scholarly journals EFFECT OF DIFFERENT LEVEL OF PROTEIN ON GROWTH PERFORMANCE, SURVIVAL RATE, DIGESTIVE ENZYME, AND BODY PROTEIN COMPOSITION OF JUVENILE BESENG-BESENG FISH (Marosatherina ladigesi)

2020 ◽  
Vol 9 (1) ◽  
pp. 1047
Author(s):  
Jayadi Jayadi ◽  
Amrah Husma ◽  
Nursyahran Nursyahran
Keyword(s):  
Enzyme Activity ◽  
Survival Rate ◽  
Growth Performance ◽  
Protein Content ◽  
Digestive Enzyme ◽  
Protein Composition ◽  
Body Protein ◽  
Protein Levels ◽  
Protease Enzyme ◽  
Randomized Design

Marosatherina ladigesi’s original habitat is in the freshwater of South Sulawesi as endemic fish in the wallacea zone Indonesia, with a local name called beseng-beseng fish. M.ladigesi was already in the category of at threat of extinction.The purpose of this study was to investigate the effect of different protein levels on survival rate, growth performance, body protein composition and enzyme activity of protease, lipase and amylase of beseng-beseng fish. The research method was using Completely Randomized Design with three treatments and three replications. Treatment of the study: protein content of feed A: 40%, B: 50% and C: 60%. Fish maintenance takes 90 days. The results showed that protein content had a significant effect (P<0,05) on absolute body growth, specific growt rate, survival rate, body protein composition and protease enzyme activity of juvenile beseng-beseng fish. The best growth performance is obtained at 50% and 60% protein content, while the survival and body protein composition and the best protease enzyme activity at 60% protein content.

scholarly journals KEBUTUHAN PROTEIN PAKAN UNTUK PERFORMA OPTIMAL BENIH IKAN PATIN PASUPATI (PANGASIID)

2018 ◽  
Vol 13 (1) ◽  
pp. 47
Author(s):  
Evi Tahapari ◽  
Jadmiko Darmawan
Keyword(s):  
Survival Rate ◽  
Growth Performance ◽  
Protein Content ◽  
Maximum Growth ◽  
Feed Conversion ◽  
Protein Retention ◽  
Randomized Design ◽  
Significant Difference ◽  
Test Fish ◽  
Feed Protein

Penelitian ini bertujuan untuk mendapatkan kandungan protein optimum pakan untuk menunjang pertumbuhan maksimal dalam pemeliharaan benih ikan patin pasupati. Penelitian dirancang menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Sebagai perlakuan adalah pakan dengan kandungan protein berbeda, yaitu: A. 30%, B. 35%, C. 40%, dan D. 45%. Wadah pemeliharaan ikan uji adalah bak fiber berukuran 57 cm x 36 cm x 29 cm. Sebanyak 30 ekor ikan uji berukuran bobot awal 0,94 ± 0,13 g dengan panjang awal 3,82 ± 0,21 cm; ditebar ke dalam setiap bak. Percobaan berlangsung selama empat puluh hari. Ikan diberi pakan lima kali/hari sebanyak 10% dari biomassa ikan. Parameter yang diamati adalah laju pertumbuhan bobot, pertumbuhan panjang, retensi protein, rasio konversi pakan, dan sintasan. Data dianalisis dengan analisis ragam pada selang kepercayaan 95% dan dilanjutkan dengan Uji Beda Nyata Terkecil (Uji BNT). Hasil penelitian menunjukkan bahwa pakan dengan kandungan protein 40% (perlakuan C) memberikan performa pertumbuhan bobot, pertambahan panjang, retensi protein, dan konversi pakan terbaik (P<0,05) dibandingkan dengan perlakuan lainnya. Sintasan ikan pada perlakuan A, B, dan C berturut-turut sebesar 81,11%; 80,00%; dan 80,00% dan tidak berbeda nyata (P>0,05). Ikan pada perlakuan D memiliki sintasan terendah yaitu 72,22%.This study was aimed to determine the optimum feed protein content to support maximum growth performance of catfish juveniles. The study was designed using a completely randomized design (CRD) with four treatments and three replications. The treatments consisted of different protein contents of artificial feed, namely: A. 30%, B. 35%, C. 40%, and D. 45%. The test fish were reared in fiber tank containers sized 57 cm x 36 cm x 29 cm. A total of 30 fish with an initial weight of 0.94 ± 0.13 g and length of 3.82 ± 0.21 cm were stocked into each tank. The experiment lasted for forty days. Fish were fed with the experimental feeds five times/day as much as 10% of the fish biomass. The parameters observed were weight growth rate, growth length, protein retention, feed conversion ratio, and survival rate. The data were analyzed by analysis of variance at 95% confidence interval and continued with the least significant difference (LSD) test. The results showed that the feed with a crude protein content of 40% (treatment C) gave the best growth performance, protein retention and feed conversion and significantly different compared with the other treatments (P <0.05). The survival rate of fish in treatment A, B, and C were 81.11%, 80.00%, and 80.00%, respectively which was not significantly different (P>0.05). Fish in the treatment D had the lowest survival rate (72.22%).

scholarly journals Effects of Stocking Density and FeedingfFrequency on the Growth Performance, Digestive Enzyme Activity, and Tissues Histology of Hybrid Grouper (Epinephelus Fuscoguttatus♀×E. Lanceolatus♂)

2020 ◽  
Author(s):  
Yan Chen ◽  
Hai Huang ◽  
Jun Ma ◽  
Wenkan Liu ◽  
Honggan Zhong ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Weight Gain ◽  
Growth Performance ◽  
Protein Content ◽  
Specific Growth ◽  
Digestive Enzyme ◽  
Stocking Density ◽  
Feed Consumption ◽  
Digestive Enzyme Activity ◽  
Feeding Frequency

Abstract A 6-week 5×2 factorial study was conducted to examine the effects of stocking density and feeding frequency on growth performance, body composition, digestive enzyme activity, and tissue histology of juvenile hybrid groupers raised in an indoor circulating water system. Triplicate groups of fish were reared in tanks following a factorial design consisting of ten treatments including 5 stocking densities (SD) [1.1 kg m−3(0.55 kg fish per tank, SD1.1), 2.2 kg m-3(1.1 kg fish per tank, SD2.2), 3.3 kg m−3(1.65 kg fish per tank, SD3.3), 4.4 kg m−3(2.2 kg fish per tank, SD4.4) and 5.5 kg m−3(2.75 kg fish per tank, SD 5.5)] and 2 feeding frequencies (FF) [3 meals a day (FF3) and 2 meals a day (FF2)]. The resulting 10 treatments were G1(SD1.1,FF3), G2(SD2.2,FF3), G3(SD3.3,FF3), G4(SD4.4,FF3), G5(SD5.5,FF3), G6(SD1.1,FF2), G7(SD2.2, FF2), G8(SD3.3, FF2), G9(SD4.4, FF2) andG10 (SD5.5, FF2). Feed consumption and temperature were recorded throughout the experiment. After 6 weeks, the results indicated that the weight gain rate (WGR) and specific growth rate (SGR) of fish in the G7 group were significantly higher than those of other groups (P< 0.05), followed by G1, with G3 being the lowest. Weight gain and specific growth rates were generally higher in fish fed twice a day than those fed three times a day. The variations in protein content between groups were consistent with the muscle protein content trends. Feeding frequency and stocking density had significant effects on serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TGs), and cholesterol (CHO) (P < 0.05). Regarding immune function, grouper albumin (ALB), serum lysozyme (LZY), superoxide dismutase (SOD), and alkaline phosphatase (AKP) were significantly affected by stocking density and feeding frequency (P< 0.05). Pepsin and lipase activities in the stomach, intestine, and liver were also affected. The histological structure of the stomach, liver, and intestine in G1, G2, G7, and G8 fish was relatively normal, whereas those of the remaining groups exhibited varying degrees of damage. Overall, the optimum stocking densities were 1.343 kg/m3 (approximately 10 fish) and 2.004 kg/m3 (approximately 20-30 fish) when the fish were fed 3 and 2 times per day, respectively.

scholarly journals Enrichment Daphnia sp. with glutamin to improve the performance of the growth and survival rate of gurami Osphronemus goramy Lacepede, 1801 larvae

2019 ◽  
Vol 19 (3) ◽  
pp. 349
Author(s):  
Rizkan Fahmi ◽  
Mia Setiawati ◽  
Mas Tri Djoko Sunarno ◽  
Dedi Jusadi
Keyword(s):  
Antioxidant Activity ◽  
Survival Rate ◽  
Growth Performance ◽  
Physiological Response ◽  
Optimal Dose ◽  
Growth And Survival ◽  
Protease Enzyme ◽  
Randomized Design

This study aimed to evaluate the effectiveness of giving Daphnia sp. as a natural feed enriched with the doses of glutamine to increase growth performance and survival rate of larvae. This study used a completed randomized design which consisted of four treatments, namely 0, 25, 50, dan 75 mgL-1 and three replications. The test feed was given to larvae stocked with 60 fish/aquarium (40 cm x 30 cm x 30 cm) for 21 days. The results showed that significant on growth performance and survival rate were revealed on larvae fed with Daphnia sp. which enriched by glutamine dose of 25-75 mgL-1.The physiological response of protease enzyme and antioxidant activity showed that better performance was found in the treatment with addition of glutamine rather than control. In short, enrichment of Daphnia sp. using glutamine can improve the growth performance and survival rate of goramy larvae. The optimal dose of glutamine for improving the survival of gorami larvae is 25 mg L-1.

Digestive enzyme and gut surfactant activity of detritivorous gizzard shad (Dorosoma cepedianum)

2000 ◽  
Vol 57 (6) ◽  
pp. 1113-1119 ◽  
Author(s):  
James C Smoot ◽  
Robert H Findlay
Keyword(s):  
Enzyme Activity ◽  
Protease Activity ◽  
Feeding Strategy ◽  
Digestive Enzyme ◽  
Artificial Substrates ◽  
Gizzard Shad ◽  
Dorosoma Cepedianum ◽  
Digestive Physiology ◽  
Protease Enzyme ◽  
Surfactant Activity

Measuring digestive enzyme and surfactant activities tested specialization of gizzard shad (Dorosoma cepedianum) digestive physiology to a detritivorous feeding strategy. Digestive enzyme activity was measured in adult and larval gizzard shad using fluorescently labeled artificial substrates. Surfactant activity in gizzard shad was measured by comparing gut juice drop diameters over a range of dilutions. Enzyme activity in the ceca region of adult gizzard shad was high for esterase, beta-glucosidase, lipase, and protease. Enzyme activity was lower in posterior intestine sections than in anterior intestine sections, although protease activity remained high for the greatest distance in the intestine. Micelles were detected in adult gizzard shad gut juice, and surfactant activity was greatest in the ceca region. Larval gizzard shad protease activity was similar to that of adult fish, and surfactants were below their critical micelle concentration. Gizzard shad coupled digestive physiology with gut anatomy to obtain nutrients from detritus, and these adaptations may explain elevated growth rates observed in these fish when they are planktivorous.

scholarly journals TINGKAT KELANGSUNGAN HIDUP DAN RASIO KONVERSI PAKAN PADA BUDIDAYA IKAN GURAMI (Osphronemus goramy LAC.) DENGAN SISTEM BIOFLOK DAN PEMBERIAN PAKAN KADAR PROTEIN YANG BERBEDA

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Retno Wijayanti ◽  
Muarif Muarif ◽  
Dudi Lesmana
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Protein Level ◽  
Conversion Ratio ◽  
Artificial Feeding ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Protein Levels ◽  
Randomized Design ◽  
Completely Randomized Design

This research was carried out on March to August, 2018, and the experiment on April to June, 2018 at the Fishery Laboratory, Djuanda University Bogor. The objective of research is to know and analyze the survival rate and feed conversion ratio of Giant Gouramy that cultured on biofloc media and different protein levels. The experimental design that used is completely randomized design   with 2 treatments   and 6 replications.  The treatments are  treatment A (feeding of protein level  of 17% + mollase addition  with C,/N  ratio 12) and treatment B (feeding of protein  level  of  30%  without  mollase)  Fish that used is Giant Gouramy fry with length 5-7 cm that maintained cylinder container of diameter 1 m and height 1 m. The results of research that the artificial feeding of protein level of 17% + mollase addition with C/N ratio 12 gives the survival rate which is better, but the feed conversion ratio is bad. The average of the highest survival rate of Giant Gouramy is 89% (treatment A) and the low feed conversion ratio is 1.6 (treatment B). Water  quality during the research are temperature  of  23,6-30,0 °C, dissolved oxygen of 6.4- 8.8 mg/l and  pH of 6,3-8,8.Key words: Giant Gouramy, biofloc, feed conversion ratio, protein level.

Digestive physiological characteristics of black soldier fly larvae reared on five organic wastes

2021 ◽  
pp. 1-18
Author(s):  
Q.X. Sun ◽  
X.C. Li ◽  
X.H. Tan ◽  
Y.W. Dong ◽  
C.H. You ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Growth Performance ◽  
Food Source ◽  
Digestive Enzyme ◽  
Organic Wastes ◽  
Food Sources ◽  
Black Soldier Fly ◽  
Ph Values ◽  
Black Soldier Fly Larvae ◽  
Luminal Ph

Black soldier fly larvae (BSFL) are able to utilise a broad range of organic wastes to fulfil their growth needs. To acquire this basic knowledge of its digestive adaptation to various food, five organic wastes (soybean meal [SBM], wheat bran [WB], beer yeast [BY], kitchen waste [KW] and chicken manure [CM]) were fed to 3-day-old BSFL for 16 days. The growth performance, luminal pH of the gut, midgut histology, digestive enzyme activity and intestinal bacterial microbiota of the larvae were assessed. Growth performance was greatest in the KW group followed by the SBM group and was worst in the CM group. Nutrient compositions of larvae were not significantly affected by those of the food sources, with the exception of crude ash. The ultrastructural observations of midgut showed the number of mitochondria in CM and BY groups was less than other three groups. Twenty-four hours after feeding, weakly acidic, acidic, strongly acidic, and alkaline luminal pH values were detected in the anterior, middle and posterior segments of the gut in all groups, but the luminal pH values of the hindgut varied with food source. Significant lipase and esterase activity, cellulase activity, and amylase activity were detected in the intestines of larvae reared on KW, WB and BY, respectively, revealing that digestive enzyme activity was closely associated with the nutrient composition of the food source. Bacterial composition and diversity differed significantly between groups and were characterised by specific indicator bacteria, which may play important roles in food digestibility. The results suggested that different food sources induced adaptive physical, chemical and biological changes in the digestive tracts of BSFL and may indicate that BSFL developed specific strategies for nutrient utilisation and accumulation. The knowledge acquired here will be beneficial for developing rearing protocols to optimise bioconversion in this insect for their various applications.

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