scholarly journals Listeriosis Outbreak in Sheep Raised in Feedlots in the Southern Region of Rio Grande do Sul State, Brazil

2022 ◽  
Vol 50 ◽  
Author(s):  
Luiza Soares Ribeiro ◽  
Haide Valeska Scheid ◽  
Lucas Dos Santos Marques ◽  
Fabiano Da Rosa Venancio ◽  
Elisa Rocha Da Silva ◽  
...  

Background: A listeriosis outbreak in a sheep fattening feedlot in the Southern Region of Rio Grande do Sul State, Brazil is described. This disease is caused by Listeria monocytogenes and represents a risk to public health since it affects not only ruminants but also humans. This agent is widely spread in the environment, such as in the soil and water. It is also found in decaying vegetable matter and the feces and fluids of domestic animals. The aim of this study was to describe a listeriosis outbreak in sheep raised in feedlots, its epidemiology, and to establish the importance of this disease in this type of sheep management system, evaluate the possible sources of infection, and suggest ways to control it.Cases: Sheep were kept in a 2-sector shed, one with east solar orientation and the other with west solar orientation, the latter with free access to domestic birds. Sheep were fed silage and concentrate. Seven sheep were affected, 5 died and 2 recovered. Clinically, the sheep displayed loss of balance, excessive drooling, and tremors; one exhibited circling, head deviation, apathy, nystagmus, lateral recumbency, paddling, and labored breathing. At necropsy, macroscopic lesions were not found, and histologically several micro-abscesses and perivascular cuffs with lymphocytes, macrophages, and neutrophils were present in the brain stem. Listeria monocytogenes suspected colonies were observed in the microbiological culture, and the bacteria was identified by biochemical analysis. The immunohistochemistry test in brain stem sections was positive for the antibody BD DifcoTM Listeria O Antiserum Poly Serotypes 1 and 4.Discussion: A listeriosis outbreak in a feedlot sheep was confirmed through epidemiological findings, histological lesions, bacterial culture, and immunohistochemistry analysis. This infection is frequent in sheep fed silage of poor quality or other food with improper storage and lack of hygiene. In the present outbreak, the bacteria were isolated from silage. However, it is likely that domestic birds, which were raised in the same place and had free access to the west sector of the feedlot, were the initial source of infection, because the sheep from the opposite sector (east) did not get ill. The disease caused by environmental contamination or through contact with fluids and feces of ducks, chickens, cattle, and pigs has already been described in outbreaks that occurred in the central region of Rio Grande do Sul. Sheep aggregation in feedlot systems is also a favorable factor for the development of the bacteria and the occurrence of outbreaks because the accumulation of feces and urine cause humidity on the stalls. This allows outbreaks to occur in other seasons of the year, such as in the outbreak reported herein. The exchange of silage that served as food for sheep was another control measure, and new cases were not reported 8 months after these procedures were taken. In the outbreak studied, 2 sheep that exhibited clinical signs were treated with oxytetracycline and recovered. Some authors report that treatment for listeriosis is inefficient because neurological lesions are irreversible. Nonetheless, other studies have reported the recovery of some animals when they were treated with oxytetracycline or a combination of oxytetracycline and dexamethasone or ampicillin and gentamicin like in the outbreak described in this paper.Keywords: Listeria monocytogenes, central nervous system, silage, feedlot, ovine.

2020 ◽  
Vol 48 ◽  
Author(s):  
Antônio Amaral Barbosa ◽  
Ederson Dos Santos ◽  
Rosana Klaus ◽  
Viviane Rohrig Rabassa ◽  
Mauro Pereira Soares ◽  
...  

Background: Poisoning by Xanthium spp. plants are important causes of deaths of cattle in the state of Rio Grande do Sul. This plant has as toxic principle a triterpenoide glycoside that acts inhibiting the transport of adenosine diphosphate and adenosine triphosphate across the mitochondrial membrane, responsible for causing acute liver failure. Clinical signs occur within hours of ingesting the plant. However, the knowledge of the metabolic disorders that these animals suffer are poorly described, so the objective of the reports is to describe an outbreak of spontaneous intoxication by Xanthium cavanillesii and demonstrate the clinical, metabolic and anatomopathological changes of a poisoned bovine.Case: An 8-month-old male bovine, Aberdeen Angus breed, was sent to the Veterinary Hospital of Clinics of Federal University of Pelotas (HCV-UFPel). The animal came from a property located in the southern region of Rio Grande do Sul, belonged to a herd of 506 animals, of which 258 were nulliparous cows and 248 calves, in total 92 died, being 20 cows and 62 calves in a period of 3 days. The clinical course ranged from 3 to 72 h and was characterized by neurological signs (pedaling movements, opisthotonus and motor incoordination), sternal decubitus, muscle tremors, apathy, anorexia, hypersalivation, dehydration, tenesmus and death. In the field where the animals were, there was the present of Xanthium cavanillesii in the dicotyledonouns sprouting phase, with signs of ingestion. During the clinical examination of the animal sent to the hospital, sternal decubitus, muscle tremor, motor incoordination, apathy, anorexia, hypersalivation and opisthotone were observed, heart rate of 60 beats per minute, respiratory rate of 15 movements per minute, 2 ruminal movements incomplete at every 2 minutes, moderate dehydration and temperature of 37.9º C. For laboratory exams, blood samples were collected in vacuum tubes with 10% EDTA, to perform blood count with hemoparasite research, and without anticoagulant, to perform serum biochemistry tests, liver and kidney function. Highlighting the increase in liver enzymes (aspartate aminotransferase, gamma glutamyltranferase and alkaline phosphatase), hypoglycemia, elevation of total bilirubin and direct bilirubin, hypoalbuminemia, increase in triglycerides with leukocytosis by neutrophilia with regenerative left shift. Due to advanced clinical condition of the animal, was realized euthanasia for post-mortem examination. At necropsy, the main diagnostic macroscopic lesions were an accentuation of the lobular pattern of the organ parenchyma with the appearance of “nutmeg” and centrilobular coagulative necrosis accompanied by congestion and hemorrhages, characterizing a picture of acute liver failure. In addition, X. cavanillesii fruits were detected in the rumen content.Discussion: The diagnosis was based on epidemiological, clinical-anatomopathological, laboratory data and experimental reproduction of the disease in cattle and sheep. Regarding the epidemiological findings, the presence of the plant with signs of ingestion stands out in addition to the absence of other plants capable of producing similar cases on the farm. The metabolic changes described, if analyzed in isolation, do not confirm the diagnosis, but combined with other parameters, it contributes to the assessment and prognosis of the patient, becoming of great importance in elucidating and conducting the clinical condition.


Food Control ◽  
2012 ◽  
Vol 28 (1) ◽  
pp. 171-177 ◽  
Author(s):  
Karla Sequeira Mendonça ◽  
Geovana Brenner Michael ◽  
Ana Eucares von Laer ◽  
Dulcinea Blum Menezes ◽  
Marisa Ribeiro Itapema Cardoso ◽  
...  

2017 ◽  
Vol 26 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Plínio Aguiar de Oliveira ◽  
Beatriz Riet-Correa ◽  
Pablo Estima-Silva ◽  
Ana Carolina Barreto Coelho ◽  
Bianca Lemos dos Santos ◽  
...  

Abstract Gastrointestinal parasites represent an important cause of reduced productivity of sheep worldwide. As anthelmintic are still the main control tool for these parasites, this work evaluated the efficacy of commercially available active principles in 22 sheep flocks in the southern region of Rio Grande do Sul, Brazil. In each farm 10 sheep were randomly distributed in seven groups with the following treatments: abamectin; albendazole; closantel; levamisole; monepantel; trichlorphon and no anthelmintic (control). All flocks showed resistance to at least three anthelmintics and in 20 farms only two products demonstrated efficacy for parasitic control. In two farms, there was no susceptibility to the six active principles tested. The results of this study provide evidence that the common commercially available anthelmintic are not assuring effective chemical control of gastrointestinal parasitic infections in ovine flocks in the southern region of Rio Grande do Sul. Monepantel, the newest introduced drug in the Brazilian market was not effective in 18% of the flocks tested, confirming that the parasitic resistance can be established quickly after the introduction of new molecules mainly when alternative program of parasite control is not performed.


2017 ◽  
Vol 37 (12) ◽  
pp. 1443-1452
Author(s):  
Ronaldo M. Bianchi ◽  
Welden Panziera ◽  
Tatiane C. Faccin ◽  
Gisane L. de Almeida ◽  
Juliana F. Cargnelutti ◽  
...  

ABSTRACT: This article describes the clinical, pathological and epidemiological aspects of 17 outbreaks of bluetongue (BT) disease in sheep occurring between December 2014 and July 2015 in the central region of Rio Grande do Sul state (RS), southern Brazil. Affected farms were visited for clinical examination, necropsy, sample collection and epidemiological investigation. The outbreaks were seasonal and occurred during the summer and autumn. A total of 180 sheep (20.4%) out of 884 in 17 small herds were affected. All ages of Texel and mixed breed sheep were affected. However, lambs (younger than one year) had higher morbidity than adult sheep. The most frequent clinical signs were anorexia, lethargy, loss of body condition, facial swelling mainly involving the lips, and greenish seromucous or mucous nasal discharge. Pulmonary lesions characterized by edema were the most prevalent findings; however, erosive and ulcerative lesions in the upper gastrointestinal tract, as well as cardiac, skeletal muscle and esophageal striated muscle necrosis, and hemorrhage in the pulmonary artery were also frequent. The bluetongue virus (BTV) genome was detected by RT-PCR in blood and tissue samples (spleen and lungs) of 21 animals from 17 outbreaks. The virus involved in the outbreak 3 was subsequently isolated and shown to belong to serotype 17, for the first time reported in Brazil. In summary, our data support the BTV genotype 17 as the etiological agent of the outbreaks and indicate that the central region of RS is an area at risk for BT in sheep, a disease previously not recognized in the region.


2013 ◽  
Vol 43 (5) ◽  
pp. 878-883
Author(s):  
Jader Silva Lopes ◽  
Paulo Roberto Nogara Rorato ◽  
Tomás Weber ◽  
Ronyere Olegário de Araújo ◽  
Dionéia Magda Everling ◽  
...  

The objective of this study was to evaluate the genetic divergence among Nellore breed animals raised in 45 farms in the Southern Region of Brazil. The characteristic studied was weaning weight adjusted to 205 days of life (P205), from 10,874 animals sired by 425 bulls and 7,629 cows, collected between 1976 and 2001, and distributed in the states of Rio Grande do Sul (1,499), Santa Catarina (2,332) and Paraná (7,043). The animals were distributed by cluster analysis in eight genetic divergent groups, enabling this technique to be applied to organize the matings in order to obtain heterotic effect. The herd/farm groups were formed through the hierarchical Ward method, using the direct (VGD) and maternal (VGM) breeding values predicted by the REML method. The VGD of the animal accounted for 90% of the differences among herds, and the remaining 10% was attributed to differences in the VGM. On average, the P205 for the animals from inter-group mating was 1.4kg higher than those from intra-group mating, representing 2.4% of heterosis.


2019 ◽  
Vol 47 ◽  
Author(s):  
Stéfano Leite Dau ◽  
Tanise Policarpo Machado ◽  
Ezequiel Davi Dos Santos ◽  
Diorges Henrique Setim ◽  
Eduardo Rebelato Sakis ◽  
...  

Background: In various regions of Brazil, horses and cattle are considered the most susceptible animals to plant poisoning. The plants of the genus Senecio are the most important in Rio Grande do Sul because they have the active principle known as the pyrrolizidine alkaloid. Its diagnosis is made through epidemiology, clinical signs and histopathological analysis, either of the tissues obtained by biopsy or necropsy. The objective of this study was to report and characterize the epidemiological, clinical and anatomopathological findings of three cases of natural poisoning by Senecio brasiliensis in horses assisted at the Hospital Veterinário (HV) of the Universidade de Passo Fundo (UPF).Cases: Three traction horses, two males and one female, were admitted at the HV-UPF for clinical care. The animals were presenting anorexia, weight loss, dysmetria, proprioceptive deficit and signs suggestive of colic. The epidemiological study revealed that the sites where these animals were located were infested by Senecio brasiliensis. The support therapy used for equine colic in all three cases was unsuccessful. One of the animals died and the other two were euthanized, all three of them being reffered for necropsy. The post-mortem findings were mainly found in the liver, which showed accentuation of the lobular pattern and the appearance of nutmeg. During necropsy, fragments of organs from thoracic and abdominal cavities and central nervous system were collected and fixed in 10% buffered formalin. Subsequently, the samples were processed chemically, submitted to cuts of five micrometers of thickness and stained with hematoxylin and eosin for microscopic analysis. Microscopically, the liver of all three horses presented megalocytosis, fibrosis and bile ducts hyperplasia. In the central nervous system, spongiosis and the presence of Alzheimer type II astrocytes were observed. Thus, through the association of information, the diagnosis of poisoning by Senecio brasiliensis was achieved.Discussion: The diagnosis of natural poisoning by Senecio brasiliensis was obtained through the epidemiological survey that showed S. brasiliensis in pastures where all three horses were allocated.  Cases of intoxication by S. brasieliensis in cattle are more frequent than in equines, although both species are considered the most susceptible. In horses, the main clinical manifestations observed include neurological disorders, apathy, anorexia, dysphagia, weight loss, subcutaneous edema and icterus. The clinical signs presented by the equines suggested initial signs of colic syndrome, although anorexia, weight loss, dysmetria and proprioceptive deficit are commonly observed in pyrrolizidine alkaloid poisoning in the liver, both in cattle and horses. The pyrrolizidine alkaloids present in the genus Senecio that become toxic when biotransformed in the liver into a pyrrholic form highly reactive that inhibits cell mitosis and leads to the onset of megalocytosis, cell death and liver fibrosis. The necropsy findings and histopathology were characteristic of poisoning in equines, since the predominant macroscopic lesions in the liver were hepatomegaly and accentuation of lobular pattern, whereas microscopically, there was a predominance of hepatic fibrosis, megalocytosis, spongiosis and the incidence of Alzheimer's type II astrocytes in the brain. These lesions are observed both in natural and in experimental cases of poisoning in horses. Thus, through the ante-mortem and complete post-mortem evaluation of the three equines, it was possible to establish the occurrence of natural poisoning by Senecio brasiliensis in Northern Rio Grande do Sul, as well as to characterize the epidemiological, clinical and anatomopathological findings of poisoning in this species.


2018 ◽  
Vol 4 (6) ◽  
pp. 107
Author(s):  
Talita Gonçalves MEDEIROS ◽  
Marcio CAETANO

O presente estudo possuiu como objetivo interrogar e compreender as representações sobre a(s) lesbianidade(s) produzidas por estudantes de uma escola agrícola da região sul do estado do Rio Grande do Sul. A pesquisa, orientada pelos Estudos Culturais Lesbofeministas, produziu seus dados a partir de “rodas de conversas” complementadas por anotações no “diário de campo”. Partindo das análises dos dados, podemos apontar que as estudantes possuem visões e entendimentos conceituais a respeito da(s) lesbianidade(s) e que esses já possuem um posicionamento crítico frente à forma como a mulher é retratada na sociedade. Entretanto, a temática “lesbianidade(s)” – não diferente da forma como as demais mulheres são retratadas na escola-, é atravessada pela invisibilidade histórico-escolar e quando visível, ancora-se em representações mediadas somente pela violência.Lesbianidade(s). Escola. Diálogo. Lesbofeminismo.Obscure Things: representations of high school girls about lesbianity(s)AbstractThe present study aimed to interrogate and understand the representations about the lesbianity (ies) produced by students of an agricultural school in the southern region of Rio Grande do Sul state. The research, guided by the Lesbofeminist Cultural Studies, produced its data through "circles of conversation" supplemented by notes of the "field diary". From the analysis of the data, we can point out that the students have conceptual visions and understandings about the lesbian(s) and that they already have a critical position regarding the way in which the woman is portrayed in society. However, the theme of "lesbianity (ies)" - not unlike the way other women are represented in school - is crossed by the invisibility at school space and, when visible, is anchored in representations mediated only by violence.Lesbianity (s).School. Dialogue. Lesbofeminismo


2004 ◽  
Vol 9 (1) ◽  
Author(s):  
D.S. PRESTES ◽  
A.R. FILAPPI ◽  
M. CECIM

O presente estudo objetivou analisar os níveis plasmáticos de cálcio (Ca), fósforo inorgânico (Pi), cobre (Cu) e zinco (Zn) em vacas de corte primíparas e multíparas, mantidas em campo nativo e suplementadas com misturas minerais comerciais completas, em rebanhos na região Centro-Oeste do Rio Grande do Sul. Amostras sangüíneas foram colhidas nos períodos de Final de Gestação (FG), Pico da Lactação (PL) e Entoure (E). As médias plasmáticas dos elementos minerais analisados estiveram conforme as referências, com exceção do Pi, nas vacas primíparas, no período de E. Entre períodos, nas vacas primíparas, houve diferença significativa nos níveis de Ca, sendo maiores no PL do que no FG; quanto ao Cu, os níveis foram significativamente maiores no E em relação ao FG; no Zn os níveis foram significativamente menores no PL. Nas multíparas, os níveis de Zn foram significativamente menores no período de PL em relação FG. Entre categorias, os níveis de Ca foram significativamente maiores nas primíparas, no PL; quanto ao Cu, os níveis foram significativamente maiores nas primíparas, no período de PL e E. Nas condições em que o estudo foi conduzido, conclui-se que a suplementação mineral completa provém níveis plasmáticos marginais de cálcio, fósforo inorgânico e cobre em ambas as categorias e em todos os períodos produtivos. Mesmo com suplementação mineral irrestrita, o período produtivo exerce influência sobre os níveis plasmáticos de cálcio, cobre e zinco, nas vacas primíparas. Mineral status of supplemented beef herds in Mid-West Rio Grande do Sul State Abstract The objective of the present study was to analyze plasma levels of calcium (Ca), inorganic phosphorus (Pi), magnesium (Mg), copper (Cu), and zinc (Zn) in heifers and mature cows maintained in native pastures with free access to a complete mineral mixture. Blood samples were collected at the end of gestation (FG), at peak of lactation (PL) and during breeding season (E). The mean values for all minerals were around the lower limit of international reference ranges, with exception of Pi in first calf heifers during breeding. Among periods in heifers, Ca was higher during PL than in FG. Copper levels were higher in E than in FG while zinc values were lowest in PL than in the other two periods. Between categories, Ca levels were higher in PL heifers, and Cu was higher during PL and E than in FG. The present results suggest that supplementation with a complete mineral mix sustained only marginal plasma levels of Ca, Pi, and Cu in both groups in all periods studied. Even with free access to mineral supplement, the period of the productive cycle has an influence on plasma levels of Ca, Cu and Zn in first calf heifers.


2007 ◽  
Vol 37 (3) ◽  
pp. 862-867 ◽  
Author(s):  
Cristina Bergman Zaffari ◽  
Jozi Fagundes Mello ◽  
Marisa da Costa

O consumo de queijo artesanal, vendido em estabelecimentos de beira de estrada, é comum no Estado do Rio Grande do Sul. Geralmente estes produtos não são fabricados em acordo com as boas normas de fabricação e podem constituir perigo à saúde do consumidor. Objetivou-se, com o presente trabalho, verificar a qualidade bacteriológica de queijos artesanais comercializados em estradas litorâneas por meio da contagem de coliformes e pesquisa de Listeria spp. e Brucella spp. Foram analisados 80 queijos, sendo 62 do tipo Colonial, dez do tipo Provolone, seis do tipo Ricota e dois do tipo Caccio Cavallo. No momento da coleta, 71% das amostras não estavam sob refrigeração. Todas as amostras apresentaram contagens de coliformes totais e, destas, 62 foram testadas para a presença de coliformes fecais. Um total de 84% das amostras apresentou contagens de coliformes fecais acima de 2,73- 3,7 log.UFC g- 1 (de 500 a 5000UFC mL-1), previsto como limite máximo a ser encontrado em queijos. Dos 29 estabelecimentos, 27 tinham produtos fora destes limites. Das 80 amostras, 16% continham Listeria spp., sendo 3,7% identificadas como Listeria monocytogenes. As estações do ano influenciaram no isolamento de Listeria spp., sendo a primavera considerada a estação do ano com maior número de isolados. Brucella spp. não foram isoladas nas 80 amostras de queijos analisadas. A alta freqüência de coliformes fecais e a presença de L. monocytogenes revelam que o consumo destes queijos constitui perigo de infecção à população em geral e especialmente àquelas pessoas imunocomprometidas.


2010 ◽  
Vol 40 (3) ◽  
pp. 686-689 ◽  
Author(s):  
Jackeline Karsten Kirinus ◽  
Carina Krewer ◽  
Diego Zeni ◽  
Fernanda Monego ◽  
Marcia Cristina da Silva ◽  
...  

A listeriose é uma doença infecciosa que afeta uma grande variedade de espécies animais, causando septicemia, encefalite e aborto. As chinchilas são os animais mais susceptíveis à infecção sistêmica por Listeria monocytogenes. Este relato descreve um surto de listeriose sistêmica em uma criação de chinchilas da região central do Estado do Rio Grande do Sul, onde cerca de 16% das chinchilas morreram. Na necropsia, havia múltiplos focos brancos, pequenos e de distribuição aleatória nas superfícies capsular e de corte do fígado e aumento de volume do linfonodo hepático. Histologicamente, observaram-se hepatite necrossupurativa e linfadenite supurativa multifocais, com numerosos bacilos intralesionais. L. monocytogenes foi o agente etiológico do surto de listeriose sistêmica, sendo o diagnóstico confirmado por meio das lesões de necropsia e histopatológicas, das características fenotípicas e genotípicas da bactéria e da técnica de imuno-histoquímica.


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