scholarly journals Aktivitas Penghambatan α-glukosidase dan Peredaman Radikal Bebas Ekstrak Kapang Endofit yang Diisolasi dari Rimpang Kunyit

2019 ◽  
Vol 29 (3) ◽  
pp. 189-196
Author(s):  
Eris Septiana ◽  
Bustanussalam Bustanussalam ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Blood Sugar ◽  
Endophytic Fungi ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Ic50 Values ◽  
Sugar Levels

Abstract Diabetes mellitus is one of the metabolic disorders that causes an increase in blood sugar levels above normal the normal threshold. The case of diabetes is usually accompanied by an increase in free radicals in the patient’s body. In Indonesia, one of the plants traditionally used to treat high blood sugar levels and contains antioxidant compounds is turmeric. The use of endophytic fungi from medicinal plants as a source of active compounds is widely carried out. Therefore this research aims to determine the antidiabetic and antioxidant activity of the extract of turmeric endophytic fungi from Bogor in vitro. The antidiabetic test used the method of inhibiting of α-glucosidase enzymes, while the antioxidant test used the method of reducing free radicals 2,2-Diphenyl-1-picrylhydrazyl (DPPH). The test results show that the five endophytic mold ethyl acetate extracts have the ability to inhibit the α-glukosidase enzyme and antioxidant activity. Ethyl acetate extract isolate Bo.Ci.Cl.R5 was the most active in α-the the inhibitory activity of a glucosidase enzyme activity and free radical reduction with IC50 values of 336.22 μg/mL and 91.70 μg/mL respectively. Therefore extract of endophytic fungi isolates Bo.Ci.Cl.R5 isolate has the potential to be developed as an alternative raw material for antidiabetic drugs. Abstrak Diabetes melitus merupakan salah satu kerusakan metabolisme tubuh yang menyebabkan naiknya kadar gula dalam darah di atas ambang batas normal. Kasus diabetes biasanya diiringi oleh meningkatnya radikal bebas dalam tubuh penderita. Di Indonesia, salah satu tanaman yang secara tradisional digunakan untuk mengobati kadar gula darah yang tinggi dan mengandung senyawa antioksidan ialah tanaman kunyit. Pemanfaatan kapang endofit asal tanaman berkhasiat obat sebagai sumber senyawa aktif banyak dilakukan. Oleh karena itu penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan antioksidan dari ekstrak kapang endofit rimpang kunyit asal Bogor secara in vitro. Uji antidiabetes menggunakan metode penghambatan aktivitas enzim α-glukosidase, sedangkan uji antioksidan menggunakan metode peredaman radikal bebas 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Hasil pengujian menunjukkan bahwa kelima ekstrak etil asetat kapang endofit memiliki kemampuan dalam menghambat enzim α-glukosidase dan memiliki aktivitas antioksidan. Ekstrak etil asetat isolat Bo.Ci.Cl.R5 merupakan yang paling aktif pada uji aktivitas penghambatan aktivitas enzim α-glukosidase dan peredaman radikal bebas dengan nilai IC50 masing-masing uji sebesar 336,22 μg/mL dan 91,70 μg/mL. Oleh karena itu ekstrak isolat kapang endofit Bo.Ci.Cl.R5 berpotensi dikembangkan sebagai alternatif bahan baku obat antidiabetes.

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

scholarly journals Crystal Guava (Psidium guajava L. “Crystal”): Evaluation of In Vitro Antioxidant Capacities and Phytochemical Content

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Rika Hartati ◽  
Hashifah I. Nadifan ◽  
Irda Fidrianny
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Activity Index ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Positive Correlation ◽  
Phytochemical Content ◽  
Fruit Extracts

Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural antioxidants is guava. The goals of this research were to investigate the antioxidant activity of leaves and fruit of crystal guava by determining the value of the Antioxidant Activity Index (AAI) using DPPH, CUPRAC, and FRAP; evaluate the total phenolic content (TPC) and total flavonoid content (TFC); analyse the correlation between the TPC and TFC with AAI DPPH, CUPRAC, and FRAP, and analyse the correlation between the 3 methods. Extraction was performed by the reflux method using n-hexane, ethyl acetate, and ethanol. Determination of AAI DPPH, CUPRAC, FRAP, the TPC, and the TFC was performed by UV-visible spectrophotometry. The correlation of the TPC and TFC with AAI DPPH, CUPRAC, and FRAP and, also, the correlation of the 3 methods were investigated by Pearson’s method. The antioxidant activity of leaves and fruit extracts of crystal guava showed AAI DPPH in the range of 0.33–56.46, CUPRAC 0.20–7.31, and FRAP 1.65–59.89. The highest TPC was given by ethanol leaf extracts (49.55 ± 1.45 g GAE/100 g), while the highest TFC was for n-hexane leaf extracts (9.68 ± 0.210 g QE/100 g). The TPC of leaves extract had a significantly positive correlation with AAI DPPH, CUPRAC, and FRAP. AAI DPPH, AAI CUPRAC, and AAI FRAP of leaves and fruit extract of crystal guava showed a significantly positive correlation. In general, leaves extract had strong antioxidant activity by the three methods. For the highest antioxidant activity, ethanol was the best solvent for extraction leaves and ethyl acetate for extraction fruit of crystal guava. The TPC in leaves extract contributed to the antioxidant activity by DPPH, CUPRAC, and FRAP methods. The Antioxidant activity of leaves and fruit extracts of crystal guava was linear by the three methods.

scholarly journals ANTIOXIDANT ACTIVITY OF LEAF EXTRACT FROM SIDA SUBCORDATA SPAN

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Rahma Yulia ◽  
Dachriyanus Dachriyanus ◽  
Amri Bachtiar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Spectrophotometric Methods ◽  
Dpph Method ◽  
Extract Fraction

This Study has tested the antioxidant activity of the extracts from Sida subcordata Span. Testing is done with antioxidant activity using DPPH method provides. Testing of antioxidant activity in vitro with spectrophotometric methods based on absorbance measurements radical DPPH (1,1-diphenylpicrylhydrazyl). All the extracts Showed radical scavenging activity. The ethyl acetate and methanol extracts were the most Appropriate as sources of antioxidant compounds as shown by Reviews their inhibition percentage (IP) values. Percentage inhibitor to extract ethyl acetate and methanol extract respectively 76.16% and 79.28% at a concentration of 1 mg / ml to 0.05 mM DPPH. while the results of the testing activities of antioxidant compounds y pure ethyl acetate extract fraction showed a smaller percentage inhibitor that is equal to 30% at the same concentration. Keyword - Sida subcordata Span, Malvaceae, Antioxidant, Alkaloid

scholarly journals Aktivitas Antioksidan Hidrolisat Kolagen Kulit Ikan Nila (Oreochromis niloticus)

2020 ◽  
Vol 23 (3) ◽  
pp. 423-433
Author(s):  
Deny Tri Prastyo ◽  
Wini Trilaksani ◽  
Nurjanah
Keyword(s):  
Antioxidant Activity ◽  
Oreochromis Niloticus ◽  
Biological Activities ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Average Percentage ◽  
Skin Collagen

Tilapia skin is a by-product of fish processing which can be used as an alternative raw material for collagen and its hydrolyzate. Collagen hydrolyzate is known to have potential biological activities including antioxidants. This study aims to examine the antioxidant activity of collagen hydrolyzate from the skin of tilapia (Oreochromis niloticus) in vitro. This research was divided into three stages including collagen pretratment, collagen extraction and collagen hydrolysis. In the hydrolysis process papain enzymes are used with different concentrations (4,000 U/g, 6,000 U/g, 8,000 U/g samples) with hydrolysis time for 60, 120 and 180 minutes. The average yield value of tilapia skin collagen hydrolyzate was 15.17%. The hydrolysis time, the concentration of enzymes given and the interaction between the two factors significantly affected the value of the degree of hydrolysis and antioxidant activity of the hydrolyzed collagen of tilapia skin. Collagen hydrolyzed for 120 minutes by giving an enzyme concentration of 8,000 U had an average percentage of degree of hydrolysis of 33.94%, and the best IC50 value was 93.32 µg/mL, categorized as strong antioxidant compounds. The zeta potential value of the tilapia skin collagen hydrolyzate was -10.9 mV.

scholarly journals IN VITRO SCREENING FOR ANTICHOLINESTERASE AND ANTIOXIDANT ACTIVITY OF EXTRACT OF GARCINIA ATROVIRIDIS

2018 ◽  
Vol 11 (9) ◽  
pp. 208
Author(s):  
Chitra V ◽  
Narayanan J
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanolic Extract ◽  
The Elderly ◽  
Therapeutic Drug ◽  
Brain Disorder ◽  
Ic50 Values ◽  
Dpph Scavenging

Objective: Garcinia atroviridis belongs to the family Clusiaceae. The whole plant was dried, extracted to obtain a xanthone-rich extract. Phytochemical studies of extract showed the presence of phenols, alkaloids, steroids, resins, and saponins. Alzheimer’s disease (AD) the most common form of dementia, a brain disorder which has been affecting the elderly. The natural sources such as plants are an abundant source having various medicinal properties.Methods: In the present study, G. atroviridis has been selected to test for the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity by Ellman’s method. The antioxidant activity has been performed by 2,2’-diphenyl-1-picrylhydrazyl radical (DPPH) assay.Results: The results showed that the extract from G. atroviridis could inhibit the activity of AchE and BuChE. The percentage inhibition of the ethanolic extract for AChE was found to be 69.4%, and ethyl acetate extract was found to be 75.6%, respectively. The percentage inhibition of the ethanolic extract for BuChE was found to be 59.4%, and ethyl acetate extract was found to be 63.2%, respectively. The IC50 values for DPPH scavenging activity were significant when compared with the standard ascorbic acid.Conclusion: The plant extract with significant antioxidant and anticholinesterase property has shown its potential to be investigated further as a therapeutic drug for AD.

scholarly journals Penentuan Aktivitas Antioksidan dan Antidiabetes Ekstrak Daun Matoa (Pometia pinnata J.R. Forst. & G. Forst.) secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 132-141
Author(s):  
Lestyo Wulandari ◽  
Ari Satya Nugraha ◽  
Ulfa Aliyatul Himmah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Pearson Correlation ◽  
The Body ◽  
Antidiabetic Activity ◽  
Ic50 Values ◽  
High Antioxidant Activity ◽  
Ethyl Acetate Extracts

Matoa (Pometia pinnata J.R. Forst. & G. Forst.) Matoa (Pometia pinnata J.R. Forst. & G. Forst.) is one of the plants that is used as a traditional medicine for diabetes mellitus due to an imbalance between the amount of ROS and antioxidants in the body. Therefore, it was carried out in vitro to see the antioxidant and antidiabetic activity in matoa leaf extract. The extraction of matoa leaves was carried out using the ultrasonication method for 30 minutes with methanol, ethanol, and ethyl acetate as solvents. Antioxidant activity is release through DPPH free radical inhibition, through the antidiabetic potential released by inhibiting the work of the α-amylase enzyme. Phytochemical test results showed the presence of secondary metabolites in the form of flavonoids, polyphenols, tannins, alkaloids, and terpenoids. The results of the research on methanol, ethanol, and ethyl acetate extracts of matoa leaves showed high antioxidant activity with IC50 values of 6.416 ± 0.176 ppm, 8.622 ± 0.066 ppm, and 170.637 ± 4.441 ppm, respectively, but they were less potent than vitamin C as a comparison which is 1.646 ± 0.015 ppm. Inhibition of the α-amylase enzyme showed IC50 values of 91.037 ± 0.750 ppm, 105,166 ± 2,423 ppm, and 785,436 ± 11,740 ppm in each of the methanol, ethanol, and ethyl acetate extracts while the IC50 value of acarbose as a comparison was 23,479 ± 0.347 ppm. The statistical data analysis of Pearson correlation showed that it had a positive relationship between the antioxidant and antidiabetic activity of matoa leaf extract as seen from the R-value of 0.998. The higher antioxidant activity, so the higher potential for inhibition of α-amylase enzyme.

scholarly journals IN VITRO ANTI-WRINKLE AND TYROSINASE INHIBITORY ACTIVITIES OF GRAPEFRUIT PEEL and STRAWBERRY EXTRACTS

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Endang Lukitaningsih ◽  
Syamsu Nur ◽  
Fadilah Qonithah ◽  
Andi Zulbayu ◽  
Rina Kuswahyuning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Grapefruit Peel ◽  
Ferric Reducing Antioxidant Power ◽  
Tyrosinase Enzyme ◽  
Collagenase Inhibition

The research aims to analyse the antioxidant, anti-tyrosinase and anti-wrinkle activities from grapefruit (Citrus Maxima L) and strawberry extracts. Samples were extracted by maceration using 96% ethanol and ethyl acetate, subsequently. The Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching (BCB) were used to measure the antioxidant activity. The effect of anti-wrinkle was determined by testing the inhibition of elastase and collagenase enzyme, while anti-tyrosinase activity was analysed using mushroom tyrosinase enzyme. The results showed that strawberry extracts in ethanolic (SE) and ethyl acetate (SEA) have antioxidant activity in FRAP (EC50 = 404.39 ± 3.27 µg / mL and 1978.65 ± 37.25 µg/mL) and BCB (IC50 = 292.30 ± 4.69 µg/mL and 671.11 ± 6.74 µg/mL). Whereas the grapefruit peel extracts both in ethanolic (GPE) and ethyl acetate (GPEA) have antioxidant activity in FRAP (EC50 219.47 ± 71.96 µg / ml and 309.44 ± 95.76 µg/ml) and BCB (EC50 245.19 ± 162.47 µg/ml and 567.54 ± 95.31 µg/ml). As positive standards for FRAP antioxidant analysis were quercetin and vitamin C which has IC50 respectively 18.97 ± 4.50 µg/mL and 24.47 ± 1.44 µg/mL. While in BCB analysis, Butylated Hydroxy Toluene (BHT) used as positive standard (IC50 38.68 ± 5.70 µg/mL). The samples of SE, SEA, GPE and GPEA showed tyrosinase inhibitory activity which the IC50 values were respectively 492.68 ± 1.43; 2658 ± 48.08; 3312.5 ± 222.74; 2985.17 ± 122.80 µg/ml. Kojic acid (IC50 111.52 ± 0.42 µg/ml) is used as positive standard in this study. In addition, SE, SEA, GPE and GPEA were able to inhibit elastase and collagenase enzymes, although their activities were still lower than the positive standard used in this study. Elastastinal in concentration 50 µg/mL giving elastase inhibition about 71.71 ± 0.81 µg/mL, while vitamin C in the same concentration showed collagenase inhibition about 66.79 ± 1.23 µg/mL. It can be concluded that the extract of strawberry and grapefruit peel has antioxidant, anti-tyrosinase and anti-wrinkle activity through inhibition of elastase and collagenase enzymes; thus, they can be used as antiaging cosmetic ingredients.

scholarly journals Aktivitas Antioksidan dan Fitokimia dari Ekstrak Etil Asetat Pucuk Idat (Cratoxylum glaucum)

2018 ◽  
Vol 5 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Natural Antioxidant ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidants ◽  
Synthetic Antioxidant ◽  
Phytochemical Content

Neutralizing free radicals can use both synthetic and natural antioxidant compounds. Although synthetic antioxidant compounds are more active, but lately the use of synthetic antioxidants is being reduced because they are reported to have carcinogenic side effects. Natural antioxidant compounds found in plants are phenolic groups such as flavonoids, tannins, xanthones, and anthraquinones. This compound is widely found in the genus Cratoxylum where one of the species is pucuk idat (Cratoxylum glaucum). Pucuk idat are often used by people of Bangka as a flavoring dish and are believed to be traditional medicine to facilitate breastfeeding, tighten skin, treat fever, cough, and diarrhea. The increasing of antioxidants needs and lots efficacy of pucuk idat, hence this study aims to determine the antioxidant activity of ethyl acetate extract Cratoxylum glaucum. The antioxidant test in this study used the DPPH (Diphenylpicrylhydrazyl) method and vitamin C as a positive control. The results of this study indicate that the extract of ethyl acetate Cratoxylum glaucum has strong antioxidant activity with value IC50 32,213 μg / mL. Phytochemical content itself includes hydroquinone phenols (tannins), flavonoids, and steroids. This shows that ethyl acetate extract Cratoxylum glaucum can be used as natural antioxidant.

scholarly journals In-Vitro Study of Antioxidant Activities from Ethanol Extracts of Akar Kuning (Arcangelisia flava)

2019 ◽  
Vol 4 (2) ◽  
pp. 66-71
Author(s):  
Suratno Suratno ◽  
Muhammad Ikhwan Rizki ◽  
Mohammad Rizki Fadhil Pratama
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Ic50 Values ◽  
Stem Extract ◽  
Ethanol Extracts

This study aims to determine the antioxidant activity of akar kuning (Arcangelisia flava) stem extract from Central Kalimantan using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. This research includes extraction of simplicia and test of antioxidant activity by DPPH method. Based on the analysis of antioxidant activity on ethanol extract from yellow root plant stem IC50 values of 136.81 ppm were obtained. This IC50 value indicates the antioxidant strength of the yellow root plant stems including in the moderate category. Further research on antioxidant activity in the fraction of akar kuning stems needs to be done to determine the composition of the antioxidant compounds in each solvent.

scholarly journals Liberica Coffee (Coffea liberica L.) from Three Different Regions: In Vitro Antioxidant Activities

2021 ◽  
Vol 11 (5) ◽  
pp. 13031-13041
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Unstable Molecules ◽  
Coffea Liberica

Free radicals are unstable molecules with unpaired electrons in their outer orbitals. An antioxidant is a compound that can be scavenged free radicals. Coffee is one of the natural antioxidants. This research aimed to study the antioxidant activity of medium roasted beans of liberica coffee (Coffea liberica) from three different regions by DPPH and CUPRAC methods. To determine total phenolic content (TPC) and total flavonoid content (TFC), analyze the correlation between TPC and TFC with AAI DPPH and CUPRAC and the correlation between two methods in sample extracts. The sample was extracted by reflux using n-hexane, ethyl acetate, and ethanol. AAI DPPH in the range of 0.397- 18.536, while CUPRAC 0.532-4.674. The highest TPC in ethanol extract of liberica coffee from Aceh (22.585 ± 1.610 g GAE/100 g) and the highest TFC in ethyl acetate extract of liberica coffee from Aceh (4.927 ± 0.355 g QE/100 g). TPC of all samples had a positive and significant correlation with AAI DPPH and CUPRAC. AAI DPPH and CUPRAC value gave a significant and positive correlation. TPC of liberica coffee from three different regions contributed to antioxidant activity by DPPH dan CUPRAC methods. The two methods showed linear results in antioxidant activities of liberica coffee from three regions.

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