Antioxidant Activity of Pidada (Sonneratia caseolaris (L.) Engl.) Fruit Extract by DPPH Method
Sonneratia caseolaris (L.) Engl. or commonly called pidada fruit is one type of mangrove thatis commonly found in the coastal areas of Muara Gembong, Bekasi, West Java. Pidadacontains vitamins and secondary metabolite compounds that are beneficial for health. Thepurpose of this study was to determine the concentration of ethanolic extract, n-hexanefraction, ethyl acetate fraction, and butanol fraction of pidada fruit that could inhibit 50%absorbance of DPPH as free radical. Extraction was conducted by maceration method using96% ethanol, then fractionated using n-hexane, ethyl acetate and butanol as therepresentatives of nonpolar, semipolar and polar solvent. This study used vitamin C andvitamin E as positive controls. The percentage of antioxidant activity was obtained from thedata absorbance. IC50 values obtained from linear regression between the concentration oftested series versus the percentage of antioxidant activity. The results showed that the ethanolextract of pidada fruit possessed very strong antioxidant activity while n-hexane fractionpossessed weak antioxidant activity, ethyl acetate fraction possessed strong antioxidantactivity, and butanol fraction possessed very weak antioxidant activity. IC50 values of ethanolextract, n-hexane fraction, ethyl acetate fraction, and butanol fraction of pidada fruit were32,58; 191,31; 96,02 and 371,16 ppm respectively.