scholarly journals Assessment of anti bacterial screening of Pongamia pinnata stem against bacterial species: An In-vitro approach

2019 ◽  
Vol 9 (1-s) ◽  
pp. 181-184
Author(s):  
Adithya Gadeela Manish ◽  
Nerella Mounika ◽  
Bakshi Vasudha ◽  
Boggula Narender
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Bacterial Species ◽  
Bacterial Activity ◽  
Pongamia Pinnata ◽  
Bacterial Strains ◽  
Agar Diffusion ◽  
Diffusion Technique

Numerous studies have shown that aromatic and medicinal plants are sources of diverse nutrient and non-nutrient molecules which protect the human body against various pathogens. Nature has been a source of medicinal agents for thousands of years and a large number of modern drugs have been isolated from natural sources. Herbal medicine is the oldest known healthcare system known to mankind. India has rich medicinal plants of nearly 7500 species. Many medicinal plants were with a long history of use in folk medicine against a variety of diseases. Recently, many researchers have taken a great interest on medicinal plants for their phytochemical constituents and biological activities including anti microbial activity. The anti bacterial activity of the ethanolic crude stem extract of Pongamia pinnata against four bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Escherichia coli) was investigated, using agar diffusion technique. At concentrations ranging from 10-40 mg/mL, the ethanolic crude extract showed activity against the four bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and E. coli) from 2 mm-20 mm, after 24 hours incubation. The present study showed the effectiveness of the crude plant extract against the tested bacterial strains and indicates the potential use of the extract as anti bacterial agent for the control of infectious diseases. Keywords:  Pongamia pinnata, anti bacterial activity, agar diffusion technique, bacterial strains, chloramphenicol.

scholarly journals Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Daniyar Sadyrbekov ◽  
Timur Saliev ◽  
Yuri Gatilov ◽  
Ivan Kulakov ◽  
Roza Seidakhmetova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Spatial Structure ◽  
Bacterial Strains ◽  
Gram Negative ◽  
X Ray ◽  
Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

scholarly journals Antimicrobial Screening of some Medicinal Plants against Selected Bacterial Species

2020 ◽  
Vol 13 (13) ◽  
pp. 20-23
Author(s):  
Pragya Sapkota ◽  
Sapana Bhattarai ◽  
Anup Muni Bajracharya ◽  
Pramesh Bahadur Lakhe ◽  
Nishant Shrestha
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Therapeutic Potential ◽  
Bacterial Species ◽  
Artemisia Vulgaris ◽  
Traditional Treatment ◽  
Euphorbia Hirta ◽  
Synthetic Drugs ◽  
Gram Positive Bacteria

Medicinal plants are used as traditional treatment for numerous human diseases. According to WHO, medicinal plants would be the best source to obtain a variety of drugs. Contrary to the synthetic drugs, antimicrobial of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. Present study was carried out on the screening of some medicinal plants against selected pathogenic organisms in the period of October 2017 to January 2018. A total of nine different medicinal plants were screened and evaluated for their antimicrobial activity against 10 bacterial species. Among them, Euphorbia hirta, Azadirachtaindica, Artemisia vulgaris were found to be effective against gram positive bacteria (Bacillus subtilis, Staphylococcus aureus, Methicillin resistant Staphylococcus aureus MRSA), whereas rest six medicinal plants (Aeglemarmelos, Justiciaadhatoda, Ficusreligiosa, Syzygiumcumini, Nyctanthes arbor and Meliaazedarach) were found to be ineffective against all the microorganisms (Bacillus subtilis, Escherichia coli, Klebsiellapneumoniae, Proteus vulgaris, Pseudomonas aerogenosa, Salmonella typhii, Shigelladysentriae, MRSA). The minimum bactericidal concentration (MBC) of Euphorbia hirta against Bacillus subtilis and Staphylococcus aureus was found to be 12.5mg/ml and MBC of Artemisia vulgaris against Bacillus subtilis and MRSA was also found to be 12.5 mg/ml while of Staphylococcus aureus was 25mg/ml. Similarly, the MBC of Azadirachta indica against Staphylococcus aureus was 25mg/ml.

scholarly journals Physico-chemical characterization and antibacterial activity of different types of honey tested on strains isolated from hospitalized patients

2016 ◽  
Vol 60 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Lia M. Junie ◽  
Mihaela L. Vică ◽  
Mirel Glevitzky ◽  
Horea V. Matei
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Physico Chemical

AbstractThe first aim of the study was to compare the antibacterial activity of several types of honey of different origins, against some bacterial resistant strains. The strains had been isolated from patients. The second aim was to discover the correlations between the antibacterial character of honey and the physico-chemical properties of the honey. Ten honey samples (polyfloral, linden, acacia, manna, and sunflower) from the centre of Romania were tested to determine their antibacterial properties against the following bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica serovar Typhimurium, Bacillus cereus, Bacillus subtilis, and Listeria monocytogenes. Bacterial cultures in nutrient broth and the culture medium Mueller-Hinton agar were used. The susceptibility to antibiotics was performed using the disk diffusion method. All honey samples showed antibacterial activity on the isolated bacterial strains, in particular polyfloral (inhibition zone 13-21 mm in diameter) - because it is the source of several plants, and manna (inhibition zone 13-19.5 mm in diameter), and sunflower (inhibition zone 14-18.5 mm in diameter). Pure honey has a significant antibacterial activity against some bacteria which are resistant to antibiotics. Bacterial strains differed in their sensitivity to honeys. Pseudomonas aeruginosa and Staphylococcus aureus were the most sensitive. The present study revealed that honey antibacterial activity depends on the origin of the honey. We also found that there was a significant correlation between antibacterial activity of honeys and the colour of the honey but not between acidity and pH. The statistical analysis showed that the honey type influences the antibacterial activity (diameter of the bacterial strains inhibition zones).

scholarly journals ANTIBACTERIAL ACTION OF PROPOLIS ON SELECTED BACTERIAL REFERENCE STRAINS

2020 ◽  
Vol 20 (1-2) ◽  
Author(s):  
Bojan GOLIĆ́ ◽  
Vesna KALABÁ ◽  
Tanja ILIĆ́ ◽  
Dragana KALABÁ ◽  
Nina ZRNIĆ́
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Skin Diseases ◽  
Bacterial Species ◽  
Antibacterial Action ◽  
Bacterial Strains ◽  
Disk Diffusion Test

Propolis alcoholic tincture is most commonly used propolis product on the market for the treatment of minor infections in the oral cavity; angina, some skin diseases etc. As propolis is still an unofficial drug in pharmacies, we tested its antimicrobial activity using a disk-diffusion test on six reference bacterial species-Salmonella Enterica WDCM 00030, Salmonella typhimurium WDCM 00031, Listeria monocytogenes WDCM 00020, Staphylococcus aureus WDCM 00032, Escherichia coli WDCM 00013 and Pseudomonas aeruginosa WDCM 00024. The aim of this paper was to examine the antibacterial action of alcoholic solution of propolis on reference bacterial cultures and to determine the type of action. Based on the obtained results, it can be concluded that the bacterial strains of Salmonella Enteritidis WDCM 00030, Salmonella Typhimurium WDCM 00031, Listeria monocytogenes WDCM 00020, Staphylococcus aureus WDCM 00032 and Pseudomonas aeruginosa WDCM 00024 are highly sensitive to the action of propolis alcoholic tincture.

scholarly journals Anti-oxidant and anti-bacterial activities of 11 Calophyllum species from Indonesia

2019 ◽  
Vol 1 (2) ◽  
pp. 1-12
Author(s):  
Jamilah Abbas
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Bacterial Activity ◽  
Extraction And Separation ◽  
Anti Oxidant ◽  
Xanthone Compound

Members of Calophyllum (Calophyllaceae) are commonly used as herbal medicine in Indonesia from the beginning of civilization. Due to their tremendous potential as a source for pharmaceutical industry, we selected 11 Calophyllum species for the current anti-oxidant and anti-bacterial studies, namely, Calophyllum euryphyllum, C. bicolor, C. flavoramulum, C incrassaptum, C. lowei, C. macrophyllum, C. nodosum, C. soulattri, C. tetrapterum, C. teysmannii, and C. tomentosum. The methods include Calophyllum stem bark extraction and separation into n-hexane, ethyl acetate, and butanol fractions, followed by the antioxidant analysis using DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging) and the anti-bacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. The results showed that n-hexane fraction of C. soulattri exhibited the best anti-oxidant activity (IC50 = 2.2 µg/m). This study also showed that a xanthone compound isolated from C. macrophyllum, namely, 1,8 - dihydroxy-6 methoxy xanthone exhibited the anti-oxidant activity with IC50 = 1.56 ppm.

scholarly journals Antibacterial and antibiofilm properties of Phlomis and Stachys species

2020 ◽  
Vol 49 (2) ◽  
pp. 257-263
Author(s):  
Aynur Aybey
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Bacterial Species ◽  
Bacterial Strains ◽  
Methanol Extracts ◽  
Good Antibacterial Activity ◽  
Antibiofilm Agents

The ethyl acetate and methanol extracts of Phlomis pungens var. pungens, P. nissolii, P. armeniaca and Stachys byzantina, S. cretica L. subsp. mersiaea and S. cretica L. subsp. Smyrnaea were evaluated for their antibacterial and antibiofilm activities. Five bacterial species were used are Bacillus subtilis, Pseudomonas aeruginosa, Shigella sonnei, Salmonella typhimurium and Yersinia enterocolitica. The ethyl acetate and methanol extracts of plant species showed good antibacterial activity against all bacterial strains. More significantly, ethylacetate and methanol extracts of all Phlomis species were found to be more effective on degredation of mature biofilm against all used bacterial strains than extracts of Stachys species. The findings of the present study highlight the promising role of Phlomis and Stachys extracts as new lead structures in the search for novel antibacterial and antibiofilm agents.

Synthesis, Characterization and Antimicrobial Activity of Bis-Acetylferrocene Schiff Base

2011 ◽  
Vol 396-398 ◽  
pp. 1875-1878 ◽  
Author(s):  
Yu Ting Liu ◽  
Hai Long Guo ◽  
Da Wei Yin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

Three bis-acetylferrocene schiff bases have been synthesized and characterized by IR, 1H NMR, and elemental analysis, the results conformed well with expected structures. The synthesized compounds were screened in vitro for their antimicrobial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that these compounds are show excellent antimicrobia activities against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi ,Bacillus subtilis,Staphylococcus aureus.

scholarly journals Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dereje Nigussie ◽  
Gail Davey ◽  
Belete Adefris Legesse ◽  
Abebaw Fekadu ◽  
Eyasu Makonnen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Bacterial Species ◽  
Wound Infections ◽  
Methanol Extracts

Abstract Background Patients with lymphoedema are at high risk of getting bacterial and fungal wound infections leading to acute inflammatory episodes associated with cellulitis and erysipelas. In Ethiopia, wound infections are traditionally treated with medicinal plants. Methods Agar well diffusion and colorimetric microdilution methods were used to determine the antibacterial activity of methanol extracts of the three medicinal plants against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shewanella alage, methicillin-resistant S. aureus ATCC®43300TM, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603, and Pseudomonas aeruginosa ATCC37853. Results The methanol extract of L. inermis leaves showed high activity against all tested bacterial species, which was comparable to the standard drugs. Similarly, the extracts of A. indica showed activity against all tested species though at higher concentrations, and higher activity was recorded against Streptococcus pyogenes isolates at all concentrations. However, the extract of A. aspera showed the lowest activity against all tested species except Streptococcus pyogenes isolates. The lowest minimum inhibitory concentration (MIC) was recorded with the extract of L. inermis against E. coli isolate and S. aureus ATCC 25923. Conclusion Methanol extracts of L. inermis, A. indica, and A. aspera leaves exhibited antimicrobial activity against selected bacterial isolates involved in wound infections, of which the methanol extracts of L. inermis exhibited the highest activity. The results of the present study support the traditional use of plants against microbial infections, which could potentially be exploited for the treatment of wound infections associated with lymphoedema.

scholarly journals Antagonistic Study of Lantana camara (Linn) against with Pathogenic Bacteria

1970 ◽  
Vol 7 (7) ◽  
pp. 64-67 ◽  
Author(s):  
Geeta Shrestha Vaidya ◽  
Nabin Bhattarai
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Organic Solvent ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Research Work ◽  
Lantana Camara ◽  
Bacterial Strains ◽  
Water Solvent

A research work of antagonistic effect of water solvent and organic solvent (Ethanol of different concentration 50 % and 100 %) of extracts of Lantana camara were studied against with pathogenic fifteen strains of bacteria.Among fifteen species, of bacteria most of them were inhibited by L.camara extracts and only two species such as Klebsella oxytoca and Klebsella pneumoniae did not showed antibacterial activity with same extract with same concentration. Extracts obtained from the organic solvent and water solvent showed the different antagonistic properties with the same bacterial strains. Those bacterial strains which were inhibited their growth by water solvent could not inhibited by organic solvent extracts. This depends on presence of polar and non-polar bioactive compounds in the extracts. It also depends on polar and non-polar solvents used for the plant extract. Organic solvent extracts showed antibacterial effect towards Pseudomonas aeruginosa, Staphylococcus sp., Bacillus cereus, Bacillus subtilis, Bacillus thurengiensis, Escherichia coli, Staphylococcus aureus, Proteus mirabilis and water solvent extracts showed antibacterial effect towards Pseudomonas aeruginosa, Staphylococcus sp., Citrobacter frundi, Proteus sp., Bacillus subtilis, Enterobacter aerogenes, Salmonella paratyphie, Staphylococcus aureus, Shigella dysenteria. Both solvent extracts showed high antibacterial effect towards Staph aureus, Staphylococcus sp. and Pseudomonas aeruginosa. Both plant extracts showed selective antibacterial effect with different strains of bacteria, which shows that these are confined to cure the same bacterial diseases. Key words: Lantana camara; Pathogenic bacteria; Antimicrobial; Organic and water solvent; Concentration. DOI: 10.3126/sw.v7i7.3828 Scientific World Vol.7(7) 2009 pp.64-67

scholarly journals The antimicrobial activity of leaves and callus extracts of Thevetia peruviana In vitro

2013 ◽  
Vol 7 (3) ◽  
pp. 74-80
Author(s):  
Salah K. M. Alhashimi ◽  
Khaleel I. Rashid ◽  
Ghoson S. Saleh ◽  
Alea M. Abdulhadi ◽  
Tara A. Taher
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Bacterial Species ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Thevetia Peruviana

The antimicrobial activity for Thevietia peruviana was evaluated by measuring inhibition zone diameter in agar using well diffusion assay. The aim of the present study was to evaluate the antimicrobial potential of Thevietia peruviana leaf extract as compared with callus extract against some bacterial strains and fungi. The results showed that the addition of 2,4-D at the concentration of 9 mg/l, and 0.1 mg/l of kinetin led to obtain callus weight reached 800 mg. It was noticed that the reduction of 2,4-D concentration up to 6 mg/l resulted in compact and green pieces of callus. The optimal weight and friable callus was obtained at 9 mg/l. Among the susceptible bacteria are the gram negative pseudomonas aeruginosa seemed to be sensitive against all concentration of Thevietia peruviana leaf and callus extracts, While Escherichia coli showed resistance with all concentrations of extracts. It was noted that the extracts were more active against gram positive staphylococcus aureus, as compared with other bacterial species. Results of this study revealed that callus extract of Thevetia peruviana possess higher activity in comparison with leaf extract against gram positive bacteria (Staphylococcus aureus, Bacillus cereus) and gram negative (Pseudomonas aeruginosa). Finally all the bioextracts were well stable at room temperature during the period of the study and did not show any reduction of activity against the bacterial strains used in this study experiments.

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