scholarly journals Bradford assay as a high-throughput bioanalytical screening method for conforming pathophysiological state of the animal

2020 ◽  
Vol 10 (1-s) ◽  
pp. 105-110
Author(s):  
Abhishesh Kumar Mehata ◽  
Deepa Dehari
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Screening Method ◽  
Cost Effective ◽  
The Body ◽  
Total Protein Concentration ◽  
Protein Estimation ◽  
Essential Components ◽  
Bradford Assay ◽  
Different Tissues

Proteins are the essential components of the tissues that play a key role in the body. Its expression in the cell or tissue under a specified set of conditions and at a particular time regulates the different body conditions either as a normal body function or as a disease state. Protein is an important building block of muscles, skin, cartilage, bones and blood. Bradford assay is a reliable advanced and cost-effective protein estimation test for determining the exact concentration of protein in different tissues of the animal. In this study, we have taken a rat suffering from protein deficiency disorder and total protein concentration in the heart, brain, liver, blood and kidney was determined. It was found that the total protein concentration in different tissues of rat i.e., heart, brain, liver, plasma and kidney was found to be 8.39 ± 0.75, 10.46 ± 0.76, 6.74 ± 0.39, 8.12 ± 0.32 mg/g of tissue and 61.27 ± 0.95 mg/mL of plasma respectively (mean ± SEM). As compared to earlier published reports the total protein concentration in different tissues like hear, brain, liver and kidney found much lower to standard value as reported by Beyer, the reason behind obtaining this kind of results may be due to the presence of insufficient amount of the protein content in different tissue of animal as suffering from protein degeneration disorder. The rat was unable to digest and store the protein or catabolism was much faster than anabolism. Keywords: Anabolism, Bradford assay, Catabolism, Protein estimation.

Determination of Total Protein Concentration in Solution Using Gold Electrode Modified with Silver Nanoparticles

2014 ◽  
Vol 27 (1) ◽  
pp. 253-257 ◽  
Author(s):  
Patrick Marcel Seumo Tchekwagep ◽  
Charles Péguy Nanseu-Njiki ◽  
Emmanuel Ngameni ◽  
Ravi Danielsson ◽  
Thomas Arnebrant ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Total Protein ◽  
Gold Electrode ◽  
Protein Concentration ◽  
Total Protein Concentration

Albumin fraction and measurement of total protein concentration

1993 ◽  
Vol 264 (5) ◽  
pp. H1723-H1726 ◽  
Author(s):  
B. T. Peterson ◽  
R. W. Tate
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Gamma Globulin ◽  
Colorimetric Assay ◽  
Full Range ◽  
Computational Method ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Standard Curve ◽  
Protein Assays

The standard curve of a typical colorimetric assay for total protein is often nonlinear and dependent on the albumin fraction of the protein standard. We developed a simple mathematical transformation to make the standard curve linear and a computational method to correct for differences in albumin concentrations among the samples. This method uses data from total protein assays on two sets of standards (albumin and gamma globulin) and provides accurate measures of total protein over the full range of albumin fractions. Comparison of this two-standard method with the a method that uses only albumin as a standard shows that this method prevents physiologically significant overestimations in total protein concentration and calculated protein osmotic pressure differences in the lungs.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

Assay of cerebrospinal fluid protein: a rate biuret method evaluated.

1983 ◽  
Vol 29 (1) ◽  
pp. 126-129 ◽  
Author(s):  
P R Finley ◽  
R J Williams
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
Protein Concentration ◽  
Protein A ◽  
Colorimetric Method ◽  
Total Protein Concentration ◽  
Reaction Cell ◽  
Biuret Method ◽  
Automated Instrument ◽  
Cerebrospinal Fluid Protein

Abstract We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate formation, which is directly proportional to the total protein concentration in the sample. The linear range of measurement is 120 to 7500 mg/L. Day-to-day precision (CV) over the range of 150 to 1200 mg/L ranged from 15.2 to 2.3%. The method was unaffected by radical alteration of the albumin/globulin ratio, but there is a positive interference in the presence of hemoglobin, a suppression in the presence of bilirubin, and no effect by xanthochromia. The method is precise, accurate, rapid, and convenient. The method was compared with the trichloroacetic acid method as performed on the Du Pont aca III, giving a correlation coefficient (r2) of 0.9693. The method is precise, accurate, rapid, and convenient.

PAROTID SECRETION OF PROTEIN IN MAN

1958 ◽  
Vol 36 (10) ◽  
pp. 1001-1008 ◽  
Author(s):  
Marion H. Ferguson ◽  
H. P. Krahn ◽  
J. A. Hildes
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Serum Proteins ◽  
Amylase Activity ◽  
Early Morning ◽  
Total Protein Concentration ◽  
Zone Electrophoresis ◽  
Residual Radioactivity ◽  
Unstimulated Saliva ◽  
Protein Components

In unstimulated saliva, total protein concentration averaged 186 mg per 100 ml and amylase activity 146 units per 100 ml. The protein concentration was lower in the early morning than at midday. After dilute acetic acid stimulation, both total protein concentration and amylase activity were increased but the concentrations were not affected by rates of secretion above 0.1 ml per minute. Unlike protein, the potassium concentration fell with stimulation.Using zone electrophoresis on filter paper, as many as nine protein components were found, none of which corresponded to the serum proteins. The amylase activity was restricted to a component of low mobility which moved to the anode. There were two or three bands containing glycoproteins; all moved towards the cathode. There were qualitative and quantitative differences between stimulated and unstimulated secretions. Saliva collected 2 or 24 hours after a tracer dose of I131 showed less than 1% residual radioactivity after dialysis or treatment with an anion exchange resin, indicating that little if any of the salivary iodide is organically bound.

Effect of protein concentration on the determination of digoxin in serum by fluorescence polarization immunoassay.

1984 ◽  
Vol 30 (11) ◽  
pp. 1826-1829 ◽  
Author(s):  
W H Porter ◽  
V M Haver ◽  
B A Bush
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Fluorescence Polarization ◽  
Protein Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Fluorescence Polarization Immunoassay ◽  
Total Protein Concentration ◽  
Protein Pellet

Abstract Determination of digoxin by fluorescence polarization immunoassay (FPIA) with the Abbott "TDx" is significantly influenced by the concentration of total serum protein. Each 10 g/L increase in serum protein results in an 8% decrease in measured digoxin. Studies with [3H]digoxin confirmed that digoxin binds to the protein pellet during the trichloroacetic acid precipitation step before the immunoassay. Serum protein, or equal concentrations of albumin or gamma-globulin, exert an equivalent effect on the apparent digoxin value. Because the total protein concentration of the assay calibrators is low (50 g/L) compared with its reference interval in serum (60-80 g/L), results by FPIA may be expected to be low by an average of 16% (range, 8-24%). Digoxin results by FPIA will be most nearly accurate when the calibrators include a total protein concentration of about 70 g/L. Patients' specimens with abnormally high or low protein content will give falsely high or low results for digoxin.

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