Evaluation of pre-germination treatments in Carapa guianensis AUBL

Understanding seed germination of native forest species of the Tropical wet forest is essential for ecosystem regeneration and conservation, which have been affected by the exploitation of wood and non-wood forest products. Carapa guianensis Aulb. (Meliaceae) has been exploited due to the high quality of its wood and seeds. Particularly, the latter is a source of a medicinal oil of high value to the pharmaceutical industry. The indiscriminate use of this species has reduced its population size; therefore, scientific knowledge of its sexual propagation in nurseries is vital. This research aimed to evaluate seed germination of C. guianensis under different pre-germination treatments and substrates at the Centro de Investigación El Mira of AGROSAVIA. Pre-germination assays were conducted in a split-plot design with a random distribution of four repetitions. The cumulative germination percentage (CGP) and mean germination time (t50) were calculated and analyzed through an ANOVA, and Tukey’s multiple comparison test using InfoStat V.2016. Significant differences for CGP among treatments (p<0.0001) and interactions (p<0.0096) were found. The highest germination percentage (61%) was achieved in S1+T4; however, the T50 did not show significant differences (p=0.24). This research demonstrates that the highest germination percentage is achieved through a scarification treatment with imbibition in sand substrate under the agroclimatic conditions of Tumaco.

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Can Bulk and Nanosized Titanium Dioxide Particles Improve Seed Germination Features of Wheatgrass (Agropyron desertorum)

Notulae Scientia Biologicae ◽

10.15835/nsb539072 ◽

2013 ◽

Vol 5 (3) ◽

pp. 325-331 ◽

Cited By ~ 13

Author(s):

Reyhaneh AZIMI ◽

Hassan FEIZI ◽

Mohammad KHAJEH HOSSEINI

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Germination Time ◽

Positive Effects ◽

Tio2 Particles ◽

Nanosized Titanium Dioxide ◽

Mean Germination Time ◽

Germination Value ◽

Improve Seed Germination ◽

Nanosized Tio2

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.

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Dispersal and germination syndromes of tree seeds in a monsoonal forest in northern India

Seed Science Research ◽

10.1079/ssr2004193 ◽

2005 ◽

Vol 15 (1) ◽

pp. 29-42 ◽

Cited By ~ 8

Author(s):

R.C. Thapliyal ◽

S.S. Phartyal

Keyword(s):

Seed Germination ◽

Seed Storage ◽

Germination Percentage ◽

Cold Season ◽

Poor Quality ◽

Northern India ◽

High Germination ◽

Monsoon Forest ◽

Mean Germination Time ◽

Pre Treatment

This paper describes the dispersal–germination characteristics of seeds of 77 native tree species in a seasonal monsoon forest in Uttaranchal state, northern India. Results indicate that 50% of the species dispersed in the hot, dry summer months, 18% during the rainy season, 23% during the cold season and the remainder in late spring. Germination tests on fresh and laboratory-stored seeds revealed a relationship between morphological features of the fruit and both germination percentage and mean germination time (MGT). Highest mean germination (50%) was for dry-dehiscent fruits with winged wind-dispersed seeds, followed by dry-dehiscent fruits with non-winged seeds (38%) and seeds of dry-indehiscent fruits (37%). Lowest germination (29%) was for seeds from fleshy or pulpy fruits. MGT followed the reverse course. Germination data for seeds stored dry in the laboratory during one seeding cycle indicated six patterns of seed germination: (1) average germination percentage of fresh seeds lower than that of stored seeds, indicating an after-ripening requirement; (2) initial high germination percentage followed by low values, indicating a steep to moderate decline in viability following harvest; (3) no germination after 1 or 2 months of seed storage, due to complete loss of viability, indicating short seed longevity; (4) fresh seed germination in some species equalled the average germination value of stored seeds, indicating constant germination for one whole seeding cycle; (5) germination of both fresh and stored seeds remained consistently low throughout the season, indicating a requirement for some kind of pre-treatment or having poor quality of seeds; (6) initial high germination followed by decline and again increase, showing a seasonal rhythm of germination.

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Morphological and germination response variability in seeds of wild yellow gentian (Gentiana lutea L.) accessions from northwest Spain

Botany ◽

10.1139/b2012-028 ◽

2012 ◽

Vol 90 (8) ◽

pp. 731-742 ◽

Cited By ~ 8

Author(s):

Félix Pérez-García ◽

Federico Varela ◽

M. Elena González-Benito

Keyword(s):

Seed Germination ◽

Water Content ◽

Germination Rate ◽

Seed Mass ◽

Germination Percentage ◽

Gentiana Lutea ◽

Moist Chilling ◽

Mean Germination Time ◽

Wild Accessions ◽

Seed Water Content

Gentiana lutea L. (yellow gentian, Gentianaceae) is an important medicinal plant under protection as endangered species in most European countries. The aim of this work was to evaluate variation in seed mass, seed water content, and seed germination among 56 wild accessions of G. lutea. The effect of gibberellic acid (GA3), putrescine, moist chilling, and level of ripeness of seeds on subsequent germination was also investigated. Seeds of G. lutea showed physiological dormancy (final germination percentages ranged from 0% to 11%, depending on the accession) and GA3 enhanced seed germination drastically in all the accessions. The highest germination (99%) of GA3-treated seeds was reached at 15 °C. Final germination percentage and germination rate (as expressed by mean germination time), as well as seed mass and seed water content, varied significantly among accessions. In general, 1 year moist chilling did not significantly enhance G. lutea seed germination. For most accessions, no significant differences were found between fully ripe seeds and less ripe seeds for seed water content, seed mass, and seed germination. Applications of GA3 were always most effective than those of putrescine for increasing seed germination.

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Effects of different levels of NaCl on the seed germination of Cyamopsis tetragonoloba L.

Bangladesh Journal of Botany ◽

10.3329/bjb.v49i3.49995 ◽

2020 ◽

Vol 49 (3) ◽

pp. 625-632

Author(s):

Muhammad Akram ◽

Mehwish Zahid ◽

Abu Bakr Umer Farooq ◽

Muhammad Nafees ◽

Atta Rasool

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Seed Vigor ◽

Stress Index ◽

Cyamopsis Tetragonoloba ◽

Vigor Index ◽

Seedling Length ◽

Salinity Levels ◽

Mean Germination Time ◽

Different Levels

Effects of four different levels of NaCl (05, 10, 15, 20 dS/m) on the seed germination of cluster bean (Cyamopsis tetragonoloba L.) cultivars were investigated. Only distilled water was used in the control. The experiment was conducted in the Petri dishes under laboratory condition and laid out in CRD comprising five replicates. The results indicated that the germination index (GI), seed vigor index (SVI), germination stress index and seedling length (radicle and plumule) decreased with increase of salinity levels and germination percentage (GP) was reduced significantly at the highest level of salinity (200 mM). However, mean germination time (MGT) and time taken for 50% germination (T50) increased by increasing salinity levels. Correlation coefficient between all possible combinations was estimated and the results indicated that GP, GI, MGT, T50, and SVI had significant positive or negative correlation with each other.

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SEED GERMINATION OF GUNDELIA TOURNEFORTII L. UNDER DIFFERENT DORMANCY BREAKING TREATMENTS

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no1.141 ◽

2021 ◽

Vol 21 (No 1) ◽

Author(s):

Vian Dler Ali ◽

Farhad Hassan Aziz

Keyword(s):

Seed Germination ◽

Tap Water ◽

Petri Dish ◽

Dry Weight ◽

Germination Percentage ◽

Dormancy Breaking ◽

Mean Germination Time ◽

Breaking Seed Dormancy ◽

Germination Value ◽

Peak Value

Gundelia tournefortii L. is adapted to mountains area climate, and the germination of this plant is not easily. This research conducted to study the effect of different seed treatments in various treatment durations in two experiments in petri dish in the laboratory and in seedling tray in the lath house to breaking seed dormancy and germination stimulus. Results indicated that there is significant enhancement of germination by all treatments and the maximum germination percentage was obtained by seed freezing 99.17% in the laboratory and 93.33% in the lath house for all treatment durations 12, 14, 48 and 72 hours. The other parameters of seed germination like germination speed, peak value, mean daily germination, mean germination time, germination value, radicle length, radicle elongation velocity, plumule length, plumule elongation velocity, seedling fresh and dry weight escalated by seed freezing and seed soaking in tap water at all soaking durations 12, 24, 48 and 72 hours. Gundelia tournefortii L. germinated seed was an anatomically examined by paraffin method and calculated diploid number of chromosomes 2n=2x=18 by aceto-carmine squash methods

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Sensitivity of Seed Germination to Temperature and Light in Helichrysum amorginum

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2020.0007 ◽

2020 ◽

Vol 77 (1) ◽

pp. 7

Author(s):

Sofia ARGYRIOU ◽

Panayiota PAPASTYLIANOU ◽

George STAVROPOULOS ◽

Angeliki KOUSTA ◽

Ioanna KAKABOUKI ◽

...

Keyword(s):

Seed Germination ◽

Seedling Survival ◽

Germination Percentage ◽

Effect Of Temperature ◽

Germination Time ◽

Randomized Design ◽

Perennial Shrub ◽

Mean Germination Time ◽

Lower Temperature ◽

Seed Propagation

Helichrysum amorginum is a perennial shrub, endemic on the Greek island of Amorgos and used for medicinal, cosmetic and ornamental purposes. Information concerning germination behavior and seedling survival is considered essential for the establishment and cultivation of wild species. The objective of this study was to investigate the effect of temperature and light on germination of Helichrysum amorginum seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at three different suboptimal germination temperatures (15, 20 and 25°C) in light and dark conditions for 10 days. Seed material was collected from two different fields of the island (Aegiali and Katapola). The germination performance was evaluated by final germination percentage and mean germination time. The effect of temperature on seed germination percentage and mean germination time was significant in all cases. The presence of light enhanced germination, especially at the optimum temperature of 20°C. In most cases the mean germination time increased at the lower temperature of 15°C. Seeds from Aegiali exhibited higher germination ability than the seed lot from Katapola. Knowledge of seed germination behaviour is of major importance in order to establish effective protocols for seed propagation in Helichrysum amorginum.

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Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days

Agronomy ◽

10.3390/agronomy10040516 ◽

2020 ◽

Vol 10 (4) ◽

pp. 516 ◽

Cited By ~ 1

Author(s):

Mihaiela Cornea-Cipcigan ◽

Doru Pamfil ◽

Cristian Radu Sisea ◽

Rodica Mărgăoan

Keyword(s):

Gibberellic Acid ◽

Ornamental Plants ◽

Germination Percentage ◽

Seedling Vigor ◽

Higher Plant ◽

Vigor Index ◽

Mean Germination Time ◽

Number Of Leaves ◽

Ornamental Crops

Cyclamen species (swinebread spp.) are perennial herbaceous plants which belong to the Primulaceae family, widely used as medicinal or ornamental plants. Currently, gibberellic acid (GA3), an environmentally friendly bioregulator is widely used to enhance the productivity and phenotypic characteristics of multiple ornamental crops. A dual experiment was conducted to evaluate the influence of gibberellic acid (50, 100 or 150 mg/L), on germination earliness of seeds, along with growth and ornamental quality of selected Cyclamen species. Additionally, a greenhouse experiment with long and short sunlight photoperiod was conducted to evaluate its importance in the development of Cyclamen plants. Our results indicated that under short sunlight exposure, seeds germinated earlier than those kept in long sunlight photoperiod. Plants treated with foliar applications of GA3 exhibited higher plant height, leaf area, number of leaves, root length and tuber development. Significant differences were found in germination percentage (GP), mean germination time (MGT) and seedling vigor index (SVI), within different GA3 supplementation and greenhouse sunlight intensity (short and long days). In conclusion, our study suggests that seedling vigor and ornamental quality can be increased by GA3 application, an economic strategy for enhancing germination and growth of selected Cyclamen species.

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The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

Vegetable Crops Research Bulletin ◽

10.2478/v10032-012-0008-z ◽

2012 ◽

Vol 76 (1) ◽

pp. 119-126 ◽

Cited By ~ 8

Author(s):

Maryam Haghighi ◽

Zahra Afifipour ◽

Maryam Mozafarian

Keyword(s):

Seed Germination ◽

Salinity Stress ◽

Seedling Growth ◽

Germination Rate ◽

Germination Percentage ◽

Percentage Germination ◽

Tomato Seeds ◽

Germination Characteristics ◽

Salinity Levels ◽

Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

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The effects of NACL, KCL and MGCL2 on the germination of Brassica rapa var. parachinensis seed

International Journal of Biological Research ◽

10.14419/ijbr.v4i1.5854 ◽

2016 ◽

Vol 4 (1) ◽

pp. 52 ◽

Cited By ~ 1

Author(s):

Mona Ghanad ◽

Rosimah Binit Nulit ◽

Rusea Go ◽

Christina Yong Seok Yien

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Seed Vigor ◽

Salinity Treatment ◽

Randomized Design ◽

Multiple Comparison Test ◽

Completely Randomized Design ◽

One Way Anova ◽

Range Test ◽

Germinated Seeds

This study was conducted to determine seed germination on the mechanisms of salinity stress in B. rapa var. parachinensis (Hong kong choy sum). The type of experiment was a completely randomized design with three salinity treatment NaCl, MgCl2, and KCl and minimum four replications was used. Salinity treatment consisted of 0 (control), 50, 100, 150, 200, mM concentrations. The experiment was carried out under 70% relative humidity at 23°C with a photoperiod of 8:16 h light/dark. The germinated seeds were counted every day and germination percentage, seed vigor, seed tolerance, length of seedling and biomass seedling was measured. The data were analyzed by one-way ANOVA multiple comparison test followed by Duncan’s multiple range test.

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SYNCHRONIZING THE in vitro GERMINATION OF Psidium guineense Sw. SEEDS BY MEANS OF OSMOTIC PRIMING

Revista Árvore ◽

10.1590/0100-67622016000400008 ◽

2016 ◽

Vol 40 (4) ◽

pp. 649-660 ◽

Cited By ~ 3

Author(s):

Márcia Adriana Carvalho dos Santos ◽

Mailson Monteiro do Rego ◽

Manoel Abilio de Queiroz ◽

Bárbara França Dantas ◽

Wagner Campos Otoni

Keyword(s):

Seed Germination ◽

Potassium Nitrate ◽

Germination Percentage ◽

In Vitro Germination ◽

Peg 6000 ◽

Caatinga Biome ◽

Germination Speed ◽

Mean Germination Time ◽

Positive Effect

ABSTRACT The Brazilian guava (Psidium guineense Swartz) is seed-propagated and, being native to the Caatinga biome, may frequently have uneven germination.Thus, we aimed to evaluate the synchronization of the in vitro seed germination of three accessions of the Brazilian guava, using water, polyethyleneglycol (PEG 6000), and potassium nitrate (KNO3) at different potentials and times of osmotic priming. Seeds from three accessions of the Brazilian guava (Y85, Y93,and Y97) from the UNEB/BA Germplasm Active Bank were subjected to the following pretreatments: -0.6, -1.0, -1.4, and -1,8 MPa PEG 6000; 10 and 20% KNO3 for 24h; 10 and 20% KNO3 for 48h; water for 24 and 48h; and non-primed seeds as the control. The experimental design was therefore a 10x3+1 factorial scheme. We assessed the germination percentage (G), mean germination time (MGT), germination speed (GS), and germination speed index (GSI). Data was subjected to analysis of variance followed by a means test (Duncan at 5% probability) and regression. There was interaction between the priming treatments and accessions for all evaluated features, except G. PEG 6000 decreased the MGT (from 6 to 8 days) and increased GS and GSI of seeds from all three accessions at potentials -1.0 to -1.5 MPa.Water-priming had a positive effect on MGT, GS, and GSI of accession Y85 seeds. KNO3 negatively affected germination of seeds from all three accessions. Thereby, we could synchronize seed germination of accessions Y85 and Y97 with PEG 6000.

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