scholarly journals Malondialdehyde Meningkatkan Tingkat Antigen Khusus Prostat di Antara Pekerja Bengkel Mobil

2021 ◽  
Vol 49 (1) ◽  
pp. 57-62
Author(s):  
Nendyah Roestijawati ◽  
Yudhi Wibowo
Keyword(s):  
Lipid Peroxidation ◽  
Specific Antigen ◽  
Oxygen Species ◽  
Elisa Method ◽  
Cross Sectional ◽  
The Mean ◽  
Auto Body ◽  
Cross Sectional Design ◽  
Cd Exposure

Cadmium (Cd) is one of the environmental factors that can cause prostate cancer. Cadmium can induces metallothionein (MT) in several tissues that functions to carry out an intracellular detoxification procession by binding to Cd. Exposure of Cd can cause an increase in reactive oxygen species which will cause lipid peroxidation with the end product being malondialdehyde (MDA). The research aims to determine the correlation between MT, MDA and prostate specific antigen (PSA) among auto body repair workers. The study used a cross sectional design involving 32 auto body repair workers and their ages ranged from 18 to 66 years. Measurement of MT, MDA and PSA using the ELISA method. We performed a multiple regression analysis to evaluate the relationships between MT, MDA with PSA for age and other covariates. The mean age of subjects was 38.88 ± 12.82 years. The mean levels of MT was 2.84 ± 1.18 ng/dL, MDA was 8.22 ± 20.06 ng / dL and PSA was 0.86 ± 1.79 ng / dL. The study found that MT (β = 0,232; 95% C.I. 0,202 – 0,503; p= <0,001) and MDA (β = 0.827; 95% CI. 0.065 – 0,083; p=<0,001) significantly predicted PSA. MDA (β = 0.827) has stronger relationship with PSA compared to MT (β =0.232). This results confirmed the role of MT and MDA on PSA synthesis. Metallothionein induce the process of lipid peroxidation with the end product being malondialdehyde (MDA) and increase PSA. Keywords : auto body repair workers, metallothionein, malondialdehyde, prostate Abstrak Kadmium (Cd) merupakan salah satu faktor lingkungan yang dapat menyebabkan kanker porstat. Kadmium dapat menginduksi metalotionin (MT) di beberapa jaringan yang akan berikatan dengan Cd untuk proses detoksifikasi. Pajanan Cd dapat meningkatkan ROS yang akan meyebabkan terjadinya peroksidase lipid dengan hasil akhir malondialdehid (MDA). Penelitian bertujuan untuk mengetahui hubungan antara MT, MDA dan PSA pada pekerja bengkel mobil. Penelitian menggunakan disain kros seksional dengan subyek sebanyak 32 pekerja bengkel mobil yang berusia 18 hingga 66 tahun. Pengukuran MT, MDA dan PSA menggunakan metode ELISA. Analisis statistik menggunakan analisis regresi berganda. Rata-rata usia subyek adalah 38.88 ± 12.82 tahun. Rata-rata kadar MT 2.84 ± 1.18 ng/dL, MDA 8.22 ± 20.06 ng / dL dan PSA 0.86 ± 1.79 ng / dL. Hasil uji didapatkan MT (β = 0,232; 95% C.I. 0,202 – 0,503; p= <0,001) dan MDA (β = 0.827; 95% CI. 0.065 – 0,083; p=<0,001) berpengaruh terhadap PSA secara signifikan. MDA (β = 0.827) mempunyai hubungan dengan PSA yang lebih kuat dibanding MT (β =0.232). Hasil penelitian mengkonfirmasi adanya peran MT dan MDA pada sintesis PSA. MT menginduksi proses peroksidase lipid dengan produk akhir MDA dan meningkatkan kadar PSA. Kata kunci : pekerja bengkel mobil, metalotionin, malondialdehid, prostat

scholarly journals Malondialdehyde Meningkatkan Tingkat Antigen Khusus Prostat di Antara Pekerja Bengkel Mobil

2021 ◽  
Vol 49 (1) ◽  
pp. 57-62
Author(s):  
Nendyah Roestijawati ◽  
Yudhi Wibowo
Keyword(s):  
Lipid Peroxidation ◽  
Specific Antigen ◽  
Oxygen Species ◽  
Elisa Method ◽  
Cross Sectional ◽  
The Mean ◽  
Auto Body ◽  
Cross Sectional Design ◽  
Cd Exposure

Cadmium (Cd) is one of the environmental factors that can cause prostate cancer. Cadmium can induces metallothionein (MT) in several tissues that functions to carry out an intracellular detoxification procession by binding to Cd. Exposure of Cd can cause an increase in reactive oxygen species which will cause lipid peroxidation with the end product being malondialdehyde (MDA). The research aims to determine the correlation between MT, MDA and prostate specific antigen (PSA) among auto body repair workers. The study used a cross sectional design involving 32 auto body repair workers and their ages ranged from 18 to 66 years. Measurement of MT, MDA and PSA using the ELISA method. We performed a multiple regression analysis to evaluate the relationships between MT, MDA with PSA for age and other covariates. The mean age of subjects was 38.88 ± 12.82 years. The mean levels of MT was 2.84 ± 1.18 ng/dL, MDA was 8.22 ± 20.06 ng / dL and PSA was 0.86 ± 1.79 ng / dL. The study found that MT (β = 0,232; 95% C.I. 0,202 – 0,503; p= <0,001) and MDA (β = 0.827; 95% CI. 0.065 – 0,083; p=<0,001) significantly predicted PSA. MDA (β = 0.827) has stronger relationship with PSA compared to MT (β =0.232). This results confirmed the role of MT and MDA on PSA synthesis. Metallothionein induce the process of lipid peroxidation with the end product being malondialdehyde (MDA) and increase PSA. Keywords : auto body repair workers, metallothionein, malondialdehyde, prostate Abstrak Kadmium (Cd) merupakan salah satu faktor lingkungan yang dapat menyebabkan kanker porstat. Kadmium dapat menginduksi metalotionin (MT) di beberapa jaringan yang akan berikatan dengan Cd untuk proses detoksifikasi. Pajanan Cd dapat meningkatkan ROS yang akan meyebabkan terjadinya peroksidase lipid dengan hasil akhir malondialdehid (MDA). Penelitian bertujuan untuk mengetahui hubungan antara MT, MDA dan PSA pada pekerja bengkel mobil. Penelitian menggunakan disain kros seksional dengan subyek sebanyak 32 pekerja bengkel mobil yang berusia 18 hingga 66 tahun. Pengukuran MT, MDA dan PSA menggunakan metode ELISA. Analisis statistik menggunakan analisis regresi berganda. Rata-rata usia subyek adalah 38.88 ± 12.82 tahun. Rata-rata kadar MT 2.84 ± 1.18 ng/dL, MDA 8.22 ± 20.06 ng / dL dan PSA 0.86 ± 1.79 ng / dL. Hasil uji didapatkan MT (β = 0,232; 95% C.I. 0,202 – 0,503; p= <0,001) dan MDA (β = 0.827; 95% CI. 0.065 – 0,083; p=<0,001) berpengaruh terhadap PSA secara signifikan. MDA (β = 0.827) mempunyai hubungan dengan PSA yang lebih kuat dibanding MT (β =0.232). Hasil penelitian mengkonfirmasi adanya peran MT dan MDA pada sintesis PSA. MT menginduksi proses peroksidase lipid dengan produk akhir MDA dan meningkatkan kadar PSA. Kata kunci : pekerja bengkel mobil, metalotionin, malondialdehid, prostat

scholarly journals Levels of molecular angiogenic and antiangiogenic in pregnant women with risk of preeclampsia

2019 ◽  
Vol 8 (10) ◽  
pp. 4034
Author(s):  
Diah Wulandari ◽  
Mohamad Sulchan ◽  
Syarief Thaufik Hidayat
Keyword(s):  
Risk Factor ◽  
Pregnant Women ◽  
Molecular Level ◽  
Elisa Method ◽  
Cross Sectional ◽  
Primary Health ◽  
The Mean ◽  
Cross Sectional Design ◽  
Plgf Ratio ◽  
Risk Of Preeclampsia

Background: Angiogenic and antiangiogenic imbalances play a major role in the pathogenesis of preeclampsia. Increased production of sFlt-1 by the placenta causes free circulating PIGF and VEGF concentrations to lower because it is bound by sFlt-1. Measuring levels of angiogenic and antiangiogenic proteins as biomarkers indicates placental dysfunction and distinguishes preeclampsia from other disorders. This study aims to analyze the levels of angiogenic and antiangiogenic molecules in pregnant women at risk for preeclampsia.Methods: The study with a cross-sectional design was carried out in 11-15 weeks gestational age whom had a risk of preeclampsia with 30 samples in primary health care starting April-August 2018. Blood serum was measured by molecular levels of VEGF, PlGF, sFlt-1, and sFlt-1/PlGF ratio using the ELISA method. Data analysis used Pearson product moment test.Results: The mean of VEGF levels are 15.5±21.6, PlGF 89.7±55.5, sFlt-1 11519.4±5126.0 and the ratio sFlt-1/PlGF 166.7±102.1. Correlation value of risk factors for preeclampsia with molecular levels of VEGF r= -0.05; p = 0.76, PlGF r= -0.21; p = 0.26, sFlt r= 0.01; p =0.99 and ratio sFlt-1/PlGF r = 0.10; p = 0.58.Conclusions: The higher the total score of preeclampsia risk factor, the lower the molecular level of VEGF and PlGF is. Moreover, the higher the total score of preeclampsia risk factor, the higher the molecular level sFlt-1 and the sFlt-1/PlGF ratio is. There are no significant correlation between total score of preeclampsia risk factor and levels of molecule VEGF, PlGF, sFlt-1 and sFlt-1/PlGF ratio.

scholarly journals Perbedaan Kadar Malondialdehide dan Tromboksan B2 pada Remaja dengan Dismenore dan Tanpa Dismenore

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Berliana Irianti ◽  
Ermawati Ermawati ◽  
Arni Amir
Keyword(s):  
Lipid Peroxidation ◽  
Observational Study ◽  
Thiobarbituric Acid ◽  
Sample Analysis ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Mean ◽  
The Difference ◽  
Cross Sectional Design ◽  
Biomedical Laboratory

Abstrak Penyebab dismenore belum semuanya diketahui, ada dugaan peningkatan proses peroksida lipid yang akan mengaktivasi mediator inflamasi pada endometrium yang menimbulkan rasa nyeri haid (dismenore). Tujuan penelitian ini adalah menentukan perbedaan kadar malondialdehide dan tromboksan B 2  pada dismenore dan tanpa dismenore. Studi observasional ini menggunakan desain potong lintang komparatif. Subjek penelitian terdiri dari dua kelompok yaitu 23 remaja dismenore dan 23 remaja tanpa dismenore dengan waktu penelitian dari Juni sampai Juli 2014. Analisis sampel dilakukan di Laboratorium Biomedik dan Biokimia Universitas Andalas Padang. Pemeriksaan kadartromboksan B 2  menggunakan metode ELISA dan kadar malondialdehide menggunakan metode Asam Thiobarbiturat (TBA). Hasil penelitian diperoleh bahwa rerata kadar malondialdehid pada remaja dengan dismenore yaitu 2,60±0,63 µmol/ml, rerata remaja tanpa dismenore 1,98±0,12 µmol/ml dengan probabilitas p<0,05 (0,000), sedangkan reratakadar Tromboksan B 2  pada remaja dengan dismenore 20,043±9,56 ng/ml, rerata remaja tanpa dismenore 19,222±10,79 ng/ml, dengan probabilitas p>0,05 (0,786). Kesimpulan penelitian ini adalah terdapat perbedaan yang signifikan rerata kadar malondialdehid pada remaja dengan dismenore dan tanpa dismenore dan tidak terdapatperbedaan signifikan pada kadar tromboksan B 2 pada remaja dengan dismenore dan tanpa dismenore.Kata kunci: remaja, dismenore, malondialdehide, tromboksan B2 Abstract The precise cause of dysmenorrhea is still unclear, there may be increased lipid peroxidation process will activate the inflammatory mediators at endometrium that cause menstrual cramps (dysmenorrhea). The objective of this study was to determine the difference of malondialdehyde levels and thromboxane B 2  levels in dysmenorrhea and without dysmenorrhea. It was an observational study with comparative cross-sectional design. The subjects consisted of two groups, they are 23 adolescent with dysmenorrhea and 23 adolescents without dysmenorrhea, done in Juny -July 2014. Sample analysis was conducted in Laboratory of Biochemistry and Biomedical Laboratory of Andalas University Padang. The examination of Thromboxane B 2  levels used ELISA and the examination of malondialdehyde levels used a Thiobarbituric acid method. The results showed the mean of malondialdehyde levels in adolescents withdysmenorrhea was 2.60±0.63 µmol/ml, the mean level in adolescent without dysmenorrhea was 1.98±0.12 µmol/ml with probability p<0.05 (0.000), while the mean levels of thromboxane B 2  in adolescents with dysmenorrhea was 20.043±9.56 ng/ml, the mean level in adolescent without dysmenorrhea was 19.222±10.79 ng/ml, with probabilityp>0.05 (0.786). It can be concluded that there is a significant difference in the mean of malondialdehyde levels between adolescents with dysmenorrhea and without dysmenorrhea and there is no significant differences in thromboxane B 2 level between adolescents with dysmenorrhea and without dysmenorrheaKeywords: adolescent, dysmenorrhea, malondialdehyde, thromboxane B2

Person–job fit and job involvement: the curvilinear effect and the moderating role of goal orientation

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Dong Ju ◽  
Jingjing Yao ◽  
Li Ma
Keyword(s):  
Goal Orientation ◽  
Job Involvement ◽  
Job Fit ◽  
Performance Goal ◽  
Cross Sectional ◽  
Content Type ◽  
Learning Goal Orientation ◽  
Cross Sectional Design ◽  
Employee Needs

PurposeJob involvement is an important predictor of how well employees perform and feel at work. However, despite fruitful findings, little is known about how person–job (P–J) fit affects job involvement.Design/methodology/approachThis study used a cross-sectional design and collected data from 375 employees and 50 managers. Multivariate regression was applied to test the moderated curvilinear model.FindingsThis study found an inverted U-shaped relationship between P–J fit and job involvement. For employees with a strong performance goal orientation, maximum job involvement occurred at a higher level of P–J fit, whereas for employees with a strong learning goal orientation, maximum job involvement occurred at a moderate level of P–J fit.Practical implicationsManagers should be aware that solely maximizing fit may not constantly yield positive outcomes, and that ignoring differences in employee needs and goals may be counterproductive.Originality/valueThe study challenges the conventional wisdom that a high P–J fit is always productive by showing that a high fit may sometimes jeopardize job involvement, particularly for certain employees.

scholarly journals Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

2012 ◽  
Vol 1 (1) ◽  
pp. 18
Author(s):  
Amrit Kaur Bansal ◽  
Ranjna Sundhey Cheema ◽  
Vinod Kumar Gandotra
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Acrosome Reaction ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species ◽  
Sperm Capacitation ◽  
Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

scholarly journals ROLE OF IMMATURE/TOTAL NEUTROPHIL RATIO, LEUKOCYTE COUNT AND PROCALCITONIN IN DIAGNOSING NEONATAL SEPSIS

2018 ◽  
Vol 24 (2) ◽  
pp. 169
Author(s):  
I A Putri Wirawati1 ◽  
Aryati Aryati ◽  
A A Wiradewi Lestari
Keyword(s):  
Likelihood Ratio ◽  
Neonatal Sepsis ◽  
Leukocyte Count ◽  
Systemic Disease ◽  
Clinical Syndrome ◽  
Cross Sectional ◽  
Bacteria Growth ◽  
Long Time ◽  
Cross Sectional Design

Neonatal sepsis is a clinical syndrome of systemic disease, accompanied by bacteremia that occurs during infants in the first month of life. A late diagnosis might increase mortality. The presence of bacteria growth in blood cultures is a definitive diagnosis. Unfortunately, culture results are usually obtained of a long time. The study aimed to analyze sensitivity and specificity of the manual I/T ratio, automatic I/T ratio, leukocyte count and procalcitonin (PCT) to diagnose neonatal sepsis. This study used a cross-sectional design, from the NICU room in Sanglah General Hospital, Denpasar. There were 59 patients who met the study criteria. Along with blood culture as the gold standard in determining diagnosis of sepsis and with I/T ratio cut-off of 0.2, the sensitivity of manual I/T ratio was 69.2%, specificity 83.9%, PPV 63.9%, NPV 87% and likelihood ratio was 3.06. While the sensitivity of automatic I/T ratio was 47.6%, specificity 85.8%, PPV 55.1%, NPV 81.4% and likelihood ratio was 2.25. Based on the normal range of leukocyte count (9.1 - 34 x 103/μl), sensitivity of leukocyte count was 59%, specificity 71.5%, PPV 46.7%, NPV 80.9% and likelihood ratio was 1.59. With PCT cut-off 0.5 ng/mL, the obtained sensitivity of PCT was 64.3%, specificity 85.8%, PPV 64.3%, NPV 85.8% and likelihood ratio was 3.13. 

scholarly journals Public Perception of the Role of the Nigeria Police Force in Curbing the Menace of Kidnapping in Benin Metropolis, Southern Nigeria: A Criminological Study

2018 ◽  
Vol 7 (1) ◽  
pp. 65-72
Author(s):  
Barr. Emmanuel Imuetinyan Obarisiagbon ◽  
Mannie Omagie
Keyword(s):  
Public Perception ◽  
Police Force ◽  
Public Confidence ◽  
Cross Sectional ◽  
The Public ◽  
Southern Nigeria ◽  
Crime Detection ◽  
Cross Sectional Design ◽  
Problem Oriented Policing

Abstract Kidnapping for ransom has been on the increase in the last ten years in Nigeria and there appears to be no end in sight despite the existence of a police force whose statutory function of crime detection and prevention has come under fire for its abysmal performance. This study therefore examined the public perception of the role of the Nigeria police force in curbing the menace of kidnapping in Benin Metropolis, Southern Nigeria. This study adopted the problem-oriented policing theory in its explanation of the topic under investigation. It also employed the survey and cross-sectional design. The quantitative technique was utilized to collect data from the respondents while a total of 960 respondents were quantitatively sampled. Both descriptive and inferential statistics were used to analyse the quantitative data collected from the field. Findings from this investigation showed that there is a very poor public perception of the police and that there are a multiplicity of obstacles hindering the efforts of the police at curbing the activities of kidnappers in Nigeria. Based on the findings of the study, it was recommended that government should improve the funding of the police to boost the morale of the rank and file while the police on its part should get rid of the bad elements within its system in order for public confidence to be restored in its ability.

scholarly journals The Role of HLAC and NK Cells in Fetal Growth Restriction

2017 ◽  
pp. 142
Author(s):  
Sri Sulistyawati ◽  
Didon M Trimulya ◽  
Supriyadi H Respati ◽  
Soetrisno Soetrisno
Keyword(s):  
Nk Cells ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Nk Cell ◽  
Normal Pregnancy ◽  
Growth Restriction ◽  
Immunohistochemical Method ◽  
Cross Sectional ◽  
The Mean

Objective: To determine the role of HLA-C and NK cell expressions in fetal growth restriction (FGR). Methods: A cross sectional study design was used. This study was conducted at the Obstetrics and Gynecology Department of Dr. Moewardi General Hospital, Surakarta, its affiliated hospitals, and at the Pathological Anatomy Laboratory of the Faculty of Medicine, University of Sebelas Maret Surakarta. A total of 40 samples were included in this study. The samples consisted of 20 normal pregnancies and 20 pregnancies with FGR. HLA-C expression in the trophoblast and NK cells in decidua of the subjects who met the inclusion and exclusion criteria were examined using immunohistochemical method and statistical analysis with T test. Results: The mean expression of HLA-C in the trophoblast in the pregnant group with FGR was 9.021.30, normal pregnancy was 7.96 ± 0.97, p=0.01 (p<0.05). The mean expression of NK cells in decidua of pregnancy with FGR was 10.59 ± 2.11, normal pregnancy was 0.91 ± 8.18, with p=0.00 (p<0.05). Conclusion: The expressions of HLA-C in trophoblast and NK cells in decidua of pregnancy with FGR were higher compared with those of normal pregnancy. [Indones J Obstet Gynecol 2017; 5-3: 142-148] Keywords: fetal growth restriction, HLA-C, NK cells

THE ROLE HEALTH OFFICERS AS A DETERMINING FACTOR FOR THE SUCCESSFUL MEASLES RUBELLA IMMUNIZATION IN THE WORK AREA OF THE RAWANG HEALTH CENTER, PADANG CITY

2022 ◽  
Vol 7 (2) ◽  
pp. 150-160
Author(s):  
Putri Rahmadani ◽  
Besral Besral ◽  
Masrizal Masrizal
Keyword(s):  
Health Center ◽  
Health Workers ◽  
Immunization Coverage ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Research Population ◽  
Chi Square Test ◽  
Cross Sectional Design

Background: The measles rubella (MR) immunization coverage rate in Padang City is only 30.82%, still far below Indonesia's national target of 95%. The coverage of measles rubella immunization at the Rawang Health Center is around 62.1%. This study aims to determine the role of health workers in the success of measles rubella immunization in the Rawang Public Health Center, Padang City. Methods: This study used a cross sectional design. The study was conducted in October 2018-July 2019. The research population is mothers who have children aged 12-59 months (toddlers) as many as 1807 respondents. The sampling method is proportional random sampling with a sample of 87 respondent. Data was collected by interview using a questionnaire. Results: The results showed that 37.9% of children under five had not been immunized against measles rubella and 44.8% of health workers had an unfavorable role. The results of the chi-square test showed a significant relationship  between  the role of officers in  the success of measles rubella immunization  (p-value=0.020). Recommendation: It is hoped that health workers can increase health promotion activities regarding the risks due to children not being immunized against measles rubella and intensify door-to-door programs in the implementation of measles rubella immunization

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