scholarly journals Fortifikasi senyawa selenium pada jamur tiram coklat (Pleurotus pulmonarius dan Pleurotus sajor-caju)

2020 ◽  
Vol 88 (1) ◽  
Author(s):  
Firda DIMAWARNITA ◽  
Yora FARAMITA ◽  
. TRI-PANJI
Keyword(s):  
Fruiting Body ◽  
Sodium Selenite ◽  
Food Source ◽  
Biological Efficiency ◽  
Growth Media ◽  
Efficiency Ratio ◽  
Pleurotus Pulmonarius ◽  
Oyster Mushrooms ◽  
Pleurotus Sajor Caju ◽  
High Concentrations

Selenium (Se) is one of the most important micronutrients needed for human health. However, the content of Se compound in animals and plants isrelativelysmallin order to meet recommended intakes of Se. Therefore, Se fortification in food source, such as mushroom, is needed. In this study, fortification was carried out by adding sodium selenite to the growth media (the mixture of sawdust and OPEFB) of brown oyster mushrooms included Pleurotus pulmonarius and Pleurotus sajor-caju, in various concentrations of 100, 200, 300, and 400 ppm. The results showed that the highest absorption of Se in P. pulmonarius and P. sojur caju (3.51 and 2.31 %, respectively) obtained from 200 ppm sodium selenite addition. High concentrations of sodium selenite in baglog media tend to inhibit mycellium growth and the production of mushroom fruiting body. The additions of 200 ppm sodium selenite in baglog media of P. pulmonarius and P. sajor-caju were the best treatment in term of the fastest mycelium coverage in 40 and 37 days with the highest biological efficiency ratio (BER) value of 18.80 and 17.89 %, respectively.Layu Senyawa selenium (Se) merupakan salah satu mikronutrien terpenting yang harus dipenuhi kebutuhannya dalam tubuh. Akan tetapi, kandungan senyawa Se pada hewan dan tumbuhan sangat kecil untuk memenuhi kebutuhan asupan Se yang dianjurkan. Oleh karena itu, fortifikasi Se pada sumber pangan seperti pada jamur diperlukan. Dalam penelitian ini, fortifikasi dilakukan dengan menambahkan sodium selenit ke dalam media pertumbuhan (campuran serbuk gergaji dan TKKS) jamur tiram coklat, yaitu Pleurotus pulmonarius dan Pleurotus sajor-caju dengan berbagai konsentrasi,diantaranya: 100, 200, 300, dan 400 ppm. Hasil penelitian menunjukkan penyerapan Se tertinggi pada P. pulmonarius dan P. sojurcaju yaitu masing-masingsebesar 3,51 dan 2,31% diperoleh dari penambahan sodium selenit 200 ppm. Konsentrasi sodium selenit yang tinggi pada media baglog cenderung menghambat pertumbuhan miselium dan produksi tubuh buah jamur. Penambahan sodium selenit sebanyak 200 ppm pada media baglog P. pulmonarius dan P. sajor-caju merupakan perlakuan terbaik ditinjau dari waktu tercepat pertumbuhan miselium jamur menutupi media dalam baglog, yaitu masing-masing 40 dan 37 hari dengan nilai biological efficiency ratio (BER) tertinggi, yaitu 18,80 dan 17,89 % secara berurutan.

scholarly journals The Effects of Sago (Metroxylon sagu) Bark and Frond Waste as Substrates on the Growth and Yield of Grey Oyster Mushrooms (Pleurotus sajor-caju)

2021 ◽  
Vol 44 (2) ◽  
Author(s):  
Haslinza Senghie ◽  
Mohamad Hasnul Bolhassan ◽  
Dayang Salwani Awg-Adeni
Keyword(s):  
Fruiting Body ◽  
Total Yield ◽  
Growth And Yield ◽  
Biological Efficiency ◽  
Significant Difference ◽  
Stipe Length ◽  
Insignificant Difference ◽  
Oyster Mushrooms ◽  
Pleurotus Sajor Caju ◽  
Metroxylon Sagu

This study was carried out to examine the effects of sago bark (SB) and sago frond (SF) waste on the growth and yield of grey oyster mushrooms (Pleurotus sajor-caju). Nine substrate formulas were studied, including sawdust (SD) alone as a control and the combination of 25:75, 50:50, and 75:25 ratios between SD and SB or SF. The results showed a significant difference in the total colonisation period, total fruiting body yield, and biological efficiency (BE). However, an insignificant difference was determined in the characteristics of the fruiting body for different substrate formulas. The substrates with the ratio of 50SD:50SF and 100 SD are the most suitable substrate formulas for the cultivation of P. sajor-caju. One hundred (100) SD achieved the fastest total colonisation period (24.44 days) but there was no significant difference with 75SD:25SF (24.78 days) and also obtained the fastest first harvest (50.33 days). However, 100SD produced a significantly lower total fruiting body yield (77.99 g/bunch) compared to 50SD:50SF, which produced the highest total yield (88.09 g/ bunch) and highest BE (17.62%) with a short total colonisation period (26.45 days). The substrates produced high values in cap diameter, stipe length, and effective fruiting bodies.

scholarly journals Pengaruh Komposisi Dedak Dan Ukuran Baglog Terhadap Produksi Jamur Tiram Putih (Pleurotus ostreatus)

2019 ◽  
Vol 8 (1) ◽  
pp. 38
Author(s):  
Sitti Nurlina ◽  
Tutik Nugrahini ◽  
Hamidah Hamidah
Keyword(s):  
Pleurotus Ostreatus ◽  
Block Design ◽  
Biological Efficiency ◽  
Average Weight ◽  
Efficiency Ratio ◽  
Fresh Weight ◽  
Randomized Block Design ◽  
Oyster Mushrooms ◽  
Four Levels

The study was conducted to determine the effect of bran composition and baglog size on the production of white oyster mushrooms (Pleurotus ostreatus). The study was arranged in a Randomized Block Design (RBD) consisting of 2 factors with 5 replications. The first factor is the composition of bran consisting of four levels, namely: 10%, 15%, 20% and 25%. The second factor is the size of baglog which consists of 4 levels, namely: 17 cm, 18 cm, 19 cm and 20 cm. The results showed that the composition of bran had a very significant effect on the age of the first harvest, average mushroom weight, average fresh weight per baglog and biological efficiency ratio. Baglog size has a significant effect on the age of the first harvest, average mushroom weight, average fresh weight per baglog and biological efficiency ratio. The best effect on the production of oyster mushrooms is produced by the composition of bran 20% (d3) and baglog size of 19 cm (b3). The interaction of bran composition and baglog size significantly affected the age of the first harvest and had a very significant effect on the average weight of mushrooms, but did not significantly affect the fresh weight of mushrooms per baglog and the ratio of biological efficiency. The best interaction with the production of white oyster mushrooms is a combination of 20% bran composition and 19 cm baglog size.  

scholarly journals Cultivation of Pleurotus sajor-caju on banana stalk and Bahia grass based substrates

2011 ◽  
Vol 29 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Félix G de Siqueira ◽  
Emerson T Martos ◽  
Romildo da Silva ◽  
Eustáquio S Dias
Keyword(s):  
Nitrogen Concentration ◽  
Biological Efficiency ◽  
Textile Mill ◽  
Physicochemical Factors ◽  
Nitrogen Concentrations ◽  
Pleurotus Sajor Caju ◽  
Bahia Grass ◽  
High Concentrations ◽  
Cultivation Substrate ◽  
Protein Rich

Banana stalks and Bahia grass were utilized as basic starting materials for the production of the mushroom Pleurotus sajor-caju. Banana stalks were combined with other waste or supplement products (wheat bran, coast-cross hay, bean straw and cotton textile mill) to obtain different nitrogen concentrations. Since Bahia grass is relatively rich in protein, it was combined with other substrates (banana stalk, coast-cross hay and bean straw) to maintain a substrate nitrogen concentration of about 1.5%. Banana stalks and Bahia grass were both more efficient in the production of the mushroom P. sajor-caju when utilized without the addition of other substrates, with biological efficiencies of 74.4% and 74.12%, respectively. When combined with other substrates or grasses, there was a drop in biological efficiency, independent of the concentration of nitrogen. Furthermore, the addition of protein-rich waste to banana stalks resulted in a decrease or absence of fructification, which indicates that high concentrations of nitrogen in the cultivation substrate may hinder the cultivation of this mushroom. On the other hand, results reveal that the ideal concentration of nitrogen may depend on other physicochemical factors and these factors may determine the success in cultivating P. sajor-caju. Therefore, we conclude that P. sajor-caju may be cultivated on banana stalk and Bahia grass as pure substrates, not being necessary their supplementation or combine them with another substrates.

scholarly journals ASPEK LINGKUNGAN SEBAGAI FAKTOR PENENTU KEBERHASILAN BUDIDAYA JAMUR TIRAM (PLEUROTUS SP)

2011 ◽  
Vol 9 (3) ◽  
Author(s):  
Netty Widyastuti
Keyword(s):  
Relative Humidity ◽  
Light Intensity ◽  
Fruiting Body ◽  
Oyster Mushroom ◽  
Biological Efficiency ◽  
Efficiency Ratio

Environment aspect as requirement growing of oyster mushroom (Pleurotus sp.), give priority like temperature, relative humidity, time, CO2 and light. The parameter have different influence to each stadium or stage, for example mycelia growing on substrate, formation of fruiting primordia, formation of fruiting body, harvest cycle and BER (Biological Efficiency Ratio) value. pH medium have to regulated (betweenN6-7), incubation phase (22 - 28 O C ), humidity 60-70% and fruiting body phase 16 -N22 O C. Growing mycelium without light, growing fruiting body is needed light stimulant, light intensity 60 - 70 %.

Cultivation and Harvesting of Selenium-Enriched Ganoderma lingzhi and Spent Medium Using Kudzu Vine as Substrate

2019 ◽  
Vol 18 (2) ◽  
pp. 152-157
Author(s):  
Zeng Xianlu ◽  
Han Fei ◽  
Zhong Yanmei
Keyword(s):  
Growth Medium ◽  
Wheat Bran ◽  
Fruiting Body ◽  
Sodium Selenite ◽  
Human Consumption ◽  
Growth Substrate ◽  
Organic Selenium ◽  
Significant Difference ◽  
Ganoderma Lingzhi ◽  
Run Speed

In order to harvest selenium-enriched fruiting body and spores of Ganoderma lingzhi and spent medium, G. lingzhi was cultivated in kudzu vine as substrate and the bio-transformation of selenite was evaluated. The growth medium consisted of Kudzu vine supplemented with 20% wheat bran or sawdust or none. The growth medium was supplemented with 0, 10, 20, 30, and 50 mg/kg of sodium selenite. We found a significant difference in spawn run speed, fruiting body and spore yields when Kudzu vine was supplemented with wheat bran or sawdust. However, when whole-kudzu vine was used alone as substrate, it resulted in a significantly lower spawn run speed, fruiting body, and spore yields compared with kudzu vine + sawdust substrate and kudzu vine + wheat bran substrate. The selenium content in fruiting body and spores increased with increasing sodium selenite supplementation and approximately equaled half of the selenium in the substrate. No selenite was detected in both the fruiting body and spores. However, in the spent medium when sodium selenite was supplemented at 10, 20, 30, 50 mg/kg, the residual selenite concentration decreased to 0.45, 0.72, 1.29, and 1.95 mg/kg, respectively, suggesting a higher selenite transformation (92.27–93.57%). In conclusion, if Ganoderma fruiting body and spores were to be harvested for human consumption, approximately 50 mg/kg selenite should be added to the growth substrate. On the other hand, if the spent medium was to be used as an organic selenium source, the optimal sodium selenite supplementation level would be 10 mg/kg.

scholarly journals Determination of Antioxidant Parameters of Pleurotus Mushrooms Growing on Different Wood Substrates

2017 ◽  
Vol 61 (4) ◽  
pp. 53-58
Author(s):  
I. Strapáč ◽  
M. Kuruc ◽  
M. Baranová
Keyword(s):  
Oyster Mushroom ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Fruiting Bodies ◽  
Pleurotus Pulmonarius ◽  
Antioxidant Parameters ◽  
Oyster Mushrooms ◽  
Maximum Content ◽  
Β Carotene

AbstractExtracts of the fruiting bodies of the Oyster mushroom (Pleurotus ostreatus) grown on wood substrates (beech, oak, linden, walnut, poplar) and extracts of the fruiting bodies of the Oyster mushroom (Pleurotus pulmonarius) grown in nature on aspen wood were used to determine the total phenols, total flavonoids, lycopene and β-carotene. The content of individual antioxidants varies considerably depending, not only on the substrate, but also on the extracting agents. The highest content of total phenols and total flavonoids was found in methanol and water extracts of the fruiting bodies of the Oyster mushrooms grown on oak and linden substrates. The maximum content of lycopene and β-carotene was determined in acetone and n-hexane (ratio 4 : 6) extracts of the fruiting bodies of the Oyster mushroom grown on an oak block. The results obtained in this study demonstrated that the quantitative and also probably the qualitative composition of the antioxidants in the fruiting bodies of Oyster mushrooms depended considerably on the substrate composition.

scholarly journals Selenium speciation and biological characteristics of selenium-rich Bailing mushroom, Pleurotus tuoliensis

2018 ◽  
pp. 704
Author(s):  
Yajie Zou, Fang Du, Haijun Zhang, Qingxiu Hu
Keyword(s):  
Fruiting Body ◽  
Sodium Selenite ◽  
Selenium Concentration ◽  
Biological Characteristics ◽  
Selenium Speciation ◽  
Fruiting Bodies ◽  
Good Tolerance ◽  
Icp Ms ◽  
Mycelia Growth

Nowadays the study of selenium-rich mushrooms is very popular. In the present study, selenium speciation in fruiting body of Pleurotus tuoliensis was investigated in cultivation substrates with different concentrations of sodium selenite, as well as mycelia growth and mushroom development. The results showed that the P. tuoliensis mycelia appeared good tolerance to selenium at all test concentrations. A selenium concentration of 10 mg/kg promoted fruiting of P. tuoliensis; the fruiting bodies were of good quality and had a low malformation rate. HPLC–ICP-MS determined that organic seleniums enriched in stipes and caps existed mainly in the form of selenoCystine and selenoMethionine at selenium concentrations of 10-100 mg/kg. These findings suggest that P. tuoliensis could be developed as a selenium-rich mushroom product for use as a novel dietary source of bioavailable supplemental selenium.

scholarly journals Strain improvement of Escherichia coli K-12 for recombinant production of deuterated proteins

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vinardas Kelpšas ◽  
Claes von Wachenfeldt
Keyword(s):  
Escherichia Coli ◽  
Structural Biology ◽  
Strain Improvement ◽  
Growth Media ◽  
Genomic Changes ◽  
E Coli ◽  
Recombinant Production ◽  
High Concentrations ◽  
Neutron Protein Crystallography ◽  
K 12

AbstractDeuterium isotope labelling is important for structural biology methods such as neutron protein crystallography, nuclear magnetic resonance and small angle neutron scattering studies of proteins. Deuterium is a natural low abundance stable hydrogen isotope that in high concentrations negatively affect growth of cells. The generation time for Escherichia coli K-12 in deuterated medium is substantially increased compared to cells grown in hydrogenated (protiated) medium. By using a mutagenesis plasmid based approach we have isolated an E. coli strain derived from E. coli K-12 substrain MG1655 that show increased fitness in deuterium based growth media, without general adaptation to media components. By whole-genome sequencing we identified the genomic changes in the obtained strain and show that it can be used for recombinant production of perdeuterated proteins in amounts typically needed for structural biology studies.

scholarly journals Comparison of Three Deoxidation Agents for Ozonated Broths Used in Anaerobic Biotechnological Processes

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 65
Author(s):  
Ewelina Pawlikowska ◽  
Jaroslaw Domanski ◽  
Piotr Dziugan ◽  
Joanna Berlowska ◽  
Weronika Cieciura-Wloch ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Process Conditions ◽  
Growth Media ◽  
Nutrient Supplementation ◽  
Yeast Biomass ◽  
Residual Oxygen ◽  
The Media ◽  
Biotechnological Processes ◽  
The Subject ◽  
High Concentrations

Anaerobic fermentation of organic compounds is used in many biotechnological processes and has been the subject of much research. A variety of process conditions and different growth media can be used to obtain microbial metabolites. The media must be free from contamination before fermentation. Sterilization is most often achieved by applying heat or other treatments, such as ozonation. Sterilization of liquid media using ozone can be very beneficial, but this method introduces high concentrations of residual oxygen, which inhibit anaerobic processes. Deoxidation is therefore necessary to remove the oxygen from ozonated broths. This study evaluates the effectiveness of three deoxidation agents for two kinds of fermentation media based on malt or molasses: ultrasound, iron(II) sulfate, and Metschnikowia sp. yeast. The time needed for deoxidation varied, depending on the kind of broth and the deoxidation agent. In general, the dynamics of oxygen removal were faster in malt broth. A comparative analysis showed that yeast biomass was the most effective agent, achieving deoxidation in the shortest time. Moreover, the fully deoxidated broth was supplemented with yeast biomass, which is rich in biogenic substrates, expressed as a protein content of 0.13–0.73 g/L. Application of Metschnikowia sp. may therefore be considered as an effective strategy for simultaneous deoxidation and nutrient supplementation of broths used in anaerobic biotechnological processes.

scholarly journals Evaluation of Using Spent Mushroom Sawdust Wastes for Cultivation of Auricularia polytricha

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1892
Author(s):  
Chiu-Yeh Wu ◽  
Chih-Hung Liang ◽  
Zeng-Chin Liang
Keyword(s):  
Fruiting Body ◽  
Pleurotus Ostreatus ◽  
Pleurotus Eryngii ◽  
Biological Efficiency ◽  
Main Ingredient ◽  
Auricularia Polytricha ◽  
Pleurotus Cystidiosus

The purpose of this study was to investigate the suitability of different spent mushroom sawdust wastes (SMSWs) and different proportions of SMSWs as potential substrates for the cultivation of Auricularia polytricha by evaluating yield and biological efficiency of the fruiting body. Nine SMSWs were respectively utilized as the main ingredient in the cultivation of A. polytricha. Then, spent Pleurotus eryngii, Pleurotus cystidiosus, and Pleurotus ostreatus sawdust wastes were screened among these nine SMSWs to be utilized as substrate and to determine the suitable proportion of SMSW in the cultivation of A. polytricha based on their yields and biological efficiencies. The highest yield and biological efficiency (total of two flushes) of A. polytricha cultivation on a single SMSW substrate was obtained with spent P. eryngii sawdust waste, followed by spent P. cystidiosus and P. ostreatus sawdust wastes. These three SMSWs were then applied in nine combination substrates, which were screened based on yield and biological efficiency for cultivation of A. polytricha. The combination substrate with the highest yield and biological efficiency of A. polytricha cultivation was P. eryngii (PES) + P. cystidiosus spent sawdust (PCYS) (235.4 g/bag yield and 58.85% biological efficiency); its yield was 1.62 folds higher than that of the control. From the results, we found that it was feasible to use spent sawdust wastes of P. eryngii and P. cystidiosus to replace sawdust for cultivation of A. polytricha.

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