scholarly journals Effect of eugenol on hematological parameters in rats

2013 ◽  
Vol 67 (1-2) ◽  
pp. 29-42
Author(s):  
Milanka Jezdimirovic ◽  
Nevenka Aleksic ◽  
Mirjana Milovanovic ◽  
Dragica Stojanovic ◽  
Nemanja Jezdimirovic ◽  
...  
Keyword(s):  
Significant Influence ◽  
Hemolytic Activity ◽  
Hematological Parameters ◽  
Haemoglobin Concentration ◽  
Control Group ◽  
Duration Of Treatment ◽  
Volume Number ◽  
The Absolute ◽  
Absolute Control ◽  
Different Doses

Investigations covered the possible hematotoxic effect of eugenol in rats following two-week and four-week continuous p.o. application. An experiment was conducted on 72 maleWistar rats divided into six groups. Four groups were treated with different doses of eugenol (10 mg/kg bm/day, 50 mg/kg/day, 200 mg/kg/day and 400 mg/kg bm/day), the control group was administered a vehiculum (0,5 % methylcellulose, 20 % propylene glycol and water), and the sixth group was the absolute untreated control. Eugenol and the vehiculum were administered using a gastric probe in a volume of 1 ml/100 g body mass of rat. Blood was sampled using cardiac puncture on days 14 and 28 of the experiment in order to determine hematological parameters (hematocrit, number of erythrocytes, MCV, haemoglobin concentration, MCH, number of leukocytes, leukocyte formula, and number of thrombocytes). The results have shown that eugenol administered over 14 and 28 days in doses of 10, 50, 200 i 400 mg/kg bm/day has no hemolytic activity. Furthermore, administered over four weeks, it does not significantly affect the number of erythrocytes, haemoglobin concentration, hematocrit, erythrocyte volume, number of leukocytes, and the leukocyte formula. Applied over two weeks, eugenol causes a significant increase in the mass of haemoglobin per erythrocyte, in comparison with controls. This effect is non-specific and does not depend on the dose or on the duration of treatment. The eugenol doses of 10 and 200 mg/kg/day administered over a period of four weeks result in a statistically significant reduction in the number of thrombocytes in comparison with the absolute control, while the highest investigated dose (400 mg/kg) causes a significant increase in comparison with the numbers for rats treated with a dose of 10 and 200 mg/kg/day. The changes in thrombocyte number caused by eugenol are qualitatively different and depend neither on the dose nor on the duration of treatment. Eugenol applied over two or four weeks does not exhibit a significant influence on the number of lymphocytes, monocytes or granulocytes.

scholarly journals ANALYSIS OF HEMATOLOGICAL PARAMETERS IN PATIENTS WITH ISCHEMIC STROKE AT DMCH, LAHERIASARAI, BIHAR

2020 ◽  
pp. 49-51
Author(s):  
Megha Jha ◽  
Hari Shankar Mishra ◽  
Ajit Kumar Chaudhary ◽  
Debarshi Jana
Keyword(s):  
Ischemic Stroke ◽  
Hematological Parameters ◽  
Local Population ◽  
Haemoglobin Concentration ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Stroke Patients ◽  
Cross Sectional ◽  
Stroke Incidence ◽  
Significant Difference

Background: Hematological parameters are major indicator of ischemic stroke. Hematological parameters and stroke incidence have not been investigated in local population. Objective: The present study was designed to assess whether hematological parameters play an important role in ischemic stroke patients. Methodology: For this purpose a cross-sectional study was done at Department of Pathology,Darbhanga Medical College andHospital, Laheriasarai, Bihar from January 2020 to August 2020. A total of 150subjects were enrolled in Medicine Department and referred to Pathology Department for various hematological test.Patients divided into two groups, control (n=50) and ischemic stroke subjects (n=100). Data regarding risk factors was collected through questionnaire. Their hematological parameters such as WBCs count, RBCs count, haemoglobin concentration, HCT, MCV, MCH and PLT count were assessed by hematology analyzer. Statistical analysis was done by using SPSS (version 13). Result: Mean age of control and ischemic stroke group was 61.40y±1.33 and 63.8y±1.36 respectively with non-significant difference between the groups. Among the ischemic stroke subjects, 73% of the subjects were suffering from hypertension, 53% reported diabetes, 41% had heart disease, 39% had family history of stroke, 32% were smokers and 46% use high cholesterol food. The WBCs count, MCV, MCH and HCT were found significantly higher in ischemic stroke subjects when compared with control group while the significant decrease was observed in RBCs count and hemoglobin concentration. PLT count increased in ischemic stroke subjects as compared to control group. Conclusion: It was concluded that in ischemic stroke patients hematological parameters were greatly altered.

Effect of microclimatic variables on physiological and hematological parameters of crossbred cows in summer season

2017 ◽  
Author(s):  
Ranjana Sinha ◽  
Madan Lal Kamboj ◽  
Ashish Ranjan ◽  
Indu Devi
Keyword(s):  
Dry Matter ◽  
Hematological Parameters ◽  
Haemoglobin Concentration ◽  
Summer Season ◽  
Dry Matter Intake ◽  
Haematological Parameters ◽  
Maximum Temperature ◽  
Control Group ◽  
Mosquito Net ◽  
Crossbred Cows

The present investigation was conducted to study the effect of climatic variables on physiological responses and haematological parameters of crossbred cows in summer season. In this study 16 crossbred cows were selected and randomly divided into two groups (G1 and G2). Cows of control group were housed in shed with sprinkler, whereas the cows of treatment group were housed in shed with fogger plus fans and mosquito net. Data on various physiological and haematological parameters were recorded and analysed statistically. Analysis revealed that, the maximum temperature and THI were significantly (P less than 0.05) lower in treatment shed as compared to control shed. Significantly (P less than 0.05) higher dry matter intake and milk yield in treatment (13.64±0.44 and 18.18±0.36 kg) as compared to control group (12.33±0.37 and 15.94±0.47 kg) were recorded during peak hot period. Rectal temperature, respiration rate and pulse rate was significantly (P less than 0.05) lower in treatment (38.28±0.05, 36.42±2.98 and 78.65±1.09 °C) as compared to control group (38.57±0.14, 51.15±4.34 and 83.59±0.83 °C). However, haemoglobin concentration (g/dl) and white blood corpuscles (103/cmm) was significantly (P less than 0.05) higher in treatment (10.54±0.36 and 12.35±0.64) as copared to control group (9.52±0.43 and10.88±0.57). Therefore, it can be concluded that fogger with fans is the fundamental mean to protect the animals from thermal stress under loose housing during summer season.

scholarly journals Correlations among Vetom 21.77 doses, mass and some   hematological parameters of broilers

2018 ◽  
Vol 48 (3) ◽  
pp. 92-102
Author(s):  
E. R. Rafikova ◽  
G. A. Nozdrin ◽  
A. A. Leliak
Keyword(s):  
Body Weight ◽  
Final Stage ◽  
Hematological Parameters ◽  
Digital Data ◽  
Control Group ◽  
Absolute Weight ◽  
Live Body Weight ◽  
The Absolute

The research aims at revealing possible correlations among the dose of microbiological specimen vetom 21.77, body weight and concentration of erythrocytes and hematoglobulin in the poultry blood which received the specimen. The researchers arranged 5 groups: the control group and 4 experimental ones. Each group contained 20 broilers. The researchers used 5 chickens additionally in order to define critical hematological parameters before applying the specimens in the experimental groups. The specimen was applied when feeding the poultry from 1-4 experimental groups orally once a day dosed 2, 5, 50 and 300 μl / kg of weight, respectively, with food and water for 7 days. The chickens from the control group didn’t get the specimen. When analyzing the results obtained,  the digital data was visualized using the swing diagrams and correlation diagrams in order to detail the observed tendencies. At the final stage of the experiment, the average absolute weight of broilers which received vetom 21..77 in the investigated doses was on 0.2-1.4% higher in comparison with the same parameter in the control group. Median parameters   of the absolute  poultry weight which received the specimen dosed 50 and 300 μl / kg of body weight, were on 1.4 and 1.9% higher in the control group. The number of erythrocytes in the poultry blood which received vetom 21.77 at a dose of 300 μl / kg of mass was higher at the end of the experiment than in the control group on 6.3% (P <0.05). The median of the sample exceeded the median of the control group on 8.9%. The average parameters   of hematoglobulin concentration in the blood of chickens which  received the specimen at doses of 50 and 300 μl / kg of mass were on 3.4 (P <0.01) and 3.8% (P <0.01) higher. Medians of these groups were higher than those in the control group on 5%. The article highlights the correlations between the dose of the specimen and live body weight (P <0.001), and between the concentration of erythrocytes (P 0.001) and hematoglobulin (P 0.001) in the blood of experimental poultry.

scholarly journals The Modulatory Effects of Pentoxifylline in Biochemical Changes Induced By 17α-Ethinyl Estradiol in the Rat Model

2018 ◽  
Vol 12 (4) ◽  
pp. 5-9
Author(s):  
Aysen Kor ◽  
◽  
Ebrahim Shahroozian ◽  
Ahmadi-Hamedani Mahmood ◽  
Saeideh Naeimi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normal Saline ◽  
Ethinyl Estradiol ◽  
Hematological Parameters ◽  
Serum Levels ◽  
Treated Group ◽  
Control Group ◽  
Acid Uptake ◽  
Duration Of Treatment ◽  
Tissue Samples

Background: Ethinylestradiol (EE) has induced cholestasis and hepatotoxicity in animal studies through reducing bile acid uptake by hepatocytes and induce of oxidative stress. Pentoxifylline (PTX) is a drug that by inhibition of release or transcription of proinflammatory cytokine cause prevents oxidative stress of liver cell and reduction of damage. We aimed to evaluate the effects of pentoxifylline on liver injury induced by Ethinylestradiol in rats. Methods: Twenty-four female Wistar rats (300±20 gr) were divided into four groups, equally. Duration of treatment was 5 consecutive days for each group. The control group Simultaneously received orally and subcutaneously normal saline. PTX group Simultaneously received Pentoxifylline orally and normal saline subcutaneously, EE Group Simultaneously received EE subcutaneously and normal saline orally. In the EE+PTX group, rats Simultaneously received EE subcutaneously and PTX orally. Rats were anesthetized and blood and tissue samples were collected for measurement of hematological and biochemical parameters. Results: The EE administration increased the serum levels of ALP and MDA significantly. The EE administration also decreased albumin and GPX levels were significant. These aberrations were improved by PTX treatment in EE + PTX group. Most of hematological parameters were not significant by the EE. The plasma level of TNF- in the PTX+ES treated group showed a significant decrease in comparison to that in the ethinyl estradiol group. Conclusion: PTX has partial capacity to protect against liver changes induced by Ethinyl Estradiol.

EVALUATION OF HEMATOLOGICAL PARAMETERS IN ALBINO RATS EXPOSED DERMALLY TO TANNERY EFFLUENT FROM CHALLAWA INDUSTRIAL ESTATE KANO, NIGERIA.

2021 ◽  
Vol 5 (2) ◽  
pp. 372-376
Author(s):  
K. A. Bichi ◽  
B. B. Yusif ◽  
S. B. Mustapha ◽  
Y. U. Jibrin ◽  
P. S. Thomas
Keyword(s):  
Blood Cell ◽  
Hematological Parameters ◽  
Haemoglobin Concentration ◽  
Physicochemical Analysis ◽  
Control Group ◽  
Albino Rats ◽  
Tannery Effluent ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Standard Limit

The effects on haematological parameters of albino rats were evaluated to assess possible toxicity of tannery effluent. The study was carried out for 28 days. Forty (40) matured albino rats of both sexes were divided into four groups of 10 rats each. Group 1 is the control group, which were exposed dermally with normal water. Groups 2, 3 and 4 were exposed dermally at different concentrations of 100%, 50% and 25% of the effluent respectively. The result of the physicochemical analysis of the effluent shows that the physicochemical parameters were above the standard limit. Rats exposed to different percentages of the effluent showed significant decrease in the level of haemoglobin and packed cells volume. Red blood cell, white blood cell, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelet and lymphocytes do not significantly decrease or increase.

scholarly journals MORPHOLOGICAL COMPOSITION AND PROFILE ACTIVITY OF T- AND B-LYMPHOCYTES OF BLOOD OF DRY-PROOF COWS WITH THE INTRODUCTION OF VARIOUS DOSES OF TISSUE BIOSTIMULATOR

2020 ◽  
Author(s):  
И.А. ПУШКАРЕВ ◽  
Т.В. Куренинова ◽  
Н.В. Шаньшин ◽  
А.И. Афанасьева
Keyword(s):  
B Lymphocytes ◽  
Physiological Saline ◽  
Optimal Dose ◽  
Absolute Number ◽  
T And B Lymphocytes ◽  
Control Group ◽  
Absolute Amount ◽  
Dry Period ◽  
The Absolute ◽  
Different Doses

В статье приведены результаты исследований, целью которых являлось изучение влияния введения разных доз тканевого биостимулятора на пролиферативную активность Т- и В-систем иммунной системы сухостойных коров. Экспериментальные исследования проведены в 2019 г. в производственных условиях АО «Учхоз Пригородное» Индустриального района г. Барнаула на коровах приобского типа черно-пестрой породы, на последних месяцах стельности. Нами сформированы 4 подопытные группы животных с учетом возраста и молочной продуктивности, предшествовавших сухостойному периоду. Тканевой биостимулятор вводился за 55-60 дней до предполагаемого отела, четырехкратно с интервалом в 14 дней, в разных дозах: коровам опытной группы I – 15 мл/гол., II – 22,5 мл/гол., III – 30 мл./гол. Животным контрольной группы вводили физиологический раствор в дозе 22,5 мл./гол. Опытная партия тканевого биостимулятора изготовлена из субпродуктов и боенских отходов пантовых оленей по запатентованной технологии. В ходе исследований установлено, что введение разных доз тканевого биостимулятора способствует увеличению некоторых гематологических показателей и пролиферативной активности Т- и В-лимфоцитов крови сухостойных коров. Оптимальной дозой применения тканевого биостимулятора является 22,5 мл/гол, что способствует повышению содержания в крови сухостойных коров эритроцитов на 11,1 % (p≤0,05), относительного количества рЕ-РОК – на 7,4 % (p≤0,05), ЕМ-РОК – на 9,6 % (p≤0,01), абсолютного количества бЕ-РОК – на 50,0 % (p≤0,05) и ЕМ-РОК – на 83,0 % (p≤0,01), коэффициента абсолютного числа «индукторов-хелперов» и «киллеров-супрессоров» в крови – на 53,8 % (p≤0,05) относительно показателей, установленных у животных контрольной группы. The article presents the results of studies whose purpose was to study the effect of the introduction of different doses of tissue biostimulant on the prophylactic activity of T- and B-systems of immunity of dry cows. The experiment was carried out in 2019 under the production conditions of Uchkhoz Prigorodnoye JSC in the Industrial District of Barnaul on cows of the acquired type of black-motley breed during the dry period. We have formed 4 experimental groups of animals. In the selection, which took into account the age and milk productivity preceding the dry period. Tissue biostimulant was administered during dead time 55-60 days before the expected calving, four times with an interval of 14 days, in different doses in the experimental I - 15 ml / goal., In II – 22,5 ml / goal, in III - 30 ml / goal. In the control, physiological saline was used at a dose of 22,5 ml / goal. An experimental batch of tissue biostimulator was made from offal and slaughter reindeer waste using a patented technology. In the course of the experiment, it was found that the introduction of different doses of tissue biostimulant increases the proliferative activity of blood T and B lymphocytes of dry cows. However, the most optimal dose for using a tissue biostimulant is 22,5 ml / goal, which contributes to the greatest increase in the blood content of dry cows of erythrocytes by 11,1% (p≤0,05) the relative amount of pE-ROCK by 7,4% (p≤0,05), EM-ROCK by 96% (p≤0,01), the absolute amount of BE-ROCK by 50,0% (p≤0,05) and EM-ROCK by 80,0% (p≤0,01), the largest increase in the absolute number coefficient of “inductors- helpers ”and“ killer-suppressors ”in the blood by 53,8% (p≤0,05), relative to indicators established in animals of the control group.

scholarly journals Analysis of hematological parameters in patients with ischemic stroke

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Saima Sharif
Keyword(s):  
Ischemic Stroke ◽  
Hematological Parameters ◽  
Local Population ◽  
Haemoglobin Concentration ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Stroke Patients ◽  
Cross Sectional ◽  
Stroke Incidence ◽  
Significant Difference

Background: Hematological parameters are major indicator of ischemic stroke. Hematological parameters and stroke incidence have not been investigated in local population. Objective: The present study was designed to assess whether hematological parameters play an important role in ischemic stroke patients. Methodology: For this purpose a cross-sectional study was done at Services hospital, Lahore from January 2013 to August 2013. A total of 150subjects were enrolled and divided into two groups, control (n=50) and ischemic stroke subjects (n=100). Data regarding risk factors was collected through questionnaire. Their hematological parameters such as WBCs count, RBCs count, haemoglobin concentration, HCT, MCV, MCH and PLT count were assessed by hematology analyzer. Statstical analysis was done by using SPSS (version 13). Result: Mean age of control and ischemic stroke group was 61.40y±1.33 and 63.8y±1.36 respectively with non-significant difference between the groups. Among the ischemic stroke subjects, 73% of the subjects were suffering from hypertension, 53% reported diabetes, 41% had heart disease, 39% had family history of stroke, 32% were smokers and 46% use high cholesterol food. The WBCs count, MCV, MCH and HCT were found significantly higher in ischemic stroke subjects when compared with control group while the significant decrease was observed in RBCs count and hemoglobin concentration. PLT count increased in ischemic stroke subjects as compared to control group. Conclusion: It was concluded that in ischemic stroke patients hematological parmeters were greately altered.

Reasoning of medical tactics at the preconception in patients with a history of miscarriage and abnormal vaginal discharge

2016 ◽  
pp. 52-56
Author(s):  
N.V. Schuruk ◽  
◽  
V.I. Pyrohova ◽  
Keyword(s):  
Vaginal Discharge ◽  
Patient Survey ◽  
Economic Benefits ◽  
Control Group ◽  
Positive Trend ◽  
Duration Of Treatment ◽  
Genital Infections ◽  
Reproductive Losses ◽  
Abnormal Vaginal Discharge ◽  
Complicated Pregnancy

The objective: evaluating the effectiveness and safety of the drug complex hinekit during preconception preparation for women with complicated pregnancy loss history and uncomplicated mixed genital infections. Patients and methods. The study involved 65 women with reproductive losses in history, who turned over the abnormal vaginal discharge. Diagnosis of sexual transmitted infections (C. trachomatis, T. vaginalis, HSV-II, HPV), assessment of vaginal microbiota was performed by PCR with detection results in real time. Bacterial Vaginosis verified by the Amsel system. Results. All the examinees in the history have miscarriage in the first (56.9%) or the second (43.1%) trimester of pregnancy. According to the results of the patient survey were divided into clinical groups. In 26.2% of women infectious pathology of the lower genital tract has not been confirmed, 26.2% of patients did not give consent to participate in the study and were formed in the control group that received therapy according to existing clinical settings. 31 patients with mixed genital infection (study group) received complex preparation gіnekit. This positive trend was observed in all the patients of the main group (the disappearance of subjective sensations on average 2.2±0.3 hours). After treatment T. vaginalis, BV were not detected in one patient, active mycotic process in the absence of complaints remained at 4.8% of women, complete eradication of C. trachomatis was achieved in 92.3% of patients. Conclusions. Reducing the duration of treatment of mixed genital infections at the preconception in women with reproductive losses in the history is achieved in terms of clinical, microbiological and pharmaco-economic benefits by prescribtion an gіnekit preparation. Summary of clinical and microbiological effects of the proposed approach is 96.0 ± 0.9%, which corresponds to all requirements for multimodal therapy of genital infections, and no side effects, short course of treatment provides a high compliance of patients (9.2±0.7 points). Key words: mixed genital infections, miscarriages, gіnekit, azithromycin, fluconazole, seknidazol.

Erythrocyte and reticulocyte parameters during biological monitoring of lead exposure to the body

2020 ◽  
Vol 60 (11) ◽  
pp. 873-876
Author(s):  
Anastasiya G. Khotuleva ◽  
Mariya S. Kozyreva
Keyword(s):  
Biological Monitoring ◽  
Hematological Parameters ◽  
Lead Level ◽  
Statistical Processing ◽  
Absorption Spectrometry ◽  
The Body ◽  
Control Group ◽  
Processing Plant ◽  
Hematological Disorders ◽  
Globin Synthesis

Introduction. The most susceptible to lead is the hematopoietic system of hematopoietic organs due to lead inhibition of heme and globin synthesis and cytotoxic effect on the membrane of Mature red blood cells. The aim of study was to evaluate the informative value of the study of erythrocyte and reticulocyte parameters determined on modern hematological analyzers in patients working in contact with lead during medical and biological monitoring. Materials and methods. 45 employees of the lead battery processing plant and 30 persons of control group were examined. The level of lead in the blood was determined by atomic absorption spectrometry, δ-ALA in the urine-by the reaction of pyrol formation with acetylacetone in terms of gram of creatinine, the study of hematological parameters was performed on a Sysmex HT-2000i analyzer. Statistical processing of the results was performed using the program STATISTICA 10.0. Results. Significant changes in erythrocytic (RDW) and reticulocytic (RET, IRF, LFR, MFR, HFR, RET-He) parameters, erythropoietin in workers in contact with lead compared to the control group, changes in MCV, MCH, RDW, RET indicators in the group working in dynamics after 2 years were revealed. Associations of hematological parameters with biomarkers of exposure and effect (lead level in blood and ALA in urine) were revealed. Conclusions. Assessment of erythrocyte (MCV, MCH, RDW) and reticulocyte parameters (RET% and their distribution by maturity) in dynamics during periodic medical examinations of workers in contact with lead allows us to detect the development of hematological disorders at early stages.

Probiotics in the diets of laying quails

2020 ◽  
pp. 60-66
Author(s):  
O. Merzlyakova ◽  
V. Rogachyev ◽  
V. Chegodaev
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Egg Production ◽  
Positive Impact ◽  
Hematological Parameters ◽  
Economic Effect ◽  
Egg Laying ◽  
Control Group ◽  
Egg Mass ◽  
The Cost

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).

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