah Handayani ◽

Wahyuni Eka Nanda ◽

Alfian Noor

Keyword(s):

Antioxidant Activity ◽

Water Extract ◽

Hexane Extract ◽

Steroid Alkaloid ◽

South Sulawesi ◽

Ic50 Value ◽

Dpph Method ◽

Ic50 Values ◽

Positive Results

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AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽

10.33751/ekol.v19i1.1660 ◽

2020 ◽

Vol 19 (1) ◽

pp. 34-38

Author(s):

Siti Warnasih ◽

Diana Widiastuti ◽

Uswatun Hasanah ◽

Laksmi Ambarsari ◽

Purwantiningsih Sugita

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Soxhlet Extraction ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Palm Fruit ◽

Ic50 Value ◽

Dpph Method ◽

Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

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PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33524 ◽

2019 ◽

pp. 1-6

Author(s):

FONA QORINA ◽

ADE ARSIANTI ◽

QOTRUNNADA FITHROTUNNISA ◽

NADZILA ANINDYA TEJAPUTRI

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Folk Medicine ◽

Parasitic Infection ◽

Ethanolic Extract ◽

Positive Control ◽

Annona Muricata ◽

Tropical Plant ◽

Ic50 Value ◽

Dpph Method

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

Acta Biochimica Indonesiana ◽

10.32889/actabioina.v3i2.51 ◽

2021 ◽

Vol 3 (2) ◽

pp. 53-61

Author(s):

Angga Crystal Loasana Yami ◽

Irmanida Batubara ◽

Kholis Abdurachim Audah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Vibrio Cholerae ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Antibacterial Activities ◽

Diffusion Method ◽

Ic50 Value ◽

Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

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Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v1i2.433 ◽

2018 ◽

Vol 1 (2) ◽

pp. 41-47

Author(s):

Poppy Anjelisa Zaitun Hasibuan ◽

Mardiana

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Ethanol Extract ◽

Positive Control ◽

Hexane Extract ◽

Phytochemical Screening ◽

Ic50 Value ◽

Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories. Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

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