Effect of various culture media on mycelium growth andSporulation of dermatophytes isolated from the Patients of sms hospital,jaipur, rajasthan

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
SACHIN KUMAR ◽  
SEEMA BHADAURIA
Keyword(s):  
Culture Media ◽  
Mycelium Growth

scholarly journals Effect of Nutritive Media and PH on in vitro Mycelial Growth of some Pleurotus eryngii Strains

2016 ◽  
Vol 73 (2) ◽  
pp. 276
Author(s):  
Alexandru Valentin Zagrean ◽  
Gicuta Sbirciog ◽  
Mihaela Alina Buzatu ◽  
Iuliana Mandru
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Weak Acid ◽  
Pleurotus Eryngii ◽  
Mycelium Growth ◽  
Ph Values ◽  
Mycelial Growth Rate ◽  
Initial Ph ◽  
Optimal Values

The mycelial growth rate of 4 strains of Pleurotus eryngii was first studied on different culture media - MEA, PDA and WEA (wheat extract agar). In the next experiment, we checked the mycelial growth on the same medium but at 3 different pHs. MEA medium ensured the highest growth rates for all the strains under test, followed by PDA. Pery-G strain showed the fastest growing rate on MEA (5.79 mm day-1) and PDA (3.04 mm day-1), respectively. Test concerning the mycelium growth on PDA at different initial pH values (5.5, 6.0, 6.5) showed the preference of our P.eryngii strains for media with weak acid to the neutral reaction , the optimal values being 6.0. There were no big differences among mycelium growing rates of the same strain for the three pH variants

scholarly journals The effect of cultivation conditions on growth, sporulation and formation of morphological structures of Topospora myrtilli (Feltg.) boerema

2012 ◽  
Vol 61 (2) ◽  
pp. 167-174
Author(s):  
Mariusz Szmagara ◽  
Ewa Zalewska
Keyword(s):  
Growth And Development ◽  
Linear Growth ◽  
Culture Media ◽  
The Other ◽  
Optimal Temperature ◽  
Cultivation Conditions ◽  
Mycelium Growth ◽  
Wide Range ◽  
Microscopic Features ◽  
Intensive Growth

The growth of <i>Topospora myrtilli</i> isolates, obtained in the years 2001-2003, was studied on PDA medium at -10, +2, +6, +12, +18, +22 and +28°C. The growth and sporulation of the isolates were observed at +22°C on the following culture media: PDA, maltose - MA and mineral - MSA, as well as on two versions of oat media, one containing 20 g of oat flakes per litre (OA-20), and the other containing 50 g of oat flakes per litre (OA-50). The observations of the linear growth of colonies of the studied strains were carried out during a period of 21 days, whereas the formation of morphological structures until the 52<sup>nd</sup> day of cultivation. It was found that <i>T. myrtilli</i> can grow and sporulate in a wide range of temperature, even at +2°C. The pathogen growth activates with the increase of temperature, up to the optimal temperature, i.e. from +18°C to +22°C. The increase of temperature to +28°C did not favour the mycelium growth and prevents pathogen sporulation. PDA, OA-20, OA- 50 and MA media can be considered to be the most suitable for the growth and development of <i>T. myrtilli</i>, due to the intensive growth and formation of typical macro- and microscopic features. It is necessary to emphasise the great usefulness of oat medium, especially OA-50, for the cultivation of <i>Topospora myrtilli</i>, in the aspect of sporulation of the fungus. It was found that mineral medium is unsuitable due to the formation of mycelium with an untypical structure and colouration as well as late conidial sporulation.

scholarly journals EVALUATION THE EFFICINCY OF SUBSTRATE IN YIELD CHARACTERISTICS AND QUALITIES OF Flammulina velutipes

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Abdulrazzaq & et al.
Keyword(s):  
Wheat Straw ◽  
Wheat Bran ◽  
Culture Media ◽  
Quality Characteristics ◽  
Flammulina Velutipes ◽  
Biological Efficiency ◽  
Fungal Mycelium ◽  
Mycelium Growth ◽  
The Mean ◽  
Palm Waste

This study was conducted to evaluate the efficiency of the substrate on the productivity and quality for Flammulina velutipes. Results of the effect of different agro-waste sawdust (SD), wheat straw (WS) and palm waste (PW). on the mean fungal mycelium growth shows that Flammulina velutipes gave the highest mean fungal mycelium growth on SD culture media amended with 2, 3, 4% lime at 30c. evaluation the efficiency of the above  agro-waste  substrate morphological and productivity showed that, the shortest period for the full growth of F. velutipes mycelium was on SD substrate, the shortest period for the pinhead formation 8.6days on  the fungus gave highest yield on WS which was 152.3 gm/ bag with 61.1% biological efficiency. The shortest time for full growth of F. velutipes was achieved in PW amended with wheat bran, the highest yield was in WS enriched with wheat bran which 306.7 gm/ bag with 129.3% biological efficiency. Results showed differences in quality characteristics in fruit bodies  of fungi by effect of substrate. 

scholarly journals INFLUENCE OF MAGNESIUM (Mg) SOURCE ON THE Cordyceps militaris (L.) MUSHROOM MYCELIUM GROWTH

2021 ◽  
Vol 10 (19) ◽  
pp. 333-340
Author(s):  
Melinda Rózsa ◽  
Dănuț-Nicolae Măniuțiu ◽  
Emese Egyed
Keyword(s):  
Magnesium Sulphate ◽  
Culture Medium ◽  
Culture Media ◽  
Magnesium Carbonate ◽  
Cordyceps Militaris ◽  
Optimal Concentration ◽  
Fungal Mycelium ◽  
Special Importance ◽  
Mycelium Growth ◽  
Oxidation Processes

Magnesium (Mg) is an element with a role in oxidation processes. The optimal concentration of magnesium for the development of fungi is associated with an optimal concentration of phosphorus (P), which through its essential constituents, is an important element for the existence of biological systems in nature through nucleic acids, phospholipids, phosphoglycerides, phytin and phosphates. Magnesium has a role in the activity of certain enzymes and in respiration, being a component of protein substances with a special importance for microorganisms being included in reducing and phosphorylating enzymes and for protein synthesis. In this study, magnesium sulphate (MgSO4), which is an accessible source of both magnesium and sulphur, as well as magnesium carbonate (MgCO3) were used as sources of magnesium, both being used in different concentrations in the growth medium. The culture medium or substrate influences the growth of the fungal mycelium, through the mineral, nutritive and stimulating substances in their composition. Using experimental culture media, an isolated tissue culture was performed from 2 strains of Cordyceps militaris (L.) fungus. The culture was performed in Petri dishes, incubated at a temperature of 24 ° C, aiming to increase the mycelium for 15 days. The highest increase was recorded by strain CI 32 with the addition of magnesium sulphate.

scholarly journals European apple canker: morphophysiological variability and pathogenicity in isolates of Neonectria ditissima in southern Brazil

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Jonatas da Silva Campos ◽  
Amauri Bogo ◽  
Rosa Maria Valdebenito Sanhueza ◽  
Ricardo Trezzi Casa ◽  
Fabio Nascimento da Silva ◽  
...  
Keyword(s):  
Geographical Origin ◽  
Culture Media ◽  
Growth Index ◽  
Southern Brazil ◽  
Mycelium Growth ◽  
Disease Symptoms ◽  
Geographical Regions ◽  
Colony Color ◽  
Specific Pair ◽  
Apple Canker

ABSTRACT: European apple canker (EC) is caused by Neonectria ditissima, a pathogen officially registered as a quarantine pest in 2012. Thirty-five isolates of N. ditissima of different geographical regions of southern Brazil from apple branches showing symptoms of EC were identified by the specific pair primers Ch1 and Ch2 and analyzed concerning the virulence on Gala apple cultivar and morphophysiological characteristics. The disease symptoms were characterized and the isolates compared based on average mycelium growth (AMG), mycelium growth index (MGI), colony color, conidia type, dimensions and growth on potato dextrose agar (PDA), malt agar (AM), and synthetic SNAY (SN) culture media. Nineteen isolates showed the greatest AMG on PDA, forming three growth groups of 35.56 (GI), 52.71 (GII), and 62.67mm (GIII). Seven isolates showed MGI greater than 4.0mm diameter on PDA compared with that on AM and SN. The highest conidia production was on SN, and the predominant colony color in all media was white to beige with central pigmentation of brown and borders colored in shades of beige. There were significant differences among the average dimensions of micro- and macroconidia on PDA, AM, and SN. The pathogenicity was confirmed for all isolates despite of different morphophysiological characteristics. There was no correlation among isolates morphophysiological variability, virulence, and geographical origin.

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Inhibition of Pro-Inflammatory Cytokine Secretion by Select Antioxidants in Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 90 (1-2) ◽  
pp. 103-112 ◽  
Author(s):  
Michael J. Haas ◽  
Marilu Jurado-Flores ◽  
Ramadan Hammoud ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Coronary Artery ◽  
Inflammatory Cytokines ◽  
Inflammatory Cytokine ◽  
Culture Media ◽  
Cytokine Secretion ◽  
Human Coronary Artery ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract. Inflammatory and oxidative stress in endothelial cells are implicated in the pathogenesis of premature atherosclerosis in diabetes. To determine whether high-dextrose concentrations induce the expression of pro-inflammatory cytokines, human coronary artery endothelial cells (HCAEC) were exposed to either 5.5 or 27.5 mM dextrose for 24-hours and interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF α) levels were measured by enzyme immunoassays. To determine the effect of antioxidants on inflammatory cytokine secretion, cells were also treated with α-tocopherol, ascorbic acid, and the glutathione peroxidase mimetic ebselen. Only the concentration of IL-1β in culture media from cells exposed to 27.5 mM dextrose increased relative to cells maintained in 5.5 mM dextrose. Treatment with α-tocopherol (10, 100, and 1,000 μM) and ascorbic acid (15, 150, and 1,500 μM) at the same time that the dextrose was added reduced IL-1β, IL-6, and IL-8 levels in culture media from cells maintained at 5.5 mM dextrose but had no effect on IL-1β, IL-6, and IL-8 levels in cells exposed to 27.5 mM dextrose. However, ebselen treatment reduced IL-1β, IL-6, and IL-8 levels in cells maintained in either 5.5 or 27.5 mM dextrose. IL-2 and TNF α concentrations in culture media were below the limit of detection under all experimental conditions studied suggesting that these cells may not synthesize detectable quantities of these cytokines. These results suggest that dextrose at certain concentrations may increase IL-1β levels and that antioxidants have differential effects on suppressing the secretion of pro-inflammatory cytokines in HCAEC.

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