Effect of led lighting on the growth of raspberry (Rubus idaeus l.) plants in vitro

In recent years, the light emitting diodes (LED) have become an alternative to the fluorescence lamp source of light for plant tissue culture, due to their low energy consumption, low heat emission, specific wavelength irradiation etc. The aim of this study was to investigate the effect of LEDs on the growth of in vitro cultivated raspberry (Rubus idaeus L. ‘Lloyd George’). The plantlets were cultivated in vitro under an illumination system based on Philips GreenPower LED research module. Four groups of LEDs emitting in white (W), red (R), blue (B), mixed (W:R:B:far-red=1:1:1:1) lights and fluorescent lamps (control) were used in our studies. Growth parameters, some physiological and biochemical characteristics of the plantlets were measured after three four weeks passages under corresponding light treatment. Our results indicated that different LEDs specifically influence the growth and development of in vitro cultivated raspberry plantlets and could be applied as an efficient lighting system for rapid in vitro micropropagation of Rubus idaeus L. The combination of blue, red, far red and white LEDs (1:1:1:1) stimulated the growth and biomass accumulation, as well as the intensity of net photosynthesis. For optimal results, it would be advisable to shorten the culture period to 3 weeks. This effective and affordable protocol would support the commercial micropropagation of raspberries and other soft fruits.

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Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

Food Processing Techniques and Technology ◽

10.21603/2074-9414-2021-3-520-528 ◽

2021 ◽

pp. 520-528

Author(s):

Sergey Makarov ◽

Sergey Rodin ◽

Irina Kuznetsova ◽

Anton Chudetsky ◽

Svetlana Tsaregradskaya

Keyword(s):

In Vitro Rooting ◽

Light Emitting ◽

Clonal Micropropagation ◽

Fluorescent Lamps ◽

Planting Material ◽

Medicinal Value ◽

American Cranberry ◽

Food Market ◽

Spectral Ranges

Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.

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Quality and Intensity of Light in the In Vitro Development of Microstumps of Eucalyptus urophylla in a Photoautotrophic System

Forest Science ◽

10.1093/forsci/fxaa027 ◽

2020 ◽

Vol 66 (6) ◽

pp. 754-760 ◽

Cited By ~ 1

Author(s):

Natane A Miranda ◽

Aloisio Xavier ◽

Wagner C Otoni ◽

Ricardo Gallo ◽

Kellen C Gatti ◽

...

Keyword(s):

Plant Growth ◽

Light Quality ◽

Stomatal Density ◽

Carotenoid Content ◽

Fluorescent Light ◽

Eucalyptus Urophylla ◽

Light Emitting ◽

Fluorescent Lamps ◽

Number Of Shoots

Abstract The quality and quantity of light are important factors in controlling in vitro plant growth in photoautotrophic systems. The aim of this study was to evaluate the influence of light quality (fluorescent, white, red, blue, red/blue, and distant red) on microstumps of a Eucalyptus urophylla clone in an in vitro photoautotrophic system, as well as the intensity of fluorescent light (60, 85, 100, and 140 μmol m–2 s–1) in the growth and production of microcutting. The number of shoots and microcutting, the size of the largest shoot, the stomatal density, chlorophyll, and carotenoid content were analyzed. Light quality altered plant growth, and fluorescent light intensity did not affect the microstumps’ production during the evaluation period. In white light-emitting diode (LED) light, there was higher production of carotenoids, with a lower initial production of microcuttings. A smaller number of shoots were obtained in blue LED. In general, the different qualities and light intensities tested allowed for the growth of the Eucalyptus urophylla clone grown in vitro, making it possible to obtain microcuttings under photoautotrophic cultivation. Study Implications In vitro propagation is a stressful process for plants and has limitations for commercial-scale Eucalyptus production. Fluorescent lamps, closed containers, and high sucrose concentrations are traditionally used. To reduce costs and improve production, the use of efficient light sources and photoautotrophic cultivation systems become alternatives. This study investigated the influence of light on the in vitro growth of a Eucalyptus clone in a photoautotrophic system. The quality was more important than the intensity of light. Foresters will be able to indicate the use of LEDs (light-emitting diodes) as a replacement for fluorescent lamps. This approach is useful in enhancing micropropagation techniques.

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Effects of Frequency and Duty Ratio on the Growth of Potato Plantlets In Vitro Using Light-emitting Diodes

HortScience ◽

10.21273/hortsci.39.2.375 ◽

2004 ◽

Vol 39 (2) ◽

pp. 375-379 ◽

Cited By ~ 38

Author(s):

Ruey-Chi Jao ◽

Wei Fang

Keyword(s):

Plant Growth ◽

Light Emitting Diodes ◽

Heat Removal ◽

Duty Ratio ◽

Intermittent Light ◽

Light Emitting ◽

Fluorescent Lamps ◽

Fluctuating Light ◽

Potato Plantlets

Effects of intermittent light on photomixotrophic growth of potato plantlets in vitro and the electrical savings that could be realized by adjusting the frequency and duty ratio of light-emitting diodes (LEDs) were investigated and compared to the use of conventional tubular fluorescent lamps (TFLs). TFLs provide continuous fluctuating light at 60 Hz and LEDs provide continuous nonfluctuating or intermittent/pulse light depend on the preset frequency and duty ratio. In total, eight treatments were investigated with varying light source, frequency, duty ratio and photoperiod. Results indicated that if growth rate is the only concern, LEDs at 720 Hz [1.4 milliseconds (ms)] and 50% duty ratio with 16-h light/8-h dark photoperiod stimulated plant growth the most. However, if energy consumption is the major concern, using LEDs at 180 Hz (5.5 ms) and 50% duty ratio with 16-h light/8-h dark photoperiod would be the best choice for illuminating potato plantlets without significantly sacrificing plant growth, especially when energy for heat removal is also considered.

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Optimizing Carbon Dioxide and Light Levels during in Vitro Culture of Theobroma cacao

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.119.4.865 ◽

1994 ◽

Vol 119 (4) ◽

pp. 865-871 ◽

Cited By ~ 11

Author(s):

Antonio Figueira ◽

Jules Janick

Keyword(s):

In Vitro Culture ◽

Theobroma Cacao ◽

Growth Parameters ◽

Net Photosynthesis ◽

Shoot Elongation ◽

Fresh Weight ◽

Ethylene Concentration ◽

Light Levels ◽

Number Of Leaves

In vitro culture of axillary cotyledonary shoots of Theobroma cacao L. (cacao) under increasing CO2 concentration from ambient to 24,000 ppm (culture tube levels) significantly increased total shoot elongation, number of leaves, leaf area per explant, and shoot dry and fresh weight. Although light was necessary for the CO2 response, the effect of various photon fluxes was not significant for the measured growth parameters. Net photosynthesis estimated on the basis of CO2 depletion in culture tubes increased 3.5 times from 463 to 2639 ppm CO2, and increased 1.5 times from 2639 to 14,849 ppm CO2, but declined from 14,849 to 24,015 ppm CO2. Ethylene concentration in culture vessels increased under enriched CO2 conditions. Depletion of nutrients (fructose, K, Ca, Mg, and P) from the medium was increased under enriched CO2 conditions.

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Ca[Mg3SiN4]Ce3+ phosphor: effect of particle concentration on lighting properties of the 7000K IPW-LEDs

International Journal of Power Electronics and Drive Systems (IJPEDS) ◽

10.11591/ijpeds.v11.i2.pp566-570 ◽

2020 ◽

Vol 11 (2) ◽

pp. 566

Author(s):

Q. S. Vu ◽

Tang Tin Dao ◽

Minh Tran

Keyword(s):

Energy Efficiency ◽

Particle Concentration ◽

Real Life ◽

Environment Friendly ◽

Light Emitting ◽

White Leds ◽

Co Doping ◽

Fluorescent Lamps ◽

Long Lifetime ◽

White Light Emitting Diodes

Nowadays, the white-light-emitting diodes (LEDs) have a vast application on the real-life based on its superior advantages such as energy efficiency, long lifetime, compactness, and environment-friendly and designable features in comparison with incandescent and fluorescent lamps. In this paper, we co-doping the Ca[Mg3SiN4]Ce3+ Phosphor on the phosphor compound of the 7000K In-cup Packaging White LEDs (IPW-LEDs) for improving the lighting properties. By varying the concentration of Ca[Mg3SiN4]Ce3+ Phosphor from 0% to 1.8%, the effect of the Ca[Mg3SiN4]Ce3+ Phosphor on the D-CCT, CRI, CQS, and LO of the 7000K IPW-LEDs are investigated. Using the Light Tool and software, the research results show that the concentration of the IPW-LEDs has a massive influence on the lighting properties of the 7000K IPW-LEDs. All the results are convinced by Light Tool simulation.

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Growth Quality and Development of Olive Plants Cultured In-Vitro under Different Illumination Regimes

Plants ◽

10.3390/plants10102214 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2214

Author(s):

Pablo Díaz-Rueda ◽

Manuel Cantos-Barragán ◽

José Manuel Colmenero-Flores

Keyword(s):

Light Intensity ◽

Light Quality ◽

Woody Species ◽

High Sensitivity ◽

Clear Correlation ◽

Light Emitting ◽

Fluorescent Lamps ◽

Light Intensities ◽

Growth Quality

Light-emitting diodes (LEDs) are useful for the in-vitro micropropagation of plants, but little information is available on woody species. This work compares the effects of light quality and intensity on the growth and development of micropropagated olive plants from two different subspecies. Illumination was provided with fluorescent and LED lamps covering different red/blue ratios (90/10, 80/20, 70/30, 60/40) or red/blue/white combinations, as well as different light intensities (30, 34, 40, 52, 56, 84, 98 and 137 µmol m−2 s−1 of photosynthetic photon fluxes, PPF). Olive plants exhibited high sensitivity to light quality and intensity. Higher red/blue ratios or lower light intensities stimulated plant growth and biomass mainly as a consequence of a higher internodal elongation rate, not affecting either the total number of nodes or shoots. In comparison to fluorescent illumination, LED lighting improved leaf area and biomass, which additionally was positively correlated with light intensity. Stomatal frequency was positively, and pigments content negatively, correlated with light intensity, while no clear correlation was observed with light quality. In comparison with fluorescent lamps, LED illumination (particularly the 70/30 red/blue ratio with 34 µmol m−2 s−1 PPF intensity) allowed optimal manipulation and improved the quality of in-vitro micropropagated olive plants.

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Effects of Light Quality on Vegetative Growth and Flower Initiation in Phalaenopsis

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.8632 ◽

2015 ◽

Vol 19 (1) ◽

pp. 33 ◽

Cited By ~ 1

Author(s):

Kumala Dewi ◽

Yekti Asih Purwestri ◽

Yohana Theresia Maria Astuti ◽

Lila Natasaputra ◽

P. Parmi

Keyword(s):

Vegetative Growth ◽

Light Quality ◽

Light Emitting Diodes ◽

Axillary Bud ◽

Growth Chamber ◽

Flower Initiation ◽

Light Emitting ◽

White Leds ◽

Cytokinin Content

The effects of LEDs (Light-Emitting Diodes) emitting different colours namely red, blue, red andblue, and white lights on vegetative growth and fl ower initiation of Phalaenopsis have been evaluated.Phalaenopsis“otohine/taisuco fi re bird” seedlings in vitro were subjected to different light qualities for either2 or 4 weeks, and then each seedling was planted in a plastic pot containing sphagnum and grown in thegrowth chamber under similar light quality for 3 months. For fl ower induction, mature Phalaenopsis plantshaving 4 – 6 leaves were grown for 3 months in the growth chamber under different light qualities. The leafspan, chlorophyll, gibberellin and cytokinin content were determined. In addition, the expressions of FT-likegene in the leaf, axillary bud, fl ower bud and stalk were examined.Vegetative growth was enhanced under blue, red-blue or white LEDs compared to that of the control.Gibberellin and cytokinin content increased in the seedlings subjected to white LEDs. Based on the averageof leaf span increment it was suggested that the growth of Phalaenopsis seedlings can be promoted by givingeither blue, red-blue or white LEDs. From the second experiment, it was found that fl ower induction inPhalaenopsis can be obtained in plants that had just fi nished fl owering without the application of LEDs. Theexpression of FT-like gene in the leaf as well as fl ower bud and stalk suggests that this gene is involved infl ower regulation of Phalaenopsis.

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Clonal Behavior in Response to Soil Water Availability in Tempranillo Grapevine cv: From Plant Growth to Water Use Efficiency

Agronomy ◽

10.3390/agronomy10060862 ◽

2020 ◽

Vol 10 (6) ◽

pp. 862

Author(s):

Ignacio Tortosa ◽

Jose M. Escalona ◽

Guillermo Toro ◽

Cyril Douthe ◽

Hipolito Medrano

Keyword(s):

Plant Growth ◽

Water Use Efficiency ◽

Soil Water ◽

Water Use ◽

Water Availability ◽

Growth Parameters ◽

Biomass Accumulation ◽

Net Photosynthesis ◽

Soil Water Availability ◽

Use Efficiency

Climate change threatens worldwide grapevine production, especially in Mediterranean areas. To assume this challenge, the replacement of plant material to choose one more adapted to the new environmental conditions has been proposed as one of the possible solutions. Thus, the study of genetic variability in water-use efficiency (WUE) is needed to guarantee the survival of viticulture in those critical areas. In this study, the variability of WUE in 23 Tempranillo clones growth in pots and submitted to well-watered conditions and moderate water stress was studied along two consecutive years. Leaf net photosynthesis (AN), stomatal conductance (gs), and plant growth parameters were measured, in addition the instrisic WUE (AN/gs), biomass production, and water consumed were calculated. Results show a clear genotype effect for most of the studied parameters, but, with an important year by year variability. We identified different clonal behavior in response to soil water availability, that permits to classify them as water-savers vs water-spenders. In general, there was no found relationship between leaf and plant WUE, even some genotypes as 232 or 1048 were coincident in higher leaf WUE showed highly productive in terms of biomass accumulation per unit of water applied.

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Comparisons of Different Lighting Systems for Horticultural Seedling Production Aimed at Energy Saving

Sustainability ◽

10.3390/su10093351 ◽

2018 ◽

Vol 10 (9) ◽

pp. 3351 ◽

Cited By ~ 7

Author(s):

Pedro Garcia-Caparros ◽

Rosa Chica ◽

Eva Almansa ◽

Antonio Rull ◽

Lara Rivas ◽

...

Keyword(s):

Production Systems ◽

Tomato Seedling ◽

Light Emitting ◽

White Leds ◽

Technical Parameters ◽

Light Irradiance ◽

Fluorescent Lamps ◽

The Status ◽

Total Irradiance ◽

Lighting Systems

Nowadays, the evaluation of sustainability is an important aspect in the study of agricultural systems and the number of projects and methods for impact assessment of food production systems is increasing. In this work, we initially carried out a survey to know the status of the artificial lighting establishment in horticultural seedling nurseries in southern Spain. Taking into account the data obtained in the survey, we conducted an experiment with different types of fluorescent lamps (TLD-18, CF, TL5, TLD-56), light-emitting diodes (LEDs) and their combinations along with the novelty white LEDs lamps and XTRASUN LEDs to evaluate their technical parameters and spectral light qualities. In addition, the effectiveness of light irradiance (ELIplant) and the use of irradiance (UI) by cucumber and tomato seedling plants were estimated considering their light absorbance capacity previously analyzed. The results showed that TLD-18 lamps and their combinations, CF and XTRASUN LEDs, had a limited value of energy efficiency (VEEI) ≤ 2. The lamps essayed with the lowest total irradiance were LEDs (B, R, V, W) and the ones with the highest values were TLD58-6 lamps. The effectiveness of light irradiance (ELIplant) and the UI were slightly higher in the case of cucumber than that of the tomato for all essayed lamps. Considering the effectiveness of the light irradiance (ELIplant), TL5-6 lamps showed the highest values. On the other hand, considering the use of irradiance, XTRASUN LEDs on the mode of vegetative growth (VG) showed the highest values.

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Competitive attributes ofA. sativa, T. aestivum, andH. vulgareare conserved in no-till cropping systems

Weed Science ◽

10.1017/s0043174500091384 ◽

1999 ◽

Vol 47 (6) ◽

pp. 712-719 ◽

Cited By ~ 3

Author(s):

Anne Légère ◽

Yuguang Bai

Keyword(s):

Hordeum Vulgare ◽

Avena Sativa ◽

Growth Parameters ◽

Cropping Systems ◽

Seedling Emergence ◽

Biomass Accumulation ◽

Tillage Systems ◽

Area Index ◽

No Till ◽

Lower Test

The robustness of competitive attributes of cereals such as rapid and uniform seedling emergence, tillering, early biomass accumulation and canopy closure, and height advantage over weeds have not yet been tested under environmental conditions typical of no-till (NT) cropping systems. Our objective was to evaluate the effects or NT practices on growth and productivity ofAvena sativa, Triticum aestivum, Hordeum vulgare, and associated weeds. The experiment was conducted on a Kamouraska clay at La Pocatière, QC, in 1994, 1995, and 1996.Avena sativa, T. aestivum, andH. vulgarewere grown under tilled and NT practices. Cereal growth parameters were measured six (1994) or seven (1995) times between planting and the 11th week after planting but only once in 1996. Grain yields and yield components were determined at crop maturity.Avena sativaandH. vulgarepopulations were little affected by tillage, whereasT. aestivumpopulations were reduced by 16 to 20% in NT systems. Growth in height in NT systems was either similar or greater than in tilled systems in all three cereals. Cereal leaf area index (LAI) and biomass accumulation was also comparable between tillage systems, except forT. aestivumLAI in 1994, which was greater in tilled plots on two sampling dates. Response of annual dicots to tillage was inconsistent in all crops. Annual monocots dominated in some but not all NT systems. Perennial dicots dominated in NT systems, whereas perennial monocots were more abundant in tilled systems in all three cereals.Avena sativaandT. aestivumyields in NT plots were comparable or greater than in tilled plots, in spite of having either lower test weights (A. sativa) or lower 1,000-grain weights (T. aestivum). NTT. aestivumproductivity was maintained in spite of reduced plant establishment.Hordeum vulgareyields were also similar across tillage systems, except in 1995, when yields in tilled plots were greater than in NT plots. The height advantage observed for NTH. vulgaredid not result in improved yields. All three cereals, and particularlyA. sativa, appeared well suited to NT systems, despite the pressure provided by different weed groups, compared to tilled systems. However, results suggest that NT production of cereals could benefit from improved attention to perennial dicot control and crop seedling establishment, particularly forT. aestivum.

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