Antioxidant Activity of Non-Volatile Lime (Citrus aurantifolia Swingle) Extract

The paper reports the antioxidant activity of a non-volatile fraction of lime processing byproducts from the lime syrup home industry. The activity was measured by spectrophotometry to obtain the 50% inhibition concentration (IC50) using the 2,2- diphenyl-1-picrylhydrazyl (DPPH) method. The sample was extracted by the maceration method with n-hexane, ethyl-acetate, and ethyl-ethanol. The IC50 values of 681 ppm, 458 ppm, and 2,775 ppm were n-hexane, ethyl-acetate, and ethyl-ethanol. The experiments concluded that the ethyl-acetate extract provides the best inhibition value to scavenge free-radicals DPPH. The HPLC and mass spectroscopy were measured to find out the content and group of active compounds. The significant compounds consisted of naringin, poncirin, or neoponcirin, which are known as antioxidant substances. The result shows the potential application of the lime by-products, its volatile fraction, and the nonvolatile fraction, which is the production residue of lime peel. This work can be applied as an alternative to zero-waste lime production, which may benefit the industry and the environment.

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Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Jurnal Profesi Medika Jurnal Kedokteran dan Kesehatan ◽

10.33533/jpm.v15i1.2820 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Irfan Zamzani ◽

Nita Triadisti

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Methanol Extract ◽

Oxidation Process ◽

Antioxidant Activities ◽

Alternative Source ◽

Dpph Method ◽

Indigenous Plant ◽

Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽

10.22487/kovalen.2020.v6.i3.13662 ◽

2020 ◽

Vol 6 (3) ◽

pp. 198-205

Author(s):

Nur Faidah ◽

Nurhaeni ◽

Ahmad Ridhay ◽

Jusman ◽

Abd. Rahman Razak ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Allium Cepa ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Root Extract ◽

Polar Solvents ◽

Ic50 Value ◽

Dpph Method ◽

Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

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PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT PROPERTIES BY DPPH RADICAL SCAVENGER ACTIVITY OF RUELLIA BRITTONIANA FLOWER

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33531 ◽

2019 ◽

pp. 24-28

Author(s):

NADZILA ANINDYA TEJAPUTRI ◽

ADE ARSIANTI ◽

FONA QORINA ◽

QOTRUNNADA FITHROTUNNISA

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antioxidant Properties ◽

Chemical Components ◽

Phytochemical Analysis ◽

Radical Scavenger Activity ◽

Scavenger Activity ◽

Dpph Method ◽

Tlc Analysis

Objective: The genus Ruellia has been widely used in traditional and Ayurvedic medicine as an antioxidant. This study seeks to examine the antioxidant activity of the species Ruellia brittoniana. Methods: In this study, Ruellia brittoniana flowers were acquired from Depok, West Java, Indonesia. The flowers were cleaned and ground to form a powder, then dissolved in hexane, ethanol and ethyl acetate solvents. These three extracts were then tested for phytochemicals and thin layer chromatography (TLC) analysis. Ethanol and ethyl acetate extracts were also analyzed for antioxidants using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: Phytochemical results from the three extracts proved that Ruellia brittoniana contains flavonoids, alkaloids, tannins, glycosides and triterpenes. These results are comparable to the results from TLC analysis, which showed the samples contained 4–5 chemical components. Furthermore, the best antioxidant activity resulted from the ethyl acetate extract of the Ruellia brittoniana flower with an IC50 value of 68.42 ppm. Conclusion: An ethyl acetate extract from the Ruellia brittoniana flower can be used as a natural source of additional antioxidants.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

Acta Biochimica Indonesiana ◽

10.32889/actabioina.v3i2.51 ◽

2021 ◽

Vol 3 (2) ◽

pp. 53-61

Author(s):

Angga Crystal Loasana Yami ◽

Irmanida Batubara ◽

Kholis Abdurachim Audah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Vibrio Cholerae ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Antibacterial Activities ◽

Diffusion Method ◽

Ic50 Value ◽

Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

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Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v1i2.433 ◽

2018 ◽

Vol 1 (2) ◽

pp. 41-47

Author(s):

Poppy Anjelisa Zaitun Hasibuan ◽

Mardiana

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Ethanol Extract ◽

Positive Control ◽

Hexane Extract ◽

Phytochemical Screening ◽

Ic50 Value ◽

Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories. Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

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UJI FITOKIMIA DAN PENENTUAN Inhibition Concentration 50% PADA BEBERAPA TUMBUHAN OBAT DI PULAU TIDORE

JURNAL ILMIAH SAINS ◽

10.35799/jis.14.2.2014.6063 ◽

2014 ◽

Vol 14 (2) ◽

pp. 95

Author(s):

Wiwin Abdullah ◽

Max Revolta J. Runtuwene ◽

Vanda Selvana Kamu

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Leaf Extract ◽

Ethanol Extract ◽

Seed Extract ◽

Test Results ◽

Active Compounds ◽

Inhibition Concentration ◽

Dpph Method ◽

Ic50 Values

ABSTRAK Penelitian ini dilakukan untuk mengetahui senyawa aktif yang terkandung melalui pengujian fitokimia dan aktivitas antioksidan pada tumbuhan obat di pulau Tidore. Uji aktivitas antioksidan menggunakan metode DPPH dilanjutkan dengan perhitungan Inhibition Concentration 50% (IC50). Hasil yang diperoleh adalah biji buah mojoi terkandung senyawa alkaloid dan saponin, buah coro terkandung alkaloid, flavonoid, dan saponin, pada daun ofo terkandung alkaloid, tanin, flavonoid, steroid, dan saponin dan pada rimpang kuso mafola terkandung alkaloid, tanin, flavonoid, steroid, dan saponin. Nilai IC50 sebagai berikut biji rimpang kuso mafola 37,30 ppm, buah coro 250,17 ppm, daun ofo 976,10 ppm dan buah mojoi 1001, 07 ppm. Kata kunci :Tumbuhan obat, DPPH, antioksidan dan uji fitokimia. PHYTOCHEMICALS TEST AND DETERMINATION Inhibition Concentration 50% ON SOME MEDICINAL PLANTS IN THE TIDORE ISLAND ABSTRACT This study was conducted to determine the active compounds contained in an assessment of phytochemical and antioxidant activity in the medicinal plants of Tidore island. The test antioxidant activity was used DPPH method. In the test results to the phytochemical , that mojoi fruit seeds contained alkaloids and saponins, fruit coro (alkaloids, flavonoids, and saponins), ofo leaves (alkaloids, tannins, flavonoids, steroids, and saponins) and ethanol extract of rhizome kusomafola (alkaloids, tannins, flavonoids, steroids, and saponins. In calculation of IC50 values for rhizome kusomafola 37.30 ppm, 250.17 ppm coro fruit, 976.10 ppm ofo leaf extract, and fruit seed extract mojoi 1001.07 ppm. Keywords: Medicinal plants, DPPH, antioxidant and phytochemical test.

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Antioxidant Potential of Madura Knife Scallop (Solen sp) Extract as a Prevention of Oxidative Stress

Jurnal Kesehatan Masyarakat ◽

10.15294/kemas.v15i3.19521 ◽

2020 ◽

Vol 15 (3) ◽

pp. 382-389

Author(s):

Mirwa Adiprahara Anggarani ◽

Roy Januardi Irawan

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Ethyl Acetate ◽

Cell Structure ◽

Oxygen Species ◽

Meat Extract ◽

Ic50 Value ◽

Dpph Method ◽

Ic50 Values ◽

Reactive Molecule

Reactive Oxygen Species (ROS) are produced by humans as a result of cellular metabolism and environmental factors such as pollutants or cigarette smoke. ROS is a very reactive molecule and has the ability to damage cell structure. Oxidative stress is a condition between oxidants and antioxidants that is not balance, pathophysiologically, oxidative stress can trigger the risk of various diseases including hypertension, atherosclerosis, diabetes, heart failure, stroke and other chronic diseases. Oxidative stress can be overcome by intake of antioxidants. The purpose of this study was to determine the antioxidant activity contained in the meat and shells of a knife scallop (Solen sp.) that lives in Bangkalan waters, Madura. The method used in this study is an analysis of antioxidant activity with the DPPH method. Knife scallop has antioxidant activity which is evident from the IC50 values obtained. IC50 values of the shell and meat extract with ethanol solvent were 489.56 ppm and 748.49 ppm. IC50 values of the shell and meat extract with ethyl acetate solvent were 916.43 ppm and 2045.93 ppm. While the IC50 value of the shell and meat extract with chloroform solvent was 119.37 ppm and 1692.80 ppm. Based on IC50 data of knife scallop shell and meat extracts on the 3 types of solvents, it can be concluded that knife scallop’s shell and meat extract with ethanol solvent has the greatest antioxidant activity compared to ethyl acetate and chloroform solvents.

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Analisa Potensi Ekstrak Daun Kari (Murraya koenigii L. Spreng)

Talenta Conference Series Science and Technology (ST) ◽

10.32734/st.v2i1.300 ◽

2019 ◽

Vol 2 (1) ◽

pp. 1-8

Author(s):

Mustanir Mustanir ◽

Tara Rizki Al-Qarana ◽

Hilda Gusvianna ◽

Nurdin Saidi

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Murraya Koenigii ◽

E Coli ◽

Ic50 Values ◽

Hexane Extracts ◽

Inhibition Zones

Daun M. koenigii sangat umum digunakan sebagai rempah dalam berbagai masakan di Aceh, namun pemahaman masyarakat terhadap pentingnya daun M. koenigii perlu ditingkatkan agar pemanfaatannya dapat ditingkatkan. Dalam penelitian ini dilakukan uji aktivitas antibakterial daun M. koenigii terhadap bakteri E. coli dan S. aureus serta uji aktivitas antioksidan terhadap radikal bebas DPPH (1,1-Diphenyl-2-pycrilhydrazil). Uji antibakterial dilakukan dengan metode difusi cakram, dan hasilnya ekstrak etil asetat menghasilkan zona hambat yang lebih besar dibandingkan ekstrak metanol dan n-heksana. Zona hambat yang dihasilkan pada konsentrasi 1, 5 dan 10 % secara berturut-turut adalah 8,7; 7,7 dan 6,7 mm terhadap E. coli, dan 12,8 ;10,7 dan 8,0 mm terhadap S. aureus. Ekstrak etil asetat memiliki aktivitas antioksidan paling kuat dibandingkan dengan ekstrak metanol dan nheksana secara berturut-turut berdasarkan nilai IC50 yaitu 23; 50,54; dan 64,70 ppm. Berdasarkan kromatografi kolom diperoleh 8 (A-H) subfraksi dan subfraksi G menunjukan aktifitas antioksidan yang paling kuat sebesar 14,41 ppm Murraya koenigii (M. koenigi) leaves are very commonly used as spices in various cuisines in Aceh, but people's understanding of the importance of M. koenigi leaves needs to be improved, so it can be utilized better. In this study, the antibacterial activity of M. koenigii leaves was carried out against E. coli and S. aureus and antioxidant activity against DPPH free radicals (1,1- Diphenyl-2-pycrilhydrazil). Antibacterial tests were carried out using the disc diffusion method, and as a result, the ethyl acetate extract produced a larger inhibition zone than the methanol and n-hexane extracts. The inhibition zones produced at concentrations of 1, 5 and 10% were 8.7; 7.7 and 6.7 mm against E. coli, and 12.8; 10.7 and 8.0 mm against S. aureus. Ethyl acetate extract had the strongest antioxidant activity compared to methanol and nexexane extracts based on IC50 values, 23; 50; 54; and 64.70 ppm. Based on column chromatography obtained, 8 (A-H) subfraction and subfraction G showed the strongest antioxidant activity of 14.41 ppm.

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FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BATANG Chisocheton sp. (C.DC) Harms (Meliaceae)

CHEMISTRY PROGRESS ◽

10.35799/cp.13.2.2020.31672 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Dewa Gede Katja

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Extract ◽

Stem Bark ◽

Bark Extract ◽

Test Results ◽

Ic50 Value ◽

Dpph Method ◽

Stem Bark Extract

Tujuan penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dan aktivitas antioksidan dari setiap ekstrak kulit batang Chisocheton sp. C.DC Harms (Meliaceae). Hasil ekstraksi 200 g serbuk kulit batang Chisocheton sp. C.DC Harms (Meliaceae) dengan n-heksana, etil asetat dan metanol masing-masing dengan 2000 mL berturut-turut menghasilkan 7,193 g ekstrak pekat n-heksana, 8,798 g ekstrak pekat etil asetat dan 18,683 g ekstrak pekat metanol. Hasil uji fitokimia menunjukkan adanya golongan senyawa flavonoid, triterpenoid dan tanin. Hasil uji aktivitas antioksidan dengan metode DPPH menunjukkan bahwa ekstrak n-heksana memberikan nilai IC50 sebesar 337,28 µg/mL, ekstrak metanol sebesar 216,73 µg/mL, dan ekstrak etil asetat sebesar 199,89 µg/mL yang berarti etil asetat memiliki kemampuan yang paling besar dalam menangkap radikal bebas.ABSTRACTThe objective of this study was determine the class of secondary metabolite compounds and antioxidant activity of each stem bark extract of Chisocheton sp. C.DC Harms (Meliaceae). The results of the extraction of 200 g Chisocheton sp. C.DC Harms (Meliaceae) with n-hexane, ethyl acetate and methanol with 2000 mL each yielded 7,193 g of concentrated extract n-hexane, 8,798 g of concentrated extract of ethyl acetate and 18,683 g of concentrated methanol extract. Phytochemical test results showed the presence of flavonoid, triterpenoid and tannin compounds. The results of the antioxidant activity test using the DPPH method showed that the n-hexane extract gave an IC50 value of 337,28 µg/mL, methanol extract was 216,73 µg/mL, and ethyl acetate extract was 199,89 µg/mL which means that ethyl acetate has the ability to greatest in capturing free radicals.

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IN VITRO SCREENING FOR ANTICHOLINESTERASE AND ANTIOXIDANT ACTIVITY OF EXTRACT OF GARCINIA ATROVIRIDIS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.24934 ◽

2018 ◽

Vol 11 (9) ◽

pp. 208

Author(s):

Chitra V ◽

Narayanan J

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Ethanolic Extract ◽

The Elderly ◽

Therapeutic Drug ◽

Brain Disorder ◽

Ic50 Values ◽

Dpph Scavenging

Objective: Garcinia atroviridis belongs to the family Clusiaceae. The whole plant was dried, extracted to obtain a xanthone-rich extract. Phytochemical studies of extract showed the presence of phenols, alkaloids, steroids, resins, and saponins. Alzheimer’s disease (AD) the most common form of dementia, a brain disorder which has been affecting the elderly. The natural sources such as plants are an abundant source having various medicinal properties.Methods: In the present study, G. atroviridis has been selected to test for the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity by Ellman’s method. The antioxidant activity has been performed by 2,2’-diphenyl-1-picrylhydrazyl radical (DPPH) assay.Results: The results showed that the extract from G. atroviridis could inhibit the activity of AchE and BuChE. The percentage inhibition of the ethanolic extract for AChE was found to be 69.4%, and ethyl acetate extract was found to be 75.6%, respectively. The percentage inhibition of the ethanolic extract for BuChE was found to be 59.4%, and ethyl acetate extract was found to be 63.2%, respectively. The IC50 values for DPPH scavenging activity were significant when compared with the standard ascorbic acid.Conclusion: The plant extract with significant antioxidant and anticholinesterase property has shown its potential to be investigated further as a therapeutic drug for AD.

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